MypaA.11416.c

ParB (Rv3918c ortholog)

CENTER ID: MypaA.11416.c
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.11416.c.B1.GE37480 full length 1 367
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.4622
RefSeq: NP_963278.1
UniProt: Q73RT4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSSGDWPVA SSEAREGAAT PAPDAPSATH TPAAPIGPGA PSGTRGASAV GSPAPPPTAA TAPPSSAAAS TSPPANVSRE TADEFDTPIG AAAERAMRIL HTTYPPLRRP KHRRVFTVAN QKGGVGKTTT AVNLAAALAL QGLKTLVIDL DPQGNASTAL GITDRQSGTP SSYEVLLGEV SVHDALRQSP HNERLFCIPA TIDLAGAEIE LVSMVARENR LRTALADLDN LDFDCVFIDC PPSLGLLTIN ALVAAPEVMI PIQCEYYALE GVSQLMRNIE MVKAHLNPQL EVSTVVLTMY DGRTKLADQV AEEVRRYFGS KVLRTVIPRS VKVSEAPGYS MTIIDYDPGS RGAMSYLDAS RELAERD
NT Sequence
atgacttctt caggagattg gccggtggct tcttcggagg cgcgggaggg cgcggcgacg cccgcgcccg acgcaccgag cgcgacgcac accccggcgg cacccattgg gccgggggcc ccgtcgggca cgcggggggc gtcggcggtt ggatccccgg cgccgccgcc gaccgcggcc acggcgccgc cgtcatccgc cgccgcgtcg acctcgccgc cggccaatgt ttcacgtgaa acagcggacg aattcgacac cccgatcggc gcggcggccg agcgcgcgat gcggatcctc cacaccacct atccgccgct acgccggccc aagcatcgcc gcgtcttcac cgtcgcgaac cagaagggcg gcgtgggcaa gacgacgacc gccgtcaacc tcgccgctgc gctcgccctg cagggactca aaaccctcgt catcgacctc gacccgcagg gcaacgccag caccgcgctc gggatcacgg atcggcagtc gggtacgccg tcgtcctacg aggtcctgct cggcgaggtg tcggtgcacg acgcgttgcg gcagagcccg cacaacgagc gactgttctg catcccggcg accatcgacc tggccggcgc cgagatcgag ttggtgagca tggtggcgcg ggagaaccgg ctgcggaccg cgctggccga tctggacaac ctcgacttcg actgcgtctt catcgactgc ccaccgtcgc tcgggctgct gaccatcaac gcgctcgtcg ccgccccgga ggtgatgatc ccgatccagt gcgagtacta cgccctcgag ggcgtgtcgc agctgatgcg caacatcgag atggtgaagg cgcacctcaa cccgcagctc gaagtgagca ccgtggtgct gaccatgtac gacggccgca ccaagctcgc ggaccaggtg gccgaagagg tgcgccgcta cttcggcagc aaggtgctgc ggacggtgat cccgcgcagc gtcaaggtgt ccgaggcgcc cggctacagc atgaccatca tcgactacga ccccgggtcg cgcggggcga tgagctatct cgatgcgagc cgcgaactcg ccgagcgtga ttga
Details for MypaA.11416.c.B1.GE37480
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 40kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 72
Validated AA Sequence
MRXLHTTYPP XRRPKHRRVF TVANQKGGVG KTTTAVNLAA ALALQGLKTL VIDLDPQGNA STALGITDRQ SGTPSSYEVL LGEVSVHDAL RQSPHNERLF CIPATIDLAG AEIELVSMVA RENRLRTALA DLDNLDFDCV FIDCPPSLGL LTINALVAAP EVMIPIQCEY YALEGVSQLM RNIEMVKAHL NPQLEVSTVV LTMYDGRTKL ADQVAEEVRR YFGSKVLRTV IPRSVKVSEA PGYSMTIIDY DPGSRGAMSY LDASRELAER D
Validated NT Sequence
atcttactca cttaatcacg ctcggcgagt tcgcggctcg catcgagata gctcatcgcc ccgcgcgacc cggggtcgta gtcgatgatg gtcatgctgt agccgggcgc ctcggacacc ttgacgctgc gcgggatcac cgtccgcagc accttgctgc cgaagtagcg gcgcacctct tcggccacct ggtccgcgag cttggtgcgg ccgtcgtaca tggtcagcac cacggtgctc acttcgagct gcgggttgag gtgcgccttc accatctcga tgttgcgcat cagctgcgac acgccctcga gggcgtagta ctcgcactgg atcgggatca tcacctccgg ggcggcgacg agcgcgttga tggtcagcag cccgagcgac ggtgggcagt cgatgaagac gcagtcgaag tcgaggttgt ccagatcggc cagcgcggtc cgcagccggt tctcccgcgc caccatgctc accaactcga tctcggcgcc ggccaggtcg atggtcgccg ggatgcagaa cagtcgctcg ttgtgcgggc tctgccgcaa cgcgtcgtgc accgacacct cgccgagcag gacctcgtag gacgacggcg tacccgactg ccgatccgtg atcccgagcg cggtgctggc gttgccctgc gggtcgaggt cgatgacgag ggttttgagt ccctgcaggg cgagcgcagc ggcgaggttg acggcggtcg tcgtcttgcc cacgccgccc ttctggttcg cgacggtgaa gacgcggcga tgcttgggcc ggcgtancgg cggatangtg gtgtggagga nncgcatcgc gcgctcggcc gccgcgccna tcggggtgtc naattcgtcc gctgtttcac gtgaaacatt ggccggcnnc naggtcnann cngcgg
Expected Protein Sequence
MAHHHHHHMT SSGDWPVASS EAREGAATPA PDAPSATHTP AAPIGPGAPS GTRGASAVGS PAPPPTAATA PPSSAAASTS PPANVSRETA DEFDTPIGAA AERAMRILHT TYPPLRRPKH RRVFTVANQK GGVGKTTTAV NLAAALALQG LKTLVIDLDP QGNASTALGI TDRQSGTPSS YEVLLGEVSV HDALRQSPHN ERLFCIPATI DLAGAEIELV SMVARENRLR TALADLDNLD FDCVFIDCPP SLGLLTINAL VAAPEVMIPI QCEYYALEGV SQLMRNIEMV KAHLNPQLEV STVVLTMYDG RTKLADQVAE EVRRYFGSKV LRTVIPRSVK VSEAPGYSMT IIDYDPGSRG AMSYLDASRE LAERD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactt cttcaggaga ttggccggtg gcttcttcgg aggcgcggga gggcgcggcg acgcccgcgc ccgacgcacc gagcgcgacg cacaccccgg cggcacccat tgggccgggg gccccgtcgg gcacgcgggg ggcgtcggcg gttggatccc cggcgccgcc gccgaccgcg gccacggcgc cgccgtcatc cgccgccgcg tcgacctcgc cgccggccaa tgtttcacgt gaaacagcgg acgaattcga caccccgatc ggcgcggcgg ccgagcgcgc gatgcggatc ctccacacca cctatccgcc gctacgccgg cccaagcatc gccgcgtctt caccgtcgcg aaccagaagg gcggcgtggg caagacgacg accgccgtca acctcgccgc tgcgctcgcc ctgcagggac tcaaaaccct cgtcatcgac ctcgacccgc agggcaacgc cagcaccgcg ctcgggatca cggatcggca gtcgggtacg ccgtcgtcct acgaggtcct gctcggcgag gtgtcggtgc acgacgcgtt gcggcagagc ccgcacaacg agcgactgtt ctgcatcccg gcgaccatcg acctggccgg cgccgagatc gagttggtga gcatggtggc gcgggagaac cggctgcgga ccgcgctggc cgatctggac aacctcgact tcgactgcgt cttcatcgac tgcccaccgt cgctcgggct gctgaccatc aacgcgctcg tcgccgcccc ggaggtgatg atcccgatcc agtgcgagta ctacgccctc gagggcgtgt cgcagctgat gcgcaacatc gagatggtga aggcgcacct caacccgcag ctcgaagtga gcaccgtggt gctgaccatg tacgacggcc gcaccaagct cgcggaccag gtggccgaag aggtgcgccg ctacttcggc agcaaggtgc tgcggacggt gatcccgcgc agcgtcaagg tgtccgaggc gcccggctac agcatgacca tcatcgacta cgaccccggg tcgcgcgggg cgatgagcta tctcgatgcg agccgcgaac tcgccgagcg tgattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat