MysmA.00304.b

Cysteine desulfurase

CENTER ID: MysmA.00304.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00304.b.A1.GE28602 Full length( MysmA.00304.b ) 1 414
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00304.b.A1.PS00558 Full length( MysmA.00304.b ) 1 414
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_128800
PATRIC ID: fig|246196.19.peg.3086
RefSeq: YP_887440.1
UniProt: A0QX02
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAAKTLDVT AIRADFPILS RVMRGGNQLA YLDSGATSQK PLQVLDAERE FLLTSNGAVH RGAHQLMEES TDAYEQGRAD IAAFVGADPD ELVFTKNATE AINLVAYVLG DKRFERAVGP GDVIVTTEVE HHANLVPWQE LAERTGATLK WYGVTDSGRI DLDSLELDER VKVVAFSHHS NVTGAVAPVA ELVSRAKAVG ALTVLDACQS APHQPVDLHA LDVDYAAFSG HKMLGPTGIG VLYGRRGLLD AMPPFITGGS MIETVTMEKT TFAPAPQRFE AGTPMTSQVV GLAAAARYLT ALGMPAVEAH EAELVAAALE GLAGVPGVRI IGPTAMENRG SPVSFVVDGV HAHDVGQVLD DDGVAVRVGH HCAWPLHRRF GIAATARASF AVYNTLDEVD RLVAGVKRAV EFFA
NT Sequence
GTGACGGCCG CGAAGACGCT CGACGTGACG GCGATCAGGG CCGACTTCCC GATCCTGAGC CGGGTGATGC GCGGCGGAAA TCAGCTGGCG TATCTGGATT CCGGCGCGAC GTCGCAGAAA CCGCTGCAGG TGCTCGATGC CGAGCGTGAG TTCCTGCTGA CCTCCAACGG TGCCGTGCAC CGCGGCGCGC ACCAGCTGAT GGAGGAGTCC ACCGACGCCT ACGAGCAGGG ACGCGCCGAC ATCGCGGCCT TCGTGGGTGC CGACCCGGAT GAGCTGGTGT TCACCAAGAA CGCCACCGAG GCCATCAACC TGGTGGCATA CGTGCTGGGG GACAAGCGTT TCGAGCGGGC GGTCGGTCCC GGTGACGTCA TCGTCACCAC CGAGGTCGAG CACCACGCCA ACCTGGTTCC GTGGCAGGAA CTCGCCGAGC GTACGGGTGC GACGCTCAAG TGGTACGGCG TCACCGACTC CGGGCGCATC GACCTCGACT CGCTCGAACT CGACGAACGC GTGAAAGTCG TTGCGTTCAG CCATCACTCG AACGTCACGG GTGCCGTCGC GCCGGTCGCC GAACTGGTGT CGCGGGCCAA GGCGGTGGGT GCGCTGACCG TTCTGGACGC CTGCCAGTCG GCGCCGCACC AGCCGGTCGA CCTGCATGCG CTCGACGTCG ACTACGCGGC GTTCTCGGGG CACAAGATGC TGGGCCCCAC CGGCATCGGC GTGCTCTACG GCAGGCGGGG CCTGCTCGAC GCGATGCCGC CGTTCATCAC GGGCGGTTCG ATGATCGAGA CCGTGACGAT GGAGAAGACG ACCTTCGCGC CGGCGCCGCA GCGTTTCGAG GCAGGCACCC CGATGACCTC GCAGGTGGTC GGACTGGCTG CCGCGGCAAG GTATCTGACC GCTCTGGGCA TGCCTGCCGT CGAGGCGCAC GAGGCCGAAC TGGTCGCGGC GGCCCTCGAA GGGCTCGCCG GCGTACCCGG CGTGCGCATC ATCGGGCCGA CTGCGATGGA AAACCGCGGA TCCCCCGTGA GTTTCGTGGT CGACGGGGTC CATGCGCACG ACGTGGGCCA GGTCCTCGAC GACGACGGCG TCGCGGTACG CGTCGGGCAC CACTGCGCCT GGCCGCTGCA CCGCCGGTTC GGCATCGCGG CGACCGCGCG GGCATCGTTC GCGGTGTACA ACACCCTCGA CGAAGTGGAC CGCCTGGTGG CAGGCGTGAA ACGTGCTGTG GAGTTCTTTG CG
Details for MysmA.00304.b.A1.GE28602
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTAAKTLD VTAIRADFPI LSRVMRGGNQ LAYLDSGATS QKPLQVLDAE REFLLTSNGA VHRGAHQLME ESTDAYEQGR ADIAAFVGAD PDELVFTKNA TEAINLVAYV LGDKRFERAV GPGDVIVTTE VEHHANLVPW QELAERTGAT LKWYGVTDSG RIDLDSLELD ERVKVVAFSH HSNVTGAVAP VAELVSRAKA VGALTVLDAC QSAPHQPVDL HALDVDYAAF SGHKMLGPTG IGVLYGRRGL LDAMPPFITG GSMIETVTME KTTFAPAPQR FEAGTPMTSQ VVGLAAAARY LTALGMPAVE AHEAELVAAA LEGLAGVPGV RIIGPTAMEN RGSPVSFVVD GVHAHDVGQV LDDDGVAVRV GHHCAWPLHR RFGIAATARA SFAVYNTLDE VDRLVAG
Validated NT Sequence
ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatggt gacggccgcg aagacgctcg acgtgacggc gatcagggcc gacttcccga tcctgagccg ggtgatgcgc ggcggaaatc agctggcgta tctggattcc ggcgcgacgt cgcagaaacc gctgcaggtg ctcgatgccg agcgtgagtt cctgctgacc tccaacggtg ccgtgcaccg cggcgcgcac cagctgatgg aggagtccac cgacgcctac gagcagggac gcgccgacat cgcggccttc gtgggtgccg acccggatga gctggtgttc accaagaacg ccaccgaggc catcaacctg gtggcatacg tgctggggga caagcgtttc gagcgggcgg tcggtcccgg tgacgtcatc gtcaccaccg aggtcgagca ccacgccaac ctggttccgt ggcaggaact cgccgagcgt acgggtgcga cgctcaagtg gtacggcgtc accgactccg ggcgcatcga cctcgactcg ctcgaactcg acgaacgcgt gaaagtcgtt gcgttcagcc atcactcgaa cgtcacgggt gccgtcgcgc cggtcgccga actggtgtcg cgggccaagg cggtgggtgc gctgaccgtt ctggacgcct gccagtcggc gccgcaccag ccggtcgacc tgcatgcgct cgacgtcgac tacgcggcgt tctcggggca caagatgctg ggccccaccg gcatcggcgt gctctacggc aggcggggcc tgctcgacgc gatgccgccg ttcatcacgg gcggttcgat gatcgagacc gtgacgatgg agaagacgac cttcgcgccg gcgccgcagc gtttcgaggc aggcaccccg atgacctcgc aggtggtcgg actggctgcc gcggcaaggt atctgaccgc tctgggcatg cctgccgtcg aggcgcacga ggccgaactg gtcgcggcgg ccctcgaagg gctcgccggc gtacccggcg tgcgcatcat cgggccgact gcgatggaaa accgcggatc ccccgtgagt ttcgtggtcg acggggtcca tgcgcacgac gtgggccagg tcctcgacga cgacggcgtc gcggtacgcg tcgggcacca ctgcgcctgg ccgctgcacc gccggttcgg catcgcggcg accgcgcggg catcgttcgc ggtgtacaac accctcgacg aagtggaccg cctggtggca ggcg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTAAKTLD VTAIRADFPI LSRVMRGGNQ LAYLDSGATS QKPLQVLDAE REFLLTSNGA VHRGAHQLME ESTDAYEQGR ADIAAFVGAD PDELVFTKNA TEAINLVAYV LGDKRFERAV GPGDVIVTTE VEHHANLVPW QELAERTGAT LKWYGVTDSG RIDLDSLELD ERVKVVAFSH HSNVTGAVAP VAELVSRAKA VGALTVLDAC QSAPHQPVDL HALDVDYAAF SGHKMLGPTG IGVLYGRRGL LDAMPPFITG GSMIETVTME KTTFAPAPQR FEAGTPMTSQ VVGLAAAARY LTALGMPAVE AHEAELVAAA LEGLAGVPGV RIIGPTAMEN RGSPVSFVVD GVHAHDVGQV LDDDGVAVRV GHHCAWPLHR RFGIAATARA SFAVYNTLDE VDRLVAGVKR AVEFFA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacggcc gcgaagacgc tcgacgtgac ggcgatcagg gccgacttcc cgatcctgag ccgggtgatg cgcggcggaa atcagctggc gtatctggat tccggcgcga cgtcgcagaa accgctgcag gtgctcgatg ccgagcgtga gttcctgctg acctccaacg gtgccgtgca ccgcggcgcg caccagctga tggaggagtc caccgacgcc tacgagcagg gacgcgccga catcgcggcc ttcgtgggtg ccgacccgga tgagctggtg ttcaccaaga acgccaccga ggccatcaac ctggtggcat acgtgctggg ggacaagcgt ttcgagcggg cggtcggtcc cggtgacgtc atcgtcacca ccgaggtcga gcaccacgcc aacctggttc cgtggcagga actcgccgag cgtacgggtg cgacgctcaa gtggtacggc gtcaccgact ccgggcgcat cgacctcgac tcgctcgaac tcgacgaacg cgtgaaagtc gttgcgttca gccatcactc gaacgtcacg ggtgccgtcg cgccggtcgc cgaactggtg tcgcgggcca aggcggtggg tgcgctgacc gttctggacg cctgccagtc ggcgccgcac cagccggtcg acctgcatgc gctcgacgtc gactacgcgg cgttctcggg gcacaagatg ctgggcccca ccggcatcgg cgtgctctac ggcaggcggg gcctgctcga cgcgatgccg ccgttcatca cgggcggttc gatgatcgag accgtgacga tggagaagac gaccttcgcg ccggcgccgc agcgtttcga ggcaggcacc ccgatgacct cgcaggtggt cggactggct gccgcggcaa ggtatctgac cgctctgggc atgcctgccg tcgaggcgca cgaggccgaa ctggtcgcgg cggccctcga agggctcgcc ggcgtacccg gcgtgcgcat catcgggccg actgcgatgg aaaaccgcgg atcccccgtg agtttcgtgg tcgacggggt ccatgcgcac gacgtgggcc aggtcctcga cgacgacggc gtcgcggtac gcgtcgggca ccactgcgcc tggccgctgc accgccggtt cggcatcgcg gcgaccgcgc gggcatcgtt cgcggtgtac aacaccctcg acgaagtgga ccgcctggtg gcaggcgtga aacgtgctgt ggagttcttt gcgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MysmA.00304.b.A1.PS00558
PURIFICATION DATe: 4/6/2010
CONCENTRATION: 22.3mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTAAKTLD VTAIRADFPI LSRVMRGGNQ LAYLDSGATS QKPLQVLDAE REFLLTSNGA VHRGAHQLME ESTDAYEQGR ADIAAFVGAD PDELVFTKNA TEAINLVAYV LGDKRFERAV GPGDVIVTTE VEHHANLVPW QELAERTGAT LKWYGVTDSG RIDLDSLELD ERVKVVAFSH HSNVTGAVAP VAELVSRAKA VGALTVLDAC QSAPHQPVDL HALDVDYAAF SGHKMLGPTG IGVLYGRRGL LDAMPPFITG GSMIETVTME KTTFAPAPQR FEAGTPMTSQ VVGLAAAARY LTALGMPAVE AHEAELVAAA LEGLAGVPGV RIIGPTAMEN RGSPVSFVVD GVHAHDVGQV LDDDGVAVRV GHHCAWPLHR RFGIAATARA SFAVYNTLDE VDRLVAG
Validated NT Sequence
ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatggt gacggccgcg aagacgctcg acgtgacggc gatcagggcc gacttcccga tcctgagccg ggtgatgcgc ggcggaaatc agctggcgta tctggattcc ggcgcgacgt cgcagaaacc gctgcaggtg ctcgatgccg agcgtgagtt cctgctgacc tccaacggtg ccgtgcaccg cggcgcgcac cagctgatgg aggagtccac cgacgcctac gagcagggac gcgccgacat cgcggccttc gtgggtgccg acccggatga gctggtgttc accaagaacg ccaccgaggc catcaacctg gtggcatacg tgctggggga caagcgtttc gagcgggcgg tcggtcccgg tgacgtcatc gtcaccaccg aggtcgagca ccacgccaac ctggttccgt ggcaggaact cgccgagcgt acgggtgcga cgctcaagtg gtacggcgtc accgactccg ggcgcatcga cctcgactcg ctcgaactcg acgaacgcgt gaaagtcgtt gcgttcagcc atcactcgaa cgtcacgggt gccgtcgcgc cggtcgccga actggtgtcg cgggccaagg cggtgggtgc gctgaccgtt ctggacgcct gccagtcggc gccgcaccag ccggtcgacc tgcatgcgct cgacgtcgac tacgcggcgt tctcggggca caagatgctg ggccccaccg gcatcggcgt gctctacggc aggcggggcc tgctcgacgc gatgccgccg ttcatcacgg gcggttcgat gatcgagacc gtgacgatgg agaagacgac cttcgcgccg gcgccgcagc gtttcgaggc aggcaccccg atgacctcgc aggtggtcgg actggctgcc gcggcaaggt atctgaccgc tctgggcatg cctgccgtcg aggcgcacga ggccgaactg gtcgcggcgg ccctcgaagg gctcgccggc gtacccggcg tgcgcatcat cgggccgact gcgatggaaa accgcggatc ccccgtgagt ttcgtggtcg acggggtcca tgcgcacgac gtgggccagg tcctcgacga cgacggcgtc gcggtacgcg tcgggcacca ctgcgcctgg ccgctgcacc gccggttcgg catcgcggcg accgcgcggg catcgttcgc ggtgtacaac accctcgacg aagtggaccg cctggtggca ggcg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTAAKTLD VTAIRADFPI LSRVMRGGNQ LAYLDSGATS QKPLQVLDAE REFLLTSNGA VHRGAHQLME ESTDAYEQGR ADIAAFVGAD PDELVFTKNA TEAINLVAYV LGDKRFERAV GPGDVIVTTE VEHHANLVPW QELAERTGAT LKWYGVTDSG RIDLDSLELD ERVKVVAFSH HSNVTGAVAP VAELVSRAKA VGALTVLDAC QSAPHQPVDL HALDVDYAAF SGHKMLGPTG IGVLYGRRGL LDAMPPFITG GSMIETVTME KTTFAPAPQR FEAGTPMTSQ VVGLAAAARY LTALGMPAVE AHEAELVAAA LEGLAGVPGV RIIGPTAMEN RGSPVSFVVD GVHAHDVGQV LDDDGVAVRV GHHCAWPLHR RFGIAATARA SFAVYNTLDE VDRLVAGVKR AVEFFA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACGGCC GCGAAGACGC TCGACGTGAC GGCGATCAGG GCCGACTTCC CGATCCTGAG CCGGGTGATG CGCGGCGGAA ATCAGCTGGC GTATCTGGAT TCCGGCGCGA CGTCGCAGAA ACCGCTGCAG GTGCTCGATG CCGAGCGTGA GTTCCTGCTG ACCTCCAACG GTGCCGTGCA CCGCGGCGCG CACCAGCTGA TGGAGGAGTC CACCGACGCC TACGAGCAGG GACGCGCCGA CATCGCGGCC TTCGTGGGTG CCGACCCGGA TGAGCTGGTG TTCACCAAGA ACGCCACCGA GGCCATCAAC CTGGTGGCAT ACGTGCTGGG GGACAAGCGT TTCGAGCGGG CGGTCGGTCC CGGTGACGTC ATCGTCACCA CCGAGGTCGA GCACCACGCC AACCTGGTTC CGTGGCAGGA ACTCGCCGAG CGTACGGGTG CGACGCTCAA GTGGTACGGC GTCACCGACT CCGGGCGCAT CGACCTCGAC TCGCTCGAAC TCGACGAACG CGTGAAAGTC GTTGCGTTCA GCCATCACTC GAACGTCACG GGTGCCGTCG CGCCGGTCGC CGAACTGGTG TCGCGGGCCA AGGCGGTGGG TGCGCTGACC GTTCTGGACG CCTGCCAGTC GGCGCCGCAC CAGCCGGTCG ACCTGCATGC GCTCGACGTC GACTACGCGG CGTTCTCGGG GCACAAGATG CTGGGCCCCA CCGGCATCGG CGTGCTCTAC GGCAGGCGGG GCCTGCTCGA CGCGATGCCG CCGTTCATCA CGGGCGGTTC GATGATCGAG ACCGTGACGA TGGAGAAGAC GACCTTCGCG CCGGCGCCGC AGCGTTTCGA GGCAGGCACC CCGATGACCT CGCAGGTGGT CGGACTGGCT GCCGCGGCAA GGTATCTGAC CGCTCTGGGC ATGCCTGCCG TCGAGGCGCA CGAGGCCGAA CTGGTCGCGG CGGCCCTCGA AGGGCTCGCC GGCGTACCCG GCGTGCGCAT CATCGGGCCG ACTGCGATGG AAAACCGCGG ATCCCCCGTG AGTTTCGTGG TCGACGGGGT CCATGCGCAC GACGTGGGCC AGGTCCTCGA CGACGACGGC GTCGCGGTAC GCGTCGGGCA CCACTGCGCC TGGCCGCTGC ACCGCCGGTT CGGCATCGCG GCGACCGCGC GGGCATCGTT CGCGGTGTAC AACACCCTCG ACGAAGTGGA CCGCCTGGTG GCAGGCGTGA AACGTGCTGT GGAGTTCTTT GCGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA