MypaA.19272.a

QcrA

CENTER ID: MypaA.19272.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MypaA.19272.a.B2.GE40301 Rieske iron-sulfur domain 234 422
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|262316.17.peg.2051
UniProt: Q73YM1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGSPPAAQRR RGRTEEEERR KMSDGDVRGS DTDKGAGAPN EPDEAALAAM SQQELVTLGG KLDGVETVFK EPRWPVEDTK AEKRAERGVA LWLLLGGGFG LALLLIFLFW PWEYKPKEAA GSFLYDLATP LYGLTFGMSI LSIGIGTILY QKRFIPEEIS IQQRHDGASR EIDRKTVVAN LADAYNGSTI GRRKMVGLSL GVGLGAFGLS TLVAFAGGLI KNPWKPVVPT ANGKKAVLWT SGWTPRYPGE TIYLARATGT YTGAPFVKMR PEDLDAGGME TVFPWRESDG DGTTPESQEK LRAINQGVRN PVMLIRIRPT DMPRVVKRQG QESFNFGEFF AYTKVCSHLG CPASLYEEQS YRILCPCHQS QFDALHFAKP IFGPAARALA QLPITIDTNG YLVANGDFVE PVGPAFWERT TT
NT Sequence
gtggggtctc ccccagccgc gcaacggcga aggggccgaa ccgaagagga ggagcggcgc aaaatgagcg acggcgacgt tcgcggctct gacaccgaca agggagccgg ggcgcccaac gagcccgacg aagcggcgct ggccgcgatg tcgcagcagg agctggtgac gctgggcggc aagctcgacg gcgtcgaaac ggtgttcaag gaaccccggt ggccggtaga ggacaccaag gccgagaagc gcgccgagcg cggcgtcgcc ctgtggcttt tgctgggcgg cggcttcggg ctggcgctgc tgctgatctt cttgttctgg ccgtgggagt acaagcccaa ggaggccgcc ggcagcttcc tgtacgacct ggccaccccg ctgtacggcc tgaccttcgg gatgtccatc ctgtcgatcg ggatcggcac catcctgtac cagaaacgct ttatcccaga agagatttcg atccagcagc ggcacgacgg cgcctcccgc gagatcgacc gcaagacggt ggtggcgaac ctggccgacg cctacaacgg gtccaccatc gggcggcgca agatggtcgg gctgtcgttg ggcgtgggcc tgggggcgtt cggcctgtcc accctggtcg cgtttgccgg gggcctgatc aagaacccgt ggaagccggt ggtgcccacc gccaacggga agaaagccgt gctgtggacg tcgggctgga ccccgcgcta cccgggcgag accatctacc tggcccgcgc caccggcacc tacaccgggg ccccgttcgt caagatgcgg cccgaggacc tcgacgccgg cggcatggaa accgtgttcc cgtggcgcga gtccgacggc gacggcacca ccccggaatc gcaggagaag ctgcgggcga tcaaccaggg tgtgcgaaac ccggtgatgc tcatcaggat tcggcccacc gacatgcccc gcgtggtcaa gcggcagggc caggagagct tcaacttcgg cgagttcttc gcctacacca aggtgtgctc gcatctgggt tgccccgcat cgctgtacga ggagcagtcc taccggatcc tgtgcccctg ccaccagtcg cagttcgacg cgctgcattt cgccaagccg atcttcgggc cggccgcgcg ggcgttggcg caactgccga tcaccatcga caccaacggg tatctggtcg ccaacggtga cttcgtcgaa cccgtcggac cggcattctg ggagaggacg acaacatga
Details for MypaA.19272.a.B2.GE40301
HARVESTED ON: 6/13/2016
SEQUENCED ON: 6/16/2016
EXPECTED MW: 22kDa
OBSERVED MW: 22kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHKK AVLWTSGWTP RYPGETIYLA RATGTYTGAP FVKMRPEDLD AGGMETVFPW RESDGDGTTP ESQEKLRAIN QGVRNPVMLI RIRPTDMPRV VKRQGQESFN FGEFFAYTKV CSHLGCPASL YEEQSYRILC PCHQSQFDAL HFAKPIFGPA ARALAQLPIT IDTNGYLVAN GDFVEPVGPA FWERTTT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataagaaag ccgtgctgtg gacgtcgggc tggaccccgc gctacccggg cgagaccatc tacctggccc gcgccaccgg cacctacacc ggggccccgt tcgtcaagat gcggcccgag gacctcgacg ccggcggcat ggaaaccgtg ttcccgtggc gcgagtccga cggcgacggc accaccccgg aatcgcagga gaagctgcgg gcgatcaacc agggtgtgcg aaacccggtg atgctcatca ggattcggcc caccgacatg ccccgcgtgg tcaagcggca gggccaggag agcttcaact tcggcgagtt cttcgcctac accaaggtgt gctcgcatct gggttgcccc gcatcgctgt acgaggagca gtcctaccgg atcctgtgcc cctgccacca gtcgcagttc gacgcgctgc atttcgccaa gccgatcttc gggccggccg cgcgggcgtt ggcgcaactg ccgatcacca tcgacaccaa cgggtatctg gtcgccaacg gtgacttcgt cgaacccgtc ggaccggcat tctgggagag gacgacaaca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat