MysmA.00305.b

Enoyl-CoA hydratase/isomerase

CENTER ID: MysmA.00305.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00305.b.A1.GE28604 Full length( MysmA.00305.b ) 1 273
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00305.b.A1.PS00801 Full length( MysmA.00305.b ) 1 273

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.3099
RefSeq: YP_887454.1
UniProt: A0QX16

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDDTRFVLL DRSRPGVALV TLNRPERMNS MAFDVMVPLL ELLGDLRHDN SVRVVILTGA GRGFSSGADH KSAGSVPHVD GLTRPTYALR SMEVLDNVIL ALRRLHQPVI AAVNGAAIGG GLCLALACDV RIAAHNAYFR AAGINNGLTA SELGLSYLLP RAIGTSRAFE IMLTGRDVDA AEAERIGLVS RTVPDDDLLE TCFEMAQRMR GFSRPGIELT KRTLWSGLDA ASLEGHMQAE GLGQLFVRLL TANFEEAVAA RAEKRPPAFT DDK
NT Sequence
GTGACTGACG ACACTCGCTT TGTTCTACTG GACCGGTCGC GGCCGGGCGT GGCCCTCGTG ACGCTCAACC GCCCCGAGCG GATGAACTCG ATGGCGTTCG ACGTCATGGT GCCGCTGCTG GAGCTGCTGG GCGACCTTCG CCATGACAAC TCGGTGCGTG TGGTCATTCT CACCGGCGCC GGGCGCGGAT TCTCCTCGGG CGCCGACCAC AAGTCGGCGG GCTCCGTGCC GCACGTCGAC GGTCTGACCC GCCCGACGTA CGCGCTGCGG TCGATGGAAG TGCTCGACAA CGTGATCCTG GCGCTGCGCC GGCTGCACCA GCCCGTGATC GCCGCGGTCA ACGGCGCGGC CATCGGCGGG GGATTGTGCC TCGCGCTGGC GTGCGACGTC CGGATCGCTG CTCACAACGC CTATTTCCGG GCGGCCGGGA TCAACAACGG CCTCACCGCG AGCGAGCTGG GCCTGTCGTA TCTGCTGCCG AGGGCGATCG GCACGTCACG CGCCTTCGAG ATCATGCTGA CCGGACGCGA CGTCGACGCC GCGGAAGCCG AGCGCATCGG TCTGGTGTCG CGCACGGTCC CCGACGACGA CCTTCTCGAG ACCTGTTTCG AGATGGCCCA GCGCATGCGC GGGTTCTCGC GACCGGGAAT CGAGTTGACC AAGCGCACAC TTTGGAGTGG ACTGGACGCC GCTAGTCTGG AGGGGCACAT GCAGGCCGAA GGCCTGGGCC AACTCTTCGT GCGTCTGCTC ACCGCAAACT TTGAGGAAGC GGTGGCCGCG CGCGCCGAGA AGCGCCCCCC GGCATTCACC GACGACAAG
Details for MysmA.00305.b.A1.GE28604
HARVESTED ON: 4/9/2010
SEQUENCED ON: 4/29/2010
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTDDTRFV LLDRSRPGVA LVTLNRPERM NSMAFDVMVP LLELLGDLRH DNSVRVVILT GAGRGFSSGA DHKSAGSVPH VDGLTRPTYA LRSMEVLDNV ILALRRLHQP VIAAVNGAAI GGGLCLALAC DVRIAAHNAY FRAAGINNGL TASELGLSYL LPRAIGTSRA FEIMLTGRDV DAAEAERIGL VSRTVPDDDL LETCFEMAQR MRGFSRPGIE LTKRTLWSGL DAASLEGHMQ AEGLGQLFVR LLTANFEEAV AARAEKRPPA FTD
Validated NT Sequence
cgtcggtgaa tgccgggggg cgcttctcgg cgcgcgcggc caccgcttcc tcaaagtttg cggtgagcag acgcacgaag agttggccca ggccttcggc ctgcatgtgc ccctccagac tagcggcgtc cagtccactc caaagtgtgc gcttggtcaa ctcgattccc ggtcgcgaga acccgcgcat gcgctgggcc atctcgaaac aggtctcgag aaggtcgtcg tcggggaccg tgcgcgacac cagaccgatg cgctcggctt ccgcggcgtc gacgtcgcgt ccggtcagca tgatctcgaa ggcgcgtgac gtgccgatcg ccctcggcag cagatacgac aggcccagct cgctcgcggt gaggccgttg ttgatcccgg ccgcccggaa ataggcgttg tgagcagcga tccggacgtc gcacgccagc gcgaggcaca atcccccgcc gatggccgcg ccgttgaccg cggcgatcac gggctggtgc agccggcgca gcgccaggat cacgttgtcg agcacttcca tcgaccgcag cgcgtacgtc gggcgggtca gaccgtcgac gtgcggcacg gagcccgccg acttgtggtc ggcgcccgag gagaatccgc gcccggcgcc ggtgagaatg accacacgca ccgagttgtc atggcgaagg tcgcccagca gctccagcag cggcaccatg acgtcgaacg ccatcgagtt catccgctcg gggcggttga gcgtcacgag ggccacgccc ggccgcgacc ggtccagtag aacaaagcga gtgtcgtcag tcaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat ggtatntctc cttct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTDDTRFV LLDRSRPGVA LVTLNRPERM NSMAFDVMVP LLELLGDLRH DNSVRVVILT GAGRGFSSGA DHKSAGSVPH VDGLTRPTYA LRSMEVLDNV ILALRRLHQP VIAAVNGAAI GGGLCLALAC DVRIAAHNAY FRAAGINNGL TASELGLSYL LPRAIGTSRA FEIMLTGRDV DAAEAERIGL VSRTVPDDDL LETCFEMAQR MRGFSRPGIE LTKRTLWSGL DAASLEGHMQ AEGLGQLFVR LLTANFEEAV AARAEKRPPA FTDDK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgactgac gacactcgct ttgttctact ggaccggtcg cggccgggcg tggccctcgt gacgctcaac cgccccgagc ggatgaactc gatggcgttc gacgtcatgg tgccgctgct ggagctgctg ggcgaccttc gccatgacaa ctcggtgcgt gtggtcattc tcaccggcgc cgggcgcgga ttctcctcgg gcgccgacca caagtcggcg ggctccgtgc cgcacgtcga cggtctgacc cgcccgacgt acgcgctgcg gtcgatggaa gtgctcgaca acgtgatcct ggcgctgcgc cggctgcacc agcccgtgat cgccgcggtc aacggcgcgg ccatcggcgg gggattgtgc ctcgcgctgg cgtgcgacgt ccggatcgct gctcacaacg cctatttccg ggcggccggg atcaacaacg gcctcaccgc gagcgagctg ggcctgtcgt atctgctgcc gagggcgatc ggcacgtcac gcgccttcga gatcatgctg accggacgcg acgtcgacgc cgcggaagcc gagcgcatcg gtctggtgtc gcgcacggtc cccgacgacg accttctcga gacctgtttc gagatggccc agcgcatgcg cgggttctcg cgaccgggaa tcgagttgac caagcgcaca ctttggagtg gactggacgc cgctagtctg gaggggcaca tgcaggccga aggcctgggc caactcttcg tgcgtctgct caccgcaaac tttgaggaag cggtggccgc gcgcgccgag aagcgccccc cggcattcac cgacgacaag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MysmA.00305.b.A1.PS00801
PURIFICATION DATe: 10/11/2010
CONCENTRATION: 92.29mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTDDTRFV LLDRSRPGVA LVTLNRPERM NSMAFDVMVP LLELLGDLRH DNSVRVVILT GAGRGFSSGA DHKSAGSVPH VDGLTRPTYA LRSMEVLDNV ILALRRLHQP VIAAVNGAAI GGGLCLALAC DVRIAAHNAY FRAAGINNGL TASELGLSYL LPRAIGTSRA FEIMLTGRDV DAAEAERIGL VSRTVPDDDL LETCFEMAQR MRGFSRPGIE LTKRTLWSGL DAASLEGHMQ AEGLGQLFVR LLTANFEEAV AARAEKRPPA FTD
Validated NT Sequence
cgtcggtgaa tgccgggggg cgcttctcgg cgcgcgcggc caccgcttcc tcaaagtttg cggtgagcag acgcacgaag agttggccca ggccttcggc ctgcatgtgc ccctccagac tagcggcgtc cagtccactc caaagtgtgc gcttggtcaa ctcgattccc ggtcgcgaga acccgcgcat gcgctgggcc atctcgaaac aggtctcgag aaggtcgtcg tcggggaccg tgcgcgacac cagaccgatg cgctcggctt ccgcggcgtc gacgtcgcgt ccggtcagca tgatctcgaa ggcgcgtgac gtgccgatcg ccctcggcag cagatacgac aggcccagct cgctcgcggt gaggccgttg ttgatcccgg ccgcccggaa ataggcgttg tgagcagcga tccggacgtc gcacgccagc gcgaggcaca atcccccgcc gatggccgcg ccgttgaccg cggcgatcac gggctggtgc agccggcgca gcgccaggat cacgttgtcg agcacttcca tcgaccgcag cgcgtacgtc gggcgggtca gaccgtcgac gtgcggcacg gagcccgccg acttgtggtc ggcgcccgag gagaatccgc gcccggcgcc ggtgagaatg accacacgca ccgagttgtc atggcgaagg tcgcccagca gctccagcag cggcaccatg acgtcgaacg ccatcgagtt catccgctcg gggcggttga gcgtcacgag ggccacgccc ggccgcgacc ggtccagtag aacaaagcga gtgtcgtcag tcaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat ggtatntctc cttct
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTDDTRFV LLDRSRPGVA LVTLNRPERM NSMAFDVMVP LLELLGDLRH DNSVRVVILT GAGRGFSSGA DHKSAGSVPH VDGLTRPTYA LRSMEVLDNV ILALRRLHQP VIAAVNGAAI GGGLCLALAC DVRIAAHNAY FRAAGINNGL TASELGLSYL LPRAIGTSRA FEIMLTGRDV DAAEAERIGL VSRTVPDDDL LETCFEMAQR MRGFSRPGIE LTKRTLWSGL DAASLEGHMQ AEGLGQLFVR LLTANFEEAV AARAEKRPPA FTDDK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACTGAC GACACTCGCT TTGTTCTACT GGACCGGTCG CGGCCGGGCG TGGCCCTCGT GACGCTCAAC CGCCCCGAGC GGATGAACTC GATGGCGTTC GACGTCATGG TGCCGCTGCT GGAGCTGCTG GGCGACCTTC GCCATGACAA CTCGGTGCGT GTGGTCATTC TCACCGGCGC CGGGCGCGGA TTCTCCTCGG GCGCCGACCA CAAGTCGGCG GGCTCCGTGC CGCACGTCGA CGGTCTGACC CGCCCGACGT ACGCGCTGCG GTCGATGGAA GTGCTCGACA ACGTGATCCT GGCGCTGCGC CGGCTGCACC AGCCCGTGAT CGCCGCGGTC AACGGCGCGG CCATCGGCGG GGGATTGTGC CTCGCGCTGG CGTGCGACGT CCGGATCGCT GCTCACAACG CCTATTTCCG GGCGGCCGGG ATCAACAACG GCCTCACCGC GAGCGAGCTG GGCCTGTCGT ATCTGCTGCC GAGGGCGATC GGCACGTCAC GCGCCTTCGA GATCATGCTG ACCGGACGCG ACGTCGACGC CGCGGAAGCC GAGCGCATCG GTCTGGTGTC GCGCACGGTC CCCGACGACG ACCTTCTCGA GACCTGTTTC GAGATGGCCC AGCGCATGCG CGGGTTCTCG CGACCGGGAA TCGAGTTGAC CAAGCGCACA CTTTGGAGTG GACTGGACGC CGCTAGTCTG GAGGGGCACA TGCAGGCCGA AGGCCTGGGC CAACTCTTCG TGCGTCTGCT CACCGCAAAC TTTGAGGAAG CGGTGGCCGC GCGCGCCGAG AAGCGCCCCC CGGCATTCAC CGACGACAAG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA