MypaA.01549.a

PpdK

CENTER ID: MypaA.01549.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01549.a.A1.GE29614 Full length( MypaA.01549.a ) 1 560
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.2830
RefSeq: NP_961598.1
UniProt: Q73WJ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPESAATPRR GCTTARTAPG SGAHRNASRD RDAAPTEQSV VLLDGTSSHP RELLGNKGHG IEVMRRHHLP VPPAFCLTTA VGLRYLADPA ATMDAVWDDV LDRIGWLQAQ TSRTFGRGPH PLLVSVRSGA TQSMPGMMDT LLNLGINDSV EQALAAEHGP AFARDTHRRF REMYRRIVGV ERPVPSDPYT QLRAGIEAVF ASWNSPRARA YRAHYGFADR HGTAVVVQAM VFGNHGPNSG AGAFFSRNPI TGDDEPFGEW LPGGQGDDVV SGSVDVEPIA ALRDEQPAVY DQLMAAARTL ERLDRDVQEI EFTVEDGALW LLQTRAAERS AQAAVRSALQ LRREGLIDDA ETLRRVTPAH VRSLLMPALQ PEIRLAAPLL AKGLPACPGV VSGTAYADVD EALHAAERGE PVILVRDHTR PEDVSGMLAA RGIVTEVGGA ASHAAVVSRE LGRVAVVGCG AGVAAMLAGR RITVDGAEGE VREGELSLSA WSENDTPELR ELAEIARLIS PLRAHSGGDH PRLDDDSESA VRAALAAGQT DVVSPTPLLV MLTALRQGAP
NT Sequence
TTGCCCGAAT CAGCAGCGAC ACCACGAAGG GGGTGCACCA CGGCTCGCAC CGCACCAGGT AGCGGCGCCC ACCGCAACGC GTCACGCGAC CGGGACGCCG CGCCGACCGA GCAGTCCGTG GTGTTGCTGG ACGGCACATC GAGCCACCCC CGCGAGCTGC TTGGCAACAA GGGCCACGGC ATCGAGGTGA TGCGCCGCCA CCACCTGCCG GTGCCGCCCG CGTTCTGCCT CACCACCGCG GTGGGCCTGC GGTATCTGGC CGACCCCGCG GCGACGATGG ACGCCGTCTG GGACGACGTG CTGGACCGGA TCGGCTGGCT GCAGGCGCAA ACCTCGCGCA CGTTCGGGCG GGGCCCGCAC CCGCTGCTGG TCAGCGTGCG CTCGGGGGCC ACCCAGTCGA TGCCCGGGAT GATGGACACC CTGTTGAACC TCGGCATCAA CGACTCCGTG GAGCAGGCGC TGGCCGCCGA GCACGGGCCG GCGTTCGCCC GCGACACCCA CCGGCGGTTC CGCGAGATGT ACCGGCGCAT CGTGGGTGTC GAGCGGCCGG TGCCGTCCGA CCCGTACACG CAGTTGCGCG CCGGCATCGA GGCGGTGTTC GCGTCGTGGA ACTCCCCGCG CGCCCGCGCC TACCGCGCCC ATTACGGTTT CGCCGACCGG CACGGCACCG CCGTCGTCGT GCAGGCCATG GTGTTCGGCA ATCACGGCCC CAATTCCGGT GCGGGAGCGT TCTTTTCGCG CAACCCGATC ACCGGGGACG ACGAGCCGTT CGGCGAGTGG CTGCCCGGGG GCCAGGGCGA CGACGTGGTG TCGGGATCGG TCGACGTGGA ACCGATCGCC GCGCTGCGCG ACGAGCAGCC GGCCGTCTAC GACCAGCTGA TGGCCGCCGC CCGGACGCTG GAGCGGTTGG ACCGTGACGT CCAGGAGATC GAGTTCACCG TCGAGGACGG CGCCCTGTGG TTGCTGCAGA CCCGGGCGGC CGAACGCTCG GCGCAGGCGG CGGTGCGCTC GGCGCTGCAG CTGCGACGCG AGGGGCTGAT CGACGACGCC GAGACGCTGC GGCGGGTCAC CCCGGCGCAC GTGCGGTCGC TGCTGATGCC CGCCCTGCAA CCCGAAATAC GTTTGGCGGC ACCACTTCTG GCCAAGGGCC TACCTGCCTG CCCCGGCGTG GTATCCGGCA CGGCCTACGC CGACGTCGAC GAGGCGCTGC ACGCCGCCGA GCGGGGCGAA CCGGTGATCC TGGTGCGCGA CCACACCCGG CCCGAAGACG TGTCGGGCAT GCTGGCCGCG CGGGGCATCG TCACCGAGGT GGGCGGCGCG GCCAGCCATG CGGCGGTGGT CAGCCGGGAA CTGGGCCGGG TGGCCGTGGT GGGTTGCGGC GCCGGGGTGG CCGCGATGCT GGCGGGCCGG CGGATCACCG TCGACGGCGC CGAAGGCGAA GTGCGCGAAG GCGAATTGTC CCTGTCCGCA TGGTCCGAGA ACGATACCCC GGAGCTGCGG GAGCTGGCCG AGATCGCGCG GCTGATCAGC CCGCTGCGCG CCCATTCCGG CGGCGACCAT CCGCGGCTGG ACGACGACTC CGAGAGCGCC GTGCGCGCGG CCCTGGCCGC CGGGCAGACC GACGTGGTCT CGCCCACCCC GCTGCTGGTC ATGCTGACCG CGCTGCGCCA GGGCGCGCCG
Details for MypaA.01549.a.A1.GE29614
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 62kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 69
PERCENT COVERAGE: 29
Validated AA Sequence
MXTXXAXTXG PGXMLPESAA TPRRGCTTAR TAXXSGXXRN ASRDRDAAPT EQSVVLLDGT SSHPRXLIGX XGHGIXVXHX HHLPVPPAFC LTTAVGLRYL ADPAATMXXX WDDVLDRIGW LQAQTSRTFG RGPHPLXXXX AXXSXXXXXX XXXXXXXXXX RXXXXSXXXX
Validated NT Sequence
ttgtttaact ttannannga gatatacnnt ggctcatcac catcaccatc ntatggntac ncngnaagct canacncnng gtcctggntn natgttgccc gaatcagcag cgacaccacg aagggggtgc accacggctc gcaccgcanc nngtagcggc ncannncgca acgcgtcncg cgaccgggac gccgcgccga ccgagcagtc cgtggtgttg ctggacggca catcnagcca cccccgcnag ctgattggnn ncannggcca cggcatcnag gtgangcacc nccaccacct gccggtgccg cccgcgttct gcctcaccac cgcggtgggc ctgcggtatc tggccgaccc cgcggcgacg atggangnnn nctgggacga cgtgctggac cggatcggct ggctgcaggc gcaaacctcg cgcacgttcg ggcggggccc gcacccgctg angnnnannn nngcncnngn ctcgncnnnn nnnnnancnt nnncnnnnnn nncanncnan nttcncnann ancgnntgnn annnnnctcn naannnannn cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLPESAATP RRGCTTARTA PGSGAHRNAS RDRDAAPTEQ SVVLLDGTSS HPRELLGNKG HGIEVMRRHH LPVPPAFCLT TAVGLRYLAD PAATMDAVWD DVLDRIGWLQ AQTSRTFGRG PHPLLVSVRS GATQSMPGMM DTLLNLGIND SVEQALAAEH GPAFARDTHR RFREMYRRIV GVERPVPSDP YTQLRAGIEA VFASWNSPRA RAYRAHYGFA DRHGTAVVVQ AMVFGNHGPN SGAGAFFSRN PITGDDEPFG EWLPGGQGDD VVSGSVDVEP IAALRDEQPA VYDQLMAAAR TLERLDRDVQ EIEFTVEDGA LWLLQTRAAE RSAQAAVRSA LQLRREGLID DAETLRRVTP AHVRSLLMPA LQPEIRLAAP LLAKGLPACP GVVSGTAYAD VDEALHAAER GEPVILVRDH TRPEDVSGML AARGIVTEVG GAASHAAVVS RELGRVAVVG CGAGVAAMLA GRRITVDGAE GEVREGELSL SAWSENDTPE LRELAEIARL ISPLRAHSGG DHPRLDDDSE SAVRAALAAG QTDVVSPTPL LVMLTALRQG AP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcccgaa tcagcagcga caccacgaag ggggtgcacc acggctcgca ccgcaccagg tagcggcgcc caccgcaacg cgtcacgcga ccgggacgcc gcgccgaccg agcagtccgt ggtgttgctg gacggcacat cgagccaccc ccgcgagctg cttggcaaca agggccacgg catcgaggtg atgcgccgcc accacctgcc ggtgccgccc gcgttctgcc tcaccaccgc ggtgggcctg cggtatctgg ccgaccccgc ggcgacgatg gacgccgtct gggacgacgt gctggaccgg atcggctggc tgcaggcgca aacctcgcgc acgttcgggc ggggcccgca cccgctgctg gtcagcgtgc gctcgggggc cacccagtcg atgcccggga tgatggacac cctgttgaac ctcggcatca acgactccgt ggagcaggcg ctggccgccg agcacgggcc ggcgttcgcc cgcgacaccc accggcggtt ccgcgagatg taccggcgca tcgtgggtgt cgagcggccg gtgccgtccg acccgtacac gcagttgcgc gccggcatcg aggcggtgtt cgcgtcgtgg aactccccgc gcgcccgcgc ctaccgcgcc cattacggtt tcgccgaccg gcacggcacc gccgtcgtcg tgcaggccat ggtgttcggc aatcacggcc ccaattccgg tgcgggagcg ttcttttcgc gcaacccgat caccggggac gacgagccgt tcggcgagtg gctgcccggg ggccagggcg acgacgtggt gtcgggatcg gtcgacgtgg aaccgatcgc cgcgctgcgc gacgagcagc cggccgtcta cgaccagctg atggccgccg cccggacgct ggagcggttg gaccgtgacg tccaggagat cgagttcacc gtcgaggacg gcgccctgtg gttgctgcag acccgggcgg ccgaacgctc ggcgcaggcg gcggtgcgct cggcgctgca gctgcgacgc gaggggctga tcgacgacgc cgagacgctg cggcgggtca ccccggcgca cgtgcggtcg ctgctgatgc ccgccctgca acccgaaata cgtttggcgg caccacttct ggccaagggc ctacctgcct gccccggcgt ggtatccggc acggcctacg ccgacgtcga cgaggcgctg cacgccgccg agcggggcga accggtgatc ctggtgcgcg accacacccg gcccgaagac gtgtcgggca tgctggccgc gcggggcatc gtcaccgagg tgggcggcgc ggccagccat gcggcggtgg tcagccggga actgggccgg gtggccgtgg tgggttgcgg cgccggggtg gccgcgatgc tggcgggccg gcggatcacc gtcgacggcg ccgaaggcga agtgcgcgaa ggcgaattgt ccctgtccgc atggtccgag aacgataccc cggagctgcg ggagctggcc gagatcgcgc ggctgatcag cccgctgcgc gcccattccg gcggcgacca tccgcggctg gacgacgact ccgagagcgc cgtgcgcgcg gccctggccg ccgggcagac cgacgtggtc tcgcccaccc cgctgctggt catgctgacc gcgctgcgcc agggcgcgcc gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa