MysmA.00058.a
Thioredoxin reductase
| CENTER ID: | MysmA.00058.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00058.a.B1.GE40209 | Full length(MysmA.00058.a) | 1 | 311 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00058.a.B1.PS38133 | Full length(MysmA.00058.a) | 1 | 311 | ||
| MysmA.00058.a.EM11.PS38495 | Full length(MysmA.00058.a) | 1 | 311 |
Structures
5UTH
DEPOSITED: 2/15/2017
DETERMINATION: XRay
CLONE: MysmA.00058.a.B1.GE40209
PROTEIN: MysmA.00058.a.B1.PS38133
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|246196.19.peg.6747 |
| UniProt: | A0R7I9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTSQTVHDV IIIGSGPAGY TAAIYAARAQ LKPLVFEGTQ FGGALMTTTE VENYPGFREG ITGPELMDQM REQALRFGAD LRMEDVDAVQ LEGPVKTVVV GDETHQARAV ILAMGAAARH LGVPGEEALT GMGVSTCATC DGFFFRDQDI VVVGGGDSAM EEATFLTRFA RSVTLIHRRD EFRASKIMLE RARANEKITF LTNTEITQIE GDPKVTGVRL RDTVTGEESK LDVTGVFVAI GHDPRSELVR GQVELDDEGY VKVQGRTTYT SLDGVFAAGD LVDHTYRQAI TAAGSGCAAS IDAERWLAEQ D
NT Sequence
ATGTCCACTT CACAGACGGT TCACGACGTC ATCATCATCG GTTCCGGCCC GGCCGGGTAC ACCGCGGCGA TCTATGCGGC CCGCGCTCAG CTCAAGCCCC TGGTGTTCGA GGGCACACAG TTCGGCGGCG CGTTGATGAC CACCACCGAG GTGGAGAACT ACCCGGGCTT CCGTGAGGGC ATCACGGGTC CGGAGCTGAT GGATCAGATG CGCGAGCAGG CGCTGCGGTT CGGTGCGGAC CTGCGCATGG AAGACGTCGA CGCAGTACAG CTCGAAGGCC CCGTCAAGAC CGTCGTGGTC GGCGACGAGA CCCACCAGGC CCGCGCGGTC ATCCTGGCGA TGGGCGCGGC CGCACGCCAC CTCGGCGTGC CCGGCGAGGA AGCGTTGACC GGCATGGGTG TGAGCACCTG CGCGACGTGT GACGGCTTCT TCTTCCGCGA CCAGGACATC GTGGTGGTCG GCGGCGGCGA CTCGGCGATG GAGGAAGCGA CGTTCCTCAC ACGCTTTGCC CGCAGTGTGA CGTTGATCCA CCGCCGCGAC GAGTTCCGCG CGTCCAAGAT CATGCTGGAG CGCGCCCGCG CCAACGAGAA GATCACGTTC CTCACCAACA CCGAGATCAC CCAGATCGAG GGTGACCCGA AGGTCACCGG TGTGCGCCTG CGTGACACCG TGACGGGCGA GGAGTCCAAG CTCGACGTCA CCGGCGTGTT CGTCGCGATC GGCCACGACC CGCGATCGGA GCTCGTGCGC GGTCAGGTCG AACTCGACGA CGAGGGCTAC GTCAAGGTCC AGGGCCGCAC CACCTACACG TCGCTCGACG GGGTGTTCGC GGCAGGCGAT CTGGTGGACC ACACTTACCG CCAGGCCATC ACGGCCGCGG GCAGCGGCTG TGCGGCATCC ATCGACGCCG AGCGCTGGCT CGCCGAACAA GAC
Details for MysmA.00058.a.B1.GE40209
| HARVESTED ON: | 5/9/2016 |
| SEQUENCED ON: | 5/23/2016 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMS TSQTVHDVII IGSGPAGYTA AIYAARAQLK PLVFEGTQFG GALMTTTEVE NYPGFREGIT GPELMDQMRE QALRFGADLR MEDVDAVQLE GPVKTVVVGD ETHQARAVIL AMGAAARHLG VPGEEALTGM GVSTCATCDG FFFRDQDIVV VGGGDSAMEE ATFLTRFARS VTLIHRRDEF RASKIMLERA RANEKITFLT NTEITQIEGD PKVTGVRLRD TVTGEESKLD VTGVFVAIGH DPRSELVRGQ VELDDEGYVK VQGRTTYTSL DGVFAAGDLV DHTYRQAITA AGSGCAASID XXXLAR
Validated NT Sequence
ggcgagccag nnnnnnnnng tcgatggatg ccgcacagcc gctgcccgcg gccgtgatgg cctggcggta agtgtggtcc accagatcgc ctgccgcgaa caccccgtcg agcgacgtgt aggtggtgcg gccctggacc ttgacgtagc cctcgtcgtc gagttcgacc tgaccgcgca cgagctccga tcgcgggtcg tggccgatcg cgacgaacac gccggtgacg tcgagcttgg actcctcgcc cgtcacggtg tcacgcaggc gcacaccggt gaccttcggg tcaccctcga tctgggtgat ctcggtgttg gtgaggaacg tgatcttctc gttggcgcgg gcgcgctcca gcatgatctt ggacgcgcgg aactcgtcgc ggcggtggat caacgtcaca ctgcgggcaa agcgtgtgag gaacgtcgct tcctccatcg ccgagtcgcc gccgccgacc accacgatgt cctggtcgcg gaagaagaag ccgtcacacg tcgcgcaggt gctcacaccc atgccggtca acgcttcctc gccgggcacg ccgaggtggc gtgcggccgc gcccatcgcc aggatgaccg cgcgggcctg gtgggtctcg tcgccgacca cgacggtctt gacggggcct tcgagctgta ctgcgtcgac gtcttccatg cgcaggtccg caccgaaccg cagcgcctgc tcgcgcatct gatccatcag ctccggaccc gtgatgccct cacggaagcc cgggtagttc tccacctcgg tggtggtcat caacgcgccg ccgaactgtg tgccctcgaa caccaggggc ttgagctgag cgcgggccgc atagatcgcc gcggtgtacc cggccgggcc ggaaccgatg atgatgacgt cgtgaaccgt ctgtgaagtg gacatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMS TSQTVHDVII IGSGPAGYTA AIYAARAQLK PLVFEGTQFG GALMTTTEVE NYPGFREGIT GPELMDQMRE QALRFGADLR MEDVDAVQLE GPVKTVVVGD ETHQARAVIL AMGAAARHLG VPGEEALTGM GVSTCATCDG FFFRDQDIVV VGGGDSAMEE ATFLTRFARS VTLIHRRDEF RASKIMLERA RANEKITFLT NTEITQIEGD PKVTGVRLRD TVTGEESKLD VTGVFVAIGH DPRSELVRGQ VELDDEGYVK VQGRTTYTSL DGVFAAGDLV DHTYRQAITA AGSGCAASID AERWLAEQD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca cttcacagac ggttcacgac gtcatcatca tcggttccgg cccggccggg tacaccgcgg cgatctatgc ggcccgcgct cagctcaagc ccctggtgtt cgagggcaca cagttcggcg gcgcgttgat gaccaccacc gaggtggaga actacccggg cttccgtgag ggcatcacgg gtccggagct gatggatcag atgcgcgagc aggcgctgcg gttcggtgcg gacctgcgca tggaagacgt cgacgcagta cagctcgaag gccccgtcaa gaccgtcgtg gtcggcgacg agacccacca ggcccgcgcg gtcatcctgg cgatgggcgc ggccgcacgc cacctcggcg tgcccggcga ggaagcgttg accggcatgg gtgtgagcac ctgcgcgacg tgtgacggct tcttcttccg cgaccaggac atcgtggtgg tcggcggcgg cgactcggcg atggaggaag cgacgttcct cacacgcttt gcccgcagtg tgacgttgat ccaccgccgc gacgagttcc gcgcgtccaa gatcatgctg gagcgcgccc gcgccaacga gaagatcacg ttcctcacca acaccgagat cacccagatc gagggtgacc cgaaggtcac cggtgtgcgc ctgcgtgaca ccgtgacggg cgaggagtcc aagctcgacg tcaccggcgt gttcgtcgcg atcggccacg acccgcgatc ggagctcgtg cgcggtcagg tcgaactcga cgacgagggc tacgtcaagg tccagggccg caccacctac acgtcgctcg acggggtgtt cgcggcaggc gatctggtgg accacactta ccgccaggcc atcacggccg cgggcagcgg ctgtgcggca tccatcgacg ccgagcgctg gctcgccgaa caagactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for MysmA.00058.a.B1.PS38133
| PURIFICATION DATe: | 11/29/2016 |
| CONCENTRATION: | 69.12mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS TSQTVHDVII IGSGPAGYTA AIYAARAQLK PLVFEGTQFG GALMTTTEVE NYPGFREGIT GPELMDQMRE QALRFGADLR MEDVDAVQLE GPVKTVVVGD ETHQARAVIL AMGAAARHLG VPGEEALTGM GVSTCATCDG FFFRDQDIVV VGGGDSAMEE ATFLTRFARS VTLIHRRDEF RASKIMLERA RANEKITFLT NTEITQIEGD PKVTGVRLRD TVTGEESKLD VTGVFVAIGH DPRSELVRGQ VELDDEGYVK VQGRTTYTSL DGVFAAGDLV DHTYRQAITA AGSGCAASID XXXLAR
Validated NT Sequence
ggcgagccag nnnnnnnnng tcgatggatg ccgcacagcc gctgcccgcg gccgtgatgg cctggcggta agtgtggtcc accagatcgc ctgccgcgaa caccccgtcg agcgacgtgt aggtggtgcg gccctggacc ttgacgtagc cctcgtcgtc gagttcgacc tgaccgcgca cgagctccga tcgcgggtcg tggccgatcg cgacgaacac gccggtgacg tcgagcttgg actcctcgcc cgtcacggtg tcacgcaggc gcacaccggt gaccttcggg tcaccctcga tctgggtgat ctcggtgttg gtgaggaacg tgatcttctc gttggcgcgg gcgcgctcca gcatgatctt ggacgcgcgg aactcgtcgc ggcggtggat caacgtcaca ctgcgggcaa agcgtgtgag gaacgtcgct tcctccatcg ccgagtcgcc gccgccgacc accacgatgt cctggtcgcg gaagaagaag ccgtcacacg tcgcgcaggt gctcacaccc atgccggtca acgcttcctc gccgggcacg ccgaggtggc gtgcggccgc gcccatcgcc aggatgaccg cgcgggcctg gtgggtctcg tcgccgacca cgacggtctt gacggggcct tcgagctgta ctgcgtcgac gtcttccatg cgcaggtccg caccgaaccg cagcgcctgc tcgcgcatct gatccatcag ctccggaccc gtgatgccct cacggaagcc cgggtagttc tccacctcgg tggtggtcat caacgcgccg ccgaactgtg tgccctcgaa caccaggggc ttgagctgag cgcgggccgc atagatcgcc gcggtgtacc cggccgggcc ggaaccgatg atgatgacgt cgtgaaccgt ctgtgaagtg gacatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMS TSQTVHDVII IGSGPAGYTA AIYAARAQLK PLVFEGTQFG GALMTTTEVE NYPGFREGIT GPELMDQMRE QALRFGADLR MEDVDAVQLE GPVKTVVVGD ETHQARAVIL AMGAAARHLG VPGEEALTGM GVSTCATCDG FFFRDQDIVV VGGGDSAMEE ATFLTRFARS VTLIHRRDEF RASKIMLERA RANEKITFLT NTEITQIEGD PKVTGVRLRD TVTGEESKLD VTGVFVAIGH DPRSELVRGQ VELDDEGYVK VQGRTTYTSL DGVFAAGDLV DHTYRQAITA AGSGCAASID AERWLAEQD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCA CTTCACAGAC GGTTCACGAC GTCATCATCA TCGGTTCCGG CCCGGCCGGG TACACCGCGG CGATCTATGC GGCCCGCGCT CAGCTCAAGC CCCTGGTGTT CGAGGGCACA CAGTTCGGCG GCGCGTTGAT GACCACCACC GAGGTGGAGA ACTACCCGGG CTTCCGTGAG GGCATCACGG GTCCGGAGCT GATGGATCAG ATGCGCGAGC AGGCGCTGCG GTTCGGTGCG GACCTGCGCA TGGAAGACGT CGACGCAGTA CAGCTCGAAG GCCCCGTCAA GACCGTCGTG GTCGGCGACG AGACCCACCA GGCCCGCGCG GTCATCCTGG CGATGGGCGC GGCCGCACGC CACCTCGGCG TGCCCGGCGA GGAAGCGTTG ACCGGCATGG GTGTGAGCAC CTGCGCGACG TGTGACGGCT TCTTCTTCCG CGACCAGGAC ATCGTGGTGG TCGGCGGCGG CGACTCGGCG ATGGAGGAAG CGACGTTCCT CACACGCTTT GCCCGCAGTG TGACGTTGAT CCACCGCCGC GACGAGTTCC GCGCGTCCAA GATCATGCTG GAGCGCGCCC GCGCCAACGA GAAGATCACG TTCCTCACCA ACACCGAGAT CACCCAGATC GAGGGTGACC CGAAGGTCAC CGGTGTGCGC CTGCGTGACA CCGTGACGGG CGAGGAGTCC AAGCTCGACG TCACCGGCGT GTTCGTCGCG ATCGGCCACG ACCCGCGATC GGAGCTCGTG CGCGGTCAGG TCGAACTCGA CGACGAGGGC TACGTCAAGG TCCAGGGCCG CACCACCTAC ACGTCGCTCG ACGGGGTGTT CGCGGCAGGC GATCTGGTGG ACCACACTTA CCGCCAGGCC ATCACGGCCG CGGGCAGCGG CTGTGCGGCA TCCATCGACG CCGAGCGCTG GCTCGCCGAA CAAGACTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T
Details for MysmA.00058.a.EM11.PS38495
| PURIFICATION DATe: | 8/28/2018 |
| CONCENTRATION: | 39.93mg/ml |
| OBSERVED MW: | 34kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL21 DE3 |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS TSQTVHDVII IGSGPAGYTA AIYAARAQLK PLVFEGTSFG GALMTTTDVE NYPGFREGIT GPELMDQMRE
QALRFGADLR MEDVDAVQLE GPVKTVVVGD ETHQARAVIL AMGAAARHLG VPGEEALLGM GVSSCATCDG FFFRDQDIVV
VGGGDSAMEE ATFLTRFARS VTLIHRRDEF RASKIMLERA RANEKITFLT NTEITQIEGD PKVTGVRLRD TVTGEESKLD
VTGVFVAIGH DPRSELVRGQ VELDDEGYVK VQGRTTYTSL DGVFAAGDLV DHTYRQAVTA AGSGCAASID AERWLAEQD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA
TGGCTCACCA CCACCACCAC CATATGTCCA CTTCACAGAC GGTTCACGAC GTCATCATCA TCGGTTCCGG CCCGGCCGGG
TACACCGCGG CGATCTATGC GGCCCGCGCT CAGCTCAAGC CCCTGGTGTT CGAGGGCACA TCCTTCGGCG GCGCGTTGAT
GACCACCACC GACGTGGAGA ACTACCCGGG CTTCCGTGAG GGCATCACGG GTCCGGAGCT GATGGATCAG ATGCGCGAGC
AGGCGCTGCG GTTCGGTGCG GACCTGCGCA TGGAAGACGT CGACGCAGTA CAGCTCGAAG GCCCCGTCAA GACCGTCGTG
GTCGGCGACG AGACCCACCA GGCCCGCGCG GTCATCCTGG CGATGGGCGC GGCCGCACGC CACCTCGGCG TGCCCGGCGA
GGAAGCGTTG CTCGGCATGG GTGTGAGCTC CTGCGCGACG TGTGACGGCT TCTTCTTCCG CGACCAGGAC ATCGTGGTGG
TCGGCGGCGG CGACTCGGCG ATGGAGGAAG CGACGTTCCT CACACGCTTT GCCCGCAGTG TGACGTTGAT CCACCGCCGC
GACGAGTTCC GCGCGTCCAA GATCATGCTG GAGCGCGCCC GCGCCAACGA GAAGATCACG TTCCTCACCA ACACCGAGAT
CACCCAGATC GAGGGTGACC CGAAGGTCAC CGGTGTGCGC CTGCGTGACA CCGTGACGGG CGAGGAGTCC AAGCTCGACG
TCACCGGCGT GTTCGTCGCG ATCGGCCACG ACCCGCGATC GGAGCTCGTG CGCGGTCAGG TCGAACTCGA CGACGAGGGC
TACGTCAAGG TCCAGGGCCG CACCACCTAC ACGTCGCTCG ACGGGGTGTT CGCGGCAGGC GATCTGGTGG ACCACACTTA
CCGCCAGGCC GTAACGGCCG CGGGCAGCGG CTGTGCGGCA TCCATCGACG CCGAGCGCTG GCTCGCCGAA CAAGACTAAG
TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC
ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG
GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC
GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA
TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG
ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA
ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA
TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC
TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT
GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT
GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT
CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG
GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG
TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA
TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC
ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA
GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT
GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG
GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG
GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC
AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT
TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA
TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT
GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG
TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG
GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG
CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT
GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG
AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT
GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC
CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC
GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT
TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA
GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG
GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT
CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA
TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT
TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA
TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG
CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA
GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA
AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT
GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG
TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG
GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC
CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG
GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT
CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG
CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC
GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG
ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC
GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA
AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG
CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG
GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG
AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT
GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT