MypaA.01613.j

FadE31

CENTER ID: MypaA.01613.j
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01613.j.A1.GE29630 Full length( MypaA.01613.j ) 1 381
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.535
RefSeq: NP_959439.1
UniProt: Q743T6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLDLDEGTL AFRDEVRQFL SANAEAIPTK SYDNAEGFEQ HRRWDRVLYD AGLSVITWPK KYGGRDAPLL HWVVFEEEYF RAGAPGRASA NGTSMLAPTL FAHGTEEQRD RILPKMASGE EIWAQAWSEP ESGSDLASLR STATPTDGGW LLNGQKIWSS RAPFAERGFG LFRSDPSAER HHGLTYFMFD LKAEGVTVRP IVQLGGDTGF GEIFLDDVFV PDRDVIGTPH QGWRAAMSTS SNERGMSLRS PARFLATAER VVRLWQDRGS PAEFADRVAD GWIKAQAYRL QTFGTVTRLA AGGELGAESS VTKVFWSDLD VELHQTALDI LAADGELAGP WTEGLLFALG GPIYAGTNEI QRNIISERLL GLPREKSGSK K
NT Sequence
ATGGATCTGG ATCTGGACGA GGGCACCCTG GCGTTTCGGG ACGAGGTGCG GCAATTCCTG TCGGCCAACG CCGAGGCGAT CCCGACGAAG TCGTATGACA ACGCCGAAGG CTTTGAGCAG CACCGGCGTT GGGACCGGGT ACTCTACGAC GCCGGGCTGT CGGTGATCAC CTGGCCGAAG AAGTACGGCG GCCGGGACGC GCCGCTGCTG CACTGGGTGG TGTTCGAAGA GGAGTACTTC CGCGCCGGGG CGCCGGGCCG GGCCAGCGCC AACGGCACCT CGATGCTGGC CCCGACGCTG TTCGCGCACG GCACCGAGGA GCAACGCGAC CGCATCCTGC CGAAAATGGC TAGCGGCGAA GAGATCTGGG CGCAGGCGTG GTCGGAGCCC GAGTCCGGCA GCGACCTGGC GTCACTGCGG TCCACCGCGA CACCGACCGA CGGCGGCTGG CTGCTGAACG GACAGAAGAT CTGGAGCTCG CGGGCGCCGT TCGCCGAGCG GGGCTTCGGG CTGTTCCGGT CCGACCCCAG CGCCGAGCGG CACCATGGCC TGACGTATTT CATGTTCGAC CTGAAGGCCG AGGGCGTCAC GGTGCGGCCC ATCGTCCAGT TGGGCGGCGA CACCGGGTTC GGTGAGATCT TCCTCGACGA CGTCTTCGTG CCCGACCGGG ATGTAATCGG CACCCCGCAC CAGGGCTGGC GGGCGGCGAT GAGCACGTCG AGCAACGAAC GCGGCATGTC GCTGCGCAGC CCGGCCCGCT TCCTGGCCAC CGCCGAGCGG GTGGTGCGGT TGTGGCAGGA CCGCGGGTCA CCGGCCGAGT TCGCCGACCG CGTCGCCGAC GGCTGGATCA AGGCGCAGGC CTACCGGCTG CAGACCTTCG GCACGGTCAC CCGGCTGGCC GCCGGCGGTG AGCTGGGCGC CGAATCGTCG GTGACCAAAG TGTTCTGGTC GGACCTCGAC GTCGAATTGC ACCAGACCGC CCTGGACATC CTGGCCGCCG ACGGCGAACT CGCCGGCCCG TGGACCGAGG GGCTGCTGTT CGCGCTGGGC GGCCCGATCT ACGCCGGCAC CAACGAGATT CAGCGCAACA TCATCTCCGA GCGGTTACTG GGGCTGCCGC GCGAGAAGTC CGGGAGCAAG AAG
Details for MypaA.01613.j.A1.GE29630
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 76
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDLDLDEGT LAFRDEVRQF LSANAEAIPT KSYDNAEGFE QHRRWDRVLY DAGLSVITWP KKYGGRDAPL LHWVVFEEEY FRAGAPGRAS ANGTSMLAPT LFAHGTEEQR DRILPKMASG EEIWAQAWSE PESGSDLASL RSTATPTDGG WLLNGQKIWS SRAPFAERGF GLFRSDPSAE RHHGLTYFMF DLKAEGVTVR PIVQLGGDTG FGEIFLDDVF VPDRDVIGTP HQGWRAAMST SSNERGMSLR XPARFLATAX RXXRLXQDRG SPAEXXXRVX XXWIXXQXYX AAXXXHGHPX GXXXXXGRXX XXTXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
attttgntta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggat ctggatctgg acgagggcac cctggcgttt cgggacgagg tgcggcaatt cctgtcggcc aacgccgagg cgatcccgac gaagtcgtat gacaacgccg aaggctttga gcagcaccgg cgttgggacc gggtactcta cgacgccggg ctgtcggtga tcacctggcc gaagaagtac ggcggccggg acgcgccgct gctgcactgg gtggtgttcg aagaggagta cttccgcgcc ggggcgccgg gccgggccag cgccaacggc acctcgatgc tggccccgac gctgttcgcg cacggcaccg aggagcaacg cgaccgcatc ctgccgaaaa tggctagcgg cgaagagatc tgggcgcagg cgtggtcgga gcccgagtcc ggcagcgacc tggcgtcact gcggtccacc gcgacaccga ccgacggcgg ctggctgctg aacggacaga agatctggag ctcgcgggcg ccgttcgccg agcggggctt cgggctgttc cggtccgacc ccagcgccga gcggcaccat ggcctgacgt atttcatgtt cgacctgaag gccgagggcg tcacggtgcg gcccatcgtc cagttgggcg gcgacaccgg gttcggtgag atcttcctcg acgacgtctt cgtgcccgac cgggatgtaa tcggcacccc gcaccagggc tggcgggcgg cgatgagcac gtcgagcaac gaacgcggca tgtcgctgcg cancccggcc cgcttcctgg ccaccgccga ncgggnggng cggttgnggc aggaccgcgg gtcaccggcc gagttnnccn accgcgtcnc cganngctgg atcnagnngc aggnctaccn ggctgcanan nttcngcacg gncacccnnn nggnnnnnnn nggngnnctg ggcgncnann cnnnnnnacn ngngntncnn gnnnnnnnna cnnnnantgn nnnnnacnnn ctnnnnnncn annnnnnnnn nnnnnncnnn nnnnnanngn nnctngnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntnnn nnnnnnnnnn nnnnnnnnnn nnannnnggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLDLDEGT LAFRDEVRQF LSANAEAIPT KSYDNAEGFE QHRRWDRVLY DAGLSVITWP KKYGGRDAPL LHWVVFEEEY FRAGAPGRAS ANGTSMLAPT LFAHGTEEQR DRILPKMASG EEIWAQAWSE PESGSDLASL RSTATPTDGG WLLNGQKIWS SRAPFAERGF GLFRSDPSAE RHHGLTYFMF DLKAEGVTVR PIVQLGGDTG FGEIFLDDVF VPDRDVIGTP HQGWRAAMST SSNERGMSLR SPARFLATAE RVVRLWQDRG SPAEFADRVA DGWIKAQAYR LQTFGTVTRL AAGGELGAES SVTKVFWSDL DVELHQTALD ILAADGELAG PWTEGLLFAL GGPIYAGTNE IQRNIISERL LGLPREKSGS KK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatctggat ctggacgagg gcaccctggc gtttcgggac gaggtgcggc aattcctgtc ggccaacgcc gaggcgatcc cgacgaagtc gtatgacaac gccgaaggct ttgagcagca ccggcgttgg gaccgggtac tctacgacgc cgggctgtcg gtgatcacct ggccgaagaa gtacggcggc cgggacgcgc cgctgctgca ctgggtggtg ttcgaagagg agtacttccg cgccggggcg ccgggccggg ccagcgccaa cggcacctcg atgctggccc cgacgctgtt cgcgcacggc accgaggagc aacgcgaccg catcctgccg aaaatggcta gcggcgaaga gatctgggcg caggcgtggt cggagcccga gtccggcagc gacctggcgt cactgcggtc caccgcgaca ccgaccgacg gcggctggct gctgaacgga cagaagatct ggagctcgcg ggcgccgttc gccgagcggg gcttcgggct gttccggtcc gaccccagcg ccgagcggca ccatggcctg acgtatttca tgttcgacct gaaggccgag ggcgtcacgg tgcggcccat cgtccagttg ggcggcgaca ccgggttcgg tgagatcttc ctcgacgacg tcttcgtgcc cgaccgggat gtaatcggca ccccgcacca gggctggcgg gcggcgatga gcacgtcgag caacgaacgc ggcatgtcgc tgcgcagccc ggcccgcttc ctggccaccg ccgagcgggt ggtgcggttg tggcaggacc gcgggtcacc ggccgagttc gccgaccgcg tcgccgacgg ctggatcaag gcgcaggcct accggctgca gaccttcggc acggtcaccc ggctggccgc cggcggtgag ctgggcgccg aatcgtcggt gaccaaagtg ttctggtcgg acctcgacgt cgaattgcac cagaccgccc tggacatcct ggccgccgac ggcgaactcg ccggcccgtg gaccgagggg ctgctgttcg cgctgggcgg cccgatctac gccggcacca acgagattca gcgcaacatc atctccgagc ggttactggg gctgccgcgc gagaagtccg ggagcaagaa gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa