MysmA.00304.a

Pyridoxal-phosphate-dependent transferase

CENTER ID: MysmA.00304.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00304.a.A1.GE28601 Full length( MysmA.00304.a ) 1 371
MysmA.00304.a.AE1.GE43155 Full length( MysmA.00304.a ) 1 371
MysmA.00304.a.B1.GE43078 Full length( MysmA.00304.a ) 1 371
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00304.a.A1.PS00556 Full length( MysmA.00304.a ) 1 371
MysmA.00304.a.AE1.PW38687 Full length( MysmA.00304.a ) 1 371

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.6085
RefSeq: YP_890465.1
UniProt: A0R5M7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMLAQQWRDA RPKVAGLHLD SGACSRQSFA VIDATTAHAR HEAEVGGYVA AEAATPALDA GRAAVASLIG FAASDVVYTS GSNHAIDLLL SSWPGKRTLA CLPGEYGPNL SAMAANGFQV RALPVDDDGR VLVDEASHEL SAHPVALVHL TALASHRGIA QPAAELVEAC HNAGIPVVID AAQALGHLDC NVGADAVYSS SRKWLAGPRG VGVLAVRPEL AERLQPRIPP SDWPIPMSVL EKLELGEHNA AARVGFSVAV GEHLAAGPTA VRERLAEVGR LSRQVLAEVD GWRVVEPVDQ PTAITTLEST DGADPASVRS WLIAERGIVT TACELARAPF EMRTPVLRIS PHVDVTVDEL EQFAAALREA P
NT Sequence
GTGATGCTCG CGCAGCAGTG GCGTGACGCC CGTCCCAAGG TTGCCGGGTT GCACCTGGAC AGCGGGGCAT GTTCGCGGCA GAGCTTCGCG GTGATCGACG CGACCACCGC ACACGCACGC CACGAGGCCG AGGTGGGTGG TTATGTGGCG GCCGAGGCTG CGACGCCGGC GCTCGACGCC GGGCGGGCCG CGGTCGCGTC GCTCATCGGT TTTGCGGCGT CGGACGTGGT GTACACCAGC GGATCCAACC ACGCCATCGA CCTGTTGCTG TCGAGCTGGC CGGGGAAGCG CACGCTGGCC TGCCTGCCCG GCGAGTACGG GCCGAATCTG TCTGCCATGG CGGCCAACGG TTTCCAGGTG CGTGCGCTAC CGGTCGACGA CGACGGGCGG GTGCTGGTCG ACGAGGCGTC GCACGAACTG TCGGCCCATC CCGTCGCGCT CGTACACCTC ACCGCATTGG CAAGCCATCG CGGGATCGCG CAACCCGCGG CAGAACTCGT CGAGGCCTGC CACAATGCGG GGATCCCCGT GGTGATCGAC GCCGCGCAGG CGCTGGGGCA TCTGGACTGC AATGTCGGGG CCGACGCGGT GTACTCATCG TCGCGCAAGT GGCTCGCCGG CCCGCGTGGT GTCGGGGTGC TCGCGGTGCG GCCCGAACTC GCCGAGCGTC TGCAACCGCG GATCCCCCCG TCCGACTGGC CAATTCCGAT GAGCGTCTTG GAGAAGCTCG AACTAGGTGA GCACAACGCG GCGGCGCGTG TGGGATTCTC CGTCGCGGTT GGTGAGCATC TCGCAGCAGG GCCCACGGCG GTGCGCGAAC GACTCGCCGA GGTGGGGCGT CTCTCTCGGC AGGTGCTGGC AGAGGTCGAC GGGTGGCGCG TCGTCGAACC CGTCGACCAA CCCACCGCGA TCACCACCCT TGAGTCCACC GATGGTGCCG ATCCCGCGTC GGTGCGCTCG TGGCTGATCG CGGAGCGTGG CATCGTGACC ACCGCGTGTG AACTCGCGCG GGCACCGTTC GAGATGCGCA CGCCGGTGCT GCGAATCTCG CCGCACGTCG ACGTGACGGT CGACGAACTG GAGCAGTTCG CCGCAGCGTT GCGTGAGGCG CCC
Details for MysmA.00304.a.A1.GE28601
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVMLAQQWR DARPKVAGLH LDSGACSRQS FAVIDATTAH ARHEAEVGGY VAAEAATPAL DAGRAAVASL IGFAASDVVY TSGSNHAIDL LLSSWPGKRT LACLPGEYGP NLSAMAANGF QVRALPVDDD GRVLVDEASH ELSAHPVALV HLTALASHRG IAQPAAELVE ACHNAGIPVV IDAAQALGHL DCNVGADAVY SSSRKWLAGP RGVGVLAVRP ELAERLQPRI PPSDWPIPMS VLEKLELGEH NAAARVGFSV AVGEHLAAGP TAVRERLAEV GRLSRQVLAE VDGWRVVEPV DQPTAITTLE STDGADPASV RSWLIAERGI VTTACELARA PFEMRTPVLR ISPHVDVTVD ELEQFA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga tgctcgcgca gcagtggcgt gacgcccgtc ccaaggttgc cgggttgcac ctggacagcg gggcatgttc gcggcagagc ttcgcggtga tcgacgcgac caccgcacac gcacgccacg aggccgaggt gggtggttat gtggcggccg aggctgcgac gccggcgctc gacgccgggc gggccgcggt cgcgtcgctc atcggttttg cggcgtcgga cgtggtgtac accagcggat ccaaccacgc catcgacctg ttgctgtcga gctggccggg gaagcgcacg ctggcctgcc tgcccggcga gtacgggccg aatctgtctg ccatggcggc caacggtttc caggtgcgtg cgctaccggt cgacgacgac gggcgggtgc tggtcgacga ggcgtcgcac gaactgtcgg cccatcccgt cgcgctcgta cacctcaccg cattggcaag ccatcgcggg atcgcgcaac ccgcggcaga actcgtcgag gcctgccaca atgcggggat ccccgtggtg atcgacgccg cgcaggcgct ggggcatctg gactgcaatg tcggggccga cgcggtgtac tcatcgtcgc gcaagtggct cgccggcccg cgtggtgtcg gggtgctcgc ggtgcggccc gaactcgccg agcgtctgca accgcggatc cccccgtccg actggccaat tccgatgagc gtcttggaga agctcgaact aggtgagcac aacgcggcgg cgcgtgtggg attctccgtc gcggttggtg agcatctcgc agcagggccc acggcggtgc gcgaacgact cgccgaggtg gggcgtctct ctcggcaggt gctggcagag gtcgacgggt ggcgcgtcgt cgaacccgtc gaccaaccca ccgcgatcac cacccttgag tccaccgatg gtgccgatcc cgcgtcggtg cgctcgtggc tgatcgcgga gcgtggcatc gtgaccaccg cgtgtgaact cgcgcgggca ccgttcgaga tgcgcacgcc ggtgctgcga atctcgccgc acgtcgacgt gacggtcgac gaactggagc agttcgccgc a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVMLAQQWR DARPKVAGLH LDSGACSRQS FAVIDATTAH ARHEAEVGGY VAAEAATPAL DAGRAAVASL IGFAASDVVY TSGSNHAIDL LLSSWPGKRT LACLPGEYGP NLSAMAANGF QVRALPVDDD GRVLVDEASH ELSAHPVALV HLTALASHRG IAQPAAELVE ACHNAGIPVV IDAAQALGHL DCNVGADAVY SSSRKWLAGP RGVGVLAVRP ELAERLQPRI PPSDWPIPMS VLEKLELGEH NAAARVGFSV AVGEHLAAGP TAVRERLAEV GRLSRQVLAE VDGWRVVEPV DQPTAITTLE STDGADPASV RSWLIAERGI VTTACELARA PFEMRTPVLR ISPHVDVTVD ELEQFAAALR EAP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgatgctc gcgcagcagt ggcgtgacgc ccgtcccaag gttgccgggt tgcacctgga cagcggggca tgttcgcggc agagcttcgc ggtgatcgac gcgaccaccg cacacgcacg ccacgaggcc gaggtgggtg gttatgtggc ggccgaggct gcgacgccgg cgctcgacgc cgggcgggcc gcggtcgcgt cgctcatcgg ttttgcggcg tcggacgtgg tgtacaccag cggatccaac cacgccatcg acctgttgct gtcgagctgg ccggggaagc gcacgctggc ctgcctgccc ggcgagtacg ggccgaatct gtctgccatg gcggccaacg gtttccaggt gcgtgcgcta ccggtcgacg acgacgggcg ggtgctggtc gacgaggcgt cgcacgaact gtcggcccat cccgtcgcgc tcgtacacct caccgcattg gcaagccatc gcgggatcgc gcaacccgcg gcagaactcg tcgaggcctg ccacaatgcg gggatccccg tggtgatcga cgccgcgcag gcgctggggc atctggactg caatgtcggg gccgacgcgg tgtactcatc gtcgcgcaag tggctcgccg gcccgcgtgg tgtcggggtg ctcgcggtgc ggcccgaact cgccgagcgt ctgcaaccgc ggatcccccc gtccgactgg ccaattccga tgagcgtctt ggagaagctc gaactaggtg agcacaacgc ggcggcgcgt gtgggattct ccgtcgcggt tggtgagcat ctcgcagcag ggcccacggc ggtgcgcgaa cgactcgccg aggtggggcg tctctctcgg caggtgctgg cagaggtcga cgggtggcgc gtcgtcgaac ccgtcgacca acccaccgcg atcaccaccc ttgagtccac cgatggtgcc gatcccgcgt cggtgcgctc gtggctgatc gcggagcgtg gcatcgtgac caccgcgtgt gaactcgcgc gggcaccgtt cgagatgcgc acgccggtgc tgcgaatctc gccgcacgtc gacgtgacgg tcgacgaact ggagcagttc gccgcagcgt tgcgtgaggc gcccaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MysmA.00304.a.AE1.GE43155
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MMLAQQWRDA RPKVAGLHLD SGACSRQSFA VIDATTAHAR HEAEVGGYVA AEAATPALDA GRAAVASLIG FAASDVVYTS GSNHAIDLLL SSWPGKRTLA CLPGEYGPNL SAMAANGFQV RALPVDDDGR VLVDEASHEL SAHPVALVHL TALASHRGIA QPAAELVEAC HNAGIPVVID AAQALGHLDC NVGADAVYSS SRKWLAGPRG VGVLAVRPEL AERLQPRIPP SDWPIPMSVL EKLELGEHNA AARVGFSVAV GEHLAAGPTA VRERLAEVGR LSRQVLAEVD GWRVVEPVDQ PTAITTLEST DGADPASVRS WLIAERGIVT TACELARAPF EMRTPVLRIS PHVDVTVDEL EQFAAALREA PGHHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccgt gatgctcgcg cagcagtggc gtgacgcccg tcccaaggtt gccgggttgc acctggacag cggggcatgt tcgcggcaga gcttcgcggt gatcgacgcg accaccgcac acgcacgcca cgaggccgag gtgggtggtt atgtggcggc cgaggctgcg acgccggcgc tcgacgccgg gcgggccgcg gtcgcgtcgc tcatcggttt tgcggcgtcg gacgtggtgt acaccagcgg atccaaccac gccatcgacc tgttgctgtc gagctggccg gggaagcgca cgctggcctg cctgcccggc gagtacgggc cgaatctgtc tgccatggcg gccaacggtt tccaggtgcg tgcgctaccg gtcgacgacg acgggcgggt gctggtcgac gaggcgtcgc acgaactgtc ggcccatccc gtcgcgctcg tacacctcac cgcattggca agccatcgcg ggatcgcgca acccgcggca gaactcgtcg aggcctgcca caatgcgggg atccccgtgg tgatcgacgc cgcgcaggcg ctggggcatc tggactgcaa tgtcggggcc gacgcggtgt actcatcgtc gcgcaagtgg ctcgccggcc cgcgtggtgt cggggtgctc gcggtgcggc ccgaactcgc cgagcgtctg caaccgcgga tccccccgtc cgactggcca attccgatga gcgtcttgga gaagctcgaa ctaggtgagc acaacgcggc ggcgcgtgtg ggattctccg tcgcggttgg tgagcatctc gcagcagggc ccacggcggt gcgcgaacga ctcgccgagg tggggcgtct ctctcggcag gtgctggcag aggtcgacgg gtggcgcgtc gtcgaacccg tcgaccaacc caccgcgatc accacccttg agtccaccga tggtgccgat cccgcgtcgg tgcgctcgtg gctgatcgcg gagcgtggca tcgtgaccac cgcgtgtgaa ctcgcgcggg caccgttcga gatgcgcacg ccggtgctgc gaatctcgcc gcacgtcgac gtgacggtcg acgaactgga gcagttcgcc gcagcgttgc gtgaggcgcc cgggcaccac catcatcatc attaacggat ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat
Details for MysmA.00304.a.B1.GE43078
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMM LAQQWRDARP KVAGLHLDSG ACSRQSFAVI DATTAHARHE AEVGGYVAAE AATPALDAGR AAVASLIGFA ASDVVYTSGS NHAIDLLLSS WPGKRTLACL PGEYGPNLSA MAANGFQVRA LPVDDDGRVL VDEASHELSA HPVALVHLTA LASHRGIAQP AAELVEACHN AGIPVVIDAA QALGHLDCNV GADAVYSSSR KWLAGPRGVG VLAVRPELAE RLQPRIPPSD WPIPMSVLEK LELGEHNAAA RVGFSVAVGE HLAAGPTAVR ERLAEVGRLS RQVLAEVDGW RVVEPVDQPT AITTLESTDG ADPASVRSWL IAERGIVTTA CELARAPFEM RTPVLRISPH VDVTVDELEQ FAAALREAP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgatgc tcgcgcagca gtggcgtgac gcccgtccca aggttgccgg gttgcacctg gacagcgggg catgttcgcg gcagagcttc gcggtgatcg acgcgaccac cgcacacgca cgccacgagg ccgaggtggg tggttatgtg gcggccgagg ctgcgacgcc ggcgctcgac gccgggcggg ccgcggtcgc gtcgctcatc ggttttgcgg cgtcggacgt ggtgtacacc agcggatcca accacgccat cgacctgttg ctgtcgagct ggccggggaa gcgcacgctg gcctgcctgc ccggcgagta cgggccgaat ctgtctgcca tggcggccaa cggtttccag gtgcgtgcgc taccggtcga cgacgacggg cgggtgctgg tcgacgaggc gtcgcacgaa ctgtcggccc atcccgtcgc gctcgtacac ctcaccgcat tggcaagcca tcgcgggatc gcgcaacccg cggcagaact cgtcgaggcc tgccacaatg cggggatccc cgtggtgatc gacgccgcgc aggcgctggg gcatctggac tgcaatgtcg gggccgacgc ggtgtactca tcgtcgcgca agtggctcgc cggcccgcgt ggtgtcgggg tgctcgcggt gcggcccgaa ctcgccgagc gtctgcaacc gcggatcccc ccgtccgact ggccaattcc gatgagcgtc ttggagaagc tcgaactagg tgagcacaac gcggcggcgc gtgtgggatt ctccgtcgcg gttggtgagc atctcgcagc agggcccacg gcggtgcgcg aacgactcgc cgaggtgggg cgtctctctc ggcaggtgct ggcagaggtc gacgggtggc gcgtcgtcga acccgtcgac caacccaccg cgatcaccac ccttgagtcc accgatggtg ccgatcccgc gtcggtgcgc tcgtggctga tcgcggagcg tggcatcgtg accaccgcgt gtgaactcgc gcgggcaccg ttcgagatgc gcacgccggt gctgcgaatc tcgccgcacg tcgacgtgac ggtcgacgaa ctggagcagt tcgccgcagc gttgcgtgag gcgccctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for MysmA.00304.a.A1.PS00556
PURIFICATION DATe: 4/8/2010
CONCENTRATION: 17.8mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVMLAQQWR DARPKVAGLH LDSGACSRQS FAVIDATTAH ARHEAEVGGY VAAEAATPAL DAGRAAVASL IGFAASDVVY TSGSNHAIDL LLSSWPGKRT LACLPGEYGP NLSAMAANGF QVRALPVDDD GRVLVDEASH ELSAHPVALV HLTALASHRG IAQPAAELVE ACHNAGIPVV IDAAQALGHL DCNVGADAVY SSSRKWLAGP RGVGVLAVRP ELAERLQPRI PPSDWPIPMS VLEKLELGEH NAAARVGFSV AVGEHLAAGP TAVRERLAEV GRLSRQVLAE VDGWRVVEPV DQPTAITTLE STDGADPASV RSWLIAERGI VTTACELARA PFEMRTPVLR ISPHVDVTVD ELEQFA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga tgctcgcgca gcagtggcgt gacgcccgtc ccaaggttgc cgggttgcac ctggacagcg gggcatgttc gcggcagagc ttcgcggtga tcgacgcgac caccgcacac gcacgccacg aggccgaggt gggtggttat gtggcggccg aggctgcgac gccggcgctc gacgccgggc gggccgcggt cgcgtcgctc atcggttttg cggcgtcgga cgtggtgtac accagcggat ccaaccacgc catcgacctg ttgctgtcga gctggccggg gaagcgcacg ctggcctgcc tgcccggcga gtacgggccg aatctgtctg ccatggcggc caacggtttc caggtgcgtg cgctaccggt cgacgacgac gggcgggtgc tggtcgacga ggcgtcgcac gaactgtcgg cccatcccgt cgcgctcgta cacctcaccg cattggcaag ccatcgcggg atcgcgcaac ccgcggcaga actcgtcgag gcctgccaca atgcggggat ccccgtggtg atcgacgccg cgcaggcgct ggggcatctg gactgcaatg tcggggccga cgcggtgtac tcatcgtcgc gcaagtggct cgccggcccg cgtggtgtcg gggtgctcgc ggtgcggccc gaactcgccg agcgtctgca accgcggatc cccccgtccg actggccaat tccgatgagc gtcttggaga agctcgaact aggtgagcac aacgcggcgg cgcgtgtggg attctccgtc gcggttggtg agcatctcgc agcagggccc acggcggtgc gcgaacgact cgccgaggtg gggcgtctct ctcggcaggt gctggcagag gtcgacgggt ggcgcgtcgt cgaacccgtc gaccaaccca ccgcgatcac cacccttgag tccaccgatg gtgccgatcc cgcgtcggtg cgctcgtggc tgatcgcgga gcgtggcatc gtgaccaccg cgtgtgaact cgcgcgggca ccgttcgaga tgcgcacgcc ggtgctgcga atctcgccgc acgtcgacgt gacggtcgac gaactggagc agttcgccgc a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVMLAQQWR DARPKVAGLH LDSGACSRQS FAVIDATTAH ARHEAEVGGY VAAEAATPAL DAGRAAVASL IGFAASDVVY TSGSNHAIDL LLSSWPGKRT LACLPGEYGP NLSAMAANGF QVRALPVDDD GRVLVDEASH ELSAHPVALV HLTALASHRG IAQPAAELVE ACHNAGIPVV IDAAQALGHL DCNVGADAVY SSSRKWLAGP RGVGVLAVRP ELAERLQPRI PPSDWPIPMS VLEKLELGEH NAAARVGFSV AVGEHLAAGP TAVRERLAEV GRLSRQVLAE VDGWRVVEPV DQPTAITTLE STDGADPASV RSWLIAERGI VTTACELARA PFEMRTPVLR ISPHVDVTVD ELEQFAAALR EAP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGATGCTC GCGCAGCAGT GGCGTGACGC CCGTCCCAAG GTTGCCGGGT TGCACCTGGA CAGCGGGGCA TGTTCGCGGC AGAGCTTCGC GGTGATCGAC GCGACCACCG CACACGCACG CCACGAGGCC GAGGTGGGTG GTTATGTGGC GGCCGAGGCT GCGACGCCGG CGCTCGACGC CGGGCGGGCC GCGGTCGCGT CGCTCATCGG TTTTGCGGCG TCGGACGTGG TGTACACCAG CGGATCCAAC CACGCCATCG ACCTGTTGCT GTCGAGCTGG CCGGGGAAGC GCACGCTGGC CTGCCTGCCC GGCGAGTACG GGCCGAATCT GTCTGCCATG GCGGCCAACG GTTTCCAGGT GCGTGCGCTA CCGGTCGACG ACGACGGGCG GGTGCTGGTC GACGAGGCGT CGCACGAACT GTCGGCCCAT CCCGTCGCGC TCGTACACCT CACCGCATTG GCAAGCCATC GCGGGATCGC GCAACCCGCG GCAGAACTCG TCGAGGCCTG CCACAATGCG GGGATCCCCG TGGTGATCGA CGCCGCGCAG GCGCTGGGGC ATCTGGACTG CAATGTCGGG GCCGACGCGG TGTACTCATC GTCGCGCAAG TGGCTCGCCG GCCCGCGTGG TGTCGGGGTG CTCGCGGTGC GGCCCGAACT CGCCGAGCGT CTGCAACCGC GGATCCCCCC GTCCGACTGG CCAATTCCGA TGAGCGTCTT GGAGAAGCTC GAACTAGGTG AGCACAACGC GGCGGCGCGT GTGGGATTCT CCGTCGCGGT TGGTGAGCAT CTCGCAGCAG GGCCCACGGC GGTGCGCGAA CGACTCGCCG AGGTGGGGCG TCTCTCTCGG CAGGTGCTGG CAGAGGTCGA CGGGTGGCGC GTCGTCGAAC CCGTCGACCA ACCCACCGCG ATCACCACCC TTGAGTCCAC CGATGGTGCC GATCCCGCGT CGGTGCGCTC GTGGCTGATC GCGGAGCGTG GCATCGTGAC CACCGCGTGT GAACTCGCGC GGGCACCGTT CGAGATGCGC ACGCCGGTGC TGCGAATCTC GCCGCACGTC GACGTGACGG TCGACGAACT GGAGCAGTTC GCCGCAGCGT TGCGTGAGGC GCCCAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for MysmA.00304.a.AE1.PW38687
PURIFICATION DATe: 8/14/2019
CONCENTRATION: 36.2mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: MMLAQQWRDA RPKVAGLHLD SGACSRQSFA VIDATTAHAR HEAEVGGYVA AEAATPALDA GRAAVASLIG FAASDVVYTS GSNHAIDLLL SSWPGKRTLA CLPGEYGPNL SAMAANGFQV RALPVDDDGR VLVDEASHEL SAHPVALVHL TALASHRGIA QPAAELVEAC HNAGIPVVID AAQALGHLDC NVGADAVYSS SRKWLAGPRG VGVLAVRPEL AERLQPRIPP SDWPIPMSVL EKLELGEHNA AARVGFSVAV GEHLAAGPTA VRERLAEVGR LSRQVLAEVD GWRVVEPVDQ PTAITTLEST DGADPASVRS WLIAERGIVT TACELARAPF EMRTPVLRIS PHVDVTVDEL
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MMLAQQWRDA RPKVAGLHLD SGACSRQSFA VIDATTAHAR HEAEVGGYVA AEAATPALDA GRAAVASLIG FAASDVVYTS GSNHAIDLLL SSWPGKRTLA CLPGEYGPNL SAMAANGFQV RALPVDDDGR VLVDEASHEL SAHPVALVHL TALASHRGIA QPAAELVEAC HNAGIPVVID AAQALGHLDC NVGADAVYSS SRKWLAGPRG VGVLAVRPEL AERLQPRIPP SDWPIPMSVL EKLELGEHNA AARVGFSVAV GEHLAAGPTA VRERLAEVGR LSRQVLAEVD GWRVVEPVDQ PTAITTLEST DGADPASVRS WLIAERGIVT TACELARAPF EMRTPVLRIS PHVDVTVDEL EQFAAALREA PGHHHHHH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCGT GATGCTCGCG CAGCAGTGGC GTGACGCCCG TCCCAAGGTT GCCGGGTTGC ACCTGGACAG CGGGGCATGT TCGCGGCAGA GCTTCGCGGT GATCGACGCG ACCACCGCAC ACGCACGCCA CGAGGCCGAG GTGGGTGGTT ATGTGGCGGC CGAGGCTGCG ACGCCGGCGC TCGACGCCGG GCGGGCCGCG GTCGCGTCGC TCATCGGTTT TGCGGCGTCG GACGTGGTGT ACACCAGCGG ATCCAACCAC GCCATCGACC TGTTGCTGTC GAGCTGGCCG GGGAAGCGCA CGCTGGCCTG CCTGCCCGGC GAGTACGGGC CGAATCTGTC TGCCATGGCG GCCAACGGTT TCCAGGTGCG TGCGCTACCG GTCGACGACG ACGGGCGGGT GCTGGTCGAC GAGGCGTCGC ACGAACTGTC GGCCCATCCC GTCGCGCTCG TACACCTCAC CGCATTGGCA AGCCATCGCG GGATCGCGCA ACCCGCGGCA GAACTCGTCG AGGCCTGCCA CAATGCGGGG ATCCCCGTGG TGATCGACGC CGCGCAGGCG CTGGGGCATC TGGACTGCAA TGTCGGGGCC GACGCGGTGT ACTCATCGTC GCGCAAGTGG CTCGCCGGCC CGCGTGGTGT CGGGGTGCTC GCGGTGCGGC CCGAACTCGC CGAGCGTCTG CAACCGCGGA TCCCCCCGTC CGACTGGCCA ATTCCGATGA GCGTCTTGGA GAAGCTCGAA CTAGGTGAGC ACAACGCGGC GGCGCGTGTG GGATTCTCCG TCGCGGTTGG TGAGCATCTC GCAGCAGGGC CCACGGCGGT GCGCGAACGA CTCGCCGAGG TGGGGCGTCT CTCTCGGCAG GTGCTGGCAG AGGTCGACGG GTGGCGCGTC GTCGAACCCG TCGACCAACC CACCGCGATC ACCACCCTTG AGTCCACCGA TGGTGCCGAT CCCGCGTCGG TGCGCTCGTG GCTGATCGCG GAGCGTGGCA TCGTGACCAC CGCGTGTGAA CTCGCGCGGG CACCGTTCGA GATGCGCACG CCGGTGCTGC GAATCTCGCC GCACGTCGAC GTGACGGTCG ACGAACTGGA GCAGTTCGCC GCAGCGTTGC GTGAGGCGCC CGGGCACCAC CATCATCATC ATTAACGGAT CCGAATTCGA GCTCCGTCGA CAAGCTTGCG GCCGCACTCG AGCACCACCA CCACCACCAC TGAGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGAT