MypaA.01613.a

Putative uncharacterized protein

CENTER ID: MypaA.01613.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01613.a.A1.GE29621 Full length( MypaA.01613.a ) 1 391
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.3816
RefSeq: NP_962519.1
UniProt: Q73TY3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFSRVELSA QDAAFQQSTR AFLAEHVTEE VRRRDRETGE NFSEPVHVAL GEAGYLESDW KLESEGGFDP VRRRIFQLEI GRAHTPWFHW GTTAVVARLV KQFGAPELAD AILPGVLSGK IRLCLGYTEP EGGSDVATCK TRAVRDGSGW IINGSKMFTS NAQNAEYVFL LTNTDPQRPK HKNLTMFLVP LDSPGIEIQG IRTVDGDRTN IVYYSDVRVD DLYRIGEVNG GWTVMRFALD AEHGLTDPQD HGLQNISMLS EHGHLMAEAA DRVAAILARP DAAGRRPIDD ESVKYRLGRG MARIEAAMST PGMYGRVALI QTMREVSQEL MDLLGTASAL PTDTPGSPDD GAIEFIFRHA VPAGIYGGTM EVFRNMIAQH ELGLGRPSYG R
NT Sequence
ATGGACTTCT CCCGGGTAGA ACTTTCGGCG CAGGACGCGG CCTTCCAGCA GAGCACGCGC GCGTTCCTGG CCGAGCACGT CACCGAAGAG GTGCGCCGGC GTGATCGCGA AACCGGCGAA AACTTCAGCG AGCCGGTGCA TGTGGCGCTG GGTGAAGCGG GTTACCTGGA ATCGGACTGG AAGCTGGAGT CCGAGGGCGG ATTCGATCCG GTACGCCGGC GGATTTTTCA GCTCGAGATC GGCCGCGCGC ACACGCCGTG GTTCCACTGG GGCACCACGG CGGTGGTCGC GCGGTTGGTC AAGCAATTCG GGGCGCCCGA ACTCGCCGAC GCGATCCTGC CCGGCGTGCT GTCCGGGAAG ATCCGGCTCT GCCTGGGCTA CACCGAACCC GAGGGCGGCT CCGACGTCGC CACCTGCAAA ACCCGGGCCG TGCGGGACGG GTCGGGCTGG ATCATCAACG GCTCCAAGAT GTTCACCTCG AACGCGCAGA ACGCCGAGTA CGTCTTCCTG CTCACCAACA CCGATCCGCA GCGGCCGAAA CACAAGAACC TGACCATGTT CCTGGTGCCG CTCGACTCAC CGGGCATCGA GATCCAGGGC ATCCGCACCG TCGACGGCGA CCGCACCAAC ATCGTCTACT ACAGTGACGT ACGCGTCGAC GACCTCTACC GCATCGGCGA GGTCAACGGC GGCTGGACGG TGATGCGGTT CGCCCTCGAC GCCGAACACG GCCTCACCGA CCCCCAAGAC CATGGCCTGC AGAACATTTC GATGCTTTCC GAGCACGGCC ACCTGATGGC CGAGGCGGCC GACAGGGTCG CGGCCATCCT GGCCAGACCC GATGCGGCCG GCCGCCGGCC GATCGACGAC GAGTCGGTCA AATACCGGCT CGGGCGCGGC ATGGCCCGCA TCGAGGCGGC CATGTCGACC CCCGGCATGT ACGGACGCGT GGCGCTCATT CAAACCATGC GTGAGGTATC CCAGGAACTG ATGGACCTGC TGGGGACCGC ATCGGCCTTG CCCACCGACA CCCCGGGTTC ACCCGACGAC GGCGCCATCG AGTTCATCTT CCGGCACGCC GTGCCCGCCG GCATCTACGG CGGCACCATG GAGGTGTTCC GCAACATGAT CGCCCAGCAC GAGCTGGGGC TCGGACGGCC TAGCTACGGG CGC
Details for MypaA.01613.a.A1.GE29621
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 94
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDFSRVELS AQDAAFQQST RAFLAEHVTE EVRRRDRETG ENFSEPVHVA LGEAGYLESD WKLESEGGFD PVRRRIFQLE IGRAHTPWFH WGTTAVVARL VKQFGAPELA DAILPGVLSG KIRLCLGYTE PEGGSDVATC KTRAVRDGSG WIINGSKMFT SNAQNAEYVF LLTNTDPQRP KHKNLTMFLV PLDSPGIEIQ GIRTVDGDRT NIVYYSDVRV DDLYRIGEVN GGWTVMRFAL DAEHGLTDPQ XHGLQNISML SXHXHLMXEA AXRXAXILXR PXAXXXXADR RXXGQIRLGR XMXRIXAXMS XPXXXXXXXX XXXXXXXXXD XXLXRIXXXX XXXXXXGXXX XXXXXXXXXX XXXXXXXXX
Validated NT Sequence
attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggac ttctcccggg tagaactttc ggcgcaggac gcggccttcc agcagagcac gcgcgcgttc ctggccgagc acgtcaccga agaggtgcgc cggcgtgatc gcgaaaccgg cgaaaacttc agcgagccgg tgcatgtggc gctgggtgaa gcgggttacc tggaatcgga ctggaagctg gagtccgagg gcggattcga tccggtacgc cggcggattt ttcagctcga gatcggccgc gcgcacacgc cgtggttcca ctggggcacc acggcggtgg tcgcgcggtt ggtcaagcaa ttcggggcgc ccgaactcgc cgacgcgatc ctgcccggcg tgctgtccgg gaagatccgg ctctgcctgg gctacaccga acccgagggc ggctccgacg tcgccacctg caaaacccgg gccgtgcggg acgggtcggg ctggatcatc aacggctcca agatgttcac ctcgaacgcg cagaacgccg agtacgtctt cctgctcacc aacaccgatc cgcagcggcc gaaacacaag aacctgacca tgttcctggt gccgctcgac tcaccgggca tcgagatcca gggcatccgc accgtcgacg gcgaccgcac caacatcgtc tactacagtg acgtacgcgt cgacgacctc taccgcatcg gcgaggtcaa cggcggctgg acggtgatgc ggttcgccct cgacgccgaa cacggcctca ccgaccccca agancatggc ctgcagaaca tttcgatgct ttccnagcac gnccacctga tggncgaggc ggccganagg gncgcggnca tcctggncag acccnatgcg gncngncnnn cngccgatcg acgannantc ggtcaaatac gnctcgggcg nngcatggnc cgcatcnnng cngnnatgtc nanccccngn annnnngnnn nnnnnnnnnt cntnnannnt gnnnnagnnn ccannnnnga tgnacngctg gnncgnatcn gnnncnnnnc nnnnnncngg nnnnccnnng gnnnnntnnn nnnnnntnnn nnnnnnnnnn nnnnnnnnnn nnnnncnnnn nggntnnnnn nnnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDFSRVELS AQDAAFQQST RAFLAEHVTE EVRRRDRETG ENFSEPVHVA LGEAGYLESD WKLESEGGFD PVRRRIFQLE IGRAHTPWFH WGTTAVVARL VKQFGAPELA DAILPGVLSG KIRLCLGYTE PEGGSDVATC KTRAVRDGSG WIINGSKMFT SNAQNAEYVF LLTNTDPQRP KHKNLTMFLV PLDSPGIEIQ GIRTVDGDRT NIVYYSDVRV DDLYRIGEVN GGWTVMRFAL DAEHGLTDPQ DHGLQNISML SEHGHLMAEA ADRVAAILAR PDAAGRRPID DESVKYRLGR GMARIEAAMS TPGMYGRVAL IQTMREVSQE LMDLLGTASA LPTDTPGSPD DGAIEFIFRH AVPAGIYGGT MEVFRNMIAQ HELGLGRPSY GR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacttctcc cgggtagaac tttcggcgca ggacgcggcc ttccagcaga gcacgcgcgc gttcctggcc gagcacgtca ccgaagaggt gcgccggcgt gatcgcgaaa ccggcgaaaa cttcagcgag ccggtgcatg tggcgctggg tgaagcgggt tacctggaat cggactggaa gctggagtcc gagggcggat tcgatccggt acgccggcgg atttttcagc tcgagatcgg ccgcgcgcac acgccgtggt tccactgggg caccacggcg gtggtcgcgc ggttggtcaa gcaattcggg gcgcccgaac tcgccgacgc gatcctgccc ggcgtgctgt ccgggaagat ccggctctgc ctgggctaca ccgaacccga gggcggctcc gacgtcgcca cctgcaaaac ccgggccgtg cgggacgggt cgggctggat catcaacggc tccaagatgt tcacctcgaa cgcgcagaac gccgagtacg tcttcctgct caccaacacc gatccgcagc ggccgaaaca caagaacctg accatgttcc tggtgccgct cgactcaccg ggcatcgaga tccagggcat ccgcaccgtc gacggcgacc gcaccaacat cgtctactac agtgacgtac gcgtcgacga cctctaccgc atcggcgagg tcaacggcgg ctggacggtg atgcggttcg ccctcgacgc cgaacacggc ctcaccgacc cccaagacca tggcctgcag aacatttcga tgctttccga gcacggccac ctgatggccg aggcggccga cagggtcgcg gccatcctgg ccagacccga tgcggccggc cgccggccga tcgacgacga gtcggtcaaa taccggctcg ggcgcggcat ggcccgcatc gaggcggcca tgtcgacccc cggcatgtac ggacgcgtgg cgctcattca aaccatgcgt gaggtatccc aggaactgat ggacctgctg gggaccgcat cggccttgcc caccgacacc ccgggttcac ccgacgacgg cgccatcgag ttcatcttcc ggcacgccgt gcccgccggc atctacggcg gcaccatgga ggtgttccgc aacatgatcg cccagcacga gctggggctc ggacggccta gctacgggcg caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa