MysmA.00113.a

Polyketide synthase

CENTER ID: MysmA.00113.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00113.a.A1.GE28569 Full length( MysmA.00113.a ) 1 433
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00113.a.A1.PS00574 Full length( MysmA.00113.a ) 1 433

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.4416
RefSeq: YP_888785.1
UniProt: A0R0U7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSGEDPVVI VGMALEAPGG VTTADDYWTL LTEEREALGR FPRDRGWDIG ALFDGSRRDG FKPINDLGGF LSGAGEFDPA FFGISPREAV AMDPQQRVAL RVAWRALENS GINPDDLAGH DAGCYIGASG LEYGPPLSEF SHHSGHLITG TSLGVIAGRI AYTLDLAGPA VTVDTSCSSA LTALHTAVGA LRAGDCDLAV TGAVCVMGSP GYFVEFSKQH ALSDDGRCRP YSAHASGTVW AEGAAMFVLQ RRSAAVRDGR RILAEIRATA LNQDGRTTGL TAPSGAAQER LFRKAIDRAG IRPEDVGMIE GHGTGTRLGD RTELRSLAQT YGATAPGAGA VLGSVKSNVG HAQAAAGGLG LAKVLLAGQR AAIPATLHVD QPSGEIDWDA QGLRLATKLT PWPAVDGERI AAVSAFGMSG TNAHAIVAIP EAA
NT Sequence
ATGAGCTCTG GTGAGGACCC GGTCGTCATC GTCGGGATGG CGCTCGAGGC CCCCGGCGGG GTCACGACCG CCGACGACTA CTGGACGCTG CTCACCGAGG AACGCGAGGC ACTCGGGCGG TTCCCGCGCG ACCGTGGCTG GGACATCGGG GCACTGTTCG ACGGCTCCCG CCGCGACGGC TTCAAGCCCA TCAACGATCT GGGCGGCTTC CTGTCCGGTG CGGGTGAATT CGATCCGGCG TTCTTCGGCA TCTCGCCGCG CGAGGCCGTC GCCATGGATC CGCAGCAACG CGTCGCGTTG CGCGTCGCGT GGCGGGCGCT GGAGAACAGC GGGATCAACC CCGACGATCT CGCCGGCCAC GACGCCGGCT GCTACATCGG CGCCTCCGGC CTGGAGTACG GCCCGCCGCT TTCCGAGTTC TCCCACCACA GCGGGCATCT CATCACCGGG ACCTCGCTCG GGGTCATCGC CGGCCGCATC GCATACACGC TGGACCTGGC CGGGCCCGCC GTCACGGTCG ACACCTCGTG CTCGTCGGCG CTCACCGCGT TGCACACCGC GGTCGGCGCG CTGCGCGCGG GAGACTGCGA TCTGGCGGTG ACCGGCGCGG TGTGCGTGAT GGGCTCACCG GGGTACTTCG TCGAATTCTC CAAACAGCAC GCACTGTCCG ACGACGGCCG CTGCCGCCCC TACAGCGCCC ACGCGAGCGG CACGGTGTGG GCCGAGGGCG CGGCGATGTT CGTCCTGCAA CGCCGGTCCG CCGCAGTGCG CGACGGCCGC CGCATCCTGG CCGAGATCCG TGCCACCGCA CTCAACCAGG ACGGACGCAC CACCGGGCTC ACCGCGCCCA GCGGTGCGGC GCAGGAACGC CTGTTCCGCA AGGCCATCGA CCGGGCCGGG ATCCGCCCCG AGGACGTCGG GATGATCGAG GGCCACGGCA CCGGAACCCG ACTGGGCGAC CGCACCGAAC TGCGCTCACT CGCACAGACC TACGGCGCGA CGGCGCCCGG TGCGGGTGCC GTGCTGGGTT CGGTGAAATC GAACGTCGGC CACGCACAGG CCGCCGCGGG CGGACTCGGG CTGGCCAAGG TGCTGCTCGC GGGGCAGCGT GCGGCGATCC CGGCGACCCT GCACGTCGAC CAGCCCAGTG GCGAGATCGA CTGGGATGCG CAGGGACTGC GTCTGGCCAC CAAGCTCACG CCGTGGCCCG CGGTCGACGG CGAGCGCATC GCGGCGGTGT CGGCGTTCGG GATGAGCGGC ACCAACGCGC ACGCCATCGT CGCGATCCCG GAGGCCGCG
Details for MysmA.00113.a.A1.GE28569
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 47kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 54
PERCENT COVERAGE: 95
Validated AA Sequence
MSSGEDPVVI VGMALEAPGG VTTADDYXTL LTEEREALGR FPRDRGWDIG ALFDGSRRDG FKPINDLGGF LSGAGEFDPA FFGISPREAV AMDPQQRVAL RVAWRALENS GINPDDLAGH DAGCYIGASG LEYGPPLSEF SXXXGHLITG TSLGVIAGRI AYTLDLXRAR RHGRHLVLVG AHRVAHRGRR AARGRLRSGG DRRGVRDGLT GVLRRILQTA RTVRRRPLPP LQRPRERHGV GRGRGDVRPA TPVRRSARRP PHPGRDPCHR TQPGRTHHRA HRAQRCGAGT PVPQGHRPGR DPPRGRRDDR GPRHRNPTGR PHRTALTRTD LRRDGARCGC RAGFGEIERR PRTGRRGRTR AGQGAARGAA CGDPGDPARR PAQWRDRLGC AGTASGHQAH AVARGRRRAH RGGVGVRDER HQRARHRRDP GGRVI
Validated NT Sequence
ttattacgcg gcctccggga tcgcgacgat ggcgtgcgcg ttggtgccgc tcatcccgaa cgccgacacc gccgcgatgc gctcgccgtc gaccgcgggc cacggcgtga gcttggtggc cagacgcagt ccctgcgcat cccagtcgat ctcgccactg ggctggtcga cgtgcagggt cgccgggatc gccgcacgct gccccgcgag cagcaccttg gccagcccga gtccgcccgc ggcggcctgt gcgtggccga cgttcgattt caccgaaccc agcacggcac ccgcaccggg cgccgtcgcg ccgtaggtct gtgcgagtga gcgcagttcg gtgcggtcgc ccagtcgggt tccggtgccg tggccctcga tcatcccgac gtcctcgggg cggatcccgg cccggtcgat ggccttgcgg aacaggcgtt cctgcgccgc accgctgggc gcggtgagcc cggtggtgcg tccgtcctgg ttgagtgcgg tggcacggat ctcggccagg atgcggcggc cgtcgcgcac tgcggcggac cggcgttgca ggacgaacat cgccgcgccc tcggcccaca ccgtgccgct cgcgtgggcg ctgtaggggc ggcagcggcc gtcgtcggac agtgcgtgct gtttggagaa ttcgacgaag taccccggtg agcccatcac gcacaccgcg ccggtcaccg ccagatcgca gtctcccgcg cgcagcgcgc cgaccgcggt gtgcaacgcg gtgagcgccg acgagcacga ggtgtcgacc gtgacggcgg gcccggncca ggtccagcgt gtatgcgatg cggccggcga tgaccccgag cgaggtcccg gtgatgagat gcccgntgnn nngggagaac tcggaaagcg gcgggccgta ctccaggccg gaggcgccga tgtagcagcc ggcgtcgtgg ccggcgagat cgtcggggtt gatcccgctg ttctccagcg cccgccacgc gacgcgcaac gcgacgcgtt gctgcggatc catggcgacg gcctcgcgcg gcgagatgcc gaagaacgcc ggatcgaatt cacccgcacc ggacaggaag ccgcccagat cgttgatggg cttgaagccg tcgcggcggg agccgtcgaa cagtgccccg atgtcccagc cacggtcgcg cgggaaccgc ccgagtgcct cgcgttcctc ggtgagcagc gtccngtagt cgtcggcggt cgtgaccccg ccgggggcct cgagcgccat cccgacgatg acgaccgggt cctcaccaga gctcatcgaa c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSGEDPVV IVGMALEAPG GVTTADDYWT LLTEEREALG RFPRDRGWDI GALFDGSRRD GFKPINDLGG FLSGAGEFDP AFFGISPREA VAMDPQQRVA LRVAWRALEN SGINPDDLAG HDAGCYIGAS GLEYGPPLSE FSHHSGHLIT GTSLGVIAGR IAYTLDLAGP AVTVDTSCSS ALTALHTAVG ALRAGDCDLA VTGAVCVMGS PGYFVEFSKQ HALSDDGRCR PYSAHASGTV WAEGAAMFVL QRRSAAVRDG RRILAEIRAT ALNQDGRTTG LTAPSGAAQE RLFRKAIDRA GIRPEDVGMI EGHGTGTRLG DRTELRSLAQ TYGATAPGAG AVLGSVKSNV GHAQAAAGGL GLAKVLLAGQ RAAIPATLHV DQPSGEIDWD AQGLRLATKL TPWPAVDGER IAAVSAFGMS GTNAHAIVAI PEAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagctctggt gaggacccgg tcgtcatcgt cgggatggcg ctcgaggccc ccggcggggt cacgaccgcc gacgactact ggacgctgct caccgaggaa cgcgaggcac tcgggcggtt cccgcgcgac cgtggctggg acatcggggc actgttcgac ggctcccgcc gcgacggctt caagcccatc aacgatctgg gcggcttcct gtccggtgcg ggtgaattcg atccggcgtt cttcggcatc tcgccgcgcg aggccgtcgc catggatccg cagcaacgcg tcgcgttgcg cgtcgcgtgg cgggcgctgg agaacagcgg gatcaacccc gacgatctcg ccggccacga cgccggctgc tacatcggcg cctccggcct ggagtacggc ccgccgcttt ccgagttctc ccaccacagc gggcatctca tcaccgggac ctcgctcggg gtcatcgccg gccgcatcgc atacacgctg gacctggccg ggcccgccgt cacggtcgac acctcgtgct cgtcggcgct caccgcgttg cacaccgcgg tcggcgcgct gcgcgcggga gactgcgatc tggcggtgac cggcgcggtg tgcgtgatgg gctcaccggg gtacttcgtc gaattctcca aacagcacgc actgtccgac gacggccgct gccgccccta cagcgcccac gcgagcggca cggtgtgggc cgagggcgcg gcgatgttcg tcctgcaacg ccggtccgcc gcagtgcgcg acggccgccg catcctggcc gagatccgtg ccaccgcact caaccaggac ggacgcacca ccgggctcac cgcgcccagc ggtgcggcgc aggaacgcct gttccgcaag gccatcgacc gggccgggat ccgccccgag gacgtcggga tgatcgaggg ccacggcacc ggaacccgac tgggcgaccg caccgaactg cgctcactcg cacagaccta cggcgcgacg gcgcccggtg cgggtgccgt gctgggttcg gtgaaatcga acgtcggcca cgcacaggcc gccgcgggcg gactcgggct ggccaaggtg ctgctcgcgg ggcagcgtgc ggcgatcccg gcgaccctgc acgtcgacca gcccagtggc gagatcgact gggatgcgca gggactgcgt ctggccacca agctcacgcc gtggcccgcg gtcgacggcg agcgcatcgc ggcggtgtcg gcgttcggga tgagcggcac caacgcgcac gccatcgtcg cgatcccgga ggccgcgaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MysmA.00113.a.A1.PS00574
PURIFICATION DATe: 4/15/2010
CONCENTRATION: 9.89mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 100µl
PERCENT IDENTITY: 54
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MSSGEDPVVI VGMALEAPGG VTTADDYXTL LTEEREALGR FPRDRGWDIG ALFDGSRRDG FKPINDLGGF LSGAGEFDPA FFGISPREAV AMDPQQRVAL RVAWRALENS GINPDDLAGH DAGCYIGASG LEYGPPLSEF SXXXGHLITG TSLGVIAGRI AYTLDLXRAR RHGRHLVLVG AHRVAHRGRR AARGRLRSGG DRRGVRDGLT GVLRRILQTA RTVRRRPLPP LQRPRERHGV GRGRGDVRPA TPVRRSARRP PHPGRDPCHR TQPGRTHHRA HRAQRCGAGT PVPQGHRPGR DPPRGRRDDR GPRHRNPTGR PHRTALTRTD LRRDGARCGC RAGFGEIERR PRTGRRGRTR AGQGAARGAA CGDPGDPARR PAQWRDRLGC AGTASGHQAH AVARGRRRAH RGGVGVRDER HQRARHRRDP GGRVI
Validated NT Sequence
ttattacgcg gcctccggga tcgcgacgat ggcgtgcgcg ttggtgccgc tcatcccgaa cgccgacacc gccgcgatgc gctcgccgtc gaccgcgggc cacggcgtga gcttggtggc cagacgcagt ccctgcgcat cccagtcgat ctcgccactg ggctggtcga cgtgcagggt cgccgggatc gccgcacgct gccccgcgag cagcaccttg gccagcccga gtccgcccgc ggcggcctgt gcgtggccga cgttcgattt caccgaaccc agcacggcac ccgcaccggg cgccgtcgcg ccgtaggtct gtgcgagtga gcgcagttcg gtgcggtcgc ccagtcgggt tccggtgccg tggccctcga tcatcccgac gtcctcgggg cggatcccgg cccggtcgat ggccttgcgg aacaggcgtt cctgcgccgc accgctgggc gcggtgagcc cggtggtgcg tccgtcctgg ttgagtgcgg tggcacggat ctcggccagg atgcggcggc cgtcgcgcac tgcggcggac cggcgttgca ggacgaacat cgccgcgccc tcggcccaca ccgtgccgct cgcgtgggcg ctgtaggggc ggcagcggcc gtcgtcggac agtgcgtgct gtttggagaa ttcgacgaag taccccggtg agcccatcac gcacaccgcg ccggtcaccg ccagatcgca gtctcccgcg cgcagcgcgc cgaccgcggt gtgcaacgcg gtgagcgccg acgagcacga ggtgtcgacc gtgacggcgg gcccggncca ggtccagcgt gtatgcgatg cggccggcga tgaccccgag cgaggtcccg gtgatgagat gcccgntgnn nngggagaac tcggaaagcg gcgggccgta ctccaggccg gaggcgccga tgtagcagcc ggcgtcgtgg ccggcgagat cgtcggggtt gatcccgctg ttctccagcg cccgccacgc gacgcgcaac gcgacgcgtt gctgcggatc catggcgacg gcctcgcgcg gcgagatgcc gaagaacgcc ggatcgaatt cacccgcacc ggacaggaag ccgcccagat cgttgatggg cttgaagccg tcgcggcggg agccgtcgaa cagtgccccg atgtcccagc cacggtcgcg cgggaaccgc ccgagtgcct cgcgttcctc ggtgagcagc gtccngtagt cgtcggcggt cgtgaccccg ccgggggcct cgagcgccat cccgacgatg acgaccgggt cctcaccaga gctcatcgaa c
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSGEDPVV IVGMALEAPG GVTTADDYWT LLTEEREALG RFPRDRGWDI GALFDGSRRD GFKPINDLGG FLSGAGEFDP AFFGISPREA VAMDPQQRVA LRVAWRALEN SGINPDDLAG HDAGCYIGAS GLEYGPPLSE FSHHSGHLIT GTSLGVIAGR IAYTLDLAGP AVTVDTSCSS ALTALHTAVG ALRAGDCDLA VTGAVCVMGS PGYFVEFSKQ HALSDDGRCR PYSAHASGTV WAEGAAMFVL QRRSAAVRDG RRILAEIRAT ALNQDGRTTG LTAPSGAAQE RLFRKAIDRA GIRPEDVGMI EGHGTGTRLG DRTELRSLAQ TYGATAPGAG AVLGSVKSNV GHAQAAAGGL GLAKVLLAGQ RAAIPATLHV DQPSGEIDWD AQGLRLATKL TPWPAVDGER IAAVSAFGMS GTNAHAIVAI PEAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCTCTGGT GAGGACCCGG TCGTCATCGT CGGGATGGCG CTCGAGGCCC CCGGCGGGGT CACGACCGCC GACGACTACT GGACGCTGCT CACCGAGGAA CGCGAGGCAC TCGGGCGGTT CCCGCGCGAC CGTGGCTGGG ACATCGGGGC ACTGTTCGAC GGCTCCCGCC GCGACGGCTT CAAGCCCATC AACGATCTGG GCGGCTTCCT GTCCGGTGCG GGTGAATTCG ATCCGGCGTT CTTCGGCATC TCGCCGCGCG AGGCCGTCGC CATGGATCCG CAGCAACGCG TCGCGTTGCG CGTCGCGTGG CGGGCGCTGG AGAACAGCGG GATCAACCCC GACGATCTCG CCGGCCACGA CGCCGGCTGC TACATCGGCG CCTCCGGCCT GGAGTACGGC CCGCCGCTTT CCGAGTTCTC CCACCACAGC GGGCATCTCA TCACCGGGAC CTCGCTCGGG GTCATCGCCG GCCGCATCGC ATACACGCTG GACCTGGCCG GGCCCGCCGT CACGGTCGAC ACCTCGTGCT CGTCGGCGCT CACCGCGTTG CACACCGCGG TCGGCGCGCT GCGCGCGGGA GACTGCGATC TGGCGGTGAC CGGCGCGGTG TGCGTGATGG GCTCACCGGG GTACTTCGTC GAATTCTCCA AACAGCACGC ACTGTCCGAC GACGGCCGCT GCCGCCCCTA CAGCGCCCAC GCGAGCGGCA CGGTGTGGGC CGAGGGCGCG GCGATGTTCG TCCTGCAACG CCGGTCCGCC GCAGTGCGCG ACGGCCGCCG CATCCTGGCC GAGATCCGTG CCACCGCACT CAACCAGGAC GGACGCACCA CCGGGCTCAC CGCGCCCAGC GGTGCGGCGC AGGAACGCCT GTTCCGCAAG GCCATCGACC GGGCCGGGAT CCGCCCCGAG GACGTCGGGA TGATCGAGGG CCACGGCACC GGAACCCGAC TGGGCGACCG CACCGAACTG CGCTCACTCG CACAGACCTA CGGCGCGACG GCGCCCGGTG CGGGTGCCGT GCTGGGTTCG GTGAAATCGA ACGTCGGCCA CGCACAGGCC GCCGCGGGCG GACTCGGGCT GGCCAAGGTG CTGCTCGCGG GGCAGCGTGC GGCGATCCCG GCGACCCTGC ACGTCGACCA GCCCAGTGGC GAGATCGACT GGGATGCGCA GGGACTGCGT CTGGCCACCA AGCTCACGCC GTGGCCCGCG GTCGACGGCG AGCGCATCGC GGCGGTGTCG GCGTTCGGGA TGAGCGGCAC CAACGCGCAC GCCATCGTCG CGATCCCGGA GGCCGCGAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA