MysmA.00358.k

Enoyl-CoA hydratase/isomerase family protein

CENTER ID: MysmA.00358.k
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00358.k.A1.GE28622 Full length( MysmA.00358.k ) 1 289
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00358.k.A1.PS00572 Full length( MysmA.00358.k ) 1 289 unavailable

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.2190
RefSeq: YP_886575.1
UniProt: A0QUI7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLFEVDDDHR IATITLNNPK QRNSYDAAMR DQLARYLDIV AEDDDLTVVL LRGAEGVFST GADMNNAYGW YGSKAENGKA EQEQPKSRPS QRRRLTVDRK TFGFYHNFMG FPKVTVGEIS GFALGGGFEM ALMTDISVIA RDTRIGMPAT RFLGPALGSL HMFFHRLGPV LARRLLLTGD VIEAGAIEHL GIFTETCDGS QVAARARYWA QKAAKMPADG VVIAKEAFRL VEQSQAYQGE EVASYLFHAY GTNLQFAPGE FNFVKTRAQH GTKEAFRLRD QHFHVPEPQ
NT Sequence
GTGCTGTTCG AGGTCGACGA CGACCACCGC ATAGCCACCA TCACGCTCAA CAATCCGAAG CAACGCAACT CGTATGACGC CGCGATGCGG GATCAACTGG CACGCTACCT CGACATCGTC GCCGAGGACG ACGATCTGAC CGTGGTGCTG CTGCGCGGGG CCGAAGGCGT GTTCAGTACC GGGGCGGACA TGAACAACGC CTACGGGTGG TATGGCTCGA AGGCTGAGAA CGGCAAAGCC GAACAAGAAC AACCTAAGTC GCGGCCCAGT CAGCGCCGCA GGCTCACCGT CGACCGCAAG ACGTTCGGCT TCTACCACAA CTTCATGGGC TTCCCGAAGG TCACCGTGGG GGAGATCAGC GGTTTCGCGC TGGGCGGCGG TTTCGAGATG GCGCTGATGA CCGACATCTC GGTGATCGCG CGCGACACCC GCATCGGCAT GCCCGCCACA CGCTTCCTGG GGCCTGCGCT GGGCAGTCTG CACATGTTCT TCCACCGATT GGGGCCTGTG CTGGCGCGCC GGCTGCTGCT CACCGGCGAC GTCATCGAGG CAGGCGCGAT CGAGCACCTC GGCATCTTCA CCGAGACGTG CGACGGTTCG CAGGTCGCCG CCCGTGCGCG GTACTGGGCG CAGAAGGCGG CAAAGATGCC CGCTGACGGT GTCGTGATCG CCAAGGAGGC GTTCCGACTG GTTGAGCAGA GTCAGGCGTA TCAGGGCGAA GAGGTCGCCA GCTACCTGTT CCACGCGTAC GGCACCAACC TGCAGTTCGC GCCGGGCGAG TTCAACTTCG TCAAAACCCG CGCCCAGCAC GGCACCAAGG AGGCGTTTCG ACTGCGTGAC CAGCATTTTC ATGTGCCGGA ACCACAG
Details for MysmA.00358.k.A1.GE28622
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 23
PERCENT COVERAGE: 97
Validated AA Sequence
MKMLVTQSKR LLGAVLGAGF DEVELARREL QVGAVRVEQV AGDLFALIRL TLLNQSERLL GDHDTVSGHL CRLLRPVPRT GGDLRTVARL GEDAEVLDRA CLDDVAGEQQ PARQHRPQSV EEHVQTAQRR PQEACGGHAD AGVARDHRDV GHQRHLETAA QRETADLPHG DLREAHEVVV EAERLAVDGE PAALTGPRLR LFLFGFAVLS LRAIPPVGVV HVRPGTEHAF GPAQQHHGQI VVLGDDVEVA CQLIPHRGVI RVALLRIVER DGGYAVVVVD LEQHHRTRTL GLSFQGTHMM VMVM
Validated NT Sequence
ttattactgt ggttccggca catgaaaatg ctggtcacgc agtcgaaacg cctccttggt gccgtgctgg gcgcgggttt tgacgaagtt gaactcgccc ggcgcgaact gcaggttggt gccgtacgcg tggaacaggt agctggcgac ctcttcgccc tgatacgcct gactctgctc aaccagtcgg aacgcctcct tggcgatcac gacaccgtca gcgggcatct ttgccgcctt ctgcgcccag taccgcgcac gggcggcgac ctgcgaaccg tcgcacgtct cggtgaagat gccgaggtgc tcgatcgcgc ctgcctcgat gacgtcgccg gtgagcagca gccggcgcgc cagcacaggc cccaatcggt ggaagaacat gtgcagactg cccagcgcag gccccaggaa gcgtgtggcg ggcatgccga tgcgggtgtc gcgcgcgatc accgagatgt cggtcatcag cgccatctcg aaaccgccgc ccagcgcgaa accgctgatc tcccccacgg tgaccttcgg gaagcccatg aagttgtggt agaagccgaa cgtcttgcgg tcgacggtga gcctgcggcg ctgactgggc cgcgacttag gttgttcttg ttcggctttg ccgttctcag ccttcgagcc ataccacccg taggcgttgt tcatgtccgc cccggtactg aacacgcctt cggccccgcg cagcagcacc acggtcagat cgtcgtcctc ggcgacgatg tcgaggtagc gtgccagttg atcccgcatc gcggcgtcat acgagttgcg ttgcttcgga ttgttgagcg tgatggtggc tatgcggtgg tcgtcgtcga cctcgaacag caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLFEVDDD HRIATITLNN PKQRNSYDAA MRDQLARYLD IVAEDDDLTV VLLRGAEGVF STGADMNNAY GWYGSKAENG KAEQEQPKSR PSQRRRLTVD RKTFGFYHNF MGFPKVTVGE ISGFALGGGF EMALMTDISV IARDTRIGMP ATRFLGPALG SLHMFFHRLG PVLARRLLLT GDVIEAGAIE HLGIFTETCD GSQVAARARY WAQKAAKMPA DGVVIAKEAF RLVEQSQAYQ GEEVASYLFH AYGTNLQFAP GEFNFVKTRA QHGTKEAFRL RDQHFHVPEP Q
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgctgttc gaggtcgacg acgaccaccg catagccacc atcacgctca acaatccgaa gcaacgcaac tcgtatgacg ccgcgatgcg ggatcaactg gcacgctacc tcgacatcgt cgccgaggac gacgatctga ccgtggtgct gctgcgcggg gccgaaggcg tgttcagtac cggggcggac atgaacaacg cctacgggtg gtatggctcg aaggctgaga acggcaaagc cgaacaagaa caacctaagt cgcggcccag tcagcgccgc aggctcaccg tcgaccgcaa gacgttcggc ttctaccaca acttcatggg cttcccgaag gtcaccgtgg gggagatcag cggtttcgcg ctgggcggcg gtttcgagat ggcgctgatg accgacatct cggtgatcgc gcgcgacacc cgcatcggca tgcccgccac acgcttcctg gggcctgcgc tgggcagtct gcacatgttc ttccaccgat tggggcctgt gctggcgcgc cggctgctgc tcaccggcga cgtcatcgag gcaggcgcga tcgagcacct cggcatcttc accgagacgt gcgacggttc gcaggtcgcc gcccgtgcgc ggtactgggc gcagaaggcg gcaaagatgc ccgctgacgg tgtcgtgatc gccaaggagg cgttccgact ggttgagcag agtcaggcgt atcagggcga agaggtcgcc agctacctgt tccacgcgta cggcaccaac ctgcagttcg cgccgggcga gttcaacttc gtcaaaaccc gcgcccagca cggcaccaag gaggcgtttc gactgcgtga ccagcatttt catgtgccgg aaccacagaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MysmA.00358.k.A1.PS00572
PURIFICATION DATe: 4/22/2010
CONCENTRATION: 37.01mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 23
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MKMLVTQSKR LLGAVLGAGF DEVELARREL QVGAVRVEQV AGDLFALIRL TLLNQSERLL GDHDTVSGHL CRLLRPVPRT GGDLRTVARL GEDAEVLDRA CLDDVAGEQQ PARQHRPQSV EEHVQTAQRR PQEACGGHAD AGVARDHRDV GHQRHLETAA QRETADLPHG DLREAHEVVV EAERLAVDGE PAALTGPRLR LFLFGFAVLS LRAIPPVGVV HVRPGTEHAF GPAQQHHGQI VVLGDDVEVA CQLIPHRGVI RVALLRIVER DGGYAVVVVD LEQHHRTRTL GLSFQGTHMM VMVM
Validated NT Sequence
ttattactgt ggttccggca catgaaaatg ctggtcacgc agtcgaaacg cctccttggt gccgtgctgg gcgcgggttt tgacgaagtt gaactcgccc ggcgcgaact gcaggttggt gccgtacgcg tggaacaggt agctggcgac ctcttcgccc tgatacgcct gactctgctc aaccagtcgg aacgcctcct tggcgatcac gacaccgtca gcgggcatct ttgccgcctt ctgcgcccag taccgcgcac gggcggcgac ctgcgaaccg tcgcacgtct cggtgaagat gccgaggtgc tcgatcgcgc ctgcctcgat gacgtcgccg gtgagcagca gccggcgcgc cagcacaggc cccaatcggt ggaagaacat gtgcagactg cccagcgcag gccccaggaa gcgtgtggcg ggcatgccga tgcgggtgtc gcgcgcgatc accgagatgt cggtcatcag cgccatctcg aaaccgccgc ccagcgcgaa accgctgatc tcccccacgg tgaccttcgg gaagcccatg aagttgtggt agaagccgaa cgtcttgcgg tcgacggtga gcctgcggcg ctgactgggc cgcgacttag gttgttcttg ttcggctttg ccgttctcag ccttcgagcc ataccacccg taggcgttgt tcatgtccgc cccggtactg aacacgcctt cggccccgcg cagcagcacc acggtcagat cgtcgtcctc ggcgacgatg tcgaggtagc gtgccagttg atcccgcatc gcggcgtcat acgagttgcg ttgcttcgga ttgttgagcg tgatggtggc tatgcggtgg tcgtcgtcga cctcgaacag caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLFEVDDD HRIATITLNN PKQRNSYDAA MRDQLARYLD IVAEDDDLTV VLLRGAEGVF STGADMNNAY GWYGSKAENG KAEQEQPKSR PSQRRRLTVD RKTFGFYHNF MGFPKVTVGE ISGFALGGGF EMALMTDISV IARDTRIGMP ATRFLGPALG SLHMFFHRLG PVLARRLLLT GDVIEAGAIE HLGIFTETCD GSQVAARARY WAQKAAKMPA DGVVIAKEAF RLVEQSQAYQ GEEVASYLFH AYGTNLQFAP GEFNFVKTRA QHGTKEAFRL RDQHFHVPEP Q
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCTGTTC GAGGTCGACG ACGACCACCG CATAGCCACC ATCACGCTCA ACAATCCGAA GCAACGCAAC TCGTATGACG CCGCGATGCG GGATCAACTG GCACGCTACC TCGACATCGT CGCCGAGGAC GACGATCTGA CCGTGGTGCT GCTGCGCGGG GCCGAAGGCG TGTTCAGTAC CGGGGCGGAC ATGAACAACG CCTACGGGTG GTATGGCTCG AAGGCTGAGA ACGGCAAAGC CGAACAAGAA CAACCTAAGT CGCGGCCCAG TCAGCGCCGC AGGCTCACCG TCGACCGCAA GACGTTCGGC TTCTACCACA ACTTCATGGG CTTCCCGAAG GTCACCGTGG GGGAGATCAG CGGTTTCGCG CTGGGCGGCG GTTTCGAGAT GGCGCTGATG ACCGACATCT CGGTGATCGC GCGCGACACC CGCATCGGCA TGCCCGCCAC ACGCTTCCTG GGGCCTGCGC TGGGCAGTCT GCACATGTTC TTCCACCGAT TGGGGCCTGT GCTGGCGCGC CGGCTGCTGC TCACCGGCGA CGTCATCGAG GCAGGCGCGA TCGAGCACCT CGGCATCTTC ACCGAGACGT GCGACGGTTC GCAGGTCGCC GCCCGTGCGC GGTACTGGGC GCAGAAGGCG GCAAAGATGC CCGCTGACGG TGTCGTGATC GCCAAGGAGG CGTTCCGACT GGTTGAGCAG AGTCAGGCGT ATCAGGGCGA AGAGGTCGCC AGCTACCTGT TCCACGCGTA CGGCACCAAC CTGCAGTTCG CGCCGGGCGA GTTCAACTTC GTCAAAACCC GCGCCCAGCA CGGCACCAAG GAGGCGTTTC GACTGCGTGA CCAGCATTTT CATGTGCCGG AACCACAGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA