MypaA.01626.a

Putative uncharacterized protein

CENTER ID: MypaA.01626.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01626.a.A1.GE29632 Full length( MypaA.01626.a ) 1 395
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.1058
RefSeq: NP_959941.1
UniProt: Q741T0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNPGANSPRP YRRLRHLATR ADKHSKGGTS VSDANGPTAR FGIDNTAVVK VPVHHGPISD IDVSPDGRRL LVTNYGRDTV SVIDTHTCRV ASTIAGLSEP FAVAMSAADP NYAYVSTATA AYDAIEVIDV VTNWRIATHR LAHSLSDLAV SPDGRYLYAS RNAVRGADVA VLDTTTGELE VIELAAAAGT TTACVRVSAD GRRLFIGVNG PNGGGLAIIE TRTRTDGRRV GGRSRLVGTV ELGLPVRDVA LGNDGNTAYV ASCGPVVGSV LDVVDTRAAT IVSTHKINEI TGPLTRMTLS RDGERAYLIS DDRVTVLGTR TQDVLGEVRV TKHPSALVES PDGHYLYVAD HSGVLSVARL SSGTAPAAPC SGADEDDDAT TGWLPELAPW EPVLA
NT Sequence
GTGAATCCGG GGGCCAATTC GCCGCGACCG TATCGGCGGC TACGCCACCT GGCCACCCGG GCCGACAAAC ACAGCAAAGG CGGTACCAGC GTGAGTGATG CAAACGGGCC TACGGCCCGA TTCGGGATCG ACAACACCGC GGTGGTCAAA GTCCCTGTTC ACCACGGCCC GATCAGCGAC ATCGACGTCA GCCCCGACGG GCGCCGGCTG CTGGTGACCA ACTACGGCCG CGACACCGTG TCGGTGATCG ACACCCACAC CTGCCGCGTC GCGAGCACCA TCGCCGGTCT GAGCGAACCG TTCGCGGTGG CCATGAGCGC CGCCGACCCG AACTACGCCT ATGTCAGCAC CGCCACCGCC GCCTACGACG CGATCGAAGT CATCGACGTC GTCACCAACT GGCGCATCGC CACGCATCGG CTGGCGCACA GCCTGAGCGA TCTGGCGGTC AGCCCGGACG GGCGGTACCT CTACGCCAGC CGCAACGCCG TGCGCGGCGC CGATGTGGCG GTGCTCGACA CCACCACGGG CGAGCTCGAG GTGATCGAGC TGGCCGCCGC GGCCGGGACG ACCACCGCCT GCGTGCGCGT CAGCGCCGAC GGTCGCCGGC TCTTCATCGG CGTCAACGGG CCCAACGGCG GCGGCCTGGC CATCATCGAA ACCCGCACCC GCACCGACGG CCGCCGGGTC GGCGGTCGCT CCCGCCTCGT CGGCACCGTC GAGCTCGGGC TGCCCGTCCG CGATGTGGCG CTCGGCAACG ACGGCAACAC GGCGTATGTG GCCAGCTGTG GCCCGGTGGT GGGCTCGGTG CTCGACGTCG TCGACACCCG GGCCGCCACC ATCGTCAGCA CGCACAAGAT CAACGAGATC ACCGGGCCGC TGACCCGGAT GACGCTGAGC CGCGACGGCG AGCGCGCCTA CCTGATCAGC GACGACCGCG TCACGGTGCT GGGCACCCGC ACCCAGGACG TCCTCGGTGA GGTCAGGGTG ACCAAGCACC CGTCGGCCCT GGTGGAAAGC CCCGACGGCC ACTACCTGTA CGTCGCCGAC CACTCCGGGG TGCTCAGCGT GGCCCGGCTC TCCTCGGGGA CCGCACCGGC CGCGCCGTGC AGCGGCGCCG ACGAGGACGA CGACGCGACC ACCGGTTGGC TGCCCGAGCT GGCGCCGTGG GAGCCGGTGC TGGCC
Details for MypaA.01626.a.A1.GE29632
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 76
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVNPGANSP RPYRRLRHLA TRADKHSKGG TSVSDANGPT ARFGIDNTAV VKVPVHHGPI SDIDVSPDGR RLLVTNYGRD TVSVIDTHTC RVASTIAGLS EPFAVAMSAA DPNYAYVSTA TAAYDAIEVI DVVTNWRIAT HRLAHSLSDL AVSPDGRYLY ASRNAVRGAD VAVLDTTTGE LEVIELAAAA GTTTACVRVS ADGRRLFIGV NGPNGGGLAI IETRTRTDGR RVGGRSRLVG TVXLGLPVRD VXLGNDXNTA YVXSCGPXXG SXXDVVXXRA AXXVSTXXXX XXRXXXPXXA XXXXXXXLLX XXXXXXXGXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
ttttntttan tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgaa tccgggggcc aattcgccgc gaccgtatcg gcggctacgc cacctggcca cccgggccga caaacacagc aaaggcggta ccagcgtgag tgatgcaaac gggcctacgg cccgattcgg gatcgacaac accgcggtgg tcaaagtccc tgttcaccac ggcccgatca gcgacatcga cgtcagcccc gacgggcgcc ggctgctggt gaccaactac ggccgcgaca ccgtgtcggt gatcgacacc cacacctgcc gcgtcgcgag caccatcgcc ggtctgagcg aaccgttcgc ggtggccatg agcgccgccg acccgaacta cgcctatgtc agcaccgcca ccgccgccta cgacgcgatc gaagtcatcg acgtcgtcac caactggcgc atcgccacgc atcggctggc gcacagcctg agcgatctgg cggtcagccc ggacgggcgg tacctctacg ccagccgcaa cgccgtgcgc ggcgccgatg tggcggtgct cgacaccacc acgggcgagc tcgaggtgat cgagctggcc gccgcggccg ggacgaccac cgcctgcgtg cgcgtcagcg ccgacggtcg ccggctcttc atcggcgtca acgggcccaa cggcggcggc ctggccatca tcgaaacccg cacccgcacc gacggccgcc gggtcggcgg tcgctcccgc ctcgtcggca ccgtcganct cgggctgccc gtccgcgatg tngngctcgg caacgacngc aacacggcgt atgtggncag ctgtggnccn gnggngggct cggngntcga cgtcgtcnan ncccgggcng ccnncntcgt cagcacgcan anatnannan atnnncggnn nnntganccn natgangctg annngcnnng nnnannnnnn ctactgntca gnnacnannn tnnngnnntn ggnnncnnnn nccnnnnnnn nnnnnnnnng ncnannnnnn cnntnnncnn nnnnnnnnnn nngnnnnnnn nnnnnnnnnn ncnnnnnnnn nggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngnnnnn nnnnnnnnnn nnnnnnnnnn nng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVNPGANSP RPYRRLRHLA TRADKHSKGG TSVSDANGPT ARFGIDNTAV VKVPVHHGPI SDIDVSPDGR RLLVTNYGRD TVSVIDTHTC RVASTIAGLS EPFAVAMSAA DPNYAYVSTA TAAYDAIEVI DVVTNWRIAT HRLAHSLSDL AVSPDGRYLY ASRNAVRGAD VAVLDTTTGE LEVIELAAAA GTTTACVRVS ADGRRLFIGV NGPNGGGLAI IETRTRTDGR RVGGRSRLVG TVELGLPVRD VALGNDGNTA YVASCGPVVG SVLDVVDTRA ATIVSTHKIN EITGPLTRMT LSRDGERAYL ISDDRVTVLG TRTQDVLGEV RVTKHPSALV ESPDGHYLYV ADHSGVLSVA RLSSGTAPAA PCSGADEDDD ATTGWLPELA PWEPVLA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaatccg ggggccaatt cgccgcgacc gtatcggcgg ctacgccacc tggccacccg ggccgacaaa cacagcaaag gcggtaccag cgtgagtgat gcaaacgggc ctacggcccg attcgggatc gacaacaccg cggtggtcaa agtccctgtt caccacggcc cgatcagcga catcgacgtc agccccgacg ggcgccggct gctggtgacc aactacggcc gcgacaccgt gtcggtgatc gacacccaca cctgccgcgt cgcgagcacc atcgccggtc tgagcgaacc gttcgcggtg gccatgagcg ccgccgaccc gaactacgcc tatgtcagca ccgccaccgc cgcctacgac gcgatcgaag tcatcgacgt cgtcaccaac tggcgcatcg ccacgcatcg gctggcgcac agcctgagcg atctggcggt cagcccggac gggcggtacc tctacgccag ccgcaacgcc gtgcgcggcg ccgatgtggc ggtgctcgac accaccacgg gcgagctcga ggtgatcgag ctggccgccg cggccgggac gaccaccgcc tgcgtgcgcg tcagcgccga cggtcgccgg ctcttcatcg gcgtcaacgg gcccaacggc ggcggcctgg ccatcatcga aacccgcacc cgcaccgacg gccgccgggt cggcggtcgc tcccgcctcg tcggcaccgt cgagctcggg ctgcccgtcc gcgatgtggc gctcggcaac gacggcaaca cggcgtatgt ggccagctgt ggcccggtgg tgggctcggt gctcgacgtc gtcgacaccc gggccgccac catcgtcagc acgcacaaga tcaacgagat caccgggccg ctgacccgga tgacgctgag ccgcgacggc gagcgcgcct acctgatcag cgacgaccgc gtcacggtgc tgggcacccg cacccaggac gtcctcggtg aggtcagggt gaccaagcac ccgtcggccc tggtggaaag ccccgacggc cactacctgt acgtcgccga ccactccggg gtgctcagcg tggcccggct ctcctcgggg accgcaccgg ccgcgccgtg cagcggcgcc gacgaggacg acgacgcgac caccggttgg ctgcccgagc tggcgccgtg ggagccggtg ctggccaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa