SSGCID: Target Page

MypaA.19371.a

Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX [Includes: Lysine--tRNA ligase (EC 6.1.1.6) (Lysyl-tRNA synthetase) (LysRS); Phosphatidylglycerol lysyltransferase (EC 2.3.2.3) (Lysylphosphatidylglycerol synthetase) (LPG synthetase)]

CENTER ID:	MypaA.19371.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.19371.a.B2.GE40756	soluble domain		304	1177

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.1425
UniProt:	Q740J9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRAGRSRQF SSVEEAFSTS AARPGPRGRR SGRENTAKFV PATLSASTSF SRIEGISSRS VTLASPGSRS GSGPRSGPRL GPRNRSTSRY RWVPAAAGWT VGVIATVSLL GSVSPLIRYL IKVPREFIND YLFNFPDTSI AWSFVLALLA AALTARKRIA WLLLLGNMIL AAALNVADIA AGDNTAAEIF GENLGFAVHI VAIVLLVLAY REFWAKVRKG ALVKAAAVLV AGDVVGILVS WGLVELFPGT LARQDRLPYV VNRVVGFALA DPDLFTGRPH VFLNAIFGLF GALALIMATI VLFQSQRAEN ALTGEDESAI RGLLELYGKN DSLGYFATRR DKSVVFAQSG RAAITYRVEI GVCLASGDPI GDPRAWPQAV DAWLGLCQTY GWAPGVMGAS TQGARTYREA GLNALELGDE AILRTSEFKL SGPDMRGVRQ AVTRARRAGL TVRIRRHRDI SPEAMADTIA RADAWRDTQT ERGFSMALGR LGDPADGDCL LVEAIDRDGS VVAMLSLVPW GTTGVSLDLM RRSPSSPNGT IELMVSELAL NAESLGITRI SLNFAMFRSA FEQGAQLGAG PVARLWRGLL LFFSRWWQLE TLYRSNMKYQ PDWVPRYACY EDARLIPRVG VASVIAEGFL VLPFSRRDKV HTGHHPAVPA RLAQSGLLHH DGSAPDVSGL RPERTDAEEA RSRLPEQVRV RLAKLKVLQR NGVDAYPVGC PPSHTVAQAL DADDQQDITV AGRILRIRDF GGVLFAQLRD WSGEMQVLLD NSRLERGRTA DFTAAIDLGD LVEVSGQMGF SKKGTRSLIV TDWRMIGKCL RPLPNKWKGL TDPEARVRTR YVDLAVNPES RELIAARSEV LRSVRQTLFA KGFIEVETPI LQQIHGGATA RPFVTHINTY DMDLFLRIAP ELYLKRLCVG GVERVFELGR AFRNEGVDFS HNPEFTLLEA YQAHADYRVW IDGCRELIQN AAQAAHGEQT VLRPGADGRL QPVDISGIWA VKTVHDAVSE ALGEQVDPGT SLSTLRKLSD AARIPYRAHW DAGAVVLELY EHLVEDRTEE PTFYVDFPTS VSPLTRPHRS RPGVAERWDL VAWGVELATA YSELTDPVEQ RRRLQEQSLL AAGGDPEAME LDEDFLQAME YAMPPTGGLG MGVDRLVMLI TGRSIRETLP FPLAKPH

NT Sequence

ttgcgccggg ctggtcgcag ccgtcagttt agcagtgttg aggaggcttt tagcacttcg gccgcccgac cgggcccgcg cgggcgtcga tccggccggg aaaacaccgc gaaattcgtg ccggccactc ttagcgcttc gacaagcttt tcacggatag agggcatatc ctcgcggtct gtgacactcg catctcctgg ttcgaggtct ggttcgggtc ctcgttcggg gccgcggttg gggcctcgca accggtcgac ctcgcggtat cgctgggtgc cggcggccgc cgggtggacc gtgggtgtga tcgccacggt gtcgctgctg ggcagcgtgt ccccgctgat ccggtacctg atcaaggtcc cgcgcgagtt catcaacgac tacctgttca acttccccga cacaagcatc gcctggtcgt tcgtgctggc cctgctggcg gcggcgctga cggcacgcaa gcggatcgcc tggctgctgt tgctgggcaa catgatcctg gccgccgccc tcaacgtcgc cgacatcgcc gccggtgaca acaccgccgc cgagatcttc ggcgagaacc tgggtttcgc cgtccacatc gtcgcgatcg tcctgctggt gctggcgtat cgggagttct gggccaaggt ccgcaagggc gcgctggtca aggcggccgc ggtgctggtc gccggggacg tggtgggcat cctggtgtcc tggggcctgg tcgagttgtt ccccggcacc ctggcccgcc aggaccggct gccctacgtg gtcaaccggg tggtcggctt cgcgctagcc gacccggacc ttttcaccgg ccgcccgcac gtgttcctca acgcgatctt cgggttgttc ggcgcgctcg cgctgatcat ggcgaccatc gtgctctttc aatcccagcg cgcggagaac gcgctgaccg gtgaggacga gtcggccatc cgcggactgc tggagttgta cggcaagaac gactcgctgg gctacttcgc cacccgccgc gacaaatcgg tggtcttcgc ccagagcgga cgcgccgcca tcacctaccg ggtcgagatc ggggtctgtt tggccagcgg cgacccgatc ggcgatcccc gcgcgtggcc gcaggcggtc gacgcctggc tgggcctgtg ccagacctac ggctgggcgc ccggcgtgat gggcgccagc acgcaggggg ctcggaccta tcgcgaggcc ggcctcaacg cgctcgagct gggtgacgag gccatcctgc gcacctccga gttcaagctg tccggcccgg acatgcgcgg cgtccgccag gcggtgaccc gggcccgccg ggccgggttg acggtgcgga tccgccggca ccgcgacatc tcccccgagg cgatggccga caccatcgcc cgcgccgacg cctggcgtga cacccagacc gagcgcggct tctccatggc gctgggccgg ctcggtgacc cggccgacgg ggactgcctg ctggtggagg ccatcgaccg ggacggcagc gtggtcgcga tgctgtcgct ggtcccgtgg ggcaccaccg gtgtgtcgct ggacttgatg cgccggtcgc cgagctcacc caacggcacc atcgagctga tggtcagcga gctggcgttg aacgccgaaa gccttggtat cacccgcatt tcgctgaact tcgcgatgtt tcggtcggcg ttcgaacagg gcgcccagct gggcgccggc ccggtcgcgc ggctgtggcg ggggttgctg ctgttcttct cccggtggtg gcagctggag accctgtacc gctcgaacat gaagtaccaa cccgattggg ttccgcgcta cgcctgctac gaggatgccc ggctgatccc ccgggtcggc gtcgcgtcgg tgatcgccga gggcttcctg gtgttgccgt tcagccggcg cgacaaggtg cacaccggcc atcaccccgc cgtgccggcc cggctggcgc agtcgggcct gctgcaccac gacggctcgg caccggacgt gagcgggctg cgcccggagc gcaccgacgc cgaggaggcg cgctcgcggc tgcccgagca ggtccgggtg cgcctggcca agctgaaggt gttgcagcgc aacggtgttg acgcctatcc ggtgggctgt ccgcccagcc acaccgtcgc gcaggcgctg gacgccgacg accagcagga catcaccgtc gccgggcgga tcctgcggat ccgcgacttc ggtggtgtgc tgttcgccca gctgcgggac tggtcggggg aaatgcaagt gctgctggac aattcgcgtc tggagcgggg ccgcacggcc gacttcaccg cggccatcga tctcggcgac ctggtcgagg tgtccggtca gatggggttc tccaagaaag gcacccggtc gctgatcgtc accgactggc ggatgatcgg taagtgcttg cggccattgc cgaataagtg gaaggggctc accgatccgg aggcccgggt gcgaacccgc tacgtcgacc tggcggtcaa ccccgagtcg cgggagttga tcgcggcgcg cagcgaggtg ctgcgctcgg tccggcagac cctgttcgcc aagggattca tcgaggtcga gaccccgatc ctgcagcaga tccacggcgg cgccaccgcc cggccgttcg tcacgcacat caacacctac gacatggacc tgttcctgcg catcgcgccg gagctgtatc tgaagcggct gtgcgtcggc ggcgtggagc gcgtcttcga gttgggccgg gcgttccgca acgagggggt cgacttcagc cacaacccgg agttcaccct gctggaggcc taccaggcgc acgccgacta tcgggtgtgg atcgacgggt gccgcgagct gatccagaac gccgcccagg ccgcgcacgg cgagcagacc gtgctgcggc ccggcgccga cggccggctg caaccggtcg acatctccgg gatctgggcg gtcaagaccg tgcacgacgc ggtgtccgag gcactcggcg agcaggtcga ccccggcacg tcgctgtcca ccctgcgcaa actgtccgac gcggcgcgca tcccctaccg cgcgcactgg gacgccggcg cggtggtgct cgagctctac gagcacctgg tcgaggaccg gaccgaggag ccgacgttct acgtcgactt ccccacctcg gtgtcgccgc tgacccggcc gcaccgcagc cggcccgggg tcgcggagcg gtgggacctg gtggcctggg gtgtcgagct ggccaccgcc tacagcgagc tgaccgatcc ggtggagcag cgccgccgcc tgcaggagca gtcgctgtta gctgccggcg gggatcccga ggcgatggaa ctcgacgagg acttcctgca ggcgatggag tacgcgatgc cgccgacggg cgggctgggc atgggcgtcg atcgcctcgt catgctcata acgggccgca gcatccgcga aaccttgccg tttccgctgg ccaagccgca ctag

Details for MypaA.19371.a.B2.GE40756

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	98kDa
OBSERVED MW:	98kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHQS QRAENALTGE DESAIRGLLE LYGKNDSLGY FATRRDKSVV FAQSGRAAIT YRVEIGVCLA SGDPIGDPRA WPQAVDAWLG LCQTYGWAPG VMGASTQGAR TYREAGLNAL ELGDEAILRT SEFKLSGPDM RGVRQAVTRA RRAGLTVRIR RHRDISPEAM ADTIARADAW RDTQTERGFS MALGRLGDPA DGDCLLVEAI DRDGSVVAML SLVPWGTTGV SLDLMRRSPS SPNGTIELMV SELALNAESL GITRISLNFA MFRSAFEQGA QLGAGPVARL WRGLLLFFSR WWQLETLYRS NMKYQPDWVP RYACYEDARL IPRVGVASVI AEGFLVLPFS RRDKVHTGHH PAVPARLAQS GLLHHDGSAP DVSGLRPERT DAEEARSRLP EQVRVRLAKL KVLQRNGVDA YPVGCPPSHT VAQALDADDQ QDITVAGRIL RIRDFGGVLF AQLRDWSGEM QVLLDNSRLE RGRTADFTAA IDLGDLVEVS GQMGFSKKGT RSLIVTDWRM IGKCLRPLPN KWKGLTDPEA RVRTRYVDLA VNPESRELIA ARSEVLRSVR QTLFAKGFIE VETPILQQIH GGATARPFVT HINTYDMDLF LRIAPELYLK RLCVGGVERV FELGRAFRNE GVDFSHNPEF TLLEAYQAHA DYRVWIDGCR ELIQNAAQAA HGEQTVLRPG ADGRLQPVDI SGIWAVKTVH DAVSEALGEQ VDPGTSLSTL RKLSDAARIP YRAHWDAGAV VLELYEHLVE DRTEEPTFYV DFPTSVSPLT RPHRSRPGVA ERWDLVAWGV ELATAYSELT DPVEQRRRLQ EQSLLAAGGD PEAMELDEDF LQAMEYAMPP TGGLGMGVDR LVMLITGRSI RETLPFPLAK PH

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaatccc agcgcgcgga gaacgcgctg accggtgagg acgagtcggc catccgcgga ctgctggagt tgtacggcaa gaacgactcg ctgggctact tcgccacccg ccgcgacaaa tcggtggtct tcgcccagag cggacgcgcc gccatcacct accgggtcga gatcggggtc tgtttggcca gcggcgaccc gatcggcgat ccccgcgcgt ggccgcaggc ggtcgacgcc tggctgggcc tgtgccagac ctacggctgg gcgcccggcg tgatgggcgc cagcacgcag ggggctcgga cctatcgcga ggccggcctc aacgcgctcg agctgggtga cgaggccatc ctgcgcacct ccgagttcaa gctgtccggc ccggacatgc gcggcgtccg ccaggcggtg acccgggccc gccgggccgg gttgacggtg cggatccgcc ggcaccgcga catctccccc gaggcgatgg ccgacaccat cgcccgcgcc gacgcctggc gtgacaccca gaccgagcgc ggcttctcca tggcgctggg ccggctcggt gacccggccg acggggactg cctgctggtg gaggccatcg accgggacgg cagcgtggtc gcgatgctgt cgctggtccc gtggggcacc accggtgtgt cgctggactt gatgcgccgg tcgccgagct cacccaacgg caccatcgag ctgatggtca gcgagctggc gttgaacgcc gaaagccttg gtatcacccg catttcgctg aacttcgcga tgtttcggtc ggcgttcgaa cagggcgccc agctgggcgc cggcccggtc gcgcggctgt ggcgggggtt gctgctgttc ttctcccggt ggtggcagct ggagaccctg taccgctcga acatgaagta ccaacccgat tgggttccgc gctacgcctg ctacgaggat gcccggctga tcccccgggt cggcgtcgcg tcggtgatcg ccgagggctt cctggtgttg ccgttcagcc ggcgcgacaa ggtgcacacc ggccatcacc ccgccgtgcc ggcccggctg gcgcagtcgg gcctgctgca ccacgacggc tcggcaccgg acgtgagcgg gctgcgcccg gagcgcaccg acgccgagga ggcgcgctcg cggctgcccg agcaggtccg ggtgcgcctg gccaagctga aggtgttgca gcgcaacggt gttgacgcct atccggtggg ctgtccgccc agccacaccg tcgcgcaggc gctggacgcc gacgaccagc aggacatcac cgtcgccggg cggatcctgc ggatccgcga cttcggtggt gtgctgttcg cccagctgcg ggactggtcg ggggaaatgc aagtgctgct ggacaattcg cgtctggagc ggggccgcac ggccgacttc accgcggcca tcgatctcgg cgacctggtc gaggtgtccg gtcagatggg gttctccaag aaaggcaccc ggtcgctgat cgtcaccgac tggcggatga tcggtaagtg cttgcggcca ttgccgaata agtggaaggg gctcaccgat ccggaggccc gggtgcgaac ccgctacgtc gacctggcgg tcaaccccga gtcgcgggag ttgatcgcgg cgcgcagcga ggtgctgcgc tcggtccggc agaccctgtt cgccaaggga ttcatcgagg tcgagacccc gatcctgcag cagatccacg gcggcgccac cgcccggccg ttcgtcacgc acatcaacac ctacgacatg gacctgttcc tgcgcatcgc gccggagctg tatctgaagc ggctgtgcgt cggcggcgtg gagcgcgtct tcgagttggg ccgggcgttc cgcaacgagg gggtcgactt cagccacaac ccggagttca ccctgctgga ggcctaccag gcgcacgccg actatcgggt gtggatcgac gggtgccgcg agctgatcca gaacgccgcc caggccgcgc acggcgagca gaccgtgctg cggcccggcg ccgacggccg gctgcaaccg gtcgacatct ccgggatctg ggcggtcaag accgtgcacg acgcggtgtc cgaggcactc ggcgagcagg tcgaccccgg cacgtcgctg tccaccctgc gcaaactgtc cgacgcggcg cgcatcccct accgcgcgca ctgggacgcc ggcgcggtgg tgctcgagct ctacgagcac ctggtcgagg accggaccga ggagccgacg ttctacgtcg acttccccac ctcggtgtcg ccgctgaccc ggccgcaccg cagccggccc ggggtcgcgg agcggtggga cctggtggcc tggggtgtcg agctggccac cgcctacagc gagctgaccg atccggtgga gcagcgccgc cgcctgcagg agcagtcgct gttagctgcc ggcggggatc ccgaggcgat ggaactcgac gaggacttcc tgcaggcgat ggagtacgcg atgccgccga cgggcgggct gggcatgggc gtcgatcgcc tcgtcatgct cataacgggc cgcagcatcc gcgaaacctt gccgtttccg ctggccaagc cgcactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat