MypaA.01582.b

Putative uncharacterized protein

CENTER ID: MypaA.01582.b
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01582.b.A1.GE29618 Full length( MypaA.01582.b ) 1 301
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.549
RefSeq: NP_959452.1
UniProt: Q743S3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGVVDGRVVI VTGAGGGIGR AHALAFAAEG ARVVVNDIGV GLDGSPASGG SAAQSVVDEI TAAGGEAVAD GSNVADWDQA AGLIQTAVET FGGLDVLVNN AGIVRDRMIA NTSEEEFDAV IAVHLKGRFA TMRHAASYWR GLSKAGEAVD GRIINTSSGA GLQGSVGQGN YSAAKAGIAT LTLVGAAEMG RYGVTVNAIA PSARTRMTET VFAEMMATQD QDFDAMAPEN VSPLVVWLGS AEARDVTGKV FEVEGGKIRV AEGWAHGPQI DKGARWDPAE LGPVVADLLA KARPPVPVYG A
NT Sequence
ATGGGAGTGG TTGACGGCCG CGTCGTCATC GTCACCGGAG CGGGCGGCGG TATCGGTCGC GCGCACGCAC TGGCCTTCGC CGCCGAAGGG GCGCGTGTGG TGGTCAACGA CATCGGGGTG GGCCTGGACG GTTCACCGGC CAGCGGCGGC AGCGCCGCCC AGAGCGTGGT GGACGAGATC ACCGCCGCCG GCGGGGAAGC CGTCGCCGAC GGATCCAACG TCGCCGACTG GGACCAGGCC GCCGGTCTGA TCCAGACCGC CGTCGAAACC TTCGGTGGTC TCGACGTTTT GGTGAACAAC GCCGGAATCG TGCGCGACAG GATGATCGCC AACACCAGCG AGGAGGAGTT CGACGCCGTC ATCGCCGTGC ACCTCAAGGG GCGCTTCGCC ACCATGCGGC ACGCCGCGTC GTACTGGCGG GGGCTGTCCA AGGCGGGCGA GGCCGTCGAC GGCCGGATCA TCAACACCAG CTCGGGCGCC GGCCTGCAGG GCAGCGTCGG GCAGGGCAAC TACAGCGCCG CCAAGGCCGG TATCGCGACG CTGACGCTGG TCGGCGCGGC GGAGATGGGC CGTTACGGCG TCACCGTCAA CGCGATCGCG CCGTCGGCCC GCACCCGGAT GACCGAGACC GTGTTCGCCG AGATGATGGC AACCCAGGAC CAGGATTTCG ACGCGATGGC CCCGGAAAAC GTGTCGCCCC TGGTGGTTTG GCTGGGCAGT GCGGAGGCCC GCGACGTGAC CGGCAAGGTG TTCGAGGTCG AGGGCGGCAA GATCCGGGTC GCCGAGGGTT GGGCGCACGG CCCGCAGATC GACAAGGGCG CGAGGTGGGA TCCCGCCGAG CTGGGGCCCG TCGTCGCCGA CCTGCTCGCC AAGGCCCGCC CGCCGGTCCC GGTCTACGGC GCC
Details for MypaA.01582.b.A1.GE29618
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 32kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 89
PERCENT COVERAGE: 111
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGVVDGRVV IVTGAGGGIG RAHALAFAAE GARVVVNDIG VGLDGSPASG GSAAQSVVDE ITAAGGEAVA DGSNVADWDQ AAGLIQTAVE TFGGLDVLVN NAGIVRDRMI ANTSEEEFDA VIAVHLKGRF ATMRHAASYW RGLSKAGEAV DGRIINTSSG AGLQGSVGQG NYSAAKAGIA TLTLVGAAEM GRYGVTVNAI APSARTRMTE TVFAEMMATQ DQDFDAMAPE NVSPLVVWLG SAEARDVTGK VFXVXGGKIR VAXGWAHXXA XRXGREVGSR XXGXRRRXXX RXXXPXXXXX XXLINSXXSS AAXXXXXXCX XXXXXXXXXX XXXXXXXXX
Validated NT Sequence
tnnnnnnnnn nnntttgtnn nnntttaaga aggagatata ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatggg agtggttgac ggccgcgtcg tcatcgtcac cggagcgggc ggcggtatcg gtcgcgcgca cgcactggcc ttcgccgccg aaggggcgcg tgtggtggtc aacgacatcg gggtgggcct ggacggttca ccggccagcg gcggcagcgc cgcccagagc gtggtggacg agatcaccgc cgccggcggg gaagccgtcg ccgacggatc caacgtcgcc gactgggacc aggccgccgg tctgatccag accgccgtcg aaaccttcgg tggtctcgac gttttggtga acaacgccgg aatcgtgcgc gacaggatga tcgccaacac cagcgaggag gagttcgacg ccgtcatcgc cgtgcacctc aaggggcgct tcgccaccat gcggcacgcc gcgtcgtact ggcgggggct gtccaaggcg ggcgaggccg tcgacggccg gatcatcaac accagctcgg gcgccggcct gcagggcagc gtcgggcagg gcaactacag cgccgccaag gccggtatcg cgacgctgac gctggtcggc gcggcggaga tgggccgtta cggcgtcacc gtcaacgcga tcgcgccgtc ggcccgcacc cggatgaccg agaccgtgtt cgccgagatg atggcaaccc aggaccagga tttcgacgcg atggccccgg aaaacgtgtc gcccctggtg gtttggctgg gcagtgcgga ggcccgcgac gtgaccggca aggtgttcga ngtcganggc ggcaagatcc gggtcgccna gggttgggcg cacngnnccg canatcgaca ngggcgcgag gtgggntccc gcnnanctgg ggnccgtcgt cgccnanctg ntcgncnagn nngnccgnnn gnccnggnnn tanggnnnct aataaacagc nngaanagtt ctgcagcnan ctnnncnnnn ntcnnntgct annannccnn nnnnnctnnn tngnngnnnc ncnctnnnnn nnnnnnnnnn tnnnnnnaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGVVDGRVV IVTGAGGGIG RAHALAFAAE GARVVVNDIG VGLDGSPASG GSAAQSVVDE ITAAGGEAVA DGSNVADWDQ AAGLIQTAVE TFGGLDVLVN NAGIVRDRMI ANTSEEEFDA VIAVHLKGRF ATMRHAASYW RGLSKAGEAV DGRIINTSSG AGLQGSVGQG NYSAAKAGIA TLTLVGAAEM GRYGVTVNAI APSARTRMTE TVFAEMMATQ DQDFDAMAPE NVSPLVVWLG SAEARDVTGK VFEVEGGKIR VAEGWAHGPQ IDKGARWDPA ELGPVVADLL AKARPPVPVY GA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggagtggtt gacggccgcg tcgtcatcgt caccggagcg ggcggcggta tcggtcgcgc gcacgcactg gccttcgccg ccgaaggggc gcgtgtggtg gtcaacgaca tcggggtggg cctggacggt tcaccggcca gcggcggcag cgccgcccag agcgtggtgg acgagatcac cgccgccggc ggggaagccg tcgccgacgg atccaacgtc gccgactggg accaggccgc cggtctgatc cagaccgccg tcgaaacctt cggtggtctc gacgttttgg tgaacaacgc cggaatcgtg cgcgacagga tgatcgccaa caccagcgag gaggagttcg acgccgtcat cgccgtgcac ctcaaggggc gcttcgccac catgcggcac gccgcgtcgt actggcgggg gctgtccaag gcgggcgagg ccgtcgacgg ccggatcatc aacaccagct cgggcgccgg cctgcagggc agcgtcgggc agggcaacta cagcgccgcc aaggccggta tcgcgacgct gacgctggtc ggcgcggcgg agatgggccg ttacggcgtc accgtcaacg cgatcgcgcc gtcggcccgc acccggatga ccgagaccgt gttcgccgag atgatggcaa cccaggacca ggatttcgac gcgatggccc cggaaaacgt gtcgcccctg gtggtttggc tgggcagtgc ggaggcccgc gacgtgaccg gcaaggtgtt cgaggtcgag ggcggcaaga tccgggtcgc cgagggttgg gcgcacggcc cgcagatcga caagggcgcg aggtgggatc ccgccgagct ggggcccgtc gtcgccgacc tgctcgccaa ggcccgcccg ccggtcccgg tctacggcgc caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa