MypaA.01613.g

Putative uncharacterized protein

CENTER ID: MypaA.01613.g
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01613.g.A1.GE29627 Full length( MypaA.01613.g ) 1 359
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.755
RefSeq: NP_959650.1
UniProt: Q742W9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARRQRSDPR ARPFPAGPHE AVRPGQPRSL RRRLDAVRVA GRSRWPAEVG GLGGPAVLRA VVGEEVVGRR LAEPGPYSML EVLAPTMIDY APAELAAEMV PRLLRGEEHW CQGFSEPGSG SDLASLTTRA TQDGDHWIVN GQKVWTSFAQ FSTRCVLLTR TAPGYDGITA FFVDLDTPGI TIRPLRTMHG VDEFCEVYYD DVAIPSSRML GRPGDGWRLA MDLLPYERSS CFWQRIAYLY SRFDDLIAEV SAAGEPDESS LGAAYLALHT LRCRSRATQH RMLDGARLGP DTSIDKVLLA TAEQRLYDTV RDLLPGTLEL ENTPWRSEYL YSRAATIYGG TAEIQRNIIA RRLLDLGKE
NT Sequence
GTGGCTCGAC GACAACGATC TGACCCCCGG GCCCGACCAT TCCCTGCAGG GCCACATGAA GCAGTTCGCC CGGGTCAGCC GCGCTCTCTA CGACGCCGAC TGGATGCGGT ACGGGTGGCC GGCCGAAGTC GGTGGCCGGC CGAAGTCGGT GGCCTGGGCG GACCCGCGGT GCTGCGGGCG GTCGTCGGCG AGGAAGTGGT GGGCCGCCGG CTGGCCGAAC CCGGACCCTA CTCGATGCTC GAGGTGCTGG CCCCCACCAT GATCGACTAC GCCCCCGCCG AACTGGCCGC GGAGATGGTG CCGCGGCTGC TGCGCGGCGA AGAGCACTGG TGTCAAGGCT TTTCCGAGCC GGGGTCGGGC AGCGACCTGG CGTCGCTGAC TACCCGCGCG ACCCAGGACG GCGACCACTG GATCGTCAAC GGGCAGAAGG TCTGGACCAG CTTCGCGCAG TTCTCCACCC GGTGCGTGCT GCTGACCCGC ACCGCGCCCG GCTACGACGG GATCACCGCC TTCTTCGTCG ACCTGGACAC GCCGGGTATC ACCATCCGGC CGCTGCGCAC CATGCACGGC GTCGACGAAT TCTGCGAGGT GTACTACGAC GACGTGGCGA TTCCGTCGAG CCGCATGCTC GGCCGGCCCG GCGACGGCTG GCGCCTGGCC ATGGATCTGC TGCCGTATGA ACGGTCCTCC TGCTTCTGGC AGCGCATCGC CTACCTCTAC TCGCGTTTCG ACGATCTGAT CGCCGAGGTC TCGGCGGCCG GGGAGCCCGA CGAATCCTCC CTGGGCGCAG CCTATTTGGC GCTGCACACG TTGCGCTGCC GGTCGCGCGC CACCCAGCAC CGGATGCTTG ACGGGGCCCG GCTGGGGCCC GACACCTCGA TCGACAAGGT GCTGCTGGCC ACCGCCGAGC AGCGGCTCTA CGACACCGTG CGCGACCTGT TGCCGGGCAC CCTGGAGCTC GAAAACACGC CGTGGCGCTC GGAATACCTG TACTCGCGCG CGGCCACCAT CTACGGCGGT ACCGCCGAGA TCCAGCGCAA CATCATCGCC CGCCGGCTGC TCGATCTCGG GAAGGAG
Details for MypaA.01613.g.A1.GE29627
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 77
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVARRQRSD PRARPFPAGP HEAVRPGQPR SLRRRLDAVR VAGRSRWPAE VGGLGGPAVL RAVVGEEVVG RRLAEPGPYS MLEVLAPTMI DYAPAELAAE MVPRLLRGEE HWCQGFSEPG SGSDLASLTT RATQDGDHWI VNGQKVWTSF AQFSTRCVLL TRTAPGYDGI TAFFVDLDTP GITIRPLRTM HGVDEFCEVY YDDVAIPSSR MLGRPGDGWR LXXXLLPYER SSCFWQRIAY LYSRFDDLIA XVSAAGEPDX SSLGAXXLXL XXXXXRSRXP XXXXXGXXWG PXXSIXXXCX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
ttgtttanct ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtggct cgacgacaac gatctgaccc ccgggcccga ccattccctg cagggccaca tgaagcagtt cgcccgggtc agccgcgctc tctacgacgc cgactggatg cggtacgggt ggccggccga agtcggtggc cggccgaagt cggtggcctg ggcggacccg cggtgctgcg ggcggtcgtc ggcgaggaag tggtgggccg ccggctggcc gaacccggac cctactcgat gctcgaggtg ctggccccca ccatgatcga ctacgccccc gccgaactgg ccgcggagat ggtgccgcgg ctgctgcgcg gcgaagagca ctggtgtcaa ggcttttccg agccggggtc gggcagcgac ctggcgtcgc tgactacccg cgcgacccag gacggcgacc actggatcgt caacgggcag aaggtctgga ccagcttcgc gcagttctcc acccggtgcg tgctgctgac ccgcaccgcg cccggctacg acgggatcac cgccttcttc gtcgacctgg acacgccggg tatcaccatc cggccgctgc gcaccatgca cggcgtcgac gaattctgcg aggtgtacta cgacgacgtg gcgattccgt cgagccgcat gctcggccgg cccggcgacg gctggcgcct ggncatngnt ctgctgccgt atgaacggtc ctcctgcttc tggcagcgca tcgcctacct ctactcgcgt ttcgacgatc tgatcgccga ngtctcggcg gccggggagc ccgacnaatc ntccctgggc gcanccnatt tggnnctgna cncgnngnnc tnccggtcgc gnnnccccan nncnnatgnt nnnggnncnn gctggggncc gnnnncntcg atcganangn gctgcnngcn ncnnnnanca nnnnnnnnnc nannnnnnnn nnacnntgnn nnnncnnnnn ntngaaannn nncnngcnnn nnnnnnnnnn nacngnngnn cnnnnnnnnn annnnnnntn gnnnnnnnnn nnnnnnnnna nnnnnnnnnn nnngnnnnnn nnnnnnnngn nnnnana
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVARRQRSD PRARPFPAGP HEAVRPGQPR SLRRRLDAVR VAGRSRWPAE VGGLGGPAVL RAVVGEEVVG RRLAEPGPYS MLEVLAPTMI DYAPAELAAE MVPRLLRGEE HWCQGFSEPG SGSDLASLTT RATQDGDHWI VNGQKVWTSF AQFSTRCVLL TRTAPGYDGI TAFFVDLDTP GITIRPLRTM HGVDEFCEVY YDDVAIPSSR MLGRPGDGWR LAMDLLPYER SSCFWQRIAY LYSRFDDLIA EVSAAGEPDE SSLGAAYLAL HTLRCRSRAT QHRMLDGARL GPDTSIDKVL LATAEQRLYD TVRDLLPGTL ELENTPWRSE YLYSRAATIY GGTAEIQRNI IARRLLDLGK E
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggctcga cgacaacgat ctgacccccg ggcccgacca ttccctgcag ggccacatga agcagttcgc ccgggtcagc cgcgctctct acgacgccga ctggatgcgg tacgggtggc cggccgaagt cggtggccgg ccgaagtcgg tggcctgggc ggacccgcgg tgctgcgggc ggtcgtcggc gaggaagtgg tgggccgccg gctggccgaa cccggaccct actcgatgct cgaggtgctg gcccccacca tgatcgacta cgcccccgcc gaactggccg cggagatggt gccgcggctg ctgcgcggcg aagagcactg gtgtcaaggc ttttccgagc cggggtcggg cagcgacctg gcgtcgctga ctacccgcgc gacccaggac ggcgaccact ggatcgtcaa cgggcagaag gtctggacca gcttcgcgca gttctccacc cggtgcgtgc tgctgacccg caccgcgccc ggctacgacg ggatcaccgc cttcttcgtc gacctggaca cgccgggtat caccatccgg ccgctgcgca ccatgcacgg cgtcgacgaa ttctgcgagg tgtactacga cgacgtggcg attccgtcga gccgcatgct cggccggccc ggcgacggct ggcgcctggc catggatctg ctgccgtatg aacggtcctc ctgcttctgg cagcgcatcg cctacctcta ctcgcgtttc gacgatctga tcgccgaggt ctcggcggcc ggggagcccg acgaatcctc cctgggcgca gcctatttgg cgctgcacac gttgcgctgc cggtcgcgcg ccacccagca ccggatgctt gacggggccc ggctggggcc cgacacctcg atcgacaagg tgctgctggc caccgccgag cagcggctct acgacaccgt gcgcgacctg ttgccgggca ccctggagct cgaaaacacg ccgtggcgct cggaatacct gtactcgcgc gcggccacca tctacggcgg taccgccgag atccagcgca acatcatcgc ccgccggctg ctcgatctcg ggaaggagaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa