MypaA.19484.a
Dephospho-CoA kinase CoaE
| CENTER ID: | MypaA.19484.a |
| ORGANISM: | Mycobacterium paratuberculosis ATCC BAA-968 / K-10 |
| ASSOCIATED DISEASE: | Johne's disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.19484.a.B1.GE40757 | full length | 1 | 407 | ||
| MypaA.19484.a.B2.GE40758 | nterm domain | 1 | 204 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.19484.a.B1.PW38003 | full length | 1 | 407 |
Structures
6N39
DEPOSITED: 11/14/2018
DETERMINATION: XRay
CLONE: MypaA.19484.a.B1.GE40757
PROTEIN: MypaA.19484.a.B1.PW38003
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|262316.17.peg.1397 |
| UniProt: | Q740M4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRIGLTGGI GAGKSALSSA FAQCGAVIVD GDVIAREVVR PGTEGLAALV EAFGRDILLA DGSLDRPALA AKAFADDAAR QTLNGIVHPL VGARRAEIIA SVPADSVVVE DIPLLVESGM APLFPLVVIV YADVEVRLRR LVEQRGMAEA DARARIAAQA SDEQRRAVAD IWLDNSGSPA ELVQRAQQVW NERIVPFAHN LSTRQIARAP VRLVPPDPEW PAQAQRIVNR LKTASGHRAL RVDHVGSTAL PGDPDFAAKD VIDIQITVES LAAADELVEP LLAAGYPRLE HITADVAKPD ARSTVERYDH TGDPALWHKR IHASADPGRP TNVHIRVDGW PGQQFALLFV DWLTADPDAR ADYLAVKRSA EQRADGDIDA YVAVKEPWFR DAYRRAWDWA DSTGWKP
NT Sequence
atgctgcgca tcgggttgac cggcggcatc ggcgccggca agtcggcgct gtcctccgcg ttcgcgcagt gcggtgcggt catcgtcgac ggtgacgtca tcgcccgtga ggtggtccgg cccggtaccg agggcctggc ggcgctggtc gaggcgttcg gacgcgacat cctgctcgcg gacggatcgc tggaccggcc ggcgttggcg gccaaggctt tcgccgacga cgccgcccgt cagacgctca acgggatcgt gcatccgctg gtgggtgcgc gccgcgccga gataatcgcg tcggtgcccg cggattccgt tgtggtcgag gacattccgc tgctggtgga atccgggatg gcgccgctgt tcccgctcgt cgtcatcgtg tacgccgatg tcgaggtgcg gctgcggcgg ctggtcgagc agcgcggcat ggccgaagcc gacgcccgcg cccggatcgc cgctcaggcc agcgacgagc agcgccgcgc cgtcgccgac atctggctgg acaactcggg cagcccggcg gagttggtgc aacgggccca gcaggtgtgg aacgagcgca tcgtgccgtt cgcccacaac ctgagcacgc gtcagatcgc ccgggcgccg gtgcggttgg tgccgcccga tcccgagtgg ccggcccagg cgcagcgaat cgtcaaccgg ctcaagaccg catccggcca tcgggcgctg cgcgtcgacc acgtcggctc cacggcgttg cccggggatc cggatttcgc ggccaaggac gtcatcgaca tccagatcac cgtggaatcc ctggccgccg ccgacgaact cgtcgaaccg ttgctggccg ccggataccc tcgcctcgaa cacatcaccg ccgacgtcgc caagcccgac gcccgcagca ccgtcgagcg ctacgatcac accggcgatc cggccttgtg gcacaagcgg attcacgcct cggcggaccc gggccggccc accaacgtgc acatccgggt ggacggctgg cccggccagc agttcgccct gctgttcgtg gactggttga ccgccgaccc cgatgctcgg gccgactatc tggccgtcaa gcgcagcgcc gaacagcggg ccgacggtga catcgacgcc tacgtcgccg tcaaggagcc gtggtttcgc gacgcctacc ggcgggcctg ggattgggcc gactcgacgg gctggaaacc ctag
Details for MypaA.19484.a.B1.GE40757
| HARVESTED ON: | 11/21/2016 |
| SEQUENCED ON: | 11/22/2016 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHML RIGLTGGIGA GKSALSSAFA QCGAVIVDGD VIAREVVRPG TEGLAALVEA FGRDILLADG SLDRPALAAK AFADDAARQT LNGIVHPLVG ARRAEIIASV PADSVVVEDI PLLVESGMAP LFPLVVIVYA DVEVRLRRLV EQRGMAEADA RARIAAQASD EQRRAVADIW LDNSGSPAEL VQRAQQVWNE RIVPFAHNLS TRQIARAPVR LVPPDPEWPA QAQRIVNRLK TASGHRALRV DHVGSTALPG DPDFAAKDVI DIQITVESLA AADELVEPLL AAGYPRLEHI TADVAKPDAR STVERYDHTG DPALWHKRIH ASADPGRPTN VHIRVDGWPG QQFALLFVDW LTADPDARAD YLAVKRSAEQ RADGDIDAYV AVKEPWFRDA YRRAWDW
Validated NT Sequence
gcccaatccc aggcccgccg gtaggcgtcg cgaaaccacg gctccttgac ggcgacgtag gcgtcgatgt caccgtcggc ccgctgttcg gcgctgcgct tgacggccag atagtcggcc cgagcatcgg ggtcggcggt caaccagtcc acgaacagca gggcgaactg ctggccgggc cagccgtcca cccggatgtg cacgttggtg ggccggcccg ggtccgccga ggcgtgaatc cgcttgtgcc acaaggccgg atcgccggtg tgatcgtagc gctcgacggt gctgcgggcg tcgggcttgg cgacgtcggc ggtgatgtgt tcgaggcgag ggtatccggc ggccagcaac ggttcgacga gttcgtcggc ggcggccagg gattccacgg tgatctggat gtcgatgacg tccttggccg cgaaatccgg atccccgggc aacgccgtgg agccgacgtg gtcgacgcgc agcgcccgat ggccggatgc ggtcttgagc cggttgacga ttcgctgcgc ctgggccggc cactcgggat cgggcggcac caaccgcacc ggcgcccggg cgatctgacg cgtgctcagg ttgtgggcga acggcacgat gcgctcgttc cacacctgct gggcccgttg caccaactcc gccgggctgc ccgagttgtc cagccagatg tcggcgacgg cgcggcgctg ctcgtcgctg gcctgagcgg cgatccgggc gcgggcgtcg gcttcggcca tgccgcgctg ctcgaccagc cgccgcagcc gcacctcgac atcggcgtac acgatgacga cgagcgggaa cagcggcgcc atcccggatt ccaccagcag cggaatgtcc tcgaccacaa cggaatccgc gggcaccgac gcgattatct cggcgcggcg cgcacccacc agcggatgca cgatcccgtt gagcgtctga cgggcggcgt cgtcggcgaa agccttggcc gccaacgccg gccggtccag cgatccgtcc gcgagcagga tgtcgcgtcc gaacgcctcg accagcgccg ccaggccctc ggtaccgggc cggaccacct cacgggcgat gacgtcaccg tcgacgatga ccgcaccgca ctgcgcgaac gcggaggaca gcgccgactt gccggcgccg atgccgccgg tcaacccgat gcgcagcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHML RIGLTGGIGA GKSALSSAFA QCGAVIVDGD VIAREVVRPG TEGLAALVEA FGRDILLADG SLDRPALAAK AFADDAARQT LNGIVHPLVG ARRAEIIASV PADSVVVEDI PLLVESGMAP LFPLVVIVYA DVEVRLRRLV EQRGMAEADA RARIAAQASD EQRRAVADIW LDNSGSPAEL VQRAQQVWNE RIVPFAHNLS TRQIARAPVR LVPPDPEWPA QAQRIVNRLK TASGHRALRV DHVGSTALPG DPDFAAKDVI DIQITVESLA AADELVEPLL AAGYPRLEHI TADVAKPDAR STVERYDHTG DPALWHKRIH ASADPGRPTN VHIRVDGWPG QQFALLFVDW LTADPDARAD YLAVKRSAEQ RADGDIDAYV AVKEPWFRDA YRRAWDWADS TGWKP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgc gcatcgggtt gaccggcggc atcggcgccg gcaagtcggc gctgtcctcc gcgttcgcgc agtgcggtgc ggtcatcgtc gacggtgacg tcatcgcccg tgaggtggtc cggcccggta ccgagggcct ggcggcgctg gtcgaggcgt tcggacgcga catcctgctc gcggacggat cgctggaccg gccggcgttg gcggccaagg ctttcgccga cgacgccgcc cgtcagacgc tcaacgggat cgtgcatccg ctggtgggtg cgcgccgcgc cgagataatc gcgtcggtgc ccgcggattc cgttgtggtc gaggacattc cgctgctggt ggaatccggg atggcgccgc tgttcccgct cgtcgtcatc gtgtacgccg atgtcgaggt gcggctgcgg cggctggtcg agcagcgcgg catggccgaa gccgacgccc gcgcccggat cgccgctcag gccagcgacg agcagcgccg cgccgtcgcc gacatctggc tggacaactc gggcagcccg gcggagttgg tgcaacgggc ccagcaggtg tggaacgagc gcatcgtgcc gttcgcccac aacctgagca cgcgtcagat cgcccgggcg ccggtgcggt tggtgccgcc cgatcccgag tggccggccc aggcgcagcg aatcgtcaac cggctcaaga ccgcatccgg ccatcgggcg ctgcgcgtcg accacgtcgg ctccacggcg ttgcccgggg atccggattt cgcggccaag gacgtcatcg acatccagat caccgtggaa tccctggccg ccgccgacga actcgtcgaa ccgttgctgg ccgccggata ccctcgcctc gaacacatca ccgccgacgt cgccaagccc gacgcccgca gcaccgtcga gcgctacgat cacaccggcg atccggcctt gtggcacaag cggattcacg cctcggcgga cccgggccgg cccaccaacg tgcacatccg ggtggacggc tggcccggcc agcagttcgc cctgctgttc gtggactggt tgaccgccga ccccgatgct cgggccgact atctggccgt caagcgcagc gccgaacagc gggccgacgg tgacatcgac gcctacgtcg ccgtcaagga gccgtggttt cgcgacgcct accggcgggc ctgggattgg gccgactcga cgggctggaa accctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for MypaA.19484.a.B2.GE40758
| HARVESTED ON: | 1/31/2017 |
| SEQUENCED ON: | 11/22/2016 |
| EXPECTED MW: | 23kDa |
| OBSERVED MW: | 23kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHML RIGLTGGIGA GKSALSSAFA QCGAVIVDGD VIAREVVRPG TEGLAALVEA FGRDILLADG SLDRPALAAK AFADDAARQT LNGIVHPLVG ARRAEIIASV PADSVVVEDI PLLVESGMAP LFPLVVIVYA DVEVRLRRLV EQRGMAEADA RARIAAQASD EQRRAVADIW LDNSGSPAEL VQRAQQVWNE RIVPFAHNLS TR
Validated NT Sequence
atcttactca cttaacgcgt gctcaggttg tgggcgaacg gcacgatgcg ctcgttccac acctgctggg cccgttgcac caactccgcc gggctgcccg agttgtccag ccagatgtcg gcgacggcgc ggcgctgctc gtcgctggcc tgagcggcga tccgggcgcg ggcgtcggct tcggccatgc cgcgctgctc gaccagccgc cgcagccgca cctcgacatc ggcgtacacg atgacgacga gcgggaacag cggcgccatc ccggattcca ccagcagcgg aatgtcctcg accacaacgg aatccgcggg caccgacgcg attatctcgg cgcggcgcgc acccaccagc ggatgcacga tcccgttgag cgtctgacgg gcggcgtcgt cggcgaaagc cttggccgcc aacgccggcc ggtccagcga tccgtccgcg agcaggatgt cgcgtccgaa cgcctcgacc agcgccgcca ggccctcggt accgggccgg accacctcac gggcgatgac gtcaccgtcg acgatgaccg caccgcactg cgcgaacgcg gaggacagcg ccgacttgcc ggcgccgatg ccgccggtca acccgatgcg cagcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHML RIGLTGGIGA GKSALSSAFA QCGAVIVDGD VIAREVVRPG TEGLAALVEA FGRDILLADG SLDRPALAAK AFADDAARQT LNGIVHPLVG ARRAEIIASV PADSVVVEDI PLLVESGMAP LFPLVVIVYA DVEVRLRRLV EQRGMAEADA RARIAAQASD EQRRAVADIW LDNSGSPAEL VQRAQQVWNE RIVPFAHNLS TR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgc gcatcgggtt gaccggcggc atcggcgccg gcaagtcggc gctgtcctcc gcgttcgcgc agtgcggtgc ggtcatcgtc gacggtgacg tcatcgcccg tgaggtggtc cggcccggta ccgagggcct ggcggcgctg gtcgaggcgt tcggacgcga catcctgctc gcggacggat cgctggaccg gccggcgttg gcggccaagg ctttcgccga cgacgccgcc cgtcagacgc tcaacgggat cgtgcatccg ctggtgggtg cgcgccgcgc cgagataatc gcgtcggtgc ccgcggattc cgttgtggtc gaggacattc cgctgctggt ggaatccggg atggcgccgc tgttcccgct cgtcgtcatc gtgtacgccg atgtcgaggt gcggctgcgg cggctggtcg agcagcgcgg catggccgaa gccgacgccc gcgcccggat cgccgctcag gccagcgacg agcagcgccg cgccgtcgcc gacatctggc tggacaactc gggcagcccg gcggagttgg tgcaacgggc ccagcaggtg tggaacgagc gcatcgtgcc gttcgcccac aacctgagca cgcgttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for MypaA.19484.a.B1.PW38003
| PURIFICATION DATe: | 1/3/2017 |
| CONCENTRATION: | 19.5mg/ml |
| OBSERVED MW: | 45kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML RIGLTGGIGA GKSALSSAFA QCGAVIVDGD VIAREVVRPG TEGLAALVEA FGRDILLADG SLDRPALAAK AFADDAARQT LNGIVHPLVG ARRAEIIASV PADSVVVEDI PLLVESGMAP LFPLVVIVYA DVEVRLRRLV EQRGMAEADA RARIAAQASD EQRRAVADIW LDNSGSPAEL VQRAQQVWNE RIVPFAHNLS TRQIARAPVR LVPPDPEWPA QAQRIVNRLK TASGHRALRV DHVGSTALPG DPDFAAKDVI DIQITVESLA AADELVEPLL AAGYPRLEHI TADVAKPDAR STVERYDHTG DPALWHKRIH ASADPGRPTN VHIRVDGWPG QQFALLFVDW LTADPDARAD YLAVKRSAEQ RADGDIDAYV AVKEPWFRDA YRRAWDW
Validated NT Sequence
gcccaatccc aggcccgccg gtaggcgtcg cgaaaccacg gctccttgac ggcgacgtag gcgtcgatgt caccgtcggc ccgctgttcg gcgctgcgct tgacggccag atagtcggcc cgagcatcgg ggtcggcggt caaccagtcc acgaacagca gggcgaactg ctggccgggc cagccgtcca cccggatgtg cacgttggtg ggccggcccg ggtccgccga ggcgtgaatc cgcttgtgcc acaaggccgg atcgccggtg tgatcgtagc gctcgacggt gctgcgggcg tcgggcttgg cgacgtcggc ggtgatgtgt tcgaggcgag ggtatccggc ggccagcaac ggttcgacga gttcgtcggc ggcggccagg gattccacgg tgatctggat gtcgatgacg tccttggccg cgaaatccgg atccccgggc aacgccgtgg agccgacgtg gtcgacgcgc agcgcccgat ggccggatgc ggtcttgagc cggttgacga ttcgctgcgc ctgggccggc cactcgggat cgggcggcac caaccgcacc ggcgcccggg cgatctgacg cgtgctcagg ttgtgggcga acggcacgat gcgctcgttc cacacctgct gggcccgttg caccaactcc gccgggctgc ccgagttgtc cagccagatg tcggcgacgg cgcggcgctg ctcgtcgctg gcctgagcgg cgatccgggc gcgggcgtcg gcttcggcca tgccgcgctg ctcgaccagc cgccgcagcc gcacctcgac atcggcgtac acgatgacga cgagcgggaa cagcggcgcc atcccggatt ccaccagcag cggaatgtcc tcgaccacaa cggaatccgc gggcaccgac gcgattatct cggcgcggcg cgcacccacc agcggatgca cgatcccgtt gagcgtctga cgggcggcgt cgtcggcgaa agccttggcc gccaacgccg gccggtccag cgatccgtcc gcgagcagga tgtcgcgtcc gaacgcctcg accagcgccg ccaggccctc ggtaccgggc cggaccacct cacgggcgat gacgtcaccg tcgacgatga ccgcaccgca ctgcgcgaac gcggaggaca gcgccgactt gccggcgccg atgccgccgg tcaacccgat gcgcagcata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHML RIGLTGGIGA GKSALSSAFA QCGAVIVDGD VIAREVVRPG TEGLAALVEA FGRDILLADG SLDRPALAAK AFADDAARQT LNGIVHPLVG ARRAEIIASV PADSVVVEDI PLLVESGMAP LFPLVVIVYA DVEVRLRRLV EQRGMAEADA RARIAAQASD EQRRAVADIW LDNSGSPAEL VQRAQQVWNE RIVPFAHNLS TRQIARAPVR LVPPDPEWPA QAQRIVNRLK TASGHRALRV DHVGSTALPG DPDFAAKDVI DIQITVESLA AADELVEPLL AAGYPRLEHI TADVAKPDAR STVERYDHTG DPALWHKRIH ASADPGRPTN VHIRVDGWPG QQFALLFVDW LTADPDARAD YLAVKRSAEQ RADGDIDAYV AVKEPWFRDA YRRAWDWADS TGWKP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTGC GCATCGGGTT GACCGGCGGC ATCGGCGCCG GCAAGTCGGC GCTGTCCTCC GCGTTCGCGC AGTGCGGTGC GGTCATCGTC GACGGTGACG TCATCGCCCG TGAGGTGGTC CGGCCCGGTA CCGAGGGCCT GGCGGCGCTG GTCGAGGCGT TCGGACGCGA CATCCTGCTC GCGGACGGAT CGCTGGACCG GCCGGCGTTG GCGGCCAAGG CTTTCGCCGA CGACGCCGCC CGTCAGACGC TCAACGGGAT CGTGCATCCG CTGGTGGGTG CGCGCCGCGC CGAGATAATC GCGTCGGTGC CCGCGGATTC CGTTGTGGTC GAGGACATTC CGCTGCTGGT GGAATCCGGG ATGGCGCCGC TGTTCCCGCT CGTCGTCATC GTGTACGCCG ATGTCGAGGT GCGGCTGCGG CGGCTGGTCG AGCAGCGCGG CATGGCCGAA GCCGACGCCC GCGCCCGGAT CGCCGCTCAG GCCAGCGACG AGCAGCGCCG CGCCGTCGCC GACATCTGGC TGGACAACTC GGGCAGCCCG GCGGAGTTGG TGCAACGGGC CCAGCAGGTG TGGAACGAGC GCATCGTGCC GTTCGCCCAC AACCTGAGCA CGCGTCAGAT CGCCCGGGCG CCGGTGCGGT TGGTGCCGCC CGATCCCGAG TGGCCGGCCC AGGCGCAGCG AATCGTCAAC CGGCTCAAGA CCGCATCCGG CCATCGGGCG CTGCGCGTCG ACCACGTCGG CTCCACGGCG TTGCCCGGGG ATCCGGATTT CGCGGCCAAG GACGTCATCG ACATCCAGAT CACCGTGGAA TCCCTGGCCG CCGCCGACGA ACTCGTCGAA CCGTTGCTGG CCGCCGGATA CCCTCGCCTC GAACACATCA CCGCCGACGT CGCCAAGCCC GACGCCCGCA GCACCGTCGA GCGCTACGAT CACACCGGCG ATCCGGCCTT GTGGCACAAG CGGATTCACG CCTCGGCGGA CCCGGGCCGG CCCACCAACG TGCACATCCG GGTGGACGGC TGGCCCGGCC AGCAGTTCGC CCTGCTGTTC GTGGACTGGT TGACCGCCGA CCCCGATGCT CGGGCCGACT ATCTGGCCGT CAAGCGCAGC GCCGAACAGC GGGCCGACGG TGACATCGAC GCCTACGTCG CCGTCAAGGA GCCGTGGTTT CGCGACGCCT ACCGGCGGGC CTGGGATTGG GCCGACTCGA CGGGCTGGAA ACCCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT