MysmA.00309.a

Putative acyl-CoA dehydrogenase

CENTER ID: MysmA.00309.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00309.a.A1.GE28605 Full length( MysmA.00309.a ) 1 433
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00309.a.A1.PW28504 Full length( MysmA.00309.a ) 1 433
MysmA.00309.a.A1.PW29674 Full length( MysmA.00309.a ) 1 433

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.4710
RefSeq: YP_889082.1
UniProt: A0R1P4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAWDFETDPQ YQEVLDWADE FVREKVEPLD LVWPHLQFTP LNEARRQVVD PLKEQVRRKG LWATHLGPEL GGQGYGQLKL ALLNEILGRS QWAPIVFGCQ APDTGNAEII AHYGTDEQKE RYLRPLLEGE LFSSYSMTEP HAGADPTLFT TRAVRDGDDW VINGWKFFSS NAATASFLIV MVVTNPDVSA YQGMSMFLVP TDTPGVEIVR NVGLYGERDN EGSHALIHYD NVRVPAEALL GGEGQAFVIA QTRLGGGRIH HAMRTIGLAR KALDMMCERA LSRKTQGSRL SDKQFVQGYI ADSYAQLLQF RLMVLYTAWE IDKYNDYKLV RKDIAAVKVV MPTVLHDIAW RAMQVHGALG VTNEMPFLGM VTGAAVMGLA DGPTEVHKTT VAKQVLRDYQ PTEDTWPTEW IPRKREAARA KLADYLENEV GNL
NT Sequence
ATGGCATGGG ATTTCGAGAC CGACCCCCAA TATCAGGAGG TCCTCGACTG GGCCGACGAG TTCGTGCGGG AGAAGGTCGA ACCGCTGGAC CTCGTGTGGC CGCATCTGCA GTTCACGCCT CTGAACGAGG CACGCCGGCA GGTGGTGGAC CCGCTCAAGG AGCAGGTGCG CCGAAAAGGG CTGTGGGCCA CCCATCTCGG CCCCGAGCTG GGCGGCCAGG GATACGGGCA GCTCAAACTG GCGCTGCTCA ACGAGATCCT GGGCCGGTCG CAGTGGGCGC CCATCGTGTT CGGCTGCCAG GCACCCGATA CCGGCAACGC CGAGATCATC GCGCACTACG GCACCGACGA GCAGAAGGAG CGCTACCTGC GGCCGCTGCT CGAGGGCGAG TTGTTCTCGA GCTACTCGAT GACCGAACCG CACGCCGGGG CGGACCCGAC GCTGTTCACC ACGCGCGCCG TCCGCGACGG GGACGACTGG GTGATCAACG GCTGGAAGTT CTTCTCCTCC AACGCCGCCA CCGCGTCGTT CCTCATCGTC ATGGTCGTCA CCAACCCCGA TGTCAGCGCC TATCAGGGCA TGTCGATGTT CCTGGTGCCC ACCGACACCC CCGGCGTCGA GATCGTGCGC AACGTCGGCC TGTACGGCGA GCGCGACAAC GAGGGATCGC ACGCGCTGAT CCACTACGAC AACGTGCGGG TACCCGCCGA AGCTCTGCTG GGCGGTGAGG GGCAGGCGTT CGTCATCGCC CAGACCCGCT TGGGCGGCGG GCGGATCCAC CACGCCATGC GCACCATCGG CCTCGCACGC AAGGCGCTCG ACATGATGTG CGAACGCGCG CTGAGCCGCA AGACACAGGG AAGCCGTCTG TCCGACAAGC AGTTCGTGCA GGGCTACATC GCCGACTCGT ATGCGCAACT GCTGCAGTTC CGGCTGATGG TGCTCTACAC CGCGTGGGAG ATCGACAAGT ACAACGACTA CAAGTTGGTC CGCAAGGACA TCGCCGCGGT CAAGGTCGTC ATGCCGACCG TGCTGCACGA CATCGCATGG CGCGCGATGC AGGTGCACGG CGCCCTCGGT GTCACCAACG AAATGCCGTT CCTCGGCATG GTCACCGGTG CAGCGGTCAT GGGCCTGGCC GACGGCCCCA CCGAGGTCCA CAAGACCACG GTGGCCAAAC AGGTGCTGCG CGACTATCAG CCCACCGAGG ACACGTGGCC CACCGAGTGG ATCCCCCGTA AGCGCGAGGC CGCCAGGGCC AAACTGGCCG ACTACCTGGA GAACGAGGTG GGCAACCTG
Details for MysmA.00309.a.A1.GE28605
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMAWDFET DPQYQEVLDW ADEFVREKVE PLDLVWPHLQ FTPLNEARRQ VVDPLKEQVR RKGLWATHLG PELGGQGYGQ LKLALLNEIL GRSQWAPIVF GCQAPDTGNA EIIAHYGTDE QKERYLRPLL EGELFSSYSM TEPHAGADPT LFTTRAVRDG DDWVINGWKF FSSNAATASF LIVMVVTNPD VSAYQGMSMF LVPTDTPGVE IVRNVGLYGE RDNEGSHALI HYDNVRVPAE ALLGGEGQAF VIAQTRLGGG RIHHAMRTIG LARKALDMMC ERALSRKTQG SRLSDKQFVQ GYIADSYAQL LQFRLMVLYT AWEIDKYNDY KLVRKDIAAV KVVMPTVLHD IAWRAMQVHG ALGVTNEMPF LGMVTGAAVM GLADGPTEVH KTTVAKQVLR DYQPTEDTWP TEWIPRKREA ARAKLADY
Validated NT Sequence
caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcatgggat ttcgagaccg acccccaata tcaggaggtc ctcgactggg ccgacgagtt cgtgcgggag aaggtcgaac cgctggacct cgtgtggccg catctgcagt tcacgcctct gaacgaggca cgccggcagg tggtggaccc gctcaaggag caggtgcgcc gaaaagggct gtgggccacc catctcggcc ccgagctggg cggccaggga tacgggcagc tcaaactggc gctgctcaac gagatcctgg gccggtcgca gtgggcgccc atcgtgttcg gctgccaggc acccgatacc ggcaacgccg agatcatcgc gcactacggc accgacgagc agaaggagcg ctacctgcgg ccgctgctcg agggcgagtt gttctcgagc tactcgatga ccgaaccgca cgccggggcg gacccgacgc tgttcaccac gcgcgccgtc cgcgacgggg acgactgggt gatcaacggc tggaagttct tctcctccaa cgccgccacc gcgtcgttcc tcatcgtcat ggtcgtcacc aaccccgatg tcagcgccta tcagggcatg tcgatgttcc tggtgcccac cgacaccccc ggcgtcgaga tcgtgcgcaa cgtcggcctg tacggcgagc gcgacaacga gggatcgcac gcgctgatcc actacgacaa cgtgcgggta cccgccgaag ctctgctggg cggtgagggg caggcgttcg tcatcgccca gacccgcttg ggcggcgggc ggatccacca cgccatgcgc accatcggcc tcgcacgcaa ggcgctcgac atgatgtgcg aacgcgcgct gagccgcaag acacagggaa gccgtctgtc cgacaagcag ttcgtgcagg gctacatcgc cgactcgtat gcgcaactgc tgcagttccg gctgatggtg ctctacaccg cgtgggagat cgacaagtac aacgactaca agttggtccg caaggacatc gccgcggtca aggtcgtcat gccgaccgtg ctgcacgaca tcgcatggcg cgcgatgcag gtgcacggcg ccctcggtgt caccaacgaa atgccgttcc tcggcatggt caccggtgca gcggtcatgg gcctggccga cggccccacc gaggtccaca agaccacggt ggccaaacag gtgctgcgcg actatcagcc caccgaggac acgtggccca ccgagtggat cccccgtaag cgcgaggccg ccagggccaa actggccgac tacctg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAWDFETDP QYQEVLDWAD EFVREKVEPL DLVWPHLQFT PLNEARRQVV DPLKEQVRRK GLWATHLGPE LGGQGYGQLK LALLNEILGR SQWAPIVFGC QAPDTGNAEI IAHYGTDEQK ERYLRPLLEG ELFSSYSMTE PHAGADPTLF TTRAVRDGDD WVINGWKFFS SNAATASFLI VMVVTNPDVS AYQGMSMFLV PTDTPGVEIV RNVGLYGERD NEGSHALIHY DNVRVPAEAL LGGEGQAFVI AQTRLGGGRI HHAMRTIGLA RKALDMMCER ALSRKTQGSR LSDKQFVQGY IADSYAQLLQ FRLMVLYTAW EIDKYNDYKL VRKDIAAVKV VMPTVLHDIA WRAMQVHGAL GVTNEMPFLG MVTGAAVMGL ADGPTEVHKT TVAKQVLRDY QPTEDTWPTE WIPRKREAAR AKLADYLENE VGNL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcatgggat ttcgagaccg acccccaata tcaggaggtc ctcgactggg ccgacgagtt cgtgcgggag aaggtcgaac cgctggacct cgtgtggccg catctgcagt tcacgcctct gaacgaggca cgccggcagg tggtggaccc gctcaaggag caggtgcgcc gaaaagggct gtgggccacc catctcggcc ccgagctggg cggccaggga tacgggcagc tcaaactggc gctgctcaac gagatcctgg gccggtcgca gtgggcgccc atcgtgttcg gctgccaggc acccgatacc ggcaacgccg agatcatcgc gcactacggc accgacgagc agaaggagcg ctacctgcgg ccgctgctcg agggcgagtt gttctcgagc tactcgatga ccgaaccgca cgccggggcg gacccgacgc tgttcaccac gcgcgccgtc cgcgacgggg acgactgggt gatcaacggc tggaagttct tctcctccaa cgccgccacc gcgtcgttcc tcatcgtcat ggtcgtcacc aaccccgatg tcagcgccta tcagggcatg tcgatgttcc tggtgcccac cgacaccccc ggcgtcgaga tcgtgcgcaa cgtcggcctg tacggcgagc gcgacaacga gggatcgcac gcgctgatcc actacgacaa cgtgcgggta cccgccgaag ctctgctggg cggtgagggg caggcgttcg tcatcgccca gacccgcttg ggcggcgggc ggatccacca cgccatgcgc accatcggcc tcgcacgcaa ggcgctcgac atgatgtgcg aacgcgcgct gagccgcaag acacagggaa gccgtctgtc cgacaagcag ttcgtgcagg gctacatcgc cgactcgtat gcgcaactgc tgcagttccg gctgatggtg ctctacaccg cgtgggagat cgacaagtac aacgactaca agttggtccg caaggacatc gccgcggtca aggtcgtcat gccgaccgtg ctgcacgaca tcgcatggcg cgcgatgcag gtgcacggcg ccctcggtgt caccaacgaa atgccgttcc tcggcatggt caccggtgca gcggtcatgg gcctggccga cggccccacc gaggtccaca agaccacggt ggccaaacag gtgctgcgcg actatcagcc caccgaggac acgtggccca ccgagtggat cccccgtaag cgcgaggccg ccagggccaa actggccgac tacctggaga acgaggtggg caacctgaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MysmA.00309.a.A1.PW28504
PURIFICATION DATe: 4/26/2010
CONCENTRATION: 28.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMAWDFET DPQYQEVLDW ADEFVREKVE PLDLVWPHLQ FTPLNEARRQ VVDPLKEQVR RKGLWATHLG PELGGQGYGQ LKLALLNEIL GRSQWAPIVF GCQAPDTGNA EIIAHYGTDE QKERYLRPLL EGELFSSYSM TEPHAGADPT LFTTRAVRDG DDWVINGWKF FSSNAATASF LIVMVVTNPD VSAYQGMSMF LVPTDTPGVE IVRNVGLYGE RDNEGSHALI HYDNVRVPAE ALLGGEGQAF VIAQTRLGGG RIHHAMRTIG LARKALDMMC ERALSRKTQG SRLSDKQFVQ GYIADSYAQL LQFRLMVLYT AWEIDKYNDY KLVRKDIAAV KVVMPTVLHD IAWRAMQVHG ALGVTNEMPF LGMVTGAAVM GLADGPTEVH KTTVAKQVLR DYQPTEDTWP TEWIPRKREA ARAKLADY
Validated NT Sequence
caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcatgggat ttcgagaccg acccccaata tcaggaggtc ctcgactggg ccgacgagtt cgtgcgggag aaggtcgaac cgctggacct cgtgtggccg catctgcagt tcacgcctct gaacgaggca cgccggcagg tggtggaccc gctcaaggag caggtgcgcc gaaaagggct gtgggccacc catctcggcc ccgagctggg cggccaggga tacgggcagc tcaaactggc gctgctcaac gagatcctgg gccggtcgca gtgggcgccc atcgtgttcg gctgccaggc acccgatacc ggcaacgccg agatcatcgc gcactacggc accgacgagc agaaggagcg ctacctgcgg ccgctgctcg agggcgagtt gttctcgagc tactcgatga ccgaaccgca cgccggggcg gacccgacgc tgttcaccac gcgcgccgtc cgcgacgggg acgactgggt gatcaacggc tggaagttct tctcctccaa cgccgccacc gcgtcgttcc tcatcgtcat ggtcgtcacc aaccccgatg tcagcgccta tcagggcatg tcgatgttcc tggtgcccac cgacaccccc ggcgtcgaga tcgtgcgcaa cgtcggcctg tacggcgagc gcgacaacga gggatcgcac gcgctgatcc actacgacaa cgtgcgggta cccgccgaag ctctgctggg cggtgagggg caggcgttcg tcatcgccca gacccgcttg ggcggcgggc ggatccacca cgccatgcgc accatcggcc tcgcacgcaa ggcgctcgac atgatgtgcg aacgcgcgct gagccgcaag acacagggaa gccgtctgtc cgacaagcag ttcgtgcagg gctacatcgc cgactcgtat gcgcaactgc tgcagttccg gctgatggtg ctctacaccg cgtgggagat cgacaagtac aacgactaca agttggtccg caaggacatc gccgcggtca aggtcgtcat gccgaccgtg ctgcacgaca tcgcatggcg cgcgatgcag gtgcacggcg ccctcggtgt caccaacgaa atgccgttcc tcggcatggt caccggtgca gcggtcatgg gcctggccga cggccccacc gaggtccaca agaccacggt ggccaaacag gtgctgcgcg actatcagcc caccgaggac acgtggccca ccgagtggat cccccgtaag cgcgaggccg ccagggccaa actggccgac tacctg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAWDFETDP QYQEVLDWAD EFVREKVEPL DLVWPHLQFT PLNEARRQVV DPLKEQVRRK GLWATHLGPE LGGQGYGQLK LALLNEILGR SQWAPIVFGC QAPDTGNAEI IAHYGTDEQK ERYLRPLLEG ELFSSYSMTE PHAGADPTLF TTRAVRDGDD WVINGWKFFS SNAATASFLI VMVVTNPDVS AYQGMSMFLV PTDTPGVEIV RNVGLYGERD NEGSHALIHY DNVRVPAEAL LGGEGQAFVI AQTRLGGGRI HHAMRTIGLA RKALDMMCER ALSRKTQGSR LSDKQFVQGY IADSYAQLLQ FRLMVLYTAW EIDKYNDYKL VRKDIAAVKV VMPTVLHDIA WRAMQVHGAL GVTNEMPFLG MVTGAAVMGL ADGPTEVHKT TVAKQVLRDY QPTEDTWPTE WIPRKREAAR AKLADYLENE VGNL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCATGGGAT TTCGAGACCG ACCCCCAATA TCAGGAGGTC CTCGACTGGG CCGACGAGTT CGTGCGGGAG AAGGTCGAAC CGCTGGACCT CGTGTGGCCG CATCTGCAGT TCACGCCTCT GAACGAGGCA CGCCGGCAGG TGGTGGACCC GCTCAAGGAG CAGGTGCGCC GAAAAGGGCT GTGGGCCACC CATCTCGGCC CCGAGCTGGG CGGCCAGGGA TACGGGCAGC TCAAACTGGC GCTGCTCAAC GAGATCCTGG GCCGGTCGCA GTGGGCGCCC ATCGTGTTCG GCTGCCAGGC ACCCGATACC GGCAACGCCG AGATCATCGC GCACTACGGC ACCGACGAGC AGAAGGAGCG CTACCTGCGG CCGCTGCTCG AGGGCGAGTT GTTCTCGAGC TACTCGATGA CCGAACCGCA CGCCGGGGCG GACCCGACGC TGTTCACCAC GCGCGCCGTC CGCGACGGGG ACGACTGGGT GATCAACGGC TGGAAGTTCT TCTCCTCCAA CGCCGCCACC GCGTCGTTCC TCATCGTCAT GGTCGTCACC AACCCCGATG TCAGCGCCTA TCAGGGCATG TCGATGTTCC TGGTGCCCAC CGACACCCCC GGCGTCGAGA TCGTGCGCAA CGTCGGCCTG TACGGCGAGC GCGACAACGA GGGATCGCAC GCGCTGATCC ACTACGACAA CGTGCGGGTA CCCGCCGAAG CTCTGCTGGG CGGTGAGGGG CAGGCGTTCG TCATCGCCCA GACCCGCTTG GGCGGCGGGC GGATCCACCA CGCCATGCGC ACCATCGGCC TCGCACGCAA GGCGCTCGAC ATGATGTGCG AACGCGCGCT GAGCCGCAAG ACACAGGGAA GCCGTCTGTC CGACAAGCAG TTCGTGCAGG GCTACATCGC CGACTCGTAT GCGCAACTGC TGCAGTTCCG GCTGATGGTG CTCTACACCG CGTGGGAGAT CGACAAGTAC AACGACTACA AGTTGGTCCG CAAGGACATC GCCGCGGTCA AGGTCGTCAT GCCGACCGTG CTGCACGACA TCGCATGGCG CGCGATGCAG GTGCACGGCG CCCTCGGTGT CACCAACGAA ATGCCGTTCC TCGGCATGGT CACCGGTGCA GCGGTCATGG GCCTGGCCGA CGGCCCCACC GAGGTCCACA AGACCACGGT GGCCAAACAG GTGCTGCGCG ACTATCAGCC CACCGAGGAC ACGTGGCCCA CCGAGTGGAT CCCCCGTAAG CGCGAGGCCG CCAGGGCCAA ACTGGCCGAC TACCTGGAGA ACGAGGTGGG CAACCTGAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA
Details for MysmA.00309.a.A1.PW29674
PURIFICATION DATe: 4/26/2010
CONCENTRATION: 5.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMAWDFET DPQYQEVLDW ADEFVREKVE PLDLVWPHLQ FTPLNEARRQ VVDPLKEQVR RKGLWATHLG PELGGQGYGQ LKLALLNEIL GRSQWAPIVF GCQAPDTGNA EIIAHYGTDE QKERYLRPLL EGELFSSYSM TEPHAGADPT LFTTRAVRDG DDWVINGWKF FSSNAATASF LIVMVVTNPD VSAYQGMSMF LVPTDTPGVE IVRNVGLYGE RDNEGSHALI HYDNVRVPAE ALLGGEGQAF VIAQTRLGGG RIHHAMRTIG LARKALDMMC ERALSRKTQG SRLSDKQFVQ GYIADSYAQL LQFRLMVLYT AWEIDKYNDY KLVRKDIAAV KVVMPTVLHD IAWRAMQVHG ALGVTNEMPF LGMVTGAAVM GLADGPTEVH KTTVAKQVLR DYQPTEDTWP TEWIPRKREA ARAKLADY
Validated NT Sequence
caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcatgggat ttcgagaccg acccccaata tcaggaggtc ctcgactggg ccgacgagtt cgtgcgggag aaggtcgaac cgctggacct cgtgtggccg catctgcagt tcacgcctct gaacgaggca cgccggcagg tggtggaccc gctcaaggag caggtgcgcc gaaaagggct gtgggccacc catctcggcc ccgagctggg cggccaggga tacgggcagc tcaaactggc gctgctcaac gagatcctgg gccggtcgca gtgggcgccc atcgtgttcg gctgccaggc acccgatacc ggcaacgccg agatcatcgc gcactacggc accgacgagc agaaggagcg ctacctgcgg ccgctgctcg agggcgagtt gttctcgagc tactcgatga ccgaaccgca cgccggggcg gacccgacgc tgttcaccac gcgcgccgtc cgcgacgggg acgactgggt gatcaacggc tggaagttct tctcctccaa cgccgccacc gcgtcgttcc tcatcgtcat ggtcgtcacc aaccccgatg tcagcgccta tcagggcatg tcgatgttcc tggtgcccac cgacaccccc ggcgtcgaga tcgtgcgcaa cgtcggcctg tacggcgagc gcgacaacga gggatcgcac gcgctgatcc actacgacaa cgtgcgggta cccgccgaag ctctgctggg cggtgagggg caggcgttcg tcatcgccca gacccgcttg ggcggcgggc ggatccacca cgccatgcgc accatcggcc tcgcacgcaa ggcgctcgac atgatgtgcg aacgcgcgct gagccgcaag acacagggaa gccgtctgtc cgacaagcag ttcgtgcagg gctacatcgc cgactcgtat gcgcaactgc tgcagttccg gctgatggtg ctctacaccg cgtgggagat cgacaagtac aacgactaca agttggtccg caaggacatc gccgcggtca aggtcgtcat gccgaccgtg ctgcacgaca tcgcatggcg cgcgatgcag gtgcacggcg ccctcggtgt caccaacgaa atgccgttcc tcggcatggt caccggtgca gcggtcatgg gcctggccga cggccccacc gaggtccaca agaccacggt ggccaaacag gtgctgcgcg actatcagcc caccgaggac acgtggccca ccgagtggat cccccgtaag cgcgaggccg ccagggccaa actggccgac tacctg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAWDFETDP QYQEVLDWAD EFVREKVEPL DLVWPHLQFT PLNEARRQVV DPLKEQVRRK GLWATHLGPE LGGQGYGQLK LALLNEILGR SQWAPIVFGC QAPDTGNAEI IAHYGTDEQK ERYLRPLLEG ELFSSYSMTE PHAGADPTLF TTRAVRDGDD WVINGWKFFS SNAATASFLI VMVVTNPDVS AYQGMSMFLV PTDTPGVEIV RNVGLYGERD NEGSHALIHY DNVRVPAEAL LGGEGQAFVI AQTRLGGGRI HHAMRTIGLA RKALDMMCER ALSRKTQGSR LSDKQFVQGY IADSYAQLLQ FRLMVLYTAW EIDKYNDYKL VRKDIAAVKV VMPTVLHDIA WRAMQVHGAL GVTNEMPFLG MVTGAAVMGL ADGPTEVHKT TVAKQVLRDY QPTEDTWPTE WIPRKREAAR AKLADYLENE VGNL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCATGGGAT TTCGAGACCG ACCCCCAATA TCAGGAGGTC CTCGACTGGG CCGACGAGTT CGTGCGGGAG AAGGTCGAAC CGCTGGACCT CGTGTGGCCG CATCTGCAGT TCACGCCTCT GAACGAGGCA CGCCGGCAGG TGGTGGACCC GCTCAAGGAG CAGGTGCGCC GAAAAGGGCT GTGGGCCACC CATCTCGGCC CCGAGCTGGG CGGCCAGGGA TACGGGCAGC TCAAACTGGC GCTGCTCAAC GAGATCCTGG GCCGGTCGCA GTGGGCGCCC ATCGTGTTCG GCTGCCAGGC ACCCGATACC GGCAACGCCG AGATCATCGC GCACTACGGC ACCGACGAGC AGAAGGAGCG CTACCTGCGG CCGCTGCTCG AGGGCGAGTT GTTCTCGAGC TACTCGATGA CCGAACCGCA CGCCGGGGCG GACCCGACGC TGTTCACCAC GCGCGCCGTC CGCGACGGGG ACGACTGGGT GATCAACGGC TGGAAGTTCT TCTCCTCCAA CGCCGCCACC GCGTCGTTCC TCATCGTCAT GGTCGTCACC AACCCCGATG TCAGCGCCTA TCAGGGCATG TCGATGTTCC TGGTGCCCAC CGACACCCCC GGCGTCGAGA TCGTGCGCAA CGTCGGCCTG TACGGCGAGC GCGACAACGA GGGATCGCAC GCGCTGATCC ACTACGACAA CGTGCGGGTA CCCGCCGAAG CTCTGCTGGG CGGTGAGGGG CAGGCGTTCG TCATCGCCCA GACCCGCTTG GGCGGCGGGC GGATCCACCA CGCCATGCGC ACCATCGGCC TCGCACGCAA GGCGCTCGAC ATGATGTGCG AACGCGCGCT GAGCCGCAAG ACACAGGGAA GCCGTCTGTC CGACAAGCAG TTCGTGCAGG GCTACATCGC CGACTCGTAT GCGCAACTGC TGCAGTTCCG GCTGATGGTG CTCTACACCG CGTGGGAGAT CGACAAGTAC AACGACTACA AGTTGGTCCG CAAGGACATC GCCGCGGTCA AGGTCGTCAT GCCGACCGTG CTGCACGACA TCGCATGGCG CGCGATGCAG GTGCACGGCG CCCTCGGTGT CACCAACGAA ATGCCGTTCC TCGGCATGGT CACCGGTGCA GCGGTCATGG GCCTGGCCGA CGGCCCCACC GAGGTCCACA AGACCACGGT GGCCAAACAG GTGCTGCGCG ACTATCAGCC CACCGAGGAC ACGTGGCCCA CCGAGTGGAT CCCCCGTAAG CGCGAGGCCG CCAGGGCCAA ACTGGCCGAC TACCTGGAGA ACGAGGTGGG CAACCTGAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA