MypaA.01365.a

Putative uncharacterized protein

CENTER ID: MypaA.01365.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01365.a.A1.GE29598 Full length( MypaA.01365.a ) 1 256
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01365.a.A1.PW29498 Full length( MypaA.01365.a ) 1 256

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.1741
RefSeq: NP_960583.1
UniProt: Q73ZF4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVLDLFDLR GRRALVTGAS SGIGKQVAQA YLQAGARVAI AARDFEALQR TADELTAGGT GGVVAIRCDV TQPDEVSGMV DRMIAELGGI DIAVCNAGII SVSPMLEMPA AEFQRIQDTN VTGVFLTAQA AARAMMRQGR GGAIITTASM SGHIINVPQQ VGHYCASKAA VIQLTKAMAV EFAPHDIRVN SVSPGYIRTE LVEPLHEYHR QWEPKIPLGR MGRPDELAGI YLYLASAASS YMTGSDIVID GGYSCP
NT Sequence
ATGAGCGTGT TGGATCTGTT CGACCTGCGC GGCAGGCGGG CGCTGGTCAC CGGGGCGTCC AGCGGGATCG GCAAGCAGGT GGCGCAGGCG TATCTGCAAG CCGGCGCTCG GGTGGCCATC GCGGCGCGCG ACTTCGAGGC GCTGCAACGC ACCGCGGACG AGCTCACCGC CGGCGGTACC GGCGGTGTGG TCGCGATCCG CTGCGACGTG ACCCAACCCG ATGAGGTGAG CGGCATGGTG GACCGGATGA TCGCCGAACT CGGCGGCATC GACATCGCGG TCTGCAATGC CGGCATCATC TCGGTCTCCC CGATGCTCGA GATGCCGGCC GCGGAATTCC AGCGCATCCA GGACACCAAC GTGACCGGGG TGTTTCTCAC CGCCCAGGCG GCGGCCCGGG CGATGATGCG GCAGGGCCGC GGCGGCGCCA TCATCACCAC CGCCTCGATG TCCGGGCACA TCATCAACGT CCCGCAGCAG GTCGGCCACT ACTGCGCCAG CAAGGCGGCC GTGATCCAGC TGACCAAGGC CATGGCCGTC GAATTCGCGC CGCACGACAT CCGGGTCAAC AGCGTCAGCC CCGGCTACAT CCGCACCGAG CTCGTCGAAC CGCTGCACGA ATACCATCGG CAGTGGGAGC CCAAGATCCC GCTCGGCCGG ATGGGCCGGC CGGACGAACT CGCCGGCATC TACCTGTATC TGGCCAGCGC GGCGTCCAGC TACATGACCG GTTCGGACAT CGTCATCGAC GGCGGATACA GCTGCCCG
Details for MypaA.01365.a.A1.GE29598
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 29kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 92
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSVLDLFDL RGRRALVTGA SSGIGKQVAQ AYLQAGARVA IAARDFEALQ RTADELTAGG TGGVVAIRCD VTQPDEVSGM VDRMIAELGG IDIAVCNAGI ISVSPMLEMP AAEFQRIQDT NVTGVFLTAQ AAARAMMRQG RGGAIITTAS MSGHIINVPQ QVGHYCASKA AVIQLTKAMA VEFAPHDIRV NSVSPGYIRT ELVEPLHEYH RQWEPKIPLG RMGRPDELAG IYLYLGQRGV QLHDRFGHRH RRRIQLPVI
Validated NT Sequence
ttattacggg cagctgtatc cgccgtcgat gacgatgtcc gaaccggtca tgtagctgga cgccgcgctg gcccagatac aggtagatgc cggcgagttc gtccggccgg cccatccggc cgagcgggat cttgggctcc cactgccgat ggtattcgtg cagcggttcg acgagctcgg tgcggatgta gccggggctg acgctgttga cccggatgtc gtgcggcgcg aattcgacgg ccatggcctt ggtcagctgg atcacggccg ccttgctggc gcagtagtgg ccgacctgct gcgggacgtt gatgatgtgc ccggacatcg aggcggtggt gatgatggcg ccgccgcggc cctgccgcat catcgcccgg gccgccgcct gggcggtgag aaacaccccg gtcacgttgg tgtcctggat gcgctggaat tccgcggccg gcatctcgag catcggggag accgagatga tgccggcatt gcagaccgcg atgtcgatgc cgccgagttc ggcgatcatc cggtccacca tgccgctcac ctcatcgggt tgggtcacgt cgcagcggat cgcgaccaca ccgccggtac cgccggcggt gagctcgtcc gcggtgcgtt gcagcgcctc gaagtcgcgc gccgcgatgg ccacccgagc gccggcttgc agatacgcct gcgccacctg cttgccgatc ccgctggacg ccccggtgac cagcgcccgc ctgccgcgca ggtcgaacag atccaacacg ctcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVLDLFDL RGRRALVTGA SSGIGKQVAQ AYLQAGARVA IAARDFEALQ RTADELTAGG TGGVVAIRCD VTQPDEVSGM VDRMIAELGG IDIAVCNAGI ISVSPMLEMP AAEFQRIQDT NVTGVFLTAQ AAARAMMRQG RGGAIITTAS MSGHIINVPQ QVGHYCASKA AVIQLTKAMA VEFAPHDIRV NSVSPGYIRT ELVEPLHEYH RQWEPKIPLG RMGRPDELAG IYLYLASAAS SYMTGSDIVI DGGYSCP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgtgttg gatctgttcg acctgcgcgg caggcgggcg ctggtcaccg gggcgtccag cgggatcggc aagcaggtgg cgcaggcgta tctgcaagcc ggcgctcggg tggccatcgc ggcgcgcgac ttcgaggcgc tgcaacgcac cgcggacgag ctcaccgccg gcggtaccgg cggtgtggtc gcgatccgct gcgacgtgac ccaacccgat gaggtgagcg gcatggtgga ccggatgatc gccgaactcg gcggcatcga catcgcggtc tgcaatgccg gcatcatctc ggtctccccg atgctcgaga tgccggccgc ggaattccag cgcatccagg acaccaacgt gaccggggtg tttctcaccg cccaggcggc ggcccgggcg atgatgcggc agggccgcgg cggcgccatc atcaccaccg cctcgatgtc cgggcacatc atcaacgtcc cgcagcaggt cggccactac tgcgccagca aggcggccgt gatccagctg accaaggcca tggccgtcga attcgcgccg cacgacatcc gggtcaacag cgtcagcccc ggctacatcc gcaccgagct cgtcgaaccg ctgcacgaat accatcggca gtgggagccc aagatcccgc tcggccggat gggccggccg gacgaactcg ccggcatcta cctgtatctg gccagcgcgg cgtccagcta catgaccggt tcggacatcg tcatcgacgg cggatacagc tgcccgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MypaA.01365.a.A1.PW29498
PURIFICATION DATe: 8/23/2010
CONCENTRATION: 32.03mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 92
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSVLDLFDL RGRRALVTGA SSGIGKQVAQ AYLQAGARVA IAARDFEALQ RTADELTAGG TGGVVAIRCD VTQPDEVSGM VDRMIAELGG IDIAVCNAGI ISVSPMLEMP AAEFQRIQDT NVTGVFLTAQ AAARAMMRQG RGGAIITTAS MSGHIINVPQ QVGHYCASKA AVIQLTKAMA VEFAPHDIRV NSVSPGYIRT ELVEPLHEYH RQWEPKIPLG RMGRPDELAG IYLYLGQRGV QLHDRFGHRH RRRIQLPVI
Validated NT Sequence
ttattacggg cagctgtatc cgccgtcgat gacgatgtcc gaaccggtca tgtagctgga cgccgcgctg gcccagatac aggtagatgc cggcgagttc gtccggccgg cccatccggc cgagcgggat cttgggctcc cactgccgat ggtattcgtg cagcggttcg acgagctcgg tgcggatgta gccggggctg acgctgttga cccggatgtc gtgcggcgcg aattcgacgg ccatggcctt ggtcagctgg atcacggccg ccttgctggc gcagtagtgg ccgacctgct gcgggacgtt gatgatgtgc ccggacatcg aggcggtggt gatgatggcg ccgccgcggc cctgccgcat catcgcccgg gccgccgcct gggcggtgag aaacaccccg gtcacgttgg tgtcctggat gcgctggaat tccgcggccg gcatctcgag catcggggag accgagatga tgccggcatt gcagaccgcg atgtcgatgc cgccgagttc ggcgatcatc cggtccacca tgccgctcac ctcatcgggt tgggtcacgt cgcagcggat cgcgaccaca ccgccggtac cgccggcggt gagctcgtcc gcggtgcgtt gcagcgcctc gaagtcgcgc gccgcgatgg ccacccgagc gccggcttgc agatacgcct gcgccacctg cttgccgatc ccgctggacg ccccggtgac cagcgcccgc ctgccgcgca ggtcgaacag atccaacacg ctcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVLDLFDL RGRRALVTGA SSGIGKQVAQ AYLQAGARVA IAARDFEALQ RTADELTAGG TGGVVAIRCD VTQPDEVSGM VDRMIAELGG IDIAVCNAGI ISVSPMLEMP AAEFQRIQDT NVTGVFLTAQ AAARAMMRQG RGGAIITTAS MSGHIINVPQ QVGHYCASKA AVIQLTKAMA VEFAPHDIRV NSVSPGYIRT ELVEPLHEYH RQWEPKIPLG RMGRPDELAG IYLYLASAAS SYMTGSDIVI DGGYSCP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGTGTTG GATCTGTTCG ACCTGCGCGG CAGGCGGGCG CTGGTCACCG GGGCGTCCAG CGGGATCGGC AAGCAGGTGG CGCAGGCGTA TCTGCAAGCC GGCGCTCGGG TGGCCATCGC GGCGCGCGAC TTCGAGGCGC TGCAACGCAC CGCGGACGAG CTCACCGCCG GCGGTACCGG CGGTGTGGTC GCGATCCGCT GCGACGTGAC CCAACCCGAT GAGGTGAGCG GCATGGTGGA CCGGATGATC GCCGAACTCG GCGGCATCGA CATCGCGGTC TGCAATGCCG GCATCATCTC GGTCTCCCCG ATGCTCGAGA TGCCGGCCGC GGAATTCCAG CGCATCCAGG ACACCAACGT GACCGGGGTG TTTCTCACCG CCCAGGCGGC GGCCCGGGCG ATGATGCGGC AGGGCCGCGG CGGCGCCATC ATCACCACCG CCTCGATGTC CGGGCACATC ATCAACGTCC CGCAGCAGGT CGGCCACTAC TGCGCCAGCA AGGCGGCCGT GATCCAGCTG ACCAAGGCCA TGGCCGTCGA ATTCGCGCCG CACGACATCC GGGTCAACAG CGTCAGCCCC GGCTACATCC GCACCGAGCT CGTCGAACCG CTGCACGAAT ACCATCGGCA GTGGGAGCCC AAGATCCCGC TCGGCCGGAT GGGCCGGCCG GACGAACTCG CCGGCATCTA CCTGTATCTG GCCAGCGCGG CGTCCAGCTA CATGACCGGT TCGGACATCG TCATCGACGG CGGATACAGC TGCCCGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA