MysmA.00339.a

Alanine racemase

CENTER ID: MysmA.00339.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00339.a.B1.GE40210 Full length(MysmA.00339.a) 1 389
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00339.a.B1.PW37969 Full length(MysmA.00339.a) 1 389

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.1561
UniProt: A0QSR6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQTTEPMTPP APLASAQTVI DLGAIDHNVR VLRELAGSAD VMAVVKADAY GHGALPVART ALAAGAAALG VATIPEALAL REGGITAPVL AWLHPPGTDF APAIAADVEV AVSSRRQLEQ VTAAAAEVGR TATVTVKVDT GLSRNGVGAA DYPEVLDVLR RAQADGAIRV RGLMSHLVHG DDPENPFNGL QGQRLADMRV YAREHGVDYE VAHLCNSPAA MTRPDLAFEM VRPGISLYGL SPIPERGDMG LRPAMTLKCP VALVRSVHAG DGVSYGHRWV ADRDTTLGLL PIGYADGVYR ALSGRIDVLI KGRRRRAVGR ICMDQFVVDL GPDADDVAVG DDAILFGPGA NGEPTAQDWA ELLDTIHYEV VTSPRGRVTR TYLPAGQQD
NT Sequence
ATGCAGACCA CCGAGCCCAT GACACCGCCT GCGCCACTGG CGTCGGCACA GACGGTGATC GATCTGGGCG CCATCGACCA CAACGTGCGC GTGCTGCGCG AACTCGCCGG TTCCGCGGAC GTGATGGCGG TGGTCAAGGC CGACGCCTAC GGACACGGGG CGCTCCCGGT GGCCCGCACC GCGCTGGCCG CCGGGGCCGC CGCCCTCGGT GTCGCGACCA TCCCCGAGGC GCTTGCGCTG CGTGAGGGTG GCATCACGGC GCCGGTCCTG GCGTGGCTGC ATCCGCCCGG CACCGACTTC GCCCCGGCGA TCGCCGCCGA CGTCGAGGTC GCCGTGTCGT CGCGCCGCCA ACTCGAACAG GTGACGGCGG CCGCGGCCGA GGTGGGCCGC ACCGCGACGG TGACCGTCAA GGTCGACACC GGGCTCAGCC GCAACGGCGT CGGCGCGGCC GACTATCCCG AGGTGCTCGA TGTCCTGCGC CGCGCGCAGG CCGACGGGGC GATCCGGGTG CGCGGTCTGA TGAGCCACCT GGTGCACGGC GACGACCCGG AGAACCCGTT CAACGGTCTG CAGGGCCAAC GGCTGGCGGA CATGCGTGTT TACGCGCGCG AGCACGGCGT CGACTACGAG GTGGCGCACC TGTGCAACTC GCCTGCGGCC ATGACCAGGC CCGACCTGGC GTTCGAGATG GTGCGTCCCG GCATCTCGCT GTACGGGCTC AGTCCCATCC CTGAGCGCGG CGACATGGGC CTGCGGCCCG CGATGACCTT GAAATGCCCG GTGGCGCTTG TCCGTTCGGT GCACGCCGGG GACGGGGTGT CGTACGGGCA CCGGTGGGTG GCCGACCGTG ACACCACCCT GGGGCTGCTG CCGATCGGCT ACGCCGACGG CGTGTACCGC GCACTGAGCG GGCGTATCGA CGTGCTGATC AAGGGCAGGC GCAGGCGCGC CGTCGGCCGG ATCTGCATGG ACCAGTTCGT GGTCGACCTC GGCCCGGACG CGGACGACGT GGCCGTAGGT GACGACGCCA TCCTGTTCGG CCCGGGCGCC AACGGCGAAC CGACCGCGCA GGACTGGGCC GAACTGCTCG ACACCATTCA CTACGAGGTC GTCACGAGCC CGCGCGGACG CGTCACGCGC ACGTATCTTC CGGCAGGACA ACAAGAT
Details for MysmA.00339.a.B1.GE40210
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/24/2016
EXPECTED MW: 42kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMQ TTEPMTPPAP LASAQTVIDL GAIDHNVRVL RELAGSADVM AVVKADAYGH GALPVARTAL AAGAAALGVA TIPEALALRE GGITAPVLAW LHPPGTDFAP AIAADVEVAV SSRRQLEQVT AAAAEVGRTA TVTVKVDTGL SRNGVGAADY PEVLDVLRRA QADGAIRVRG LMSHLVHGDD PENPFNGLQG QRLADMRVYA REHGVDYEVA HLCNSPAAMT RPDLAFEMVR PGISLYGLSP IPERGDMGLR PAMTLKCPVA LVRSVHAGDG VSYGHRWVAD RDTTLGLLPI GYADGVYRAL SGRIDVLIKG RRRRAVGRIC MDQFVVDLGP DADDVAVGDD AILFGPGANG EPTAQDWAEL LDTIHYEVVT SPRGRVTR
Validated NT Sequence
atggctcacc accaccacca ccatatgcag accaccgagc ccatgacacc gcctgcgcca ctggcgtcgg cacagacggt gatcgatctg ggcgccatcg accacaacgt gcgcgtgctg cgcgaactcg ccggttccgc ggacgtgatg gcggtggtca aggccgacgc ctacggacac ggggcgctcc cggtggcccg caccgcgctg gccgccgggg ccgccgccct cggtgtcgcg accatccccg aggcgcttgc gctgcgtgag ggtggcatca cggcgccggt cctggcgtgg ctgcatccgc ccggcaccga cttcgccccg gcgatcgccg ccgacgtcga ggtcgccgtg tcgtcgcgcc gccaactcga acaggtgacg gcggccgcgg ccgaggtggg ccgcaccgcg acggtgaccg tcaaggtcga caccgggctc agccgcaacg gcgtcggcgc ggccgactat cccgaggtgc tcgatgtcct gcgccgcgcg caggccgacg gggcgatccg ggtgcgcggt ctgatgagcc acctggtgca cggcgacgac ccggagaacc cgttcaacgg tctgcagggc caacggctgg cggacatgcg tgtttacgcg cgcgagcacg gcgtcgacta cgaggtggcg cacctgtgca actcgcctgc ggccatgacc aggcccgacc tggcgttcga gatggtgcgt cccggcatct cgctgtacgg gctcagtccc atccctgagc gcggcgacat gggcctgcgg cccgcgatga ccttgaaatg cccggtggcg cttgtccgtt cggtgcacgc cggggacggg gtgtcgtacg ggcaccggtg ggtggccgac cgtgacacca ccctggggct gctgccgatc ggctacgccg acggcgtgta ccgcgcactg agcgggcgta tcgacgtgct gatcaagggc aggcgcaggc gcgccgtcgg ccggatctgc atggaccagt tcgtggtcga cctcggcccg gacgcggacg acgtggccgt aggtgacgac gccatcctgt tcggcccggg cgccaacggc gaaccgaccg cgcaggactg ggccgaactg ctcgacacca ttcactacga ggtcgtcacg agcccgcgcg gacgcgtcac gcgca
Expected Protein Sequence
MAHHHHHHMQ TTEPMTPPAP LASAQTVIDL GAIDHNVRVL RELAGSADVM AVVKADAYGH GALPVARTAL AAGAAALGVA TIPEALALRE GGITAPVLAW LHPPGTDFAP AIAADVEVAV SSRRQLEQVT AAAAEVGRTA TVTVKVDTGL SRNGVGAADY PEVLDVLRRA QADGAIRVRG LMSHLVHGDD PENPFNGLQG QRLADMRVYA REHGVDYEVA HLCNSPAAMT RPDLAFEMVR PGISLYGLSP IPERGDMGLR PAMTLKCPVA LVRSVHAGDG VSYGHRWVAD RDTTLGLLPI GYADGVYRAL SGRIDVLIKG RRRRAVGRIC MDQFVVDLGP DADDVAVGDD AILFGPGANG EPTAQDWAEL LDTIHYEVVT SPRGRVTRTY LPAGQQD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaga ccaccgagcc catgacaccg cctgcgccac tggcgtcggc acagacggtg atcgatctgg gcgccatcga ccacaacgtg cgcgtgctgc gcgaactcgc cggttccgcg gacgtgatgg cggtggtcaa ggccgacgcc tacggacacg gggcgctccc ggtggcccgc accgcgctgg ccgccggggc cgccgccctc ggtgtcgcga ccatccccga ggcgcttgcg ctgcgtgagg gtggcatcac ggcgccggtc ctggcgtggc tgcatccgcc cggcaccgac ttcgccccgg cgatcgccgc cgacgtcgag gtcgccgtgt cgtcgcgccg ccaactcgaa caggtgacgg cggccgcggc cgaggtgggc cgcaccgcga cggtgaccgt caaggtcgac accgggctca gccgcaacgg cgtcggcgcg gccgactatc ccgaggtgct cgatgtcctg cgccgcgcgc aggccgacgg ggcgatccgg gtgcgcggtc tgatgagcca cctggtgcac ggcgacgacc cggagaaccc gttcaacggt ctgcagggcc aacggctggc ggacatgcgt gtttacgcgc gcgagcacgg cgtcgactac gaggtggcgc acctgtgcaa ctcgcctgcg gccatgacca ggcccgacct ggcgttcgag atggtgcgtc ccggcatctc gctgtacggg ctcagtccca tccctgagcg cggcgacatg ggcctgcggc ccgcgatgac cttgaaatgc ccggtggcgc ttgtccgttc ggtgcacgcc ggggacgggg tgtcgtacgg gcaccggtgg gtggccgacc gtgacaccac cctggggctg ctgccgatcg gctacgccga cggcgtgtac cgcgcactga gcgggcgtat cgacgtgctg atcaagggca ggcgcaggcg cgccgtcggc cggatctgca tggaccagtt cgtggtcgac ctcggcccgg acgcggacga cgtggccgta ggtgacgacg ccatcctgtt cggcccgggc gccaacggcg aaccgaccgc gcaggactgg gccgaactgc tcgacaccat tcactacgag gtcgtcacga gcccgcgcgg acgcgtcacg cgcacgtatc ttccggcagg acaacaagat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for MysmA.00339.a.B1.PW37969
PURIFICATION DATe: 11/14/2016
CONCENTRATION: 3mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ TTEPMTPPAP LASAQTVIDL GAIDHNVRVL RELAGSADVM AVVKADAYGH GALPVARTAL AAGAAALGVA TIPEALALRE GGITAPVLAW LHPPGTDFAP AIAADVEVAV SSRRQLEQVT AAAAEVGRTA TVTVKVDTGL SRNGVGAADY PEVLDVLRRA QADGAIRVRG LMSHLVHGDD PENPFNGLQG QRLADMRVYA REHGVDYEVA HLCNSPAAMT RPDLAFEMVR PGISLYGLSP IPERGDMGLR PAMTLKCPVA LVRSVHAGDG VSYGHRWVAD RDTTLGLLPI GYADGVYRAL SGRIDVLIKG RRRRAVGRIC MDQFVVDLGP DADDVAVGDD AILFGPGANG EPTAQDWAEL LDTIHYEVVT SPRGRVTR
Validated NT Sequence
atggctcacc accaccacca ccatatgcag accaccgagc ccatgacacc gcctgcgcca ctggcgtcgg cacagacggt gatcgatctg ggcgccatcg accacaacgt gcgcgtgctg cgcgaactcg ccggttccgc ggacgtgatg gcggtggtca aggccgacgc ctacggacac ggggcgctcc cggtggcccg caccgcgctg gccgccgggg ccgccgccct cggtgtcgcg accatccccg aggcgcttgc gctgcgtgag ggtggcatca cggcgccggt cctggcgtgg ctgcatccgc ccggcaccga cttcgccccg gcgatcgccg ccgacgtcga ggtcgccgtg tcgtcgcgcc gccaactcga acaggtgacg gcggccgcgg ccgaggtggg ccgcaccgcg acggtgaccg tcaaggtcga caccgggctc agccgcaacg gcgtcggcgc ggccgactat cccgaggtgc tcgatgtcct gcgccgcgcg caggccgacg gggcgatccg ggtgcgcggt ctgatgagcc acctggtgca cggcgacgac ccggagaacc cgttcaacgg tctgcagggc caacggctgg cggacatgcg tgtttacgcg cgcgagcacg gcgtcgacta cgaggtggcg cacctgtgca actcgcctgc ggccatgacc aggcccgacc tggcgttcga gatggtgcgt cccggcatct cgctgtacgg gctcagtccc atccctgagc gcggcgacat gggcctgcgg cccgcgatga ccttgaaatg cccggtggcg cttgtccgtt cggtgcacgc cggggacggg gtgtcgtacg ggcaccggtg ggtggccgac cgtgacacca ccctggggct gctgccgatc ggctacgccg acggcgtgta ccgcgcactg agcgggcgta tcgacgtgct gatcaagggc aggcgcaggc gcgccgtcgg ccggatctgc atggaccagt tcgtggtcga cctcggcccg gacgcggacg acgtggccgt aggtgacgac gccatcctgt tcggcccggg cgccaacggc gaaccgaccg cgcaggactg ggccgaactg ctcgacacca ttcactacga ggtcgtcacg agcccgcgcg gacgcgtcac gcgca
Expressed Protein Sequence
MAHHHHHHMQ TTEPMTPPAP LASAQTVIDL GAIDHNVRVL RELAGSADVM AVVKADAYGH GALPVARTAL AAGAAALGVA TIPEALALRE GGITAPVLAW LHPPGTDFAP AIAADVEVAV SSRRQLEQVT AAAAEVGRTA TVTVKVDTGL SRNGVGAADY PEVLDVLRRA QADGAIRVRG LMSHLVHGDD PENPFNGLQG QRLADMRVYA REHGVDYEVA HLCNSPAAMT RPDLAFEMVR PGISLYGLSP IPERGDMGLR PAMTLKCPVA LVRSVHAGDG VSYGHRWVAD RDTTLGLLPI GYADGVYRAL SGRIDVLIKG RRRRAVGRIC MDQFVVDLGP DADDVAVGDD AILFGPGANG EPTAQDWAEL LDTIHYEVVT SPRGRVTRTY LPAGQQD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAGA CCACCGAGCC CATGACACCG CCTGCGCCAC TGGCGTCGGC ACAGACGGTG ATCGATCTGG GCGCCATCGA CCACAACGTG CGCGTGCTGC GCGAACTCGC CGGTTCCGCG GACGTGATGG CGGTGGTCAA GGCCGACGCC TACGGACACG GGGCGCTCCC GGTGGCCCGC ACCGCGCTGG CCGCCGGGGC CGCCGCCCTC GGTGTCGCGA CCATCCCCGA GGCGCTTGCG CTGCGTGAGG GTGGCATCAC GGCGCCGGTC CTGGCGTGGC TGCATCCGCC CGGCACCGAC TTCGCCCCGG CGATCGCCGC CGACGTCGAG GTCGCCGTGT CGTCGCGCCG CCAACTCGAA CAGGTGACGG CGGCCGCGGC CGAGGTGGGC CGCACCGCGA CGGTGACCGT CAAGGTCGAC ACCGGGCTCA GCCGCAACGG CGTCGGCGCG GCCGACTATC CCGAGGTGCT CGATGTCCTG CGCCGCGCGC AGGCCGACGG GGCGATCCGG GTGCGCGGTC TGATGAGCCA CCTGGTGCAC GGCGACGACC CGGAGAACCC GTTCAACGGT CTGCAGGGCC AACGGCTGGC GGACATGCGT GTTTACGCGC GCGAGCACGG CGTCGACTAC GAGGTGGCGC ACCTGTGCAA CTCGCCTGCG GCCATGACCA GGCCCGACCT GGCGTTCGAG ATGGTGCGTC CCGGCATCTC GCTGTACGGG CTCAGTCCCA TCCCTGAGCG CGGCGACATG GGCCTGCGGC CCGCGATGAC CTTGAAATGC CCGGTGGCGC TTGTCCGTTC GGTGCACGCC GGGGACGGGG TGTCGTACGG GCACCGGTGG GTGGCCGACC GTGACACCAC CCTGGGGCTG CTGCCGATCG GCTACGCCGA CGGCGTGTAC CGCGCACTGA GCGGGCGTAT CGACGTGCTG ATCAAGGGCA GGCGCAGGCG CGCCGTCGGC CGGATCTGCA TGGACCAGTT CGTGGTCGAC CTCGGCCCGG ACGCGGACGA CGTGGCCGTA GGTGACGACG CCATCCTGTT CGGCCCGGGC GCCAACGGCG AACCGACCGC GCAGGACTGG GCCGAACTGC TCGACACCAT TCACTACGAG GTCGTCACGA GCCCGCGCGG ACGCGTCACG CGCACGTATC TTCCGGCAGG ACAACAAGAT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT