MysmA.00277.a

Esterase/lipase/thioesterase

CENTER ID: MysmA.00277.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: native diffraction-data
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00277.a.A1.GE28595 Full length( MysmA.00277.a ) 1 315
MysmA.00277.a.AE1.GE43154 Full length( MysmA.00277.a ) 1 315
MysmA.00277.a.B1.GE43077 Full length( MysmA.00277.a ) 1 315
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00277.a.A1.PS00529 Full length( MysmA.00277.a ) 1 315
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_175727
PATRIC ID: fig|246196.19.peg.5093
RefSeq: YP_889466.1
UniProt: A0R2S8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRFELSALID PQLLSLVEES REFYADRPPG RGPAGVSELL ALRETMPEPA ECWPPPSVET VSAGGRSVPV RIHAPISRPA AGVLLEIHSG GFYLGSAADS DVRNRRLVNA LGVAVVSVDY RLAPEHPWPA APDDCETAAL WLVENAEQRF GTTRLVMTGL SAGSTLVTTT LVRLRDRGIF AFSGAVAECG TYDLSTRTPA GRLIADEYFL RAYAGHAPDR TAPDISPIFA SLEKLPPILM VVGAEDILLE DNLAMAARLV AAGVEVDLRV YPASPHAFTG HPTSMARAAL EAIDSWIRER LSAEPLTAPS VGIWS
NT Sequence
ATGAGATTTG AGCTGTCGGC ATTGATTGAT CCGCAGCTTC TTTCACTCGT CGAGGAATCC CGTGAGTTCT ACGCGGACCG ACCGCCCGGT CGCGGTCCGG CCGGCGTCAG CGAGCTCCTC GCGCTCCGTG AGACGATGCC CGAACCTGCC GAGTGTTGGC CACCACCGTC GGTGGAGACG GTGAGTGCGG GTGGGCGAAG CGTGCCGGTG CGGATCCACG CCCCGATCTC GCGGCCCGCC GCGGGCGTGC TCTTGGAGAT CCACTCCGGT GGGTTCTATC TGGGGTCGGC CGCCGACAGC GACGTCCGGA ATCGGCGACT CGTGAACGCT TTGGGCGTTG CCGTGGTGAG TGTGGATTAT CGACTCGCCC CGGAGCATCC ATGGCCTGCA GCACCAGACG ATTGCGAGAC CGCGGCGTTG TGGCTCGTGG AGAATGCCGA ACAACGATTC GGCACCACGC GCCTGGTCAT GACCGGCTTG TCCGCCGGCT CCACCCTGGT GACAACAACA TTGGTGCGAC TACGTGATCG CGGGATCTTC GCCTTCAGCG GTGCCGTTGC CGAGTGCGGG ACCTACGATC TGTCCACCCG AACTCCGGCG GGCCGCCTCA TCGCAGACGA GTATTTCCTG CGCGCGTATG CGGGCCACGC CCCGGATCGC ACGGCGCCGG ACATCTCACC GATTTTTGCG TCGCTGGAGA AGCTTCCACC GATCCTTATG GTGGTCGGCG CGGAAGACAT CCTGCTCGAG GACAACTTGG CCATGGCGGC AAGGCTTGTC GCGGCCGGCG TCGAGGTCGA TCTGCGGGTC TATCCCGCTT CGCCGCACGC ATTCACCGGA CACCCGACCT CGATGGCGCG GGCTGCTCTC GAAGCCATCG ACTCGTGGAT CCGCGAGCGA CTGAGTGCCG AGCCGTTAAC CGCGCCGAGC GTCGGCATAT GGTCG
Details for MysmA.00277.a.A1.GE28595
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 86
Validated AA Sequence
MGTLEAQTQG PGSMRFELSA LIDPQLLSLV EESREFYADR PPGRGPAGVS ELLALRETMP EPAECWPPPS VETVSAGGRS VPVRIHAPIS RPAAGVLLEI HSGGFYLGSA ADSDVRNRRL VNALGVAVVS VDYRLAPEHP WPAAPDDCET AALWLVENAE QRFGTTRLVM TGLSAGSTLV TTTLVRLRDR GIFAFSGAVA ECGTYDLSTR TPAGRLIADE YFLRAYAGHA PDRTAPDISP IFASLEKLPP ILMVVGAEXI LLEXNLAMAA XLVAAGVEVD LRVYPASPHA FX
Validated NT Sequence
atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatga gatttgagct gtcggcattg attgatccgc agcttctttc actcgtcgag gaatcccgtg agttctacgc ggaccgaccg cccggtcgcg gtccggccgg cgtcagcgag ctcctcgcgc tccgtgagac gatgcccgaa cctgccgagt gttggccacc accgtcggtg gagacggtga gtgcgggtgg gcgaagcgtg ccggtgcgga tccacgcccc gatctcgcgg cccgccgcgg gcgtgctctt ggagatccac tccggtgggt tctatctggg gtcggccgcc gacagcgacg tccggaatcg gcgactcgtg aacgctttgg gcgttgccgt ggtgagtgtg gattatcgac tcgccccgga gcatccatgg cctgcagcac cagacgattg cgagaccgcg gcgttgtggc tcgtggagaa tgccgaacaa cgattcggca ccacgcgcct ggtcatgacc ggcttgtccg ccggctccac cctggtgaca acaacattgg tgcgactacg tgatcgcggg atcttcgcct tcagcggtgc cgttgccgag tgcgggacct acgatctgtc cacccgaact ccggcgggcc gcctcatcgc agacgagtat ttcctgcgcg cgtatgcggg ccacgccccg gatcgcacgg cgccngacat ctcaccgatt tttgcgtcgc tggagaagct tccaccgatc cttatggtgg tcggcgcgga aganatcctg ctcgaggana acttggccat ggcggcnnng cttgtcgcgg ccggcgtcga ggtcgatctg cgggtctatc ccgcttcncc gcacgcattc nccnga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRFELSALI DPQLLSLVEE SREFYADRPP GRGPAGVSEL LALRETMPEP AECWPPPSVE TVSAGGRSVP VRIHAPISRP AAGVLLEIHS GGFYLGSAAD SDVRNRRLVN ALGVAVVSVD YRLAPEHPWP AAPDDCETAA LWLVENAEQR FGTTRLVMTG LSAGSTLVTT TLVRLRDRGI FAFSGAVAEC GTYDLSTRTP AGRLIADEYF LRAYAGHAPD RTAPDISPIF ASLEKLPPIL MVVGAEDILL EDNLAMAARL VAAGVEVDLR VYPASPHAFT GHPTSMARAA LEAIDSWIRE RLSAEPLTAP SVGIWS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagatttgag ctgtcggcat tgattgatcc gcagcttctt tcactcgtcg aggaatcccg tgagttctac gcggaccgac cgcccggtcg cggtccggcc ggcgtcagcg agctcctcgc gctccgtgag acgatgcccg aacctgccga gtgttggcca ccaccgtcgg tggagacggt gagtgcgggt gggcgaagcg tgccggtgcg gatccacgcc ccgatctcgc ggcccgccgc gggcgtgctc ttggagatcc actccggtgg gttctatctg gggtcggccg ccgacagcga cgtccggaat cggcgactcg tgaacgcttt gggcgttgcc gtggtgagtg tggattatcg actcgccccg gagcatccat ggcctgcagc accagacgat tgcgagaccg cggcgttgtg gctcgtggag aatgccgaac aacgattcgg caccacgcgc ctggtcatga ccggcttgtc cgccggctcc accctggtga caacaacatt ggtgcgacta cgtgatcgcg ggatcttcgc cttcagcggt gccgttgccg agtgcgggac ctacgatctg tccacccgaa ctccggcggg ccgcctcatc gcagacgagt atttcctgcg cgcgtatgcg ggccacgccc cggatcgcac ggcgccggac atctcaccga tttttgcgtc gctggagaag cttccaccga tccttatggt ggtcggcgcg gaagacatcc tgctcgagga caacttggcc atggcggcaa ggcttgtcgc ggccggcgtc gaggtcgatc tgcgggtcta tcccgcttcg ccgcacgcat tcaccggaca cccgacctcg atggcgcggg ctgctctcga agccatcgac tcgtggatcc gcgagcgact gagtgccgag ccgttaaccg cgccgagcgt cggcatatgg tcgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MysmA.00277.a.AE1.GE43154
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MRFELSALID PQLLSLVEES REFYADRPPG RGPAGVSELL ALRETMPEPA ECWPPPSVET VSAGGRSVPV RIHAPISRPA AGVLLEIHSG GFYLGSAADS DVRNRRLVNA LGVAVVSVDY RLAPEHPWPA APDDCETAAL WLVENAEQRF GTTRLVMTGL SAGSTLVTTT LVRLRDRGIF AFSGAVAECG TYDLSTRTPA GRLIADEYFL RAYAGHAPDR TAPDISPIFA SLEKLPPILM VVGAEDILLE DNLAMAARLV AAGVEVDLRV YPASPHAFTG HPTSMARAAL EAIDSWIRER LSAEPLTAPS VGIWSAGHHH HHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag ccgaccatat gccgacgctc ggcgcggtta acggctcggc actcagtcgc tcgcggatcc acgagtcgat ggcttcgaga gcagcccgcg ccatcgaggt cgggtgtccg gtgaatgcgt gcggcgaagc gggatagacc cgcagatcga cctcgacgcc ggccgcgaca agccttgccg ccatggccaa gttgtcctcg agcaggatgt cttccgcgcc gaccaccata aggatcggtg gaagcttctc cagcgacgca aaaatcggtg agatgtccgg cgccgtgcga tccggggcgt ggcccgcata cgcgcgcagg aaatactcgt ctgcgatgag gcggcccgcc ggagttcggg tggacagatc gtaggtcccg cactcggcaa cggcaccgct gaaggcgaag atcccgcgat cacgtagtcg caccaatgtt gttgtcacca gggtggagcc ggcggacaag ccggtcatga ccaggcgcgt ggtgccgaat cgttgttcgg cattctccac gagccacaac gccgcggtct cgcaatcgtc tggtgctgca ggccatggat gctccggggc gagtcgataa tccacactca ccacggcaac gcccaaagcg ttcacgagtc gccgattccg gacgtcgctg tcggcggccg accccagata gaacccaccg gagtggatct ccaagagcac gcccgcggcg ggccgcgaga tcggggcgtg gatccgcacc ggcacgcttc gcccacccgc actcaccgtc tccaccgacg gtggtggcca acactcggca ggttcgggca tcgtctcacg gagcgcgagg agctcgctga cgccggccgg accgcgaccg ggcggtcggt ccgcgtagaa ctcacgggat tcctcgacga gtgaaagaag ctgcggatca atcaatgccg acagctcaaa tctcatggga gaga
Expected Protein Sequence
MRFELSALID PQLLSLVEES REFYADRPPG RGPAGVSELL ALRETMPEPA ECWPPPSVET VSAGGRSVPV RIHAPISRPA AGVLLEIHSG GFYLGSAADS DVRNRRLVNA LGVAVVSVDY RLAPEHPWPA APDDCETAAL WLVENAEQRF GTTRLVMTGL SAGSTLVTTT LVRLRDRGIF AFSGAVAECG TYDLSTRTPA GRLIADEYFL RAYAGHAPDR TAPDISPIFA SLEKLPPILM VVGAEDILLE DNLAMAARLV AAGVEVDLRV YPASPHAFTG HPTSMARAAL EAIDSWIRER LSAEPLTAPS VGIWSGHHHH HH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gagatttgag ctgtcggcat tgattgatcc gcagcttctt tcactcgtcg aggaatcccg tgagttctac gcggaccgac cgcccggtcg cggtccggcc ggcgtcagcg agctcctcgc gctccgtgag acgatgcccg aacctgccga gtgttggcca ccaccgtcgg tggagacggt gagtgcgggt gggcgaagcg tgccggtgcg gatccacgcc ccgatctcgc ggcccgccgc gggcgtgctc ttggagatcc actccggtgg gttctatctg gggtcggccg ccgacagcga cgtccggaat cggcgactcg tgaacgcttt gggcgttgcc gtggtgagtg tggattatcg actcgccccg gagcatccat ggcctgcagc accagacgat tgcgagaccg cggcgttgtg gctcgtggag aatgccgaac aacgattcgg caccacgcgc ctggtcatga ccggcttgtc cgccggctcc accctggtga caacaacatt ggtgcgacta cgtgatcgcg ggatcttcgc cttcagcggt gccgttgccg agtgcgggac ctacgatctg tccacccgaa ctccggcggg ccgcctcatc gcagacgagt atttcctgcg cgcgtatgcg ggccacgccc cggatcgcac ggcgccggac atctcaccga tttttgcgtc gctggagaag cttccaccga tccttatggt ggtcggcgcg gaagacatcc tgctcgagga caacttggcc atggcggcaa ggcttgtcgc ggccggcgtc gaggtcgatc tgcgggtcta tcccgcttcg ccgcacgcat tcaccggaca cccgacctcg atggcgcggg ctgctctcga agccatcgac tcgtggatcc gcgagcgact gagtgccgag ccgttaaccg cgccgagcgt cggcatatgg tcggggcacc accatcatca tcattaacgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for MysmA.00277.a.B1.GE43077
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 61
PERCENT COVERAGE: 56
Validated AA Sequence
MAHHHHHHMX XELXXLIDPX LLSLVEESRE FYADRPPGRG PAGVSELLAL RETMPEPAXX WPPPSVETVS AGGRSVPVRI HAPDLAARRG RALGDPLRWV LSGVGRRQRR PESATRERFG RCRGECGLST RPGASMACST RRLRDRGVVA RGECRTTIRH HAPGHDRLVR RLHPGDNNIG ATT
Validated NT Sequence
cggttaacgg ctcggcactc agtcgctcgc ggatccacga gtcgatggct tcgagagcag cccgcgccat cgaggtcggg tgtccggtga atgcgtgcgg cgaagcggga tagacccgca gatcgacctc gacgccggcc gcgacaagcc ttgccgccat ggccaagttg tcctcgagca ggatgtcttc cgcgccgacc accataagga tcggtggaag cttctccagc gacgcaaaaa tcggtgagat gtccggcgcc gtgcgatccg gggcgtggcc cgcatacgcg cgcaggaaat actcgtctgc gatgaggcgg cccgccggag ttcgggtgga cagatcgtag gtcccgcact cggcaacggc accgctgaag gcgaagatcc cgcgatcacg tagtcgcacc aatgttgttg tcaccagggt ggagccggcg gacaagccgg tcatgaccag gcgcgtggtg ccgaatcgtt gttcggcatt ctccacgagc cacaacgccg cggtctcgca atcgtctggt gctgcaggcc atggatgctc cggggcgagt cgataatcca cactcaccac ggcaacgccc aaagcgttca cgagtcgccg attccggacg tcgctgtcgg cggccgaccc cagatagaac ccaccggagt ggatctccaa gagcacgccc gcggcgggcc gcgagatcgg gggcgtggat ccgcaccggc acgcttcgcc cacccgcact caccgtctcc accgacggtg gtggccaacn nncggcaggt tcgggcatcg tctcacggag cgcgaggagc tcgctgacgc cggccggacc gcgaccgggc ggtcggtccg cgtagaactc acgggattcc tcgacgagtg aaagaagctn nggatcaatc aatgnnnaca gctcaannnn catatggtgg tggtggtggt gagccatggt atat
Expected Protein Sequence
MAHHHHHHMR FELSALIDPQ LLSLVEESRE FYADRPPGRG PAGVSELLAL RETMPEPAEC WPPPSVETVS AGGRSVPVRI HAPISRPAAG VLLEIHSGGF YLGSAADSDV RNRRLVNALG VAVVSVDYRL APEHPWPAAP DDCETAALWL VENAEQRFGT TRLVMTGLSA GSTLVTTTLV RLRDRGIFAF SGAVAECGTY DLSTRTPAGR LIADEYFLRA YAGHAPDRTA PDISPIFASL EKLPPILMVV GAEDILLEDN LAMAARLVAA GVEVDLRVYP ASPHAFTGHP TSMARAALEA IDSWIRERLS AEPLTAPSVG IWS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagat ttgagctgtc ggcattgatt gatccgcagc ttctttcact cgtcgaggaa tcccgtgagt tctacgcgga ccgaccgccc ggtcgcggtc cggccggcgt cagcgagctc ctcgcgctcc gtgagacgat gcccgaacct gccgagtgtt ggccaccacc gtcggtggag acggtgagtg cgggtgggcg aagcgtgccg gtgcggatcc acgccccgat ctcgcggccc gccgcgggcg tgctcttgga gatccactcc ggtgggttct atctggggtc ggccgccgac agcgacgtcc ggaatcggcg actcgtgaac gctttgggcg ttgccgtggt gagtgtggat tatcgactcg ccccggagca tccatggcct gcagcaccag acgattgcga gaccgcggcg ttgtggctcg tggagaatgc cgaacaacga ttcggcacca cgcgcctggt catgaccggc ttgtccgccg gctccaccct ggtgacaaca acattggtgc gactacgtga tcgcgggatc ttcgccttca gcggtgccgt tgccgagtgc gggacctacg atctgtccac ccgaactccg gcgggccgcc tcatcgcaga cgagtatttc ctgcgcgcgt atgcgggcca cgccccggat cgcacggcgc cggacatctc accgattttt gcgtcgctgg agaagcttcc accgatcctt atggtggtcg gcgcggaaga catcctgctc gaggacaact tggccatggc ggcaaggctt gtcgcggccg gcgtcgaggt cgatctgcgg gtctatcccg cttcgccgca cgcattcacc ggacacccga cctcgatggc gcgggctgct ctcgaagcca tcgactcgtg gatccgcgag cgactgagtg ccgagccgtt aaccgcgccg agcgtcggca tatggtcgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MysmA.00277.a.A1.PS00529
PURIFICATION DATe: 2/18/2010
CONCENTRATION: 24.08mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 86
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMRFELSA LIDPQLLSLV EESREFYADR PPGRGPAGVS ELLALRETMP EPAECWPPPS VETVSAGGRS VPVRIHAPIS RPAAGVLLEI HSGGFYLGSA ADSDVRNRRL VNALGVAVVS VDYRLAPEHP WPAAPDDCET AALWLVENAE QRFGTTRLVM TGLSAGSTLV TTTLVRLRDR GIFAFSGAVA ECGTYDLSTR TPAGRLIADE YFLRAYAGHA PDRTAPDISP IFASLEKLPP ILMVVGAEXI LLEXNLAMAA XLVAAGVEVD LRVYPASPHA FX
Validated NT Sequence
atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatga gatttgagct gtcggcattg attgatccgc agcttctttc actcgtcgag gaatcccgtg agttctacgc ggaccgaccg cccggtcgcg gtccggccgg cgtcagcgag ctcctcgcgc tccgtgagac gatgcccgaa cctgccgagt gttggccacc accgtcggtg gagacggtga gtgcgggtgg gcgaagcgtg ccggtgcgga tccacgcccc gatctcgcgg cccgccgcgg gcgtgctctt ggagatccac tccggtgggt tctatctggg gtcggccgcc gacagcgacg tccggaatcg gcgactcgtg aacgctttgg gcgttgccgt ggtgagtgtg gattatcgac tcgccccgga gcatccatgg cctgcagcac cagacgattg cgagaccgcg gcgttgtggc tcgtggagaa tgccgaacaa cgattcggca ccacgcgcct ggtcatgacc ggcttgtccg ccggctccac cctggtgaca acaacattgg tgcgactacg tgatcgcggg atcttcgcct tcagcggtgc cgttgccgag tgcgggacct acgatctgtc cacccgaact ccggcgggcc gcctcatcgc agacgagtat ttcctgcgcg cgtatgcggg ccacgccccg gatcgcacgg cgccngacat ctcaccgatt tttgcgtcgc tggagaagct tccaccgatc cttatggtgg tcggcgcgga aganatcctg ctcgaggana acttggccat ggcggcnnng cttgtcgcgg ccggcgtcga ggtcgatctg cgggtctatc ccgcttcncc gcacgcattc nccnga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRFELSALI DPQLLSLVEE SREFYADRPP GRGPAGVSEL LALRETMPEP AECWPPPSVE TVSAGGRSVP VRIHAPISRP AAGVLLEIHS GGFYLGSAAD SDVRNRRLVN ALGVAVVSVD YRLAPEHPWP AAPDDCETAA LWLVENAEQR FGTTRLVMTG LSAGSTLVTT TLVRLRDRGI FAFSGAVAEC GTYDLSTRTP AGRLIADEYF LRAYAGHAPD RTAPDISPIF ASLEKLPPIL MVVGAEDILL EDNLAMAARL VAAGVEVDLR VYPASPHAFT GHPTSMARAA LEAIDSWIRE RLSAEPLTAP SVGIWS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGATTTGAG CTGTCGGCAT TGATTGATCC GCAGCTTCTT TCACTCGTCG AGGAATCCCG TGAGTTCTAC GCGGACCGAC CGCCCGGTCG CGGTCCGGCC GGCGTCAGCG AGCTCCTCGC GCTCCGTGAG ACGATGCCCG AACCTGCCGA GTGTTGGCCA CCACCGTCGG TGGAGACGGT GAGTGCGGGT GGGCGAAGCG TGCCGGTGCG GATCCACGCC CCGATCTCGC GGCCCGCCGC GGGCGTGCTC TTGGAGATCC ACTCCGGTGG GTTCTATCTG GGGTCGGCCG CCGACAGCGA CGTCCGGAAT CGGCGACTCG TGAACGCTTT GGGCGTTGCC GTGGTGAGTG TGGATTATCG ACTCGCCCCG GAGCATCCAT GGCCTGCAGC ACCAGACGAT TGCGAGACCG CGGCGTTGTG GCTCGTGGAG AATGCCGAAC AACGATTCGG CACCACGCGC CTGGTCATGA CCGGCTTGTC CGCCGGCTCC ACCCTGGTGA CAACAACATT GGTGCGACTA CGTGATCGCG GGATCTTCGC CTTCAGCGGT GCCGTTGCCG AGTGCGGGAC CTACGATCTG TCCACCCGAA CTCCGGCGGG CCGCCTCATC GCAGACGAGT ATTTCCTGCG CGCGTATGCG GGCCACGCCC CGGATCGCAC GGCGCCGGAC ATCTCACCGA TTTTTGCGTC GCTGGAGAAG CTTCCACCGA TCCTTATGGT GGTCGGCGCG GAAGACATCC TGCTCGAGGA CAACTTGGCC ATGGCGGCAA GGCTTGTCGC GGCCGGCGTC GAGGTCGATC TGCGGGTCTA TCCCGCTTCG CCGCACGCAT TCACCGGACA CCCGACCTCG ATGGCGCGGG CTGCTCTCGA AGCCATCGAC TCGTGGATCC GCGAGCGACT GAGTGCCGAG CCGTTAACCG CGCCGAGCGT CGGCATATGG TCGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA