SSGCID: Target Page

MysmA.00295.a

Oxidoreductase, zinc-binding dehydrogenase family protein

CENTER ID:	MysmA.00295.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00295.a.A1.GE28600	Full length( MysmA.00295.a )		1	363

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.124
RefSeq:	YP_884543.1
UniProt:	A0QNQ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKIRGAVLER IGAPRPYAES RPLTISELDL ADPGPDELLV RIESAGLCHS DLSVVDGNRV RPVPMLLGHE AAGVVETPGA DLETGQRVVM TFLPRCGSCP ACGTDGLTPC GPGSVANGAG TLMNGDIRLR RDGEPVFHHL GVSGFATHAV VDRRSIVPVP DDVPATVAAL LGCAVLTGGG AVLNVGRPKP GQTVVVVGLG GVGMAAALTA LAHDDVRVVG VDQLSDKLAR AAELGVHETF TPEQAADLRA EVVIEAAGHP AALETAIGLT GPGGRTITVG LPRPDARITV SPLGFVAEGR SLIGSYLGSA VPSRDIPRFV ELWRAGRLPV ESLVSSTIAL DDINAGMDEL AEGRAVRQVI DFA

NT Sequence

ATGAAGATCC GCGGAGCCGT CCTCGAACGC ATCGGTGCAC CCCGTCCCTA CGCCGAATCC CGGCCACTGA CCATCTCCGA ACTCGACCTC GCCGATCCGG GTCCGGATGA ACTGCTGGTG CGCATCGAGT CCGCCGGGCT GTGCCACTCG GATCTGTCGG TCGTCGACGG CAACCGGGTG CGTCCGGTGC CGATGCTGCT GGGGCACGAG GCCGCGGGTG TGGTCGAGAC GCCGGGCGCG GATCTCGAAA CCGGTCAGCG CGTGGTGATG ACGTTCCTGC CGCGCTGCGG ATCCTGCCCG GCCTGCGGGA CCGACGGGCT GACCCCCTGC GGGCCGGGAT CGGTCGCCAA CGGCGCGGGC ACGCTGATGA ACGGTGACAT CCGGCTGCGT CGCGACGGCG AACCTGTGTT CCACCACCTC GGTGTGTCCG GGTTCGCGAC GCACGCCGTG GTGGACCGCC GCTCGATCGT GCCGGTACCC GACGACGTGC CCGCGACGGT CGCCGCGCTG TTGGGTTGCG CGGTGCTCAC GGGCGGCGGG GCCGTGCTCA ACGTCGGCCG GCCGAAGCCG GGACAGACCG TCGTGGTCGT CGGTCTCGGC GGGGTGGGGA TGGCCGCTGC GCTCACCGCG CTCGCGCACG ACGACGTGCG GGTGGTCGGT GTCGACCAGT TGTCCGACAA GCTGGCCCGC GCAGCCGAAC TCGGCGTGCA CGAGACCTTC ACCCCCGAGC AGGCCGCGGA TCTGCGGGCC GAGGTGGTGA TCGAGGCCGC GGGACATCCG GCCGCGCTGG AGACCGCGAT CGGTTTGACC GGTCCTGGCG GACGGACCAT CACGGTGGGC CTGCCGCGCC CCGACGCGCG GATCACGGTG TCGCCGTTGG GTTTCGTCGC CGAAGGACGG TCTCTGATCG GCAGTTACCT CGGTTCGGCG GTGCCGTCGC GCGACATCCC GCGTTTCGTC GAGTTGTGGC GCGCCGGGCG CCTGCCGGTG GAGTCGCTCG TGTCGTCGAC CATCGCGCTC GACGACATCA ACGCGGGCAT GGACGAACTG GCCGAGGGCC GTGCGGTGCG TCAGGTGATC GACTTCGCC

Details for MysmA.00295.a.A1.GE28600

HARVESTED ON:	2/17/2010
SEQUENCED ON:	2/24/2010
EXPECTED MW:	39kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

attaggcgaa gtcgatcacc tgacgcaccg cacggccctc ggccagttcg tccatgcccg cgttgatgtc gtcgagcgcg atggtcgacg acacgagcga ctccaccggc aggcgcccgg cgcgccacaa ctcgacgaaa cgcgggatgt cgcgcgacgg caccgccgaa ccgaggtaac tgccgatcag agaccgtcct tcggcgacga aacccaacgg cgacaccgtg atccgcgcgt cggggcgcgg caggcccacc gtgatggtcc gtccgccagg accggtcaaa ccgatcgcgg tctccagcgc ggccggatgt cccgcggcct cgatcaccac ctcggcccgc agatccgcgg cctgctcggg ggtgaaggtc tcgtgcacgc cgagttcggc tgcgcgggcc agcttgtcgg acaactggtc gacaccgacc acccgcacgt cgtcgtgcgc gagcgcggtg agcgcagcgg ccatccccac cccgccgaga ccgacgacca cgacggtctg tcccggcttc ggccggccga cgttgagcac ggccccgccg cccgtgagca ccgcgcaacc caacagcgcg gcgaccgtcg cgggcacgtc gtcgggtacc ggcacgatcg agcggcggtc caccacggcg tgcgtcgcga acccggacac accgaggtgg tggaacacag gttcgccgtc gcgacgcagc cggatgtcac cgttcatcag cgtgcccgcg ccgttggcga ccgatcccgg cccgcagggg gtcagcccgt cggtcccgca ggccgggcag gatccgcagc gcggcaggaa cgtcatcacc acgcgctgac cggtttcgag atccgcgccc ggcgtctcga ccacacccgc ggcctcgtgc cccagcagca tcggcaccgg acgcacccgg ttgccgtcga cgaccgacag atccgagtgg cacagcccgg cggactcgat gcgcaccagc agttcatccg gacccggatc ggcgaggtcg agttcggaga tggtcagtgg ccgggattcg gcgtagggac ggggtgcacc gatgcgttcg aggacggctc cgcggatctt catcgaacca ggaccctggg tctgagcttc cagggt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKIRGAVLE RIGAPRPYAE SRPLTISELD LADPGPDELL VRIESAGLCH SDLSVVDGNR VRPVPMLLGH EAAGVVETPG ADLETGQRVV MTFLPRCGSC PACGTDGLTP CGPGSVANGA GTLMNGDIRL RRDGEPVFHH LGVSGFATHA VVDRRSIVPV PDDVPATVAA LLGCAVLTGG GAVLNVGRPK PGQTVVVVGL GGVGMAAALT ALAHDDVRVV GVDQLSDKLA RAAELGVHET FTPEQAADLR AEVVIEAAGH PAALETAIGL TGPGGRTITV GLPRPDARIT VSPLGFVAEG RSLIGSYLGS AVPSRDIPRF VELWRAGRLP VESLVSSTIA LDDINAGMDE LAEGRAVRQV IDFA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagatccgc ggagccgtcc tcgaacgcat cggtgcaccc cgtccctacg ccgaatcccg gccactgacc atctccgaac tcgacctcgc cgatccgggt ccggatgaac tgctggtgcg catcgagtcc gccgggctgt gccactcgga tctgtcggtc gtcgacggca accgggtgcg tccggtgccg atgctgctgg ggcacgaggc cgcgggtgtg gtcgagacgc cgggcgcgga tctcgaaacc ggtcagcgcg tggtgatgac gttcctgccg cgctgcggat cctgcccggc ctgcgggacc gacgggctga ccccctgcgg gccgggatcg gtcgccaacg gcgcgggcac gctgatgaac ggtgacatcc ggctgcgtcg cgacggcgaa cctgtgttcc accacctcgg tgtgtccggg ttcgcgacgc acgccgtggt ggaccgccgc tcgatcgtgc cggtacccga cgacgtgccc gcgacggtcg ccgcgctgtt gggttgcgcg gtgctcacgg gcggcggggc cgtgctcaac gtcggccggc cgaagccggg acagaccgtc gtggtcgtcg gtctcggcgg ggtggggatg gccgctgcgc tcaccgcgct cgcgcacgac gacgtgcggg tggtcggtgt cgaccagttg tccgacaagc tggcccgcgc agccgaactc ggcgtgcacg agaccttcac ccccgagcag gccgcggatc tgcgggccga ggtggtgatc gaggccgcgg gacatccggc cgcgctggag accgcgatcg gtttgaccgg tcctggcgga cggaccatca cggtgggcct gccgcgcccc gacgcgcgga tcacggtgtc gccgttgggt ttcgtcgccg aaggacggtc tctgatcggc agttacctcg gttcggcggt gccgtcgcgc gacatcccgc gtttcgtcga gttgtggcgc gccgggcgcc tgccggtgga gtcgctcgtg tcgtcgacca tcgcgctcga cgacatcaac gcgggcatgg acgaactggc cgagggccgt gcggtgcgtc aggtgatcga cttcgccaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa