SSGCID: Target Page

MysmA.00211.a

Pyrroline-5-carboxylate reductase (Rv0500 ortholog)

CENTER ID:	MysmA.00211.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00211.a.B1.GE37498	full length		1	286

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.933
RefSeq:	YP_006565695.1
RefSeq:	YP_885346.1
UniProt:	A0QR08

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRIAIIGGG SMGEALLSGL LRAGRQVKDM VVAEKFPERA KYLADKYSVR VTSVADAAEN AHYVVVAVKP ADVEKVIGEI AEAAANAETD TAEQVFVTIA AGVGTAYYEA KLPAGSPVIR VMPNAPVIVG GGVSALAPGR FATPEQLKEV STLFDAVGGV ITVSESQLDA VTALSGSGPA YFFLMAEAMV DAGVAVGLSR AVATDLVAQT MAGSAAMLLD RLDQVNAAGG AALDTTPAQL RATVTSPAGT TAAGLRELER GGLRAAVANA VEAAKTRSEQ LGITSE

NT Sequence

atgtcgagaa tcgcgatcat cggcggcgga agcatgggtg aggcactgtt gtccggcctg ctccgggccg gcaggcaggt caaggacatg gtcgttgcgg agaagttccc cgagcgtgcc aagtacctgg cggacaagta ctccgtgcgc gtcacgtcgg ttgccgacgc cgccgagaat gcccactacg tcgtcgtcgc cgtcaagccc gccgacgtcg agaaggtgat cggcgagatc gccgaggccg ctgccaacgc cgagaccgat acggccgagc aggttttcgt caccatcgcg gccggcgtcg gcaccgcgta ctacgaggcc aagctgcccg cgggctcgcc ggtgatccgg gtgatgccca atgcgccggt gatcgtcggc ggcggcgtca gcgcgctggc acccggtcgc ttcgcgacgc ccgagcagct caaggaggtc tcgacgctgt tcgacgcggt cggtggcgtc atcacggtct ccgagtccca actggacgcc gtcacggccc tgtccggttc gggcccggcg tacttcttcc tgatggccga ggcgatggtc gacgccgggg tggctgtcgg gctgtcgcgt gccgtggcga ccgatctggt tgcccagacg atggccggtt cggccgcgat gctgctggac cgcctggatc aggtgaacgc cgcgggaggc gcggcgctgg acaccacacc ggcccagttg cgggccaccg tcacctcgcc ggccggcaca accgccgctg gtctgcgcga actcgagcgg ggcgggctgc gcgccgcggt ggcaaatgcc gtggaagccg cgaaaacgcg ctctgagcag ctaggaatta catcggagta a

Details for MysmA.00211.a.B1.GE37498

HARVESTED ON:	10/31/2013
SEQUENCED ON:	11/5/2013
EXPECTED MW:	30kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS RIAIIGGGSM GEALLSGLLR AGRQVKDMVV AEKFPERAKY LADKYSVRVT SVADAAENAH YVVVAVKPAD VEKVIGEIAE AAANAETDTA EQVFVTIAAG VGTAYYEAKL PAGSPVIRVM PNAPVIVGGG VSALAPGRFA TPEQLKEVST LFDAVGGVIT VSESQLDAVT ALSGSGPAYF FLMAEAMVDA GVAVGLSRAV ATDLVAQTMA GSAAMLLDRL DQVNAAGGAA LDTTPAQLRA TVTSPAGTTA AGLRELERGG LRAAVANAVE AAKTRSEQLG ITSE

Validated NT Sequence

tactcactta ctccgatgta attcctagct gctcagagcg cgttttcgcg gcttccacgg catttgccac cgcggcgcgc agcccgcccc gctcgagttc gcgcagacca gcggcggttg tgccggccgg cgaggtgacg gtggcccgca actgggccgg tgtggtgtcc agcgccgcgc ctcccgcggc gttcacctga tccaggcggt ccagcagcat cgcggccgaa ccggccatcg tctgggcaac cagatcggtc gccacggcac gcgacagccc gacagccacc ccggcgtcga ccatcgcctc ggccatcagg aagaagtacg ccgggcccga accggacagg gccgtgacgg cgtccagttg ggactcggag accgtgatga cgccaccgac cgcgtcgaac agcgtcgaga cctccttgag ctgctcgggc gtcgcgaagc gaccgggtgc cagcgcgctg acgccgccgc cgacgatcac cggcgcattg ggcatcaccc ggatcaccgg cgagcccgcg ggcagcttgg cctcgtagta cgcggtgccg acgccggccg cgatggtgac gaaaacctgc tcggccgtat cggtctcggc gttggcagcg gcctcggcga tctcgccgat caccttctcg acgtcggcgg gcttgacggc gacgacgacg tagtgggcat tctcggcggc gtcggcaacc gacgtgacgc gcacggagta cttgtccgcc aggtacttgg cacgctcggg gaacttctcc gcaacgacca tgtccttgac ctgcctgccg gcccggagca ggccggacaa cagtgcctca cccatgcttc cgccgccgat gatcgcgatt ctcgacatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcga gaatcgcgat catcggcggc ggaagcatgg gtgaggcact gttgtccggc ctgctccggg ccggcaggca ggtcaaggac atggtcgttg cggagaagtt ccccgagcgt gccaagtacc tggcggacaa gtactccgtg cgcgtcacgt cggttgccga cgccgccgag aatgcccact acgtcgtcgt cgccgtcaag cccgccgacg tcgagaaggt gatcggcgag atcgccgagg ccgctgccaa cgccgagacc gatacggccg agcaggtttt cgtcaccatc gcggccggcg tcggcaccgc gtactacgag gccaagctgc ccgcgggctc gccggtgatc cgggtgatgc ccaatgcgcc ggtgatcgtc ggcggcggcg tcagcgcgct ggcacccggt cgcttcgcga cgcccgagca gctcaaggag gtctcgacgc tgttcgacgc ggtcggtggc gtcatcacgg tctccgagtc ccaactggac gccgtcacgg ccctgtccgg ttcgggcccg gcgtacttct tcctgatggc cgaggcgatg gtcgacgccg gggtggctgt cgggctgtcg cgtgccgtgg cgaccgatct ggttgcccag acgatggccg gttcggccgc gatgctgctg gaccgcctgg atcaggtgaa cgccgcggga ggcgcggcgc tggacaccac accggcccag ttgcgggcca ccgtcacctc gccggccggc acaaccgccg ctggtctgcg cgaactcgag cggggcgggc tgcgcgccgc ggtggcaaat gccgtggaag ccgcgaaaac gcgctctgag cagctaggaa ttacatcgga gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat