MysmA.00337.b

Short chain dehydrogenase

CENTER ID: MysmA.00337.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00337.b.A1.GE28610 Full length( MysmA.00337.b ) 1 314
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.2180
RefSeq: YP_886565.1
UniProt: A0QUH7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPRKPIDVTL PDLSGRRAVV TGASDGIGLG IAARLAAAGA DVILPVRNEI KGKAAIAAVR QTNAAAKVSL RELDLSSLAS VAALGKSLLE EAQPIHLLIN NAGVMTPPER RTTTDGFESQ FGTNHLGHFA LVAHLLPLLR AGGARVTSQV SVAANQHAIN WDDLNWERSY DGAKAYSQSK IAFGLFGLEL DRRSHAHGWG ITSNLSHPGL APTNLLAARP DTGRAQDDWK LRLIRTLSSR GILFGTVHTA QLPALYAAAS PHAKRGGFYG PRGPGHLGGP PGEQRLYSRL RNNDEAQRIW EISEKLTDMS FPTA
NT Sequence
ATGCCACGTA AACCCATCGA CGTCACGCTT CCCGATCTGT CCGGCAGACG CGCCGTGGTC ACCGGTGCCA GCGACGGCAT CGGACTCGGG ATCGCCGCGC GGCTCGCCGC GGCGGGCGCT GACGTGATCC TGCCCGTACG CAACGAGATC AAGGGAAAGG CGGCCATCGC GGCTGTCCGT CAGACGAACG CCGCGGCGAA AGTGTCGCTG CGTGAGCTCG ACCTGTCGTC CCTGGCCTCC GTTGCGGCAC TCGGCAAGAG CCTTCTCGAG GAGGCACAGC CCATCCATCT GCTCATCAAC AACGCCGGTG TCATGACGCC GCCCGAGCGG CGGACGACCA CCGACGGGTT CGAGTCGCAG TTCGGCACCA ACCACCTCGG CCACTTCGCC CTCGTGGCGC ATCTGCTGCC CCTGCTGCGT GCCGGTGGAG CGCGGGTGAC CTCACAGGTC AGCGTCGCGG CGAATCAGCA CGCCATCAAC TGGGACGATT TGAACTGGGA GCGTTCGTAC GACGGCGCAA AGGCGTACAG CCAGTCGAAG ATCGCGTTCG GGCTCTTCGG CCTGGAACTG GACCGGCGCA GTCACGCCCA CGGGTGGGGC ATCACGAGCA ATCTGTCCCA TCCGGGTCTG GCGCCGACCA ACCTGCTTGC GGCCCGCCCG GACACCGGAC GCGCACAGGA CGACTGGAAA CTGCGCCTGA TCCGTACGCT GTCGTCGCGT GGCATCCTTT TCGGGACCGT GCACACCGCG CAGCTGCCTG CCCTCTACGC CGCGGCCTCG CCCCATGCGA AGCGCGGTGG GTTCTACGGG CCGCGTGGTC CCGGACATCT CGGTGGTCCG CCGGGAGAAC AGAGGCTCTA CTCGCGGCTC CGTAACAACG ATGAGGCTCA ACGTATCTGG GAGATCTCGG AGAAGTTGAC GGACATGTCG TTCCCCACGG CA
Details for MysmA.00337.b.A1.GE28610
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMPRKPID VTLPDLSGRR AVVTGASDGI GLGIAARLAA AGADVILPVR NEIKGKAAIA AVRQTNAAAK VSLRELDLSS LASVAALGKS LLEEAQPIHL LINNAGVMTP PERRTTTDGF ESQFGTNHLG HFALVAHLLP LLRAGGARVT SQVSVAANQH AINWDDLNWE RSYDGAKAYS QSKIAFGLFG LELDRRSHAH GWGITSNLSH PGLAPTNLLA ARPDTGRAQD DWKLRLIRTL SSRGILFGTV HTAQLPALYA AASPHAKRGG FYGPRGPGHL GGPPGEQRLY SRLRNNDEAQ RIWEISEKLT DMSF
Validated NT Sequence
ggaacgacat gtccgtcaac ttctccgaga tctcccagat acgttgagcc tcatcgttgt tacggagccg cgagtagagc ctctgttctc ccggcggacc accgagatgt ccgggaccac gcggcccgta gaacccaccg cgcttcgcat ggggcgaggc cgcggcgtag agggcaggca gctgcgcggt gtgcacggtc ccgaaaagga tgccacgcga cgacagcgta cggatcaggc gcagtttcca gtcgtcctgt gcgcgtccgg tgtccgggcg ggccgcaagc aggttggtcg gcgccagacc cggatgggac agattgctcg tgatgcccca cccgtgggcg tgactgcgcc ggtccagttc caggccgaag agcccgaacg cgatcttcga ctggctgtac gcctttgcgc cgtcgtacga acgctcccag ttcaaatcgt cccagttgat ggcgtgctga ttcgccgcga cgctgacctg tgaggtcacc cgcgctccac cggcacgcag caggggcagc agatgcgcca cgagggcgaa gtggccgagg tggttggtgc cgaactgcga ctcgaacccg tcggtggtcg tccgccgctc gggcggcgtc atgacaccgg cgttgttgat gagcagatgg atgggctgtg cctcctcgag aaggctcttg ccgagtgccg caacggaggc cagggacgac aggtcgagct cacgcagcga cactttcgcc gcggcgttcg tctgacggac agccgcgatg gccgcctttc ccttgatctc gttgcgtacg ggcaggatca cgtcagcgcc cgccgcggcg agccgcgcgg cgatcccgag tccgatgccg tcgctggcac cggtgaccac ggcgcgtctg ccggacagat cgggaagcgt gacgtcgatg ggtttacgtg gcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat ga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPRKPIDVT LPDLSGRRAV VTGASDGIGL GIAARLAAAG ADVILPVRNE IKGKAAIAAV RQTNAAAKVS LRELDLSSLA SVAALGKSLL EEAQPIHLLI NNAGVMTPPE RRTTTDGFES QFGTNHLGHF ALVAHLLPLL RAGGARVTSQ VSVAANQHAI NWDDLNWERS YDGAKAYSQS KIAFGLFGLE LDRRSHAHGW GITSNLSHPG LAPTNLLAAR PDTGRAQDDW KLRLIRTLSS RGILFGTVHT AQLPALYAAA SPHAKRGGFY GPRGPGHLGG PPGEQRLYSR LRNNDEAQRI WEISEKLTDM SFPTA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccacgtaaa cccatcgacg tcacgcttcc cgatctgtcc ggcagacgcg ccgtggtcac cggtgccagc gacggcatcg gactcgggat cgccgcgcgg ctcgccgcgg cgggcgctga cgtgatcctg cccgtacgca acgagatcaa gggaaaggcg gccatcgcgg ctgtccgtca gacgaacgcc gcggcgaaag tgtcgctgcg tgagctcgac ctgtcgtccc tggcctccgt tgcggcactc ggcaagagcc ttctcgagga ggcacagccc atccatctgc tcatcaacaa cgccggtgtc atgacgccgc ccgagcggcg gacgaccacc gacgggttcg agtcgcagtt cggcaccaac cacctcggcc acttcgccct cgtggcgcat ctgctgcccc tgctgcgtgc cggtggagcg cgggtgacct cacaggtcag cgtcgcggcg aatcagcacg ccatcaactg ggacgatttg aactgggagc gttcgtacga cggcgcaaag gcgtacagcc agtcgaagat cgcgttcggg ctcttcggcc tggaactgga ccggcgcagt cacgcccacg ggtggggcat cacgagcaat ctgtcccatc cgggtctggc gccgaccaac ctgcttgcgg cccgcccgga caccggacgc gcacaggacg actggaaact gcgcctgatc cgtacgctgt cgtcgcgtgg catccttttc gggaccgtgc acaccgcgca gctgcctgcc ctctacgccg cggcctcgcc ccatgcgaag cgcggtgggt tctacgggcc gcgtggtccc ggacatctcg gtggtccgcc gggagaacag aggctctact cgcggctccg taacaacgat gaggctcaac gtatctggga gatctcggag aagttgacgg acatgtcgtt ccccacggca aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa