MysmA.00358.o

Probable enoyl-CoA hydratase

CENTER ID: MysmA.00358.o
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00358.o.A1.GE28626 Full length( MysmA.00358.o ) 1 235
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00358.o.A1.PS00593 Full length( MysmA.00358.o ) 1 235

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.2293
RefSeq: YP_885445.1
UniProt: A0QRA7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNEFIKIVLG SAPEQTGVAT LLLSRPPTNA LTRQMYREIS IAANELAQRA DVSSVIVYGG HEIFCAGDDI PELRTLDAEE TAAADHALQR CIEAVAAIPK PTVAAVTGYA LGSGMNLALA ADWRVSGDNA KFGATEILAG LAPRGGGGVR LADAIGTSKA KELVFSGRFV GAEEALEIGL VDEMVAPDHV YEAALAWARR FSDHPVDVLA AAKASLNGPV NWRGPRPTDR MVDRP
NT Sequence
ATGAACGAGT TCATCAAGAT CGTCCTCGGT TCCGCGCCCG AGCAGACGGG CGTGGCCACG TTGCTGTTGT CGCGGCCCCC GACGAACGCG TTGACCCGCC AGATGTACCG CGAGATCTCC ATCGCCGCGA ACGAGCTCGC CCAGCGCGCC GACGTGTCGT CGGTCATCGT CTACGGCGGC CACGAGATCT TCTGCGCGGG CGACGACATC CCCGAACTGC GCACCCTCGA CGCCGAAGAG ACCGCGGCGG CCGACCACGC GCTGCAGCGC TGCATCGAGG CCGTTGCCGC CATCCCCAAA CCCACGGTCG CGGCCGTCAC CGGGTACGCG CTCGGCAGCG GGATGAACCT GGCGCTGGCC GCGGACTGGC GGGTGAGCGG CGACAACGCC AAGTTCGGCG CCACCGAGAT CCTGGCGGGG CTGGCACCGC GCGGCGGCGG CGGGGTACGC CTGGCGGACG CCATCGGGAC CAGCAAGGCC AAGGAGCTCG TGTTCAGCGG CCGGTTCGTC GGCGCCGAGG AAGCGCTCGA GATCGGCCTG GTCGACGAGA TGGTGGCACC CGACCACGTC TACGAGGCCG CGCTCGCGTG GGCCCGCCGG TTCTCCGACC ATCCGGTCGA CGTGCTGGCC GCCGCGAAAG CCTCCCTCAA CGGGCCGGTC AACTGGCGGG GCCCCCGCCC GACCGATCGG ATGGTGGACC GGCCC
Details for MysmA.00358.o.A1.GE28626
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 27kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNEFIKIVL GSAPEQTGVA TLLLSRPPTN ALTRQMYREI SIAANELAQR ADVSSVIVYG GHEIFCAGDD IPELRTLDAE ETAAADHALQ RCIEAVAAIP KPTVAAVTGY ALGSGMNLAL AADWRVSGDN AKFGATEILA GLAPRGGGGV RLADAIGTSK AKELVFSGRF VGAEEALEIG LVDEMVAPDH VYEAALAWAR RFSDHPVDVL AAAKASLNGP VNWRGPRPTD RMV
Validated NT Sequence
ccaccatccg atcggtcggg cgggggcccc gccagttgac cggcccgttg agggaggctt tcgcggcggc cagcacgtcg accggatggt cggagaaccg gcgggcccac gcgagcgcgg cctcgtagac gtggtcgggt gccaccatct cgtcgaccag gccgatctcg agcgcttcct cggcgccgac gaaccggccg ctgaacacga gctccttggc cttgctggtc ccgatggcgt ccgccaggcg taccccgccg ccgccgcgcg gtgccagccc cgccaggatc tcggtggcgc cgaacttggc gttgtcgccg ctcacccgcc agtccgcggc cagcgccagg ttcatcccgc tgccgagcgc gtacccggtg acggccgcga ccgtgggttt ggggatggcg gcaacggcct cgatgcagcg ctgcagcgcg tggtcggccg ccgcggtctc ttcggcgtcg agggtgcgca gttcggggat gtcgtcgccc gcgcagaaga tctcgtggcc gccgtagacg atgaccgacg acacgtcggc gcgctgggcg agctcgttcg cggcgatgga gatctcgcgg tacatctggc gggtcaacgc gttcgtcggg ggccgcgaca acagcaacgt ggccacgccc gtctgctcgg gcgcggaacc gaggacgatc ttgatgaact cgttcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNEFIKIVL GSAPEQTGVA TLLLSRPPTN ALTRQMYREI SIAANELAQR ADVSSVIVYG GHEIFCAGDD IPELRTLDAE ETAAADHALQ RCIEAVAAIP KPTVAAVTGY ALGSGMNLAL AADWRVSGDN AKFGATEILA GLAPRGGGGV RLADAIGTSK AKELVFSGRF VGAEEALEIG LVDEMVAPDH VYEAALAWAR RFSDHPVDVL AAAKASLNGP VNWRGPRPTD RMVDRP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacgagttc atcaagatcg tcctcggttc cgcgcccgag cagacgggcg tggccacgtt gctgttgtcg cggcccccga cgaacgcgtt gacccgccag atgtaccgcg agatctccat cgccgcgaac gagctcgccc agcgcgccga cgtgtcgtcg gtcatcgtct acggcggcca cgagatcttc tgcgcgggcg acgacatccc cgaactgcgc accctcgacg ccgaagagac cgcggcggcc gaccacgcgc tgcagcgctg catcgaggcc gttgccgcca tccccaaacc cacggtcgcg gccgtcaccg ggtacgcgct cggcagcggg atgaacctgg cgctggccgc ggactggcgg gtgagcggcg acaacgccaa gttcggcgcc accgagatcc tggcggggct ggcaccgcgc ggcggcggcg gggtacgcct ggcggacgcc atcgggacca gcaaggccaa ggagctcgtg ttcagcggcc ggttcgtcgg cgccgaggaa gcgctcgaga tcggcctggt cgacgagatg gtggcacccg accacgtcta cgaggccgcg ctcgcgtggg cccgccggtt ctccgaccat ccggtcgacg tgctggccgc cgcgaaagcc tccctcaacg ggccggtcaa ctggcggggc ccccgcccga ccgatcggat ggtggaccgg cccaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MysmA.00358.o.A1.PS00593
PURIFICATION DATe: 5/10/2010
CONCENTRATION: 3.31mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 34
VIAL VOLUME: 15µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNEFIKIVL GSAPEQTGVA TLLLSRPPTN ALTRQMYREI SIAANELAQR ADVSSVIVYG GHEIFCAGDD IPELRTLDAE ETAAADHALQ RCIEAVAAIP KPTVAAVTGY ALGSGMNLAL AADWRVSGDN AKFGATEILA GLAPRGGGGV RLADAIGTSK AKELVFSGRF VGAEEALEIG LVDEMVAPDH VYEAALAWAR RFSDHPVDVL AAAKASLNGP VNWRGPRPTD RMV
Validated NT Sequence
ccaccatccg atcggtcggg cgggggcccc gccagttgac cggcccgttg agggaggctt tcgcggcggc cagcacgtcg accggatggt cggagaaccg gcgggcccac gcgagcgcgg cctcgtagac gtggtcgggt gccaccatct cgtcgaccag gccgatctcg agcgcttcct cggcgccgac gaaccggccg ctgaacacga gctccttggc cttgctggtc ccgatggcgt ccgccaggcg taccccgccg ccgccgcgcg gtgccagccc cgccaggatc tcggtggcgc cgaacttggc gttgtcgccg ctcacccgcc agtccgcggc cagcgccagg ttcatcccgc tgccgagcgc gtacccggtg acggccgcga ccgtgggttt ggggatggcg gcaacggcct cgatgcagcg ctgcagcgcg tggtcggccg ccgcggtctc ttcggcgtcg agggtgcgca gttcggggat gtcgtcgccc gcgcagaaga tctcgtggcc gccgtagacg atgaccgacg acacgtcggc gcgctgggcg agctcgttcg cggcgatgga gatctcgcgg tacatctggc gggtcaacgc gttcgtcggg ggccgcgaca acagcaacgt ggccacgccc gtctgctcgg gcgcggaacc gaggacgatc ttgatgaact cgttcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNEFIKIVL GSAPEQTGVA TLLLSRPPTN ALTRQMYREI SIAANELAQR ADVSSVIVYG GHEIFCAGDD IPELRTLDAE ETAAADHALQ RCIEAVAAIP KPTVAAVTGY ALGSGMNLAL AADWRVSGDN AKFGATEILA GLAPRGGGGV RLADAIGTSK AKELVFSGRF VGAEEALEIG LVDEMVAPDH VYEAALAWAR RFSDHPVDVL AAAKASLNGP VNWRGPRPTD RMVDRP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACGAGTTC ATCAAGATCG TCCTCGGTTC CGCGCCCGAG CAGACGGGCG TGGCCACGTT GCTGTTGTCG CGGCCCCCGA CGAACGCGTT GACCCGCCAG ATGTACCGCG AGATCTCCAT CGCCGCGAAC GAGCTCGCCC AGCGCGCCGA CGTGTCGTCG GTCATCGTCT ACGGCGGCCA CGAGATCTTC TGCGCGGGCG ACGACATCCC CGAACTGCGC ACCCTCGACG CCGAAGAGAC CGCGGCGGCC GACCACGCGC TGCAGCGCTG CATCGAGGCC GTTGCCGCCA TCCCCAAACC CACGGTCGCG GCCGTCACCG GGTACGCGCT CGGCAGCGGG ATGAACCTGG CGCTGGCCGC GGACTGGCGG GTGAGCGGCG ACAACGCCAA GTTCGGCGCC ACCGAGATCC TGGCGGGGCT GGCACCGCGC GGCGGCGGCG GGGTACGCCT GGCGGACGCC ATCGGGACCA GCAAGGCCAA GGAGCTCGTG TTCAGCGGCC GGTTCGTCGG CGCCGAGGAA GCGCTCGAGA TCGGCCTGGT CGACGAGATG GTGGCACCCG ACCACGTCTA CGAGGCCGCG CTCGCGTGGG CCCGCCGGTT CTCCGACCAT CCGGTCGACG TGCTGGCCGC CGCGAAAGCC TCCCTCAACG GGCCGGTCAA CTGGCGGGGC CCCCGCCCGA CCGATCGGAT GGTGGACCGG CCCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA