MysmA.00358.e
Probable enoyl-CoA hydratase
| CENTER ID: | MysmA.00358.e |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00358.e.A1.GE28616 | Full length( MysmA.00358.e ) | 1 | 260 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00358.e.A1.PS00544 | Full length( MysmA.00358.e ) | 1 | 260 |
Structures
3OME
DEPOSITED: 8/26/2010
DETERMINATION: XRay
CLONE: MysmA.00358.e.A1.GE28616
PROTEIN: MysmA.00358.e.A1.PS00544
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | NO_GROUP |
| PATRIC ID: | fig|246196.19.peg.4729 |
| RefSeq: | YP_889102.1 |
| UniProt: | A0R1R4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYIDYGVADS IATITLNRPE AANAQNPELL DELDAAWTRA AEDNEVKVII LRANGKHFSA GHDLRGGGEV PEKISLEFII QHEARRYLDY TLRWRNVPKP SIAAVQGRCI SGGLLLCWPC DLILASDDAL FSDPVALMGI GGVEYHGHTW ELGPRKAKEI LFTGRALTAE EAERTGMVNR VVARDELDAQ TRELAEQIAT MPPFALRQAK RAVNQTLDVQ GFYAAIQSVF DIHQTGHGNA LSVSGWPVLV DIEEMKANIK
NT Sequence
GTGTACATCG ACTACGGCGT CGCCGACTCG ATCGCGACCA TCACGCTCAA CCGCCCCGAG GCCGCCAACG CGCAGAACCC CGAACTGCTC GACGAACTCG ACGCGGCCTG GACCAGGGCC GCCGAGGACA ACGAGGTCAA GGTCATCATT CTGCGGGCCA ACGGAAAGCA CTTCTCCGCC GGGCACGATC TGCGTGGCGG TGGTGAAGTG CCCGAGAAGA TCTCACTGGA ATTCATCATC CAGCACGAGG CCAGGCGCTA TCTCGACTAC ACGCTGCGCT GGCGCAACGT GCCCAAGCCG TCGATCGCGG CCGTCCAGGG GCGCTGCATC TCGGGTGGTC TGCTGCTGTG CTGGCCGTGT GATCTGATCC TGGCGTCCGA CGACGCGCTG TTCTCCGACC CGGTGGCGCT CATGGGCATC GGTGGCGTCG AATACCACGG TCACACGTGG GAACTCGGCC CGCGCAAGGC CAAGGAGATT TTGTTCACCG GGCGCGCGCT GACGGCGGAG GAGGCCGAGC GCACCGGCAT GGTCAACCGC GTCGTCGCGC GTGACGAACT CGACGCGCAG ACCCGCGAGC TGGCCGAACA GATCGCCACG ATGCCGCCGT TCGCACTGCG CCAGGCCAAG CGGGCCGTGA ACCAGACACT CGACGTGCAG GGCTTCTACG CGGCCATCCA ATCGGTCTTC GACATCCACC AGACCGGGCA CGGCAACGCG CTGAGCGTCA GCGGTTGGCC GGTGCTGGTC GACATCGAGG AAATGAAGGC CAACATCAAG
Details for MysmA.00358.e.A1.GE28616
| HARVESTED ON: | 2/17/2010 |
| SEQUENCED ON: | 2/24/2010 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVYIDYGVA DSIATITLNR PEAANAQNPE LLDELDAAWT RAAEDNEVKV IILRANGKHF SAGHDLRGGG EVPEKISLEF IIQHEARRYL DYTLRWRNVP KPSIAAVQGR CISGGLLLCW PCDLILASDD ALFSDPVALM GIGGVEYHGH TWELGPRKAK EILFTGRALT AEEAERTGMV NRVVARDELD AQTRELAEQI ATMPPFALRQ AKRAVNQTLD VQGFYAAIQS VFDIHQTGHG NALSVSGWPV LVDIEEMKAN IK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt acatcgacta cggcgtcgcc gactcgatcg cgaccatcac gctcaaccgc cccgaggccg ccaacgcgca gaaccccgaa ctgctcgacg aactcgacgc ggcctggacc agggccgccg aggacaacga ggtcaaggtc atcattctgc gggccaacgg aaagcacttc tccgccgggc acgatctgcg tggcggtggt gaagtgcccg agaagatctc actggaattc atcatccagc acgaggccag gcgctatctc gactacacgc tgcgctggcg caacgtgccc aagccgtcga tcgcggccgt ccaggggcgc tgcatctcgg gtggtctgct gctgtgctgg ccgtgtgatc tgatcctggc gtccgacgac gcgctgttct ccgacccggt ggcgctcatg ggcatcggtg gcgtcgaata ccacggtcac acgtgggaac tcggcccgcg caaggccaag gagattttgt tcaccgggcg cgcgctgacg gcggaggagg ccgagcgcac cggcatggtc aaccgcgtcg tcgcgcgtga cgaactcgac gcgcagaccc gcgagctggc cgaacagatc gccacgatgc cgccgttcgc actgcgccag gccaagcggg ccgtgaacca gacactcgac gtgcagggct tctacgcggc catccaatcg gtcttcgaca tccaccagac cgggcacggc aacgcgctga gcgtcagcgg ttggccggtg ctggtcgaca tcgaggaaat gaaggccaac atcaagtaat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVYIDYGVA DSIATITLNR PEAANAQNPE LLDELDAAWT RAAEDNEVKV IILRANGKHF SAGHDLRGGG EVPEKISLEF IIQHEARRYL DYTLRWRNVP KPSIAAVQGR CISGGLLLCW PCDLILASDD ALFSDPVALM GIGGVEYHGH TWELGPRKAK EILFTGRALT AEEAERTGMV NRVVARDELD AQTRELAEQI ATMPPFALRQ AKRAVNQTLD VQGFYAAIQS VFDIHQTGHG NALSVSGWPV LVDIEEMKAN IK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtacatc gactacggcg tcgccgactc gatcgcgacc atcacgctca accgccccga ggccgccaac gcgcagaacc ccgaactgct cgacgaactc gacgcggcct ggaccagggc cgccgaggac aacgaggtca aggtcatcat tctgcgggcc aacggaaagc acttctccgc cgggcacgat ctgcgtggcg gtggtgaagt gcccgagaag atctcactgg aattcatcat ccagcacgag gccaggcgct atctcgacta cacgctgcgc tggcgcaacg tgcccaagcc gtcgatcgcg gccgtccagg ggcgctgcat ctcgggtggt ctgctgctgt gctggccgtg tgatctgatc ctggcgtccg acgacgcgct gttctccgac ccggtggcgc tcatgggcat cggtggcgtc gaataccacg gtcacacgtg ggaactcggc ccgcgcaagg ccaaggagat tttgttcacc gggcgcgcgc tgacggcgga ggaggccgag cgcaccggca tggtcaaccg cgtcgtcgcg cgtgacgaac tcgacgcgca gacccgcgag ctggccgaac agatcgccac gatgccgccg ttcgcactgc gccaggccaa gcgggccgtg aaccagacac tcgacgtgca gggcttctac gcggccatcc aatcggtctt cgacatccac cagaccgggc acggcaacgc gctgagcgtc agcggttggc cggtgctggt cgacatcgag gaaatgaagg ccaacatcaa gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MysmA.00358.e.A1.PS00544
| PURIFICATION DATe: | 4/2/2010 |
| CONCENTRATION: | 61.16mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVYIDYGVA DSIATITLNR PEAANAQNPE LLDELDAAWT RAAEDNEVKV IILRANGKHF SAGHDLRGGG EVPEKISLEF IIQHEARRYL DYTLRWRNVP KPSIAAVQGR CISGGLLLCW PCDLILASDD ALFSDPVALM GIGGVEYHGH TWELGPRKAK EILFTGRALT AEEAERTGMV NRVVARDELD AQTRELAEQI ATMPPFALRQ AKRAVNQTLD VQGFYAAIQS VFDIHQTGHG NALSVSGWPV LVDIEEMKAN IK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt acatcgacta cggcgtcgcc gactcgatcg cgaccatcac gctcaaccgc cccgaggccg ccaacgcgca gaaccccgaa ctgctcgacg aactcgacgc ggcctggacc agggccgccg aggacaacga ggtcaaggtc atcattctgc gggccaacgg aaagcacttc tccgccgggc acgatctgcg tggcggtggt gaagtgcccg agaagatctc actggaattc atcatccagc acgaggccag gcgctatctc gactacacgc tgcgctggcg caacgtgccc aagccgtcga tcgcggccgt ccaggggcgc tgcatctcgg gtggtctgct gctgtgctgg ccgtgtgatc tgatcctggc gtccgacgac gcgctgttct ccgacccggt ggcgctcatg ggcatcggtg gcgtcgaata ccacggtcac acgtgggaac tcggcccgcg caaggccaag gagattttgt tcaccgggcg cgcgctgacg gcggaggagg ccgagcgcac cggcatggtc aaccgcgtcg tcgcgcgtga cgaactcgac gcgcagaccc gcgagctggc cgaacagatc gccacgatgc cgccgttcgc actgcgccag gccaagcggg ccgtgaacca gacactcgac gtgcagggct tctacgcggc catccaatcg gtcttcgaca tccaccagac cgggcacggc aacgcgctga gcgtcagcgg ttggccggtg ctggtcgaca tcgaggaaat gaaggccaac atcaagtaat aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVYIDYGVA DSIATITLNR PEAANAQNPE LLDELDAAWT RAAEDNEVKV IILRANGKHF SAGHDLRGGG EVPEKISLEF IIQHEARRYL DYTLRWRNVP KPSIAAVQGR CISGGLLLCW PCDLILASDD ALFSDPVALM GIGGVEYHGH TWELGPRKAK EILFTGRALT AEEAERTGMV NRVVARDELD AQTRELAEQI ATMPPFALRQ AKRAVNQTLD VQGFYAAIQS VFDIHQTGHG NALSVSGWPV LVDIEEMKAN IK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTACATC GACTACGGCG TCGCCGACTC GATCGCGACC ATCACGCTCA ACCGCCCCGA GGCCGCCAAC GCGCAGAACC CCGAACTGCT CGACGAACTC GACGCGGCCT GGACCAGGGC CGCCGAGGAC AACGAGGTCA AGGTCATCAT TCTGCGGGCC AACGGAAAGC ACTTCTCCGC CGGGCACGAT CTGCGTGGCG GTGGTGAAGT GCCCGAGAAG ATCTCACTGG AATTCATCAT CCAGCACGAG GCCAGGCGCT ATCTCGACTA CACGCTGCGC TGGCGCAACG TGCCCAAGCC GTCGATCGCG GCCGTCCAGG GGCGCTGCAT CTCGGGTGGT CTGCTGCTGT GCTGGCCGTG TGATCTGATC CTGGCGTCCG ACGACGCGCT GTTCTCCGAC CCGGTGGCGC TCATGGGCAT CGGTGGCGTC GAATACCACG GTCACACGTG GGAACTCGGC CCGCGCAAGG CCAAGGAGAT TTTGTTCACC GGGCGCGCGC TGACGGCGGA GGAGGCCGAG CGCACCGGCA TGGTCAACCG CGTCGTCGCG CGTGACGAAC TCGACGCGCA GACCCGCGAG CTGGCCGAAC AGATCGCCAC GATGCCGCCG TTCGCACTGC GCCAGGCCAA GCGGGCCGTG AACCAGACAC TCGACGTGCA GGGCTTCTAC GCGGCCATCC AATCGGTCTT CGACATCCAC CAGACCGGGC ACGGCAACGC GCTGAGCGTC AGCGGTTGGC CGGTGCTGGT CGACATCGAG GAAATGAAGG CCAACATCAA GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA