SSGCID: Target Page

MysmA.00414.c

Short-chain dehydrogenase/reductase SDR

CENTER ID:	MysmA.00414.c
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00414.c.A1.GE28636	Full length( MysmA.00414.c )		1	265

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.2881
RefSeq:	YP_887237.1
UniProt:	A0QWE9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDLQLTGKRA IITGGSKGIG LAIAHALATE GVDVVLAARN QETLEKAAAE VAERTGRRAI PVVADTGDDE SVKALVARAV EELGGVDILV NNASNQAVGA AFPDLAGTTD EAFWGDVNVK VVGYLRAARA VAPHLKAQGW GRIINISGLG YWQTHSIVRS IRNVSVAALT KNLADELGPH GINVTVVHPG QTRTESKTAQ FEAAARAAGI DVADYERQIA DNAIGRVVDA AEVADVVAFL ASPRSVSITG DAVAVGGGLL GTVNY

NT Sequence

ATGGATCTGC AACTGACCGG CAAGCGCGCG ATCATCACCG GCGGCAGCAA AGGCATCGGC CTGGCGATCG CCCACGCGCT GGCCACCGAA GGCGTCGACG TGGTGCTCGC CGCGCGCAAT CAGGAGACCC TGGAGAAGGC CGCCGCCGAG GTCGCCGAAC GCACCGGGCG GCGGGCGATC CCCGTCGTCG CCGACACCGG GGACGACGAG TCGGTCAAGG CCCTCGTCGC TCGTGCCGTC GAGGAACTCG GCGGCGTCGA CATCCTGGTC AACAACGCAT CGAATCAGGC GGTCGGCGCG GCGTTCCCGG ATCTGGCGGG CACCACCGAC GAGGCGTTCT GGGGCGACGT CAACGTGAAG GTCGTGGGCT ATCTGCGCGC CGCGCGCGCC GTCGCGCCGC ATCTCAAGGC GCAAGGTTGG GGCCGCATCA TCAACATCAG CGGGCTCGGC TACTGGCAGA CCCATTCGAT CGTGCGCAGC ATCCGTAACG TCAGTGTGGC GGCGCTGACC AAGAACCTCG CCGACGAACT CGGACCGCAC GGGATCAACG TCACCGTCGT GCATCCCGGG CAGACGCGCA CCGAGAGCAA GACCGCCCAG TTCGAGGCCG CGGCCAGGGC CGCCGGGATC GATGTCGCCG ACTACGAGCG GCAGATCGCC GACAACGCCA TCGGGCGGGT CGTGGATGCC GCGGAAGTGG CCGATGTCGT GGCCTTTCTC GCCTCGCCGC GCAGCGTGAG CATCACCGGG GACGCCGTGG CCGTGGGCGG TGGCCTGCTC GGCACCGTCA ATTAC

Details for MysmA.00414.c.A1.GE28636

HARVESTED ON:	2/17/2010
SEQUENCED ON:	2/24/2010
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDLQLTGKR AIITGGSKGI GLAIAHALAT EGVDVVLAAR NQETLEKAAA EVAERTGRRA IPVVADTGDD ESVKALVARA VEELGGVDIL VNNASNQAVG AAFPDLAGTT DEAFWGDVNV KVVGYLRAAR AVAPHLKAQG WGRIINISGL GYWQTHSIVR SIRNVSVAAL TKNLADELGP HGINVTVVHP GQTRTESKTA QFEAAARAAG IDVADYERQI ADNAIGRVVD AAEVADVVAF LASPRSVSIT GDAVAVGGGL LGTV

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggatc tgcaactgac cggcaagcgc gcgatcatca ccggcggcag caaaggcatc ggcctggcga tcgcccacgc gctggccacc gaaggcgtcg acgtggtgct cgccgcgcgc aatcaggaga ccctggagaa ggccgccgcc gaggtcgccg aacgcaccgg gcggcgggcg atccccgtcg tcgccgacac cggggacgac gagtcggtca aggccctcgt cgctcgtgcc gtcgaggaac tcggcggcgt cgacatcctg gtcaacaacg catcgaatca ggcggtcggc gcggcgttcc cggatctggc gggcaccacc gacgaggcgt tctggggcga cgtcaacgtg aaggtcgtgg gctatctgcg cgccgcgcgc gccgtcgcgc cgcatctcaa ggcgcaaggt tggggccgca tcatcaacat cagcgggctc ggctactggc agacccattc gatcgtgcgc agcatccgta acgtcagtgt ggcggcgctg accaagaacc tcgccgacga actcggaccg cacgggatca acgtcaccgt cgtgcatccc gggcagacgc gcaccgagag caagaccgcc cagttcgagg ccgcggccag ggccgccggg atcgatgtcg ccgactacga gcggcagatc gccgacaacg ccatcgggcg ggtcgtggat gccgcggaag tggccgatgt cgtggccttt ctcgcctcgc cgcgcagcgt gagcatcacc ggggacgccg tggccgtggg cggtggcctg ctcggcaccg tca

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatctgcaa ctgaccggca agcgcgcgat catcaccggc ggcagcaaag gcatcggcct ggcgatcgcc cacgcgctgg ccaccgaagg cgtcgacgtg gtgctcgccg cgcgcaatca ggagaccctg gagaaggccg ccgccgaggt cgccgaacgc accgggcggc gggcgatccc cgtcgtcgcc gacaccgggg acgacgagtc ggtcaaggcc ctcgtcgctc gtgccgtcga ggaactcggc ggcgtcgaca tcctggtcaa caacgcatcg aatcaggcgg tcggcgcggc gttcccggat ctggcgggca ccaccgacga ggcgttctgg ggcgacgtca acgtgaaggt cgtgggctat ctgcgcgccg cgcgcgccgt cgcgccgcat ctcaaggcgc aaggttgggg ccgcatcatc aacatcagcg ggctcggcta ctggcagacc cattcgatcg tgcgcagcat ccgtaacgtc agtgtggcgg cgctgaccaa gaacctcgcc gacgaactcg gaccgcacgg gatcaacgtc accgtcgtgc atcccgggca gacgcgcacc gagagcaaga ccgcccagtt cgaggccgcg gccagggccg ccgggatcga tgtcgccgac tacgagcggc agatcgccga caacgccatc gggcgggtcg tggatgccgc ggaagtggcc gatgtcgtgg cctttctcgc ctcgccgcgc agcgtgagca tcaccgggga cgccgtggcc gtgggcggtg gcctgctcgg caccgtcaat tacaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa