MysmA.00337.a

Dehydrogenase

CENTER ID: MysmA.00337.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00337.a.A1.GE28609 Full length( MysmA.00337.a ) 1 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00337.a.A1.PW28508 Full length( MysmA.00337.a ) 1 300

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.6382
RefSeq: YP_890770.1
UniProt: A0R6I2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSTWSPDRL GDLGDKRIIV TGATNGVGLG TTRALVRAGA HVILAVRNTD LGARRAAEVG GTTTVVEVDL ADMSSVRAFP GRLTAAGIDD VDILINNAGA LTQRRTETVD GFETTLGTNL LGPFALTNLL LGRVRSQVII VGSDAHRMAT LRLDDMHLRR NRWTPLGAYG RAKLAVMLWG LELDRRQRVA RSPVVTHLTH PGWVASNLPN VSQTRLMSVI QRGVTTVADR FAASIDEGAA PTLYCISEPI PPGSYVGVEG RLGLRGAPVL IGRSAVACDY ETAAAVVDFA ERETGTAIPY
NT Sequence
GTGACGAGCA CATGGTCGCC CGACCGGCTG GGAGATCTCG GCGACAAACG CATCATCGTG ACCGGCGCGA CCAACGGCGT CGGGCTGGGT ACCACGCGCG CACTCGTGCG TGCCGGTGCG CATGTGATCC TCGCGGTGCG CAACACCGAC CTCGGTGCGC GACGTGCCGC TGAGGTCGGC GGCACGACCA CCGTGGTCGA GGTCGACCTC GCCGACATGT CGTCGGTGCG TGCGTTTCCG GGGCGGCTGA CCGCGGCGGG CATCGACGAC GTCGACATCC TGATCAACAA CGCGGGAGCG TTGACGCAGC GTCGTACCGA GACCGTCGAC GGGTTCGAGA CGACGCTGGG CACCAACCTG CTCGGCCCGT TCGCGTTGAC GAATCTGCTT CTCGGCCGCG TCCGCTCGCA GGTGATCATC GTCGGTTCCG ATGCCCACCG GATGGCCACG CTGCGCCTCG ACGACATGCA TCTGCGTCGA AACAGGTGGA CCCCGCTGGG CGCCTACGGC CGAGCCAAGC TCGCGGTGAT GCTGTGGGGC CTGGAACTGG ACAGACGGCA ACGCGTCGCG CGTTCGCCAG TGGTCACACA TCTCACCCAT CCCGGGTGGG TCGCGTCGAA CCTGCCCAAT GTGTCTCAGA CGCGGCTGAT GTCGGTGATC CAGCGCGGGG TCACCACCGT CGCCGACAGG TTCGCCGCCA GCATCGACGA GGGCGCCGCG CCCACCCTGT ACTGCATCAG CGAACCCATC CCGCCCGGCT CCTATGTGGG TGTCGAGGGC AGGCTGGGGT TGCGCGGCGC ACCGGTGCTC ATCGGCCGCT CAGCTGTGGC GTGCGACTAC GAGACCGCCG CGGCTGTGGT GGATTTCGCC GAGCGTGAGA CCGGCACTGC GATCCCTTAC
Details for MysmA.00337.a.A1.GE28609
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSTWSPD RLGDLGDKRI IVTGATNGVG LGTTRALVRA GAHVILAVRN TDLGARRAAE VGGTTTVVEV DLADMSSVRA FPGRLTAAGI DDVDILINNA GALTQRRTET VDGFETTLGT NLLGPFALTN LLLGRVRSQV IIVGSDAHRM ATLRLDDMHL RRNRWTPLGA YGRAKLAVML WGLELDRRQR VARSPVVTHL THPGWVASNL PNVSQTRLMS VIQRGVTTVA DRFAASIDEG AAPTLYCISE PIPPGSYVGV EGRLGLRGAP VLIGRSXVAC DYXTAAAVVX FAXRETGXAI PX
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga cgagcacatg gtcgcccgac cggctgggag atctcggcga caaacgcatc atcgtgaccg gcgcgaccaa cggcgtcggg ctgggtacca cgcgcgcact cgtgcgtgcc ggtgcgcatg tgatcctcgc ggtgcgcaac accgacctcg gtgcgcgacg tgccgctgag gtcggcggca cgaccaccgt ggtcgaggtc gacctcgccg acatgtcgtc ggtgcgtgcg tttccggggc ggctgaccgc ggcgggcatc gacgacgtcg acatcctgat caacaacgcg ggagcgttga cgcagcgtcg taccgagacc gtcgacgggt tcgagacgac gctgggcacc aacctgctcg gcccgttcgc gttgacgaat ctgcttctcg gccgcgtccg ctcgcaggtg atcatcgtcg gttccgatgc ccaccggatg gccacgctgc gcctcgacga catgcatctg cgtcgaaaca ggtggacccc gctgggcgcc tacggccgag ccaagctcgc ggtgatgctg tggggcctgg aactggacag acggcaacgc gtcgcgcgtt cgccagtggt cacacatctc acccatcccg ggtgggtcgc gtcgaacctg cccaatgtgt ctcagacgcg gctgatgtcg gtgatccagc gcggggtcac caccgtcgcc gacaggttcg ccgccagcat cgacgagggc gccgcgccca ccctgtactg catcagcgaa cccatcccgc ccggctccta tgtgggtgtc gagggcaggc tggggttgcg cggcgcaccg gtgctcatcg gccgctcanc tgtggcgtgc gactacnaga ccgccgcggc tgtggtggan ttcgccganc gtgagaccgg cnctgcgatc ccntncta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSTWSPD RLGDLGDKRI IVTGATNGVG LGTTRALVRA GAHVILAVRN TDLGARRAAE VGGTTTVVEV DLADMSSVRA FPGRLTAAGI DDVDILINNA GALTQRRTET VDGFETTLGT NLLGPFALTN LLLGRVRSQV IIVGSDAHRM ATLRLDDMHL RRNRWTPLGA YGRAKLAVML WGLELDRRQR VARSPVVTHL THPGWVASNL PNVSQTRLMS VIQRGVTTVA DRFAASIDEG AAPTLYCISE PIPPGSYVGV EGRLGLRGAP VLIGRSAVAC DYETAAAVVD FAERETGTAI PY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacgagc acatggtcgc ccgaccggct gggagatctc ggcgacaaac gcatcatcgt gaccggcgcg accaacggcg tcgggctggg taccacgcgc gcactcgtgc gtgccggtgc gcatgtgatc ctcgcggtgc gcaacaccga cctcggtgcg cgacgtgccg ctgaggtcgg cggcacgacc accgtggtcg aggtcgacct cgccgacatg tcgtcggtgc gtgcgtttcc ggggcggctg accgcggcgg gcatcgacga cgtcgacatc ctgatcaaca acgcgggagc gttgacgcag cgtcgtaccg agaccgtcga cgggttcgag acgacgctgg gcaccaacct gctcggcccg ttcgcgttga cgaatctgct tctcggccgc gtccgctcgc aggtgatcat cgtcggttcc gatgcccacc ggatggccac gctgcgcctc gacgacatgc atctgcgtcg aaacaggtgg accccgctgg gcgcctacgg ccgagccaag ctcgcggtga tgctgtgggg cctggaactg gacagacggc aacgcgtcgc gcgttcgcca gtggtcacac atctcaccca tcccgggtgg gtcgcgtcga acctgcccaa tgtgtctcag acgcggctga tgtcggtgat ccagcgcggg gtcaccaccg tcgccgacag gttcgccgcc agcatcgacg agggcgccgc gcccaccctg tactgcatca gcgaacccat cccgcccggc tcctatgtgg gtgtcgaggg caggctgggg ttgcgcggcg caccggtgct catcggccgc tcagctgtgg cgtgcgacta cgagaccgcc gcggctgtgg tggatttcgc cgagcgtgag accggcactg cgatccctta caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MysmA.00337.a.A1.PW28508
PURIFICATION DATe: 5/3/2010
CONCENTRATION: 7.25mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 100µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSTWSPD RLGDLGDKRI IVTGATNGVG LGTTRALVRA GAHVILAVRN TDLGARRAAE VGGTTTVVEV DLADMSSVRA FPGRLTAAGI DDVDILINNA GALTQRRTET VDGFETTLGT NLLGPFALTN LLLGRVRSQV IIVGSDAHRM ATLRLDDMHL RRNRWTPLGA YGRAKLAVML WGLELDRRQR VARSPVVTHL THPGWVASNL PNVSQTRLMS VIQRGVTTVA DRFAASIDEG AAPTLYCISE PIPPGSYVGV EGRLGLRGAP VLIGRSXVAC DYXTAAAVVX FAXRETGXAI PX
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga cgagcacatg gtcgcccgac cggctgggag atctcggcga caaacgcatc atcgtgaccg gcgcgaccaa cggcgtcggg ctgggtacca cgcgcgcact cgtgcgtgcc ggtgcgcatg tgatcctcgc ggtgcgcaac accgacctcg gtgcgcgacg tgccgctgag gtcggcggca cgaccaccgt ggtcgaggtc gacctcgccg acatgtcgtc ggtgcgtgcg tttccggggc ggctgaccgc ggcgggcatc gacgacgtcg acatcctgat caacaacgcg ggagcgttga cgcagcgtcg taccgagacc gtcgacgggt tcgagacgac gctgggcacc aacctgctcg gcccgttcgc gttgacgaat ctgcttctcg gccgcgtccg ctcgcaggtg atcatcgtcg gttccgatgc ccaccggatg gccacgctgc gcctcgacga catgcatctg cgtcgaaaca ggtggacccc gctgggcgcc tacggccgag ccaagctcgc ggtgatgctg tggggcctgg aactggacag acggcaacgc gtcgcgcgtt cgccagtggt cacacatctc acccatcccg ggtgggtcgc gtcgaacctg cccaatgtgt ctcagacgcg gctgatgtcg gtgatccagc gcggggtcac caccgtcgcc gacaggttcg ccgccagcat cgacgagggc gccgcgccca ccctgtactg catcagcgaa cccatcccgc ccggctccta tgtgggtgtc gagggcaggc tggggttgcg cggcgcaccg gtgctcatcg gccgctcanc tgtggcgtgc gactacnaga ccgccgcggc tgtggtggan ttcgccganc gtgagaccgg cnctgcgatc ccntncta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTSTWSPD RLGDLGDKRI IVTGATNGVG LGTTRALVRA GAHVILAVRN TDLGARRAAE VGGTTTVVEV DLADMSSVRA FPGRLTAAGI DDVDILINNA GALTQRRTET VDGFETTLGT NLLGPFALTN LLLGRVRSQV IIVGSDAHRM ATLRLDDMHL RRNRWTPLGA YGRAKLAVML WGLELDRRQR VARSPVVTHL THPGWVASNL PNVSQTRLMS VIQRGVTTVA DRFAASIDEG AAPTLYCISE PIPPGSYVGV EGRLGLRGAP VLIGRSAVAC DYETAAAVVD FAERETGTAI PY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACGAGC ACATGGTCGC CCGACCGGCT GGGAGATCTC GGCGACAAAC GCATCATCGT GACCGGCGCG ACCAACGGCG TCGGGCTGGG TACCACGCGC GCACTCGTGC GTGCCGGTGC GCATGTGATC CTCGCGGTGC GCAACACCGA CCTCGGTGCG CGACGTGCCG CTGAGGTCGG CGGCACGACC ACCGTGGTCG AGGTCGACCT CGCCGACATG TCGTCGGTGC GTGCGTTTCC GGGGCGGCTG ACCGCGGCGG GCATCGACGA CGTCGACATC CTGATCAACA ACGCGGGAGC GTTGACGCAG CGTCGTACCG AGACCGTCGA CGGGTTCGAG ACGACGCTGG GCACCAACCT GCTCGGCCCG TTCGCGTTGA CGAATCTGCT TCTCGGCCGC GTCCGCTCGC AGGTGATCAT CGTCGGTTCC GATGCCCACC GGATGGCCAC GCTGCGCCTC GACGACATGC ATCTGCGTCG AAACAGGTGG ACCCCGCTGG GCGCCTACGG CCGAGCCAAG CTCGCGGTGA TGCTGTGGGG CCTGGAACTG GACAGACGGC AACGCGTCGC GCGTTCGCCA GTGGTCACAC ATCTCACCCA TCCCGGGTGG GTCGCGTCGA ACCTGCCCAA TGTGTCTCAG ACGCGGCTGA TGTCGGTGAT CCAGCGCGGG GTCACCACCG TCGCCGACAG GTTCGCCGCC AGCATCGACG AGGGCGCCGC GCCCACCCTG TACTGCATCA GCGAACCCAT CCCGCCCGGC TCCTATGTGG GTGTCGAGGG CAGGCTGGGG TTGCGCGGCG CACCGGTGCT CATCGGCCGC TCAGCTGTGG CGTGCGACTA CGAGACCGCC GCGGCTGTGG TGGATTTCGC CGAGCGTGAG ACCGGCACTG CGATCCCTTA CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA