MysmA.00305.a

Enoyl-CoA hydratase/isomerase family protein

CENTER ID: MysmA.00305.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00305.a.A1.GE28603 Full length( MysmA.00305.a ) 1 262
MysmA.00305.a.AE1.GE43156 Full length( MysmA.00305.a ) 1 262
MysmA.00305.a.B1.GE43079 Full length( MysmA.00305.a ) 1 262
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00305.a.A1.PS00528 Full length( MysmA.00305.a ) 1 262

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.1724
RefSeq: YP_886112.1
UniProt: A0QT74

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLAQPLDVT RPRPDIAVLT LNRPDKLNAL SYELVEALHA ALDDIAADND CRVVVLTGAG RGFCSGLDLS APNPPEAAGG TEFPRSGMRW QERIANLTAK LHRLRQPVIA AVNGPAYGGG FALAAASDIR LAAESARFCT QFIKLGIGGC DIGVSYTLPR IIGAGPAFDL ILTARAVDAT EALRLGLVSR VCADVTAEAM DIAETLCGYG KFGVESTKQV LWANLDAGCL EAALHVENRS QILASTEGGV RAFSEEFRRR PR
NT Sequence
ATGTCGCTTG CACAGCCGCT CGATGTCACC CGTCCGCGCC CCGATATCGC CGTCCTCACG CTCAACCGGC CCGACAAGCT CAATGCGCTG TCGTACGAAC TCGTCGAGGC GCTGCACGCC GCCCTCGACG ACATCGCCGC CGACAACGAC TGCCGCGTCG TGGTGCTCAC CGGGGCCGGT CGCGGCTTCT GTTCGGGTCT GGACCTGAGT GCGCCGAATC CGCCCGAGGC CGCAGGCGGG ACGGAGTTCC CGCGCTCGGG CATGCGGTGG CAGGAGCGCA TCGCGAATCT CACGGCGAAA CTGCACCGGC TGCGTCAGCC GGTGATCGCC GCGGTCAACG GCCCGGCCTA CGGCGGCGGT TTCGCACTGG CGGCCGCGAG CGACATCCGG CTTGCCGCCG AATCGGCGCG CTTCTGCACG CAGTTCATCA AGCTCGGCAT CGGTGGATGC GACATCGGCG TCAGCTACAC CCTGCCCCGG ATCATCGGTG CGGGCCCGGC TTTCGACCTG ATCCTCACCG CGCGTGCGGT CGACGCCACC GAAGCCCTAC GGCTCGGGCT GGTTTCGCGC GTGTGCGCCG ACGTGACCGC CGAGGCCATG GATATCGCTG AAACGCTCTG TGGCTACGGC AAATTCGGCG TCGAATCGAC CAAACAGGTG CTGTGGGCGA ACCTGGATGC CGGATGCCTC GAGGCCGCGC TGCACGTCGA GAACCGCAGT CAGATCCTGG CGTCGACCGA AGGCGGGGTA CGCGCGTTCA GCGAGGAGTT CCGCCGCCGT CCACGA
Details for MysmA.00305.a.A1.GE28603
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSLAQPLDV TRPRPDIAVL TLNRPDKLNA LSYELVEALH AALDDIAADN DCRVVVLTGA GRGFCSGLDL SAPNPPEAAG GTEFPRSGMR WQERIANLTA KLHRLRQPVI AAVNGPAYGG GFALAAASDI RLAAESARFC TQFIKLGIGG CDIGVSYTLP RIIGAGPAFD LILTARAVDA TEALRLGLVS RVCADVTAEA MDIAETLCGY GKFGVESTKQ VLWANLDAGC LEAALHVENR SQILASTEGG VRAFSEEFRR RPR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcgc ttgcacagcc gctcgatgtc acccgtccgc gccccgatat cgccgtcctc acgctcaacc ggcccgacaa gctcaatgcg ctgtcgtacg aactcgtcga ggcgctgcac gccgccctcg acgacatcgc cgccgacaac gactgccgcg tcgtggtgct caccggggcc ggtcgcggct tctgttcggg tctggacctg agtgcgccga atccgcccga ggccgcaggc gggacggagt tcccgcgctc gggcatgcgg tggcaggagc gcatcgcgaa tctcacggcg aaactgcacc ggctgcgtca gccggtgatc gccgcggtca acggcccggc ctacggcggc ggtttcgcac tggcggccgc gagcgacatc cggcttgccg ccgaatcggc gcgcttctgc acgcagttca tcaagctcgg catcggtgga tgcgacatcg gcgtcagcta caccctgccc cggatcatcg gtgcgggccc ggctttcgac ctgatcctca ccgcgcgtgc ggtcgacgcc accgaagccc tacggctcgg gctggtttcg cgcgtgtgcg ccgacgtgac cgccgaggcc atggatatcg ctgaaacgct ctgtggctac ggcaaattcg gcgtcgaatc gaccaaacag gtgctgtggg cgaacctgga tgccggatgc ctcgaggccg cgctgcacgt cgagaaccgc agtcagatcc tggcgtcgac cgaaggcggg gtacgcgcgt tcagcgagga gttccgccgc cgtccacgat aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLAQPLDV TRPRPDIAVL TLNRPDKLNA LSYELVEALH AALDDIAADN DCRVVVLTGA GRGFCSGLDL SAPNPPEAAG GTEFPRSGMR WQERIANLTA KLHRLRQPVI AAVNGPAYGG GFALAAASDI RLAAESARFC TQFIKLGIGG CDIGVSYTLP RIIGAGPAFD LILTARAVDA TEALRLGLVS RVCADVTAEA MDIAETLCGY GKFGVESTKQ VLWANLDAGC LEAALHVENR SQILASTEGG VRAFSEEFRR RPR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgcttgca cagccgctcg atgtcacccg tccgcgcccc gatatcgccg tcctcacgct caaccggccc gacaagctca atgcgctgtc gtacgaactc gtcgaggcgc tgcacgccgc cctcgacgac atcgccgccg acaacgactg ccgcgtcgtg gtgctcaccg gggccggtcg cggcttctgt tcgggtctgg acctgagtgc gccgaatccg cccgaggccg caggcgggac ggagttcccg cgctcgggca tgcggtggca ggagcgcatc gcgaatctca cggcgaaact gcaccggctg cgtcagccgg tgatcgccgc ggtcaacggc ccggcctacg gcggcggttt cgcactggcg gccgcgagcg acatccggct tgccgccgaa tcggcgcgct tctgcacgca gttcatcaag ctcggcatcg gtggatgcga catcggcgtc agctacaccc tgccccggat catcggtgcg ggcccggctt tcgacctgat cctcaccgcg cgtgcggtcg acgccaccga agccctacgg ctcgggctgg tttcgcgcgt gtgcgccgac gtgaccgccg aggccatgga tatcgctgaa acgctctgtg gctacggcaa attcggcgtc gaatcgacca aacaggtgct gtgggcgaac ctggatgccg gatgcctcga ggccgcgctg cacgtcgaga accgcagtca gatcctggcg tcgaccgaag gcggggtacg cgcgttcagc gaggagttcc gccgccgtcc acgaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MysmA.00305.a.AE1.GE43156
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 68
Validated AA Sequence
MRWQERIANL TAKLHRLRQP VIAAVNGPAY GGGFALAAAS DIRLAAESAR FCTQFIKLGI GGCDIGVSYT LPRIIGAGPA FDLILTARAV DATEALRLGL VSRVCADVTA EAMDIAETLC GYGKFGVEST KQVLWANLDA GCLEAALHVE NRSQILASTE GGVRAFSEEF RRRPRAGHHH HHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag ctcgtggacg gcggcggaac tcctcgctga acgcgcgtac cccgccttcg gtcgacgcca ggatctgact gcggttctcg acgtgcagcg cggcctcgag gcatccggca tccaggttcg cccacagcac ctgtttggtc gattcgacgc cgaatttgcc gtagccacag agcgtttcag cgatatccat ggcctcggcg gtcacgtcgg cgcacacgcg cgaaaccagc ccgagccgta gggcttcggt ggcgtcgacc gcacgcgcgg tgaggatcag gtcgaaagcc gggcccgcac cgatgatccg gggcagggtg tagctgacgc cgatgtcgca tccaccgatg ccgagcttga tgaactgcgt gcagaagcgc gccgattcgg cggcaagccg gatgtcgctc gcggccgcca gtgcgaaacc gccgccgtag gccgggccgt tgaccgcggc gatcaccggc tgacgcagcc ggtgcagttt cgccgtgaga ttcgcgatgc gctcctgcca ccgcatgccc gagcgcggga actccgtccc gcctgcggcc tcgggcggat tcggcgcact caggtccaga cccgaacaga agccgcgacc ggccccggtg agcaccacga cgcggcagtc gttgtcggcg gcgatgtcgt cgagggcggc gtgcagcgcc tcgacgagtt cgtacgacag cgcattgagc ttgtcgggcc ggttgagcgt gaggacggcg atatcggggc gcggacgggt gacatcg
Expected Protein Sequence
MSLAQPLDVT RPRPDIAVLT LNRPDKLNAL SYELVEALHA ALDDIAADND CRVVVLTGAG RGFCSGLDLS APNPPEAAGG TEFPRSGMRW QERIANLTAK LHRLRQPVIA AVNGPAYGGG FALAAASDIR LAAESARFCT QFIKLGIGGC DIGVSYTLPR IIGAGPAFDL ILTARAVDAT EALRLGLVSR VCADVTAEAM DIAETLCGYG KFGVESTKQV LWANLDAGCL EAALHVENRS QILASTEGGV RAFSEEFRRR PRGHHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gtcgcttgca cagccgctcg atgtcacccg tccgcgcccc gatatcgccg tcctcacgct caaccggccc gacaagctca atgcgctgtc gtacgaactc gtcgaggcgc tgcacgccgc cctcgacgac atcgccgccg acaacgactg ccgcgtcgtg gtgctcaccg gggccggtcg cggcttctgt tcgggtctgg acctgagtgc gccgaatccg cccgaggccg caggcgggac ggagttcccg cgctcgggca tgcggtggca ggagcgcatc gcgaatctca cggcgaaact gcaccggctg cgtcagccgg tgatcgccgc ggtcaacggc ccggcctacg gcggcggttt cgcactggcg gccgcgagcg acatccggct tgccgccgaa tcggcgcgct tctgcacgca gttcatcaag ctcggcatcg gtggatgcga catcggcgtc agctacaccc tgccccggat catcggtgcg ggcccggctt tcgacctgat cctcaccgcg cgtgcggtcg acgccaccga agccctacgg ctcgggctgg tttcgcgcgt gtgcgccgac gtgaccgccg aggccatgga tatcgctgaa acgctctgtg gctacggcaa attcggcgtc gaatcgacca aacaggtgct gtgggcgaac ctggatgccg gatgcctcga ggccgcgctg cacgtcgaga accgcagtca gatcctggcg tcgaccgaag gcggggtacg cgcgttcagc gaggagttcc gccgccgtcc acgagggcac caccatcatc atcattaacg gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at
Details for MysmA.00305.a.B1.GE43079
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 29kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 91
PERCENT COVERAGE: 94
Validated AA Sequence
MSXVRAPISP SSXSTGXXKL NALSYELVEA LHAALDDIAA DNDCRVVVLT GAGRGFCSGL DLSAPNPPEA AGGTEFPRSG MRWQERIANL TAKLHRLRQP VIAAVNGPAY GGGFALAAAS DIRLAAESAR FCTQFIKLGI GGCDIGVSYT LPRIIGAGPA FDLILTARAV DATEALRLGL VSRVCADVTA EAMDIAETLC GYGKFGVEST KQVLWANLDA GCLEAALHVE NRSQILASTE GGVRAXXXXX RRRPR
Validated NT Sequence
atcttactca cttatcgtgg acggcggcgn nnnnnnncnn nnaacgcgcg taccccgcct tcggtcgacg ccaggatctg actgcggttc tcgacgtgca gcgcggcctc gaggcatccg gcatccaggt tcgcccacag cacctgtttg gtcgattcga cgccgaattt gccgtagcca cagagcgttt cagcgatatc catggcctcg gcggtcacgt cggcgcacac gcgcgaaacc agcccgagcc gtagggcttc ggtggcgtcg accgcacgcg cggtgaggat caggtcgaaa gccgggcccg caccgatgat ccggggcagg gtgtagctga cgccgatgtc gcatccaccg atgccgagct tgatgaactg cgtgcagaag cgcgccgatt cggcggcaag ccggatgtcg ctcgcggccg ccagtgcgaa accgccgccg taggccgggc cgttgaccgc ggcgatcacc ggctgacgca gccggtgcag tttcgccgtg agattcgcga tgcgctcctg ccaccgcatg cccgagcgcg ggaactccgt cccgcctgcg gcctcgggcg gattcggcgc actcaggtcc agacccgaac agaagccgcg accggccccg gtgagcacca cgacgcggca gtcgttgtcg gcggcgatgt cgtcgagggc ggcgtgcagc gcctcgacga gttcgtacga cagcgcattg agcttnncnn ggccggttga gngtgaggac ggcgatatcg gggcncggac nngtgacatc gagcggctgt gcaagcgaca tatggtggtg gtgg
Expected Protein Sequence
MAHHHHHHMS LAQPLDVTRP RPDIAVLTLN RPDKLNALSY ELVEALHAAL DDIAADNDCR VVVLTGAGRG FCSGLDLSAP NPPEAAGGTE FPRSGMRWQE RIANLTAKLH RLRQPVIAAV NGPAYGGGFA LAAASDIRLA AESARFCTQF IKLGIGGCDI GVSYTLPRII GAGPAFDLIL TARAVDATEA LRLGLVSRVC ADVTAEAMDI AETLCGYGKF GVESTKQVLW ANLDAGCLEA ALHVENRSQI LASTEGGVRA FSEEFRRRPR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgc ttgcacagcc gctcgatgtc acccgtccgc gccccgatat cgccgtcctc acgctcaacc ggcccgacaa gctcaatgcg ctgtcgtacg aactcgtcga ggcgctgcac gccgccctcg acgacatcgc cgccgacaac gactgccgcg tcgtggtgct caccggggcc ggtcgcggct tctgttcggg tctggacctg agtgcgccga atccgcccga ggccgcaggc gggacggagt tcccgcgctc gggcatgcgg tggcaggagc gcatcgcgaa tctcacggcg aaactgcacc ggctgcgtca gccggtgatc gccgcggtca acggcccggc ctacggcggc ggtttcgcac tggcggccgc gagcgacatc cggcttgccg ccgaatcggc gcgcttctgc acgcagttca tcaagctcgg catcggtgga tgcgacatcg gcgtcagcta caccctgccc cggatcatcg gtgcgggccc ggctttcgac ctgatcctca ccgcgcgtgc ggtcgacgcc accgaagccc tacggctcgg gctggtttcg cgcgtgtgcg ccgacgtgac cgccgaggcc atggatatcg ctgaaacgct ctgtggctac ggcaaattcg gcgtcgaatc gaccaaacag gtgctgtggg cgaacctgga tgccggatgc ctcgaggccg cgctgcacgt cgagaaccgc agtcagatcc tggcgtcgac cgaaggcggg gtacgcgcgt tcagcgagga gttccgccgc cgtccacgat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for MysmA.00305.a.A1.PS00528
PURIFICATION DATe: 2/17/2010
CONCENTRATION: 44.88mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSLAQPLDV TRPRPDIAVL TLNRPDKLNA LSYELVEALH AALDDIAADN DCRVVVLTGA GRGFCSGLDL SAPNPPEAAG GTEFPRSGMR WQERIANLTA KLHRLRQPVI AAVNGPAYGG GFALAAASDI RLAAESARFC TQFIKLGIGG CDIGVSYTLP RIIGAGPAFD LILTARAVDA TEALRLGLVS RVCADVTAEA MDIAETLCGY GKFGVESTKQ VLWANLDAGC LEAALHVENR SQILASTEGG VRAFSEEFRR RPR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcgc ttgcacagcc gctcgatgtc acccgtccgc gccccgatat cgccgtcctc acgctcaacc ggcccgacaa gctcaatgcg ctgtcgtacg aactcgtcga ggcgctgcac gccgccctcg acgacatcgc cgccgacaac gactgccgcg tcgtggtgct caccggggcc ggtcgcggct tctgttcggg tctggacctg agtgcgccga atccgcccga ggccgcaggc gggacggagt tcccgcgctc gggcatgcgg tggcaggagc gcatcgcgaa tctcacggcg aaactgcacc ggctgcgtca gccggtgatc gccgcggtca acggcccggc ctacggcggc ggtttcgcac tggcggccgc gagcgacatc cggcttgccg ccgaatcggc gcgcttctgc acgcagttca tcaagctcgg catcggtgga tgcgacatcg gcgtcagcta caccctgccc cggatcatcg gtgcgggccc ggctttcgac ctgatcctca ccgcgcgtgc ggtcgacgcc accgaagccc tacggctcgg gctggtttcg cgcgtgtgcg ccgacgtgac cgccgaggcc atggatatcg ctgaaacgct ctgtggctac ggcaaattcg gcgtcgaatc gaccaaacag gtgctgtggg cgaacctgga tgccggatgc ctcgaggccg cgctgcacgt cgagaaccgc agtcagatcc tggcgtcgac cgaaggcggg gtacgcgcgt tcagcgagga gttccgccgc cgtccacgat aataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLAQPLDV TRPRPDIAVL TLNRPDKLNA LSYELVEALH AALDDIAADN DCRVVVLTGA GRGFCSGLDL SAPNPPEAAG GTEFPRSGMR WQERIANLTA KLHRLRQPVI AAVNGPAYGG GFALAAASDI RLAAESARFC TQFIKLGIGG CDIGVSYTLP RIIGAGPAFD LILTARAVDA TEALRLGLVS RVCADVTAEA MDIAETLCGY GKFGVESTKQ VLWANLDAGC LEAALHVENR SQILASTEGG VRAFSEEFRR RPR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGCTTGCA CAGCCGCTCG ATGTCACCCG TCCGCGCCCC GATATCGCCG TCCTCACGCT CAACCGGCCC GACAAGCTCA ATGCGCTGTC GTACGAACTC GTCGAGGCGC TGCACGCCGC CCTCGACGAC ATCGCCGCCG ACAACGACTG CCGCGTCGTG GTGCTCACCG GGGCCGGTCG CGGCTTCTGT TCGGGTCTGG ACCTGAGTGC GCCGAATCCG CCCGAGGCCG CAGGCGGGAC GGAGTTCCCG CGCTCGGGCA TGCGGTGGCA GGAGCGCATC GCGAATCTCA CGGCGAAACT GCACCGGCTG CGTCAGCCGG TGATCGCCGC GGTCAACGGC CCGGCCTACG GCGGCGGTTT CGCACTGGCG GCCGCGAGCG ACATCCGGCT TGCCGCCGAA TCGGCGCGCT TCTGCACGCA GTTCATCAAG CTCGGCATCG GTGGATGCGA CATCGGCGTC AGCTACACCC TGCCCCGGAT CATCGGTGCG GGCCCGGCTT TCGACCTGAT CCTCACCGCG CGTGCGGTCG ACGCCACCGA AGCCCTACGG CTCGGGCTGG TTTCGCGCGT GTGCGCCGAC GTGACCGCCG AGGCCATGGA TATCGCTGAA ACGCTCTGTG GCTACGGCAA ATTCGGCGTC GAATCGACCA AACAGGTGCT GTGGGCGAAC CTGGATGCCG GATGCCTCGA GGCCGCGCTG CACGTCGAGA ACCGCAGTCA GATCCTGGCG TCGACCGAAG GCGGGGTACG CGCGTTCAGC GAGGAGTTCC GCCGCCGTCC ACGAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA