SSGCID: Target Page

MypaA.17943.a

Leucine--tRNA ligase (EC 6.1.1.4) (Leucyl-tRNA synthetase) (LeuRS)

CENTER ID:	MypaA.17943.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.17943.a.B1.GE42456	full length		1	969

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.52
UniProt:	Q744V3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTESPTTSPA TGSGAAAPDS DAPPYRYTAA LAGRIEGSWQ DTWAKLGTFN VPNPVGSLAP TDGTPVPEDK LFVQDMFPYP SGEGLHVGHP LGYIATDVYA RYFRMTGRNV LHALGFDAFG LPAEQYAVQT GTHPRTRTEA NVVNFRRQLG RLGLGHDSRR SFSTTDVEFY KWTQWIFLQI YNAWFDPAAN KARPIAELVA EFDSGARSLD DGRNWSELSA GERADVIDSH RLVYRADSMV NWCPGLGTVL ANEEVTADGR SDRGNFPVFR KRLRQWMMRI TAYSDRLLDD LDLLDWPEPV KTMQRNWIGR STGAKALFAA TGADGAALDI EVFTTRPDTL FGATYMVLAP EHELVDELVA PAWPDGTDPR WTYGAATPGE SVAAYRRAIA SKSDLERQES KAKTGVFLGS YATNPTNGKP VPIFIADYVL AGYGTGAIMA VPGHDQRDWD FAHEFGLPIV EVIAGGDISE GAYAGDGVLV NSGYLDGLDV AAAKEAITAR LEAEGRGCAR VEYKLRDWLF ARQRYWGEPF PIVYDSDGRP HALDEAALPV ELPDVPDYSP VLFDPDDADS EPSPPLAKAT DWVHVELDLG DGLKPYSRDT NVMPQWAGSS WYELRYTDPH NSERFCAKEN EAYWMGPRPA EHGPQDPGGV DLYVGGAEHA VLHLLYARFW HKVLYDLGHV SSREPYRRLV NQGYIQAFAY TDSRGSYVPA EEVVERDGRF FYRGPDGEIE VFQEFGKIGK SLKNSISPDE ICDDYGADTL RVYEMSMGPL EASRPWATKD VVGAHRFLQR VWRLVVDEQT GETRVVDGAG RDLPTGTLRL LHRTIAGVSE DYAALRNNTA VAKLIEYTNH LTKEHRDAVP RAAVEPLVLM LAPLAPHMAE ELWLRLGHTT SLAHGPFPVA DPAYLVEDTV EYPVQVNGKV RGRVTVAADA DRDTLEAAAL ADEKVLAFLA GAQPRKVIVV PGRLVNLVV

NT Sequence

gtgaccgaat ccccgaccac ttcgcccgcc accggctccg gtgccgccgc gccggactcc gacgcgccgc cgtatcgcta caccgcggcg ctggcgggcc ggatcgaggg cagctggcag gacacctggg ccaagctcgg gacgttcaac gtgcccaacc cggtcggttc gttggccccg acggacggga ccccggtccc cgaggacaag ctgttcgtgc aggacatgtt cccctacccg tcgggggagg ggctgcacgt cggccacccg ctgggctaca tcgccaccga cgtgtacgcc cgctacttcc ggatgaccgg ccgtaacgtg ttgcatgcgt tgggttttga cgctttcggc ctgcccgccg agcaatacgc ggtgcagacc ggcacccacc cgcgcaccag gaccgaggcc aacgtggtca acttccgccg ccagctgggc cggctggggc tgggccacga cagcaggcgc agcttctcca ccaccgacgt cgagttctac aagtggacgc agtggatctt cctgcagatc tacaacgcct ggttcgaccc cgccgccaac aaggcccgac ccatcgccga gctggtcgcc gaattcgatt ccggcgcgcg cagcctcgac gacgggcgca actggtcgga gttgtcggcg ggggagcggg ccgacgtgat cgacagccac cggctggtct accgtgcgga ctcgatggtc aactggtgtc ccggactggg caccgtgctg gccaacgagg aggtcaccgc cgacgggcgc agcgaccgcg gcaacttccc ggtgttccga aagcggttgc ggcagtggat gatgcggatc accgcctact ccgatcggct gctcgacgac ctcgacctgc tggactggcc ggagccggtg aagaccatgc agcgcaactg gatcggccgt tccaccggcg ccaaggccct gttcgcggcg accggcgccg acggcgcggc gctcgacatc gaggtgttta ccacccgacc ggacacgctg ttcggcgcca cctatatggt gctggccccc gagcacgagc tggtcgacga gctggtcgcg cccgcctggc cggacggcac cgacccgcgc tggacatacg gcgccgccac tccgggcgag tcggtcgccg cctaccggcg ggcgatcgcc tcgaagtcgg acctcgagcg ccaggagagc aaggccaaaa ccggtgtctt cctgggcagc tacgccacca atcccaccaa tggcaagccg gttccgatct tcatcgccga ctacgtgctg gccggatacg gcaccggcgc gatcatggcg gtgcccggcc acgaccagcg cgactgggac ttcgcgcacg aattcggcct gcccatcgtg gaagtcattg ccggaggcga tatttcggag ggcgcctacg ccggcgacgg ggtgttggtg aactccggct acctggacgg actggatgtg gcggcggcca aggaggcgat caccgcccgg ctggaagccg aaggccgtgg ctgcgcgcgg gtcgaataca agttgcgtga ctggcttttc gcccggcaac ggtactgggg cgagccgttc ccgatcgtct acgacagcga cgggcggccg catgccctcg acgaagccgc tctgccggtg gaactgcccg acgtgccgga ctattcgccg gtgctgttcg accccgacga cgccgacagc gaaccgtcgc cgccgttggc caaggcgacc gactgggtgc acgtcgagct ggacctcggc gacggcctga agccctacag ccgcgacacc aacgtgatgc cgcaatgggc cggcagctcc tggtacgaac tgcgctacac cgatccgcac aactcggaac gcttctgcgc caaggagaat gaggcctact ggatggggcc gcggcccgcc gagcacgggc cgcaggatcc cggcggcgtc gacctgtacg tcggtggggc cgaacacgcg gtgttgcatc tgctctacgc ccggttctgg cacaaggtgc tctacgacct gggccacgtc agctcccgcg agccgtaccg ccggctggtc aaccagggct acattcaggc gttcgcctac accgactcgc gcggctcgta cgtgccggcc gaggaggtcg tcgaacgcga cggccggttt ttctaccggg gtcccgacgg ggagatcgag gtcttccagg aattcgggaa aatcgggaag agcctgaaga attcgatctc acccgacgag atctgcgacg actacggcgc cgacacgctg cgggtctacg agatgtcgat gggtccgctg gaggcgtccc gtccctgggc gaccaaggat gtggtgggcg ctcaccggtt cctgcagcgg gtgtggcgac tggtcgtcga cgagcagacg ggcgagacgc gggtggtcga cggggccgga cgggatctgc ccaccggtac cctgcggctg ctgcaccgca ccattgccgg agtctcggaa gactatgcgg ccctgcgcaa taacaccgcc gtggccaagt tgatcgagta caccaaccat ctcaccaagg aacatcgcga cgcggtgccc cgcgcggcgg tcgagccgct ggtgttgatg ctggcgccgt tggcgccgca catggccgag gagttgtggc tgcggctggg ccacaccacc tcgctggcgc acggcccgtt cccggtggcg gacccggcgt acctcgtcga ggacaccgtc gaatacccgg tgcaggtgaa cggcaaggtg cgcgggaggg tgacggtggc ggccgacgcc gatcgggaca ccctcgaggc cgccgcgctg gccgacgaga aggtgctggc gttcctggcc ggcgcccagc cgcgcaaggt gatcgtggtc ccgggccggc tggtcaacct ggtggtgtag

Details for MypaA.17943.a.B1.GE42456

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	108kDa
OBSERVED MW:	108kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT ESPTTSPATG SGAAAPDSDA PPYRYTAALA GRIEGSWQDT WAKLGTFNVP NPVGSLAPTD GTPVPEDKLF VQDMFPYPSG EGLHVGHPLG YIATDVYARY FRMTGRNVLH ALGFDAFGLP AEQYAVQTGT HPRTRTEANV VNFRRQLGRL GLGHDSRRSF STTDVEFYKW TQWIFLQIYN AWFDPAANKA RPIAELVAEF DSGARSLDDG RNWSELSAGE RADVIDSHRL VYRADSMVNW CPGLGTVLAN EEVTADGRSD RGNFPVFRKR LRQWMMRITA YSDRLLDDLD LLDWPEPVKT MQRNWIGRST GAKALFAATG ADGAALDIEV FTTRPDTLFG ATYMVLAPEH ELVDELVAPA WPDGTDPRWT YGAATPGESV AAYRRAIASK SDLERQESKA KTGVFLGSYA TNPTNGKPVP IFIADYVLAG YGTGAIMAVP GHDQRDWDFA HEFGLPIVEV IAGGDISEGA YAGDGVLVNS GYLDGLDVAA AKEAITARLE AEGRGCARVE YKLRDWLFAR QRYWGEPFPI VYDSDGRPHA LDEAALPVEL PDVPDYSPVL FDPDDADSEP SPPLAKATDW VHVELDLGDG LKPYSRDTNV MPQWAGSSWY ELRYTDPHNS ERFCAKENEA YWMGPRPAEH GPQDPGGVDL YVGGAEHAVL HLLYARFWHK VLYDLGHVSS REPYRRLVNQ GYIQAFAYTD SRGSYVPAEE VVERDGRFFY RGPDGEIEVF QEFGKIGKSL KNSISPDEIC DDYGADTLRV YEMSMGPLEA SRPWATKDVV GAHRFLQRVW RLVVDEQTGE TRVVDGAGRD LPTGTLRLLH RTIAGVSEDY AALRNNTAVA KLIEYTNHLT KEHRDAVPRA AVEPLVLMLA PLAPHMAEEL WLRLGHTTSL AHGPFPVADP AYLVEDTVEY PVQVNGKVRG RVTVAADADR DTLEAAALAD EKVLAFLAGA QPRKVIVVPG RLVNLVV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaccg aatccccgac cacttcgccc gccaccggct ccggtgccgc cgcgccggac tccgacgcgc cgccgtatcg ctacaccgcg gcgctggcgg gccggatcga gggcagctgg caggacacct gggccaagct cgggacgttc aacgtgccca acccggtcgg ttcgttggcc ccgacggacg ggaccccggt ccccgaggac aagctgttcg tgcaggacat gttcccctac ccgtcggggg aggggctgca cgtcggccac ccgctgggct acatcgccac cgacgtgtac gcccgctact tccggatgac cggccgtaac gtgttgcatg cgttgggttt tgacgctttc ggcctgcccg ccgagcaata cgcggtgcag accggcaccc acccgcgcac caggaccgag gccaacgtgg tcaacttccg ccgccagctg ggccggctgg ggctgggcca cgacagcagg cgcagcttct ccaccaccga cgtcgagttc tacaagtgga cgcagtggat cttcctgcag atctacaacg cctggttcga ccccgccgcc aacaaggccc gacccatcgc cgagctggtc gccgaattcg attccggcgc gcgcagcctc gacgacgggc gcaactggtc ggagttgtcg gcgggggagc gggccgacgt gatcgacagc caccggctgg tctaccgtgc ggactcgatg gtcaactggt gtcccggact gggcaccgtg ctggccaacg aggaggtcac cgccgacggg cgcagcgacc gcggcaactt cccggtgttc cgaaagcggt tgcggcagtg gatgatgcgg atcaccgcct actccgatcg gctgctcgac gacctcgacc tgctggactg gccggagccg gtgaagacca tgcagcgcaa ctggatcggc cgttccaccg gcgccaaggc cctgttcgcg gcgaccggcg ccgacggcgc ggcgctcgac atcgaggtgt ttaccacccg accggacacg ctgttcggcg ccacctatat ggtgctggcc cccgagcacg agctggtcga cgagctggtc gcgcccgcct ggccggacgg caccgacccg cgctggacat acggcgccgc cactccgggc gagtcggtcg ccgcctaccg gcgggcgatc gcctcgaagt cggacctcga gcgccaggag agcaaggcca aaaccggtgt cttcctgggc agctacgcca ccaatcccac caatggcaag ccggttccga tcttcatcgc cgactacgtg ctggccggat acggcaccgg cgcgatcatg gcggtgcccg gccacgacca gcgcgactgg gacttcgcgc acgaattcgg cctgcccatc gtggaagtca ttgccggagg cgatatttcg gagggcgcct acgccggcga cggggtgttg gtgaactccg gctacctgga cggactggat gtggcggcgg ccaaggaggc gatcaccgcc cggctggaag ccgaaggccg tggctgcgcg cgggtcgaat acaagttgcg tgactggctt ttcgcccggc aacggtactg gggcgagccg ttcccgatcg tctacgacag cgacgggcgg ccgcatgccc tcgacgaagc cgctctgccg gtggaactgc ccgacgtgcc ggactattcg ccggtgctgt tcgaccccga cgacgccgac agcgaaccgt cgccgccgtt ggccaaggcg accgactggg tgcacgtcga gctggacctc ggcgacggcc tgaagcccta cagccgcgac accaacgtga tgccgcaatg ggccggcagc tcctggtacg aactgcgcta caccgatccg cacaactcgg aacgcttctg cgccaaggag aatgaggcct actggatggg gccgcggccc gccgagcacg ggccgcagga tcccggcggc gtcgacctgt acgtcggtgg ggccgaacac gcggtgttgc atctgctcta cgcccggttc tggcacaagg tgctctacga cctgggccac gtcagctccc gcgagccgta ccgccggctg gtcaaccagg gctacattca ggcgttcgcc tacaccgact cgcgcggctc gtacgtgccg gccgaggagg tcgtcgaacg cgacggccgg tttttctacc ggggtcccga cggggagatc gaggtcttcc aggaattcgg gaaaatcggg aagagcctga agaattcgat ctcacccgac gagatctgcg acgactacgg cgccgacacg ctgcgggtct acgagatgtc gatgggtccg ctggaggcgt cccgtccctg ggcgaccaag gatgtggtgg gcgctcaccg gttcctgcag cgggtgtggc gactggtcgt cgacgagcag acgggcgaga cgcgggtggt cgacggggcc ggacgggatc tgcccaccgg taccctgcgg ctgctgcacc gcaccattgc cggagtctcg gaagactatg cggccctgcg caataacacc gccgtggcca agttgatcga gtacaccaac catctcacca aggaacatcg cgacgcggtg ccccgcgcgg cggtcgagcc gctggtgttg atgctggcgc cgttggcgcc gcacatggcc gaggagttgt ggctgcggct gggccacacc acctcgctgg cgcacggccc gttcccggtg gcggacccgg cgtacctcgt cgaggacacc gtcgaatacc cggtgcaggt gaacggcaag gtgcgcggga gggtgacggt ggcggccgac gccgatcggg acaccctcga ggccgccgcg ctggccgacg agaaggtgct ggcgttcctg gccggcgccc agccgcgcaa ggtgatcgtg gtcccgggcc ggctggtcaa cctggtggtg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat