MypaA.01613.i

FadE30

CENTER ID: MypaA.01613.i
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01613.i.A1.GE29629 Full length( MypaA.01613.i ) 1 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01613.i.A1.PS00774 Full length( MypaA.01613.i ) 1 382

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.538
RefSeq: NP_959441.1
UniProt: Q743T4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQDVEEFRAE VRSWLADNLV GEFAALKGLG GPGREHEAFE ERRAWNQHLA KAGLTCLGWP VEHGGRGLST AHRVAFYEEY ARADAPDKVN HFGEELLGPT LIAFGTPEQQ QRFLPRILDV TELWCQGYSE PGAGSDLANV STTAELDGDQ WVINGQKVWT SLAHLSQWCF VVARTEKGSK RHAGLSYLLV PLDQPGVQIR PIVQITGTAE FNEVFFDDAR TDASMVVGQP GDGWRVAMGT LTFERGVSTL GQQIRYAREL SNLAELARRN GAADDPFIRE RLTRAWTGLR AMRSYALATM DVEQPGQDNV SKLLWANWHR ALGELAMDVI GKPGMTMPDG EFDEWQRLFL FTRADTIYGG SNEIQRNIIA ERVLGLPREV KG
NT Sequence
ATGCAGGACG TCGAGGAGTT CCGGGCCGAG GTCCGCTCTT GGCTCGCCGA CAATCTGGTC GGCGAATTCG CCGCTCTGAA GGGCCTCGGC GGCCCCGGCC GCGAGCACGA GGCCTTCGAG GAACGCCGGG CCTGGAACCA GCACCTGGCC AAGGCCGGGC TGACCTGCCT GGGCTGGCCG GTCGAGCACG GCGGGCGCGG GCTGTCCACC GCGCACCGGG TGGCCTTCTA CGAGGAGTAC GCCCGCGCCG ACGCGCCGGA CAAGGTCAAC CACTTCGGCG AGGAGCTGCT CGGCCCCACG CTGATCGCGT TCGGCACCCC CGAGCAGCAG CAGCGCTTCC TGCCCCGCAT CCTCGACGTC ACCGAGCTGT GGTGCCAGGG GTATTCCGAG CCCGGCGCCG GCAGCGACCT GGCCAACGTG TCGACCACCG CCGAACTCGA CGGCGACCAG TGGGTGATCA ACGGCCAGAA GGTGTGGACC TCGCTGGCGC ACCTGTCGCA GTGGTGCTTC GTGGTCGCGC GCACCGAGAA GGGCTCCAAG CGGCACGCCG GGCTGTCGTA TCTGCTGGTG CCGCTGGACC AGCCGGGGGT GCAGATCCGG CCCATCGTGC AGATCACCGG CACCGCGGAG TTCAACGAGG TGTTCTTCGA CGACGCCCGC ACCGACGCGT CGATGGTGGT CGGCCAGCCC GGCGACGGCT GGCGGGTCGC CATGGGAACG CTGACCTTCG AGCGCGGCGT GTCCACGCTC GGGCAGCAGA TCCGTTACGC GCGTGAGCTT TCCAACCTGG CCGAGCTCGC GCGGCGCAAC GGCGCCGCCG ACGACCCGTT CATCCGGGAG CGGCTGACCC GCGCCTGGAC CGGGCTGCGC GCGATGCGGT CGTATGCCCT GGCCACCATG GACGTCGAGC AGCCCGGGCA GGACAACGTG TCGAAGTTGT TGTGGGCCAA CTGGCATCGG GCTCTCGGCG AGCTGGCCAT GGACGTCATC GGCAAGCCCG GCATGACCAT GCCCGACGGC GAATTCGACG AGTGGCAGCG GTTGTTCCTG TTCACCCGCG CCGACACCAT CTACGGCGGC TCCAACGAGA TCCAGCGCAA CATCATCGCC GAGCGGGTGC TCGGCCTGCC CCGGGAGGTA AAGGGA
Details for MypaA.01613.i.A1.GE29629
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MQDVEEFRAE VRSWLADNLV GEFAALKGLG GPGREHEAFE ERRAWNQHLA KAGLTCLGWP VEHGGRGLST AHRVAFYEEY ARADAPDKVN HFGEELLGPT LIAFGTPEQQ QRFLPRILDV TELWCQGYSE PGAGSDLANV STTAELDGDQ WVINGQKVWT SLAHLSQWCF VVARTEKGSK RHAGLSYLLV PLDQPGVQIR PIVQITGTAE FNEVFFDDAR TDASMVVGQP GDGWRVAMGT LTFERGVSTL GQQIRYAREL SNLAELARRN GAADDPFIRE RLTRAWTGLR AMRSYALATM DVEQPGQDNV SKLLWANWHR ALGELAMDVI GKPGMTMPDG EFDEWQRLFL FTRADTIYGG SNEIQRNIIA ERVLGLPREV
Validated NT Sequence
ttacctcccg gggcaggccg agcacccgct cggcgatgat gttgcgctgg atctcgttgg agccgccgta gatggtgtcg gcgcgggtga acaggaacaa ccgctgccac tcgtcgaatt cgccgtcggg catggtcatg ccgggcttgc cgatgacgtc catggccagc tcgccgagag cccgatgcca gttggcccac aacaacttcg acacgttgtc ctgcccgggc tgctcgacgt ccatggtggc cagggcatac gaccgcatcg cgcgcagccc ggtccaggcg cgggtcagcc gctcccggat gaacgggtcg tcggcggcgc cgttgcgccg cgcgagctcg gccaggttgg aaagctcacg cgcgtaacgg atctgctgcc cgagcgtgga cacgccgcgc tcgaaggtca gcgttcccat ggcgacccgc cagccgtcgc cgggctggcc gaccaccatc gacgcgtcgg tgcgggcgtc gtcgaagaac acctcgttga actccgcggt gccggtgatc tgcacgatgg gccggatctg cacccccggc tggtccagcg gcaccagcag atacgacagc ccggcgtgcc gcttggagcc cttctcggtg cgcgcgacca cgaagcacca ctgcgacagg tgcgccagcg aggtccacac cttctggccg ttgatcaccc actggtcgcc gtcgagttcg gcggtggtcg acacgttggc caggtcgctg ccggcgccgg gctcggaata cccctggcac cacagctcgg tgacgtcgag gatgcggggc aggaagcgct gctgctgctc gggggtgccg aacgcgatca gcgtggggcc gagcagctcc tcgccgaagt ggttgacctt gtccggcgcg tcggcgcggg cgtactcctc gtagaaggcc acccggtgcg cggtggacag cccgcgcccg ccgtgctcga ccggccagcc caggcaggtc agcccggcct tggccaggtg ctggttccag gcccggcgtt cctcgaaggc ctcgtgctcg cggccggggc cgccgaggcc cttcagagcg gcgaattcgc cgaccagatt gtcggcgagc caagagcgga cctcggcccg gaactcctcg acgtcctgca tcgaaccagg accctgggtc tgagcttcca gggtac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQDVEEFRA EVRSWLADNL VGEFAALKGL GGPGREHEAF EERRAWNQHL AKAGLTCLGW PVEHGGRGLS TAHRVAFYEE YARADAPDKV NHFGEELLGP TLIAFGTPEQ QQRFLPRILD VTELWCQGYS EPGAGSDLAN VSTTAELDGD QWVINGQKVW TSLAHLSQWC FVVARTEKGS KRHAGLSYLL VPLDQPGVQI RPIVQITGTA EFNEVFFDDA RTDASMVVGQ PGDGWRVAMG TLTFERGVST LGQQIRYARE LSNLAELARR NGAADDPFIR ERLTRAWTGL RAMRSYALAT MDVEQPGQDN VSKLLWANWH RALGELAMDV IGKPGMTMPD GEFDEWQRLF LFTRADTIYG GSNEIQRNII AERVLGLPRE VKG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaggacgtc gaggagttcc gggccgaggt ccgctcttgg ctcgccgaca atctggtcgg cgaattcgcc gctctgaagg gcctcggcgg ccccggccgc gagcacgagg ccttcgagga acgccgggcc tggaaccagc acctggccaa ggccgggctg acctgcctgg gctggccggt cgagcacggc gggcgcgggc tgtccaccgc gcaccgggtg gccttctacg aggagtacgc ccgcgccgac gcgccggaca aggtcaacca cttcggcgag gagctgctcg gccccacgct gatcgcgttc ggcacccccg agcagcagca gcgcttcctg ccccgcatcc tcgacgtcac cgagctgtgg tgccaggggt attccgagcc cggcgccggc agcgacctgg ccaacgtgtc gaccaccgcc gaactcgacg gcgaccagtg ggtgatcaac ggccagaagg tgtggacctc gctggcgcac ctgtcgcagt ggtgcttcgt ggtcgcgcgc accgagaagg gctccaagcg gcacgccggg ctgtcgtatc tgctggtgcc gctggaccag ccgggggtgc agatccggcc catcgtgcag atcaccggca ccgcggagtt caacgaggtg ttcttcgacg acgcccgcac cgacgcgtcg atggtggtcg gccagcccgg cgacggctgg cgggtcgcca tgggaacgct gaccttcgag cgcggcgtgt ccacgctcgg gcagcagatc cgttacgcgc gtgagctttc caacctggcc gagctcgcgc ggcgcaacgg cgccgccgac gacccgttca tccgggagcg gctgacccgc gcctggaccg ggctgcgcgc gatgcggtcg tatgccctgg ccaccatgga cgtcgagcag cccgggcagg acaacgtgtc gaagttgttg tgggccaact ggcatcgggc tctcggcgag ctggccatgg acgtcatcgg caagcccggc atgaccatgc ccgacggcga attcgacgag tggcagcggt tgttcctgtt cacccgcgcc gacaccatct acggcggctc caacgagatc cagcgcaaca tcatcgccga gcgggtgctc ggcctgcccc gggaggtaaa gggaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MypaA.01613.i.A1.PS00774
PURIFICATION DATe: 9/17/2010
CONCENTRATION: 45.08mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MQDVEEFRAE VRSWLADNLV GEFAALKGLG GPGREHEAFE ERRAWNQHLA KAGLTCLGWP VEHGGRGLST AHRVAFYEEY ARADAPDKVN HFGEELLGPT LIAFGTPEQQ QRFLPRILDV TELWCQGYSE PGAGSDLANV STTAELDGDQ WVINGQKVWT SLAHLSQWCF VVARTEKGSK RHAGLSYLLV PLDQPGVQIR PIVQITGTAE FNEVFFDDAR TDASMVVGQP GDGWRVAMGT LTFERGVSTL GQQIRYAREL SNLAELARRN GAADDPFIRE RLTRAWTGLR AMRSYALATM DVEQPGQDNV SKLLWANWHR ALGELAMDVI GKPGMTMPDG EFDEWQRLFL FTRADTIYGG SNEIQRNIIA ERVLGLPREV
Validated NT Sequence
ttacctcccg gggcaggccg agcacccgct cggcgatgat gttgcgctgg atctcgttgg agccgccgta gatggtgtcg gcgcgggtga acaggaacaa ccgctgccac tcgtcgaatt cgccgtcggg catggtcatg ccgggcttgc cgatgacgtc catggccagc tcgccgagag cccgatgcca gttggcccac aacaacttcg acacgttgtc ctgcccgggc tgctcgacgt ccatggtggc cagggcatac gaccgcatcg cgcgcagccc ggtccaggcg cgggtcagcc gctcccggat gaacgggtcg tcggcggcgc cgttgcgccg cgcgagctcg gccaggttgg aaagctcacg cgcgtaacgg atctgctgcc cgagcgtgga cacgccgcgc tcgaaggtca gcgttcccat ggcgacccgc cagccgtcgc cgggctggcc gaccaccatc gacgcgtcgg tgcgggcgtc gtcgaagaac acctcgttga actccgcggt gccggtgatc tgcacgatgg gccggatctg cacccccggc tggtccagcg gcaccagcag atacgacagc ccggcgtgcc gcttggagcc cttctcggtg cgcgcgacca cgaagcacca ctgcgacagg tgcgccagcg aggtccacac cttctggccg ttgatcaccc actggtcgcc gtcgagttcg gcggtggtcg acacgttggc caggtcgctg ccggcgccgg gctcggaata cccctggcac cacagctcgg tgacgtcgag gatgcggggc aggaagcgct gctgctgctc gggggtgccg aacgcgatca gcgtggggcc gagcagctcc tcgccgaagt ggttgacctt gtccggcgcg tcggcgcggg cgtactcctc gtagaaggcc acccggtgcg cggtggacag cccgcgcccg ccgtgctcga ccggccagcc caggcaggtc agcccggcct tggccaggtg ctggttccag gcccggcgtt cctcgaaggc ctcgtgctcg cggccggggc cgccgaggcc cttcagagcg gcgaattcgc cgaccagatt gtcggcgagc caagagcgga cctcggcccg gaactcctcg acgtcctgca tcgaaccagg accctgggtc tgagcttcca gggtac
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQDVEEFRA EVRSWLADNL VGEFAALKGL GGPGREHEAF EERRAWNQHL AKAGLTCLGW PVEHGGRGLS TAHRVAFYEE YARADAPDKV NHFGEELLGP TLIAFGTPEQ QQRFLPRILD VTELWCQGYS EPGAGSDLAN VSTTAELDGD QWVINGQKVW TSLAHLSQWC FVVARTEKGS KRHAGLSYLL VPLDQPGVQI RPIVQITGTA EFNEVFFDDA RTDASMVVGQ PGDGWRVAMG TLTFERGVST LGQQIRYARE LSNLAELARR NGAADDPFIR ERLTRAWTGL RAMRSYALAT MDVEQPGQDN VSKLLWANWH RALGELAMDV IGKPGMTMPD GEFDEWQRLF LFTRADTIYG GSNEIQRNII AERVLGLPRE VKG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAGGACGTC GAGGAGTTCC GGGCCGAGGT CCGCTCTTGG CTCGCCGACA ATCTGGTCGG CGAATTCGCC GCTCTGAAGG GCCTCGGCGG CCCCGGCCGC GAGCACGAGG CCTTCGAGGA ACGCCGGGCC TGGAACCAGC ACCTGGCCAA GGCCGGGCTG ACCTGCCTGG GCTGGCCGGT CGAGCACGGC GGGCGCGGGC TGTCCACCGC GCACCGGGTG GCCTTCTACG AGGAGTACGC CCGCGCCGAC GCGCCGGACA AGGTCAACCA CTTCGGCGAG GAGCTGCTCG GCCCCACGCT GATCGCGTTC GGCACCCCCG AGCAGCAGCA GCGCTTCCTG CCCCGCATCC TCGACGTCAC CGAGCTGTGG TGCCAGGGGT ATTCCGAGCC CGGCGCCGGC AGCGACCTGG CCAACGTGTC GACCACCGCC GAACTCGACG GCGACCAGTG GGTGATCAAC GGCCAGAAGG TGTGGACCTC GCTGGCGCAC CTGTCGCAGT GGTGCTTCGT GGTCGCGCGC ACCGAGAAGG GCTCCAAGCG GCACGCCGGG CTGTCGTATC TGCTGGTGCC GCTGGACCAG CCGGGGGTGC AGATCCGGCC CATCGTGCAG ATCACCGGCA CCGCGGAGTT CAACGAGGTG TTCTTCGACG ACGCCCGCAC CGACGCGTCG ATGGTGGTCG GCCAGCCCGG CGACGGCTGG CGGGTCGCCA TGGGAACGCT GACCTTCGAG CGCGGCGTGT CCACGCTCGG GCAGCAGATC CGTTACGCGC GTGAGCTTTC CAACCTGGCC GAGCTCGCGC GGCGCAACGG CGCCGCCGAC GACCCGTTCA TCCGGGAGCG GCTGACCCGC GCCTGGACCG GGCTGCGCGC GATGCGGTCG TATGCCCTGG CCACCATGGA CGTCGAGCAG CCCGGGCAGG ACAACGTGTC GAAGTTGTTG TGGGCCAACT GGCATCGGGC TCTCGGCGAG CTGGCCATGG ACGTCATCGG CAAGCCCGGC ATGACCATGC CCGACGGCGA ATTCGACGAG TGGCAGCGGT TGTTCCTGTT CACCCGCGCC GACACCATCT ACGGCGGCTC CAACGAGATC CAGCGCAACA TCATCGCCGA GCGGGTGCTC GGCCTGCCCC GGGAGGTAAA GGGAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA