MypaA.01613.d

FadE17

CENTER ID: MypaA.01613.d
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01613.d.A1.GE29624 Full length( MypaA.01613.d ) 1 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.2370
RefSeq: NP_961161.1
UniProt: Q73XT2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQLALTPEEA AFRDELRTIY TTKIPEELRE RVRRGSAEVN RDDIVTSHKI LHEHGLAVPN WPVEWGGKDW TPTQHQIWAD EMQLACVPEP LNFNTKMVGP VIAEFGSEEV KQRFLPPTAS LDIWWCQGFS EPDAGSDLAS LRTTAVRDGD HYIVNGQKTW TTLGQYADWI FCLVRTDPQA PKRQAGISFL LFDMKSPGIT LRPIKTIDGG HEINEVFFED VRVPADQLVG EENQGWTYAK FLLGNERTGI AGVGRTKVRL AEVKKHAAAT GVLDDPLFAA RLAEAENELL ALELTQSRVT TDSANGQPNP ASSVLKLRGS QLQQVATELL VEVAGPDALP AKADDIASPS WAQSSAPHYL NYRKTSIYGG SSEVQRNIIA STILGL
NT Sequence
ATGCAACTGG CGCTCACACC GGAGGAAGCC GCGTTCCGCG ACGAGCTTCG CACCATCTAC ACGACCAAGA TTCCCGAGGA GCTGCGCGAG CGGGTGCGCC GGGGATCGGC GGAAGTGAAC CGCGACGACA TCGTCACCAG CCACAAGATC CTGCACGAGC ACGGCCTTGC GGTACCGAAC TGGCCGGTGG AGTGGGGCGG CAAGGACTGG ACGCCCACCC AGCACCAGAT CTGGGCCGAC GAGATGCAGC TGGCCTGCGT CCCGGAGCCG TTGAACTTCA ACACCAAGAT GGTGGGCCCG GTGATCGCCG AATTCGGTTC GGAGGAGGTC AAACAACGCT TCCTGCCCCC GACCGCCAGC CTCGACATCT GGTGGTGCCA GGGATTCTCC GAGCCGGACG CCGGTTCGGA CCTGGCGTCG CTGCGCACCA CCGCGGTGCG CGACGGCGAC CACTACATCG TCAACGGCCA GAAGACCTGG ACCACGCTGG GCCAGTACGC CGACTGGATC TTCTGCCTGG TGCGCACCGA CCCGCAGGCG CCCAAGCGCC AGGCCGGCAT CTCGTTCCTG CTCTTCGACA TGAAAAGCCC GGGCATCACG CTGCGGCCCA TCAAGACGAT CGACGGCGGC CACGAGATCA ACGAGGTCTT CTTCGAGGAC GTCCGGGTGC CCGCCGATCA GCTTGTCGGA GAAGAGAATC AGGGCTGGAC GTACGCGAAG TTCCTGCTGG GCAACGAACG CACCGGCATC GCCGGAGTGG GCCGCACCAA GGTGCGCCTG GCCGAGGTGA AGAAGCACGC CGCGGCCACC GGGGTGCTCG ACGATCCGCT GTTCGCCGCC CGGCTCGCCG AGGCCGAAAA CGAGCTGCTG GCACTGGAAC TCACGCAGTC GCGGGTGACC ACCGACTCGG CGAACGGGCA GCCCAACCCG GCGTCGTCGG TGCTCAAGCT GCGGGGCAGC CAACTGCAGC AGGTGGCCAC CGAACTGCTC GTCGAGGTGG CCGGCCCGGA TGCGTTGCCG GCCAAGGCCG ACGACATCGC CTCCCCGTCC TGGGCGCAAT CCAGCGCGCC GCACTACCTG AACTATCGCA AGACGTCGAT CTACGGCGGC AGCAGCGAAG TACAGCGCAA CATCATCGCG TCCACCATTC TGGGATTG
Details for MypaA.01613.d.A1.GE29624
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQLALTPEE AAFRDELRTI YTTKIPEELR ERVRRGSAEV NRDDIVTSHK ILHEHGLAVP NWPVEWGGKD WTPTQHQIWA DEMQLACVPE PLNFNTKMVG PVIAEFGSEE VKQRFLPPTA SLDIWWCQGF SEPDAGSDLA SLRTTAVRDG DHYIVNGQKT WTTLGQYADW IFCLVRTDPQ APKRQAGISF LLFDMKSPGI TLRPIKTIDG GHEINEVFFE DVRVPADQLV GXENQGWTYA KFLLGNERTG IAGXGRTKVR LXXVKKHAAX XGXLDDPLFA ARXAXXENEL LALXLTQSRX TTDSAXGXPX XRRRXSSXXX XXQXXXXXXX XXXXXXXXXX XXPXXXXXXX XXXXXXXXXG GXXXXXXXXX XXXX
Validated NT Sequence
ttgtttactt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgcaactgg cgctcacacc ggaggaagcc gcgttccgcg acgagcttcg caccatctac acgaccaaga ttcccgagga gctgcgcgag cgggtgcgcc ggggatcggc ggaagtgaac cgcgacgaca tcgtcaccag ccacaagatc ctgcacgagc acggccttgc ggtaccgaac tggccggtgg agtggggcgg caaggactgg acgcccaccc agcaccagat ctgggccgac gagatgcagc tggcctgcgt cccggagccg ttgaacttca acaccaagat ggtgggcccg gtgatcgccg aattcggttc ggaggaggtc aaacaacgct tcctgccccc gaccgccagc ctcgacatct ggtggtgcca gggattctcc gagccggacg ccggttcgga cctggcgtcg ctgcgcacca ccgcggtgcg cgacggcgac cactacatcg tcaacggcca gaagacctgg accacgctgg gccagtacgc cgactggatc ttctgcctgg tgcgcaccga cccgcaggcg cccaagcgcc aggccggcat ctcgttcctg ctcttcgaca tgaaaagccc gggcatcacg ctgcggccca tcaagacgat cgacggcggc cacgagatca acgaggtctt cttcgaggac gtccgggtgc ccgccgatca gcttgtcgga naagagaatc agggctggac gtacgcgaag ttcctgctgg gcaacgaacg caccggcatc gccggantgg gccgcaccaa ggtgcgcctg gncgangtga agaagcacgc cgcnnnnncc ggggngctcg acgatccgct gttcgccgcc cggntcgccg angncgaaaa cgagctgctg gcactgnnnc tcacgcagtc gcgggngacn accgactcgg cganngggnn nccnnnnnnn cgtcgtcggn gntcnagctn nngggnannn nngcagcnnn nnnnnnnnnn nnncnncnnn nnnnncnnng nnnnnnnnnn nnannannnn nncccgncnn ngnnnannan nnnnnnnnnn nnnannnnnn nnnnnnnnnn nnnnggnggn agnnnnnnnn nnnnnnnnnt nnnnnnncnn cnnnnnnnnn g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQLALTPEE AAFRDELRTI YTTKIPEELR ERVRRGSAEV NRDDIVTSHK ILHEHGLAVP NWPVEWGGKD WTPTQHQIWA DEMQLACVPE PLNFNTKMVG PVIAEFGSEE VKQRFLPPTA SLDIWWCQGF SEPDAGSDLA SLRTTAVRDG DHYIVNGQKT WTTLGQYADW IFCLVRTDPQ APKRQAGISF LLFDMKSPGI TLRPIKTIDG GHEINEVFFE DVRVPADQLV GEENQGWTYA KFLLGNERTG IAGVGRTKVR LAEVKKHAAA TGVLDDPLFA ARLAEAENEL LALELTQSRV TTDSANGQPN PASSVLKLRG SQLQQVATEL LVEVAGPDAL PAKADDIASP SWAQSSAPHY LNYRKTSIYG GSSEVQRNII ASTILGL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaactggcg ctcacaccgg aggaagccgc gttccgcgac gagcttcgca ccatctacac gaccaagatt cccgaggagc tgcgcgagcg ggtgcgccgg ggatcggcgg aagtgaaccg cgacgacatc gtcaccagcc acaagatcct gcacgagcac ggccttgcgg taccgaactg gccggtggag tggggcggca aggactggac gcccacccag caccagatct gggccgacga gatgcagctg gcctgcgtcc cggagccgtt gaacttcaac accaagatgg tgggcccggt gatcgccgaa ttcggttcgg aggaggtcaa acaacgcttc ctgcccccga ccgccagcct cgacatctgg tggtgccagg gattctccga gccggacgcc ggttcggacc tggcgtcgct gcgcaccacc gcggtgcgcg acggcgacca ctacatcgtc aacggccaga agacctggac cacgctgggc cagtacgccg actggatctt ctgcctggtg cgcaccgacc cgcaggcgcc caagcgccag gccggcatct cgttcctgct cttcgacatg aaaagcccgg gcatcacgct gcggcccatc aagacgatcg acggcggcca cgagatcaac gaggtcttct tcgaggacgt ccgggtgccc gccgatcagc ttgtcggaga agagaatcag ggctggacgt acgcgaagtt cctgctgggc aacgaacgca ccggcatcgc cggagtgggc cgcaccaagg tgcgcctggc cgaggtgaag aagcacgccg cggccaccgg ggtgctcgac gatccgctgt tcgccgcccg gctcgccgag gccgaaaacg agctgctggc actggaactc acgcagtcgc gggtgaccac cgactcggcg aacgggcagc ccaacccggc gtcgtcggtg ctcaagctgc ggggcagcca actgcagcag gtggccaccg aactgctcgt cgaggtggcc ggcccggatg cgttgccggc caaggccgac gacatcgcct ccccgtcctg ggcgcaatcc agcgcgccgc actacctgaa ctatcgcaag acgtcgatct acggcggcag cagcgaagta cagcgcaaca tcatcgcgtc caccattctg ggattgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa