MypaA.18665.a

Uncharacterized protein

CENTER ID: MypaA.18665.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.18665.a.MB1.PS02418 full length 1 452

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.4390
RefSeq: NP_963053.1
UniProt: Q73SF5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSTKHREVA KLDRVPLPVE AARVAATGWQ VTRSAARVLT KLPAKGPWQQ KVIKQLPQTF ADLGPTYVKF GQIIASSPGA FGESLSREFR GLLDRVPPAD PDEVHKLFLE ELGADPSELF ASWDETPFAS ASIAQVHYAT LHTGEEVVVK IQRPGIRRRV AADLQILKRF AQAVELAKLG RRLSAQDVVA DFSDNLAEEL NFRLEAQSME AWVSHLHASP LGKNIRVPQV YWDFTTDRVL TMERVSGIRI DDVAAVRKAG FDGVELVKAL LFSLFEGGLR HGLFHGDLHA GNLYVDEAGR IVFFDFGIMG RIDPRTRWLL RELVYALLVK KDHAAAGKIV VLMGAVGTVK PEAQAAKDLE KFATPLTMQT LGDMSYADIG RQLSALADAY DVKLPRELVL IGKQFLYVER YMKLLAPKWQ MMSDPQLTGY FANFMVEVSR EHSSEFESDV EV
NT Sequence
atgagctcta ccaaacatcg agaggtggcc aagctcgacc gggtgccgtt gccggtcgag gcggcccggg tagcggcgac cggttggcag gtcacccgct ccgccgctcg cgtcctcacc aagctgccgg ccaagggccc gtggcagcag aaagtgatca agcagctccc ccagacgttc gccgacctgg ggcccaccta cgtcaagttc gggcagatca tcgcgtccag ccccggcgcg ttcggcgaat cgctgtcccg ggagttccgc ggcctgttgg accgggtgcc gcccgccgac cccgacgagg tgcacaagct gttcctcgag gagctcgggg ccgacccgtc cgagctgttc gccagctggg acgagacccc gttcgcctcg gcgtccatcg cccaggtgca ctacgccacc ctgcacaccg gcgaggaggt cgtcgtcaag atccagcggc cgggcatccg ccgccgggtc gccgccgacc tgcagatcct caagcgcttc gcccaggccg tcgagctggc caagctcggc cgccggctgt ccgcccagga cgtggtcgcc gacttctccg acaacctggc cgaggagctg aacttccggc tggaggccca gtcgatggag gcctgggtgt cgcacctgca cgcctcgccg ctgggcaaaa acatccgggt gccgcaggtg tactgggact tcaccaccga ccgggtgctg accatggagc gggtgtccgg gatccgcatc gacgacgtcg ccgcggtccg caaggccggg ttcgacgggg ttgagctggt caaggcgctg ctgttcagcc tgttcgaggg cgggctgcgg cacggcctgt tccacggcga cctgcacgcc ggcaacctct acgtcgacga ggcgggccgc atcgtgttct tcgacttcgg catcatgggc cgcatcgacc cgcgcacccg ctggctgctg cgcgagctcg tgtatgcgct gctggtcaag aaggaccacg ccgcggccgg caagatcgtg gtgctgatgg gcgccgtggg caccgtgaaa cccgaagccc aggccgccaa ggacctggag aaattcgcca ccccgctgac catgcagacc ctgggcgaca tgtcctatgc cgacatcggc cgccagctgt cggcgctggc cgacgcctac gacgtcaagc tgccccgcga gctggtgctg atcggcaagc agttcctcta cgtcgagcgc tacatgaagc tgctggcacc caagtggcag atgatgtcgg atcctcaact gaccgggtat ttcgccaact tcatggtcga ggtcagccgc gagcactcct ccgagttcga atccgacgtc gaggtctag
Details for MypaA.18665.a.MB1.PS02418
PURIFICATION DATe: 6/10/2015
CONCENTRATION: 26.31mg/ml
OBSERVED MW: 98kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MSSTKHREVA KLDRVPLPVE AARVAATGWQ VTRSAARVLT KLPAKGPWQQ KVIKQLPQTF ADLGPTYVKF GQIIASSPGA FGESLSREFR GLLDRVPPAD PDEVHKLFLE ELGADPSELF ASWDETPFAS ASIAQVHYAT LHTGEEVVVK IQRPGIRRRV AADLQILKRF AQAVELAKLG RRLSAQDVVA DFSDNLAEEL NFRLEAQSME AWVSHLHASP LGKNIRVPQV YWDFTTDRVL TMERVSGIRI DDVAAVRKAG FDGVELVKAL LFSLFEGGLR HGLFHGDLHA GNLYVDEAGR IVFFDFGIMG RIDPRTRWLL RELVYALLVK KDHAAAGKIV VLMGAVGTVK PEAQAAKDLE KFATPLTMQT LGDMSYADIG RQLSALADAY DVKLPRELVL IGKQFLYVER YMKLLAPKWQ MMSDPQLTGY FANFMVEVSR EHSSEFESDV EV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCACA TGAAAATCGA AGAAGGTAAA CTGGTAATCT GGATTAACGG CGATAAAGGC TATAACGGTC TCGCTGAAGT CGGTAAGAAA TTCGAGAAAG ATACCGGAAT TAAAGTCACC GTTGAGCATC CGGATAAACT GGAAGAGAAA TTCCCACAGG TTGCGGCAAC TGGCGATGGC CCTGACATTA TCTTCTGGGC ACACGACCGC TTTGGTGGCT ACGCTCAATC TGGCCTGTTG GCTGAAATCA CCCCGGACAA AGCGTTCCAG GACAAGCTGT ATCCGTTTAC CTGGGATGCC GTACGTTACA ACGGCAAGCT GATTGCTTAC CCGATCGCTG TTGAAGCGTT ATCGCTGATT TATAACAAAG ATCTGCTGCC GAACCCGCCA AAAACCTGGG AAGAGATCCC GGCGCTGGAT AAAGAACTGA AAGCGAAAGG TAAGAGCGCG CTGATGTTCA ACCTGCAAGA ACCGTACTTC ACCTGGCCGC TGATTGCTGC TGACGGGGGT TATGCGTTCA AGTATGAAAA CGGCAAGTAC GACATTAAAG ACGTGGGCGT GGATAACGCT GGCGCGAAAG CGGGTCTGAC CTTCCTGGTT GACCTGATTA AAAACAAACA CATGAATGCA GACACCGATT ACTCCATCGC AGAAGCTGCC TTTAATAAAG GCGAAACAGC GATGACCATC AACGGCCCGT GGGCATGGTC CAACATCGAC ACCAGCAAAG TGAATTATGG TGTAACGGTA CTGCCGACCT TCAAGGGTCA ACCATCCAAA CCGTTCGTTG GCGTGCTGAG CGCAGGTATT AACGCCGCCA GTCCGAACAA AGAGCTGGCA AAAGAGTTCC TCGAAAACTA TCTGCTGACT GATGAAGGTC TGGAAGCGGT TAATAAAGAC AAACCGCTGG GTGCCGTAGC GCTGAAGTCT TACGAGGAAG AGTTGGCGAA AGATCCACGT ATTGCCGCCA CTATGGAAAA CGCCCAGAAA GGTGAAATCA TGCCGAACAT CCCGCAGATG TCCGCTTTCT GGTATGCCGT GCGTACTGCG GTGATCAACG CCGCCAGCGG TCGTCAGACT GTCGATGAAG CCCTGAAAGA CGCGCAGACT AATTCGAGCT CGAACAACAA CAACAATAAC AATAACAACA ACCTCGGGAT CGAGGGAAGG ATTTCAGAAG GTACCCTGGA AGCTCAGACC CAGGGTCCTG GTTCGATGAG CTCTACCAAA CATCGAGAGG TGGCCAAGCT CGACCGGGTG CCGTTGCCGG TCGAGGCGGC CCGGGTAGCG GCGACCGGTT GGCAGGTCAC CCGCTCCGCC GCTCGCGTCC TCACCAAGCT GCCGGCCAAG GGCCCGTGGC AGCAGAAAGT GATCAAGCAG CTCCCCCAGA CGTTCGCCGA CCTGGGGCCC ACCTACGTCA AGTTCGGGCA GATCATCGCG TCCAGCCCCG GCGCGTTCGG CGAATCGCTG TCCCGGGAGT TCCGCGGCCT GTTGGACCGG GTGCCGCCCG CCGACCCCGA CGAGGTGCAC AAGCTGTTCC TCGAGGAGCT CGGGGCCGAC CCGTCCGAGC TGTTCGCCAG CTGGGACGAG ACCCCGTTCG CCTCGGCGTC CATCGCCCAG GTGCACTACG CCACCCTGCA CACCGGCGAG GAGGTCGTCG TCAAGATCCA GCGGCCGGGC ATCCGCCGCC GGGTCGCCGC CGACCTGCAG ATCCTCAAGC GCTTCGCCCA GGCCGTCGAG CTGGCCAAGC TCGGCCGCCG GCTGTCCGCC CAGGACGTGG TCGCCGACTT CTCCGACAAC CTGGCCGAGG AGCTGAACTT CCGGCTGGAG GCCCAGTCGA TGGAGGCCTG GGTGTCGCAC CTGCACGCCT CGCCGCTGGG CAAAAACATC CGGGTGCCGC AGGTGTACTG GGACTTCACC ACCGACCGGG TGCTGACCAT GGAGCGGGTG TCCGGGATCC GCATCGACGA CGTCGCCGCG GTCCGCAAGG CCGGGTTCGA CGGGGTTGAG CTGGTCAAGG CGCTGCTGTT CAGCCTGTTC GAGGGCGGGC TGCGGCACGG CCTGTTCCAC GGCGACCTGC ACGCCGGCAA CCTCTACGTC GACGAGGCGG GCCGCATCGT GTTCTTCGAC TTCGGCATCA TGGGCCGCAT CGACCCGCGC ACCCGCTGGC TGCTGCGCGA GCTCGTGTAT GCGCTGCTGG TCAAGAAGGA CCACGCCGCG GCCGGCAAGA TCGTGGTGCT GATGGGCGCC GTGGGCACCG TGAAACCCGA AGCCCAGGCC GCCAAGGACC TGGAGAAATT CGCCACCCCG CTGACCATGC AGACCCTGGG CGACATGTCC TATGCCGACA TCGGCCGCCA GCTGTCGGCG CTGGCCGACG CCTACGACGT CAAGCTGCCC CGCGAGCTGG TGCTGATCGG CAAGCAGTTC CTCTACGTCG AGCGCTACAT GAAGCTGCTG GCACCCAAGT GGCAGATGAT GTCGGATCCT CAACTGACCG GGTATTTCGC CAACTTCATG GTCGAGGTCA GCCGCGAGCA CTCCTCCGAG TTCGAATCCG ACGTCGAGGT CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA