SSGCID: Target Page

MypaA.01326.h

Putative uncharacterized protein

CENTER ID:	MypaA.01326.h
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01326.h.A1.GE29579	Full length( MypaA.01326.h )		1	299

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01326.h.A1.PS01044	Full length( MypaA.01326.h )		1	299

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.1972
RefSeq:	NP_960795.1
UniProt:	Q73YU4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSLRDKVVF ITGGARGIGA EVARRLKAKG ARLVLTDLND AELTSLAAEL GEERVLTAVA DVRDLSAMQA AADRAVERFG GIDVVLANAG IASYGSVAQV DPDAFRRVLD INVLGVFHTV RATLPAVIER RGYVLIVSSL AAYAACPGLA PYNASKAGVE MLANALRLEV ARHGVKVGSA HMSWINTALV RDTQNDLPAF DRLLASLPWP LNKTTTVDKC AAAFVKGIQR RRARIYCPRW VALFRWAKPV LSSRLGEMPL HSPTAELLPA LDAQVAALGR SMSAANVELL RPDRGSARN

NT Sequence

ATGACATCGC TGCGGGACAA AGTCGTCTTC ATCACCGGCG GCGCCCGGGG AATCGGCGCC GAGGTCGCCC GCCGGCTGAA AGCGAAAGGC GCCCGGTTGG TGCTGACCGA CCTCAACGAC GCCGAACTGA CCTCGCTTGC CGCGGAACTC GGCGAGGAGC GGGTGCTGAC GGCCGTCGCG GACGTGCGCG ATCTGTCGGC CATGCAGGCC GCCGCCGACC GCGCCGTCGA GCGATTCGGT GGCATCGACG TGGTGCTGGC CAACGCCGGC ATCGCCAGCT ACGGCTCGGT GGCCCAGGTC GACCCGGACG CGTTCAGGCG AGTCCTCGAC ATCAACGTGC TCGGCGTCTT TCACACCGTG CGAGCGACCT TGCCCGCGGT GATCGAGCGC CGCGGCTACG TGCTGATCGT CTCGTCGCTG GCCGCCTACG CGGCCTGCCC GGGGCTGGCC CCCTACAACG CGTCGAAGGC CGGCGTCGAG ATGCTGGCCA ACGCGCTGCG ACTGGAAGTG GCCCGGCACG GCGTCAAGGT CGGCTCGGCG CACATGTCCT GGATCAACAC CGCGTTGGTT CGCGACACCC AGAACGACCT GCCGGCCTTC GATCGACTGC TCGCCAGCCT GCCCTGGCCG CTGAACAAGA CCACGACGGT CGACAAATGC GCGGCCGCGT TCGTCAAGGG CATCCAGCGC CGCCGCGCCC GCATCTACTG CCCACGGTGG GTGGCGCTGT TCCGCTGGGC CAAGCCGGTG CTGTCCAGTC GGCTCGGCGA AATGCCGCTG CACTCACCGA CCGCCGAGCT GTTGCCGGCA CTGGACGCCC AAGTCGCCGC GCTGGGCCGT TCGATGTCGG CCGCCAACGT GGAGTTGTTG CGGCCGGATC GCGGGTCGGC GCGCAAC

Details for MypaA.01326.h.A1.GE29579

HARVESTED ON:	6/15/2010
SEQUENCED ON:	6/27/2010
EXPECTED MW:	34kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMTSLRDK VVFITGGARG IGAEVARRLK AKGARLVLTD LNDAELTSLA AELGEERVLT AVADVRDLSA MQAAADRAVE RFGGIDVVLA NAGIASYGSV AQVDPDAFRR VLDINVLGVF HTVRATLPAV IERRGYVLIV SSLAAYAACP GLAPYNASKA GVEMLANALR LEVARHGVKV GSAHMSWINT ALVRDTQNDL PAFDRLLASL PWPLNKTTTV DKCAAAFVKG IQRRRARIYC PRWVALFRWA KPVLSSRLGE MPLHSPTAEL LPALDAQVAA LGRSMSAANV ELLRPDRGSA RN

Validated NT Sequence

ttattagttg cgcgccgacc cgcgatccgg ccgcaacaac tccacgttgg cggccgacat cgaacggccc agcgcggcga cttgggcgtc cagtgccggc aacagctcgg cggtcggtga gtgcagcggc atttcgccga gccgactgga cagcaccggc ttggcccagc ggaacagcgc cacccaccgt gggcagtaga tgcgggcgcg gcggcgctgg atgcccttga cgaacgcggc cgcgcatttg tcgaccgtcg tggtcttgtt cagcggccag ggcaggctgg cgagcagtcg atcgaaggcc ggcaggtcgt tctgggtgtc gcgaaccaac gcggtgttga tccaggacat gtgcgccgag ccgaccttga cgccgtgccg ggccacttcc agtcgcagcg cgttggccag catctcgacg ccggccttcg acgcgttgta gggggccagc cccgggcagg ccgcgtaggc ggccagcgac gagacgatca gcacgtagcc gcggcgctcg atcaccgcgg gcaaggtcgc tcgcacggtg tgaaagacgc cgagcacgtt gatgtcgagg actcgcctga acgcgtccgg gtcgacctgg gccaccgagc cgtagctggc gatgccggcg ttggccagca ccacgtcgat gccaccgaat cgctcgacgg cgcggtcggc ggcggcctgc atggccgaca gatcgcgcac gtccgcgacg gccgtcagca cccgctcctc gccgagttcc gcggcaagcg aggtcagttc ggcgtcgttg aggtcggtca gcaccaaccg ggcgcctttc gctttcagcc ggcgggcgac ctcggcgccg attccccggg cgccgccggt gatgaagacg actttgtccc gcagcgatgt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTSLRDKVV FITGGARGIG AEVARRLKAK GARLVLTDLN DAELTSLAAE LGEERVLTAV ADVRDLSAMQ AAADRAVERF GGIDVVLANA GIASYGSVAQ VDPDAFRRVL DINVLGVFHT VRATLPAVIE RRGYVLIVSS LAAYAACPGL APYNASKAGV EMLANALRLE VARHGVKVGS AHMSWINTAL VRDTQNDLPA FDRLLASLPW PLNKTTTVDK CAAAFVKGIQ RRRARIYCPR WVALFRWAKP VLSSRLGEMP LHSPTAELLP ALDAQVAALG RSMSAANVEL LRPDRGSARN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacatcgctg cgggacaaag tcgtcttcat caccggcggc gcccggggaa tcggcgccga ggtcgcccgc cggctgaaag cgaaaggcgc ccggttggtg ctgaccgacc tcaacgacgc cgaactgacc tcgcttgccg cggaactcgg cgaggagcgg gtgctgacgg ccgtcgcgga cgtgcgcgat ctgtcggcca tgcaggccgc cgccgaccgc gccgtcgagc gattcggtgg catcgacgtg gtgctggcca acgccggcat cgccagctac ggctcggtgg cccaggtcga cccggacgcg ttcaggcgag tcctcgacat caacgtgctc ggcgtctttc acaccgtgcg agcgaccttg cccgcggtga tcgagcgccg cggctacgtg ctgatcgtct cgtcgctggc cgcctacgcg gcctgcccgg ggctggcccc ctacaacgcg tcgaaggccg gcgtcgagat gctggccaac gcgctgcgac tggaagtggc ccggcacggc gtcaaggtcg gctcggcgca catgtcctgg atcaacaccg cgttggttcg cgacacccag aacgacctgc cggccttcga tcgactgctc gccagcctgc cctggccgct gaacaagacc acgacggtcg acaaatgcgc ggccgcgttc gtcaagggca tccagcgccg ccgcgcccgc atctactgcc cacggtgggt ggcgctgttc cgctgggcca agccggtgct gtccagtcgg ctcggcgaaa tgccgctgca ctcaccgacc gccgagctgt tgccggcact ggacgcccaa gtcgccgcgc tgggccgttc gatgtcggcc gccaacgtgg agttgttgcg gccggatcgc gggtcggcgc gcaacaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for MypaA.01326.h.A1.PS01044

PURIFICATION DATe:	4/14/2011
CONCENTRATION:	27.3mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACATCGCTG CGGGACAAAG TCGTCTTCAT CACCGGCGGC GCCCGGGGAA TCGGCGCCGA GGTCGCCCGC CGGCTGAAAG CGAAAGGCGC CCGGTTGGTG CTGACCGACC TCAACGACGC CGAACTGACC TCGCTTGCCG CGGAACTCGG CGAGGAGCGG GTGCTGACGG CCGTCGCGGA CGTGCGCGAT CTGTCGGCCA TGCAGGCCGC CGCCGACCGC GCCGTCGAGC GATTCGGTGG CATCGACGTG GTGCTGGCCA ACGCCGGCAT CGCCAGCTAC GGCTCGGTGG CCCAGGTCGA CCCGGACGCG TTCAGGCGAG TCCTCGACAT CAACGTGCTC GGCGTCTTTC ACACCGTGCG AGCGACCTTG CCCGCGGTGA TCGAGCGCCG CGGCTACGTG CTGATCGTCT CGTCGCTGGC CGCCTACGCG GCCTGCCCGG GGCTGGCCCC CTACAACGCG TCGAAGGCCG GCGTCGAGAT GCTGGCCAAC GCGCTGCGAC TGGAAGTGGC CCGGCACGGC GTCAAGGTCG GCTCGGCGCA CATGTCCTGG ATCAACACCG CGTTGGTTCG CGACACCCAG AACGACCTGC CGGCCTTCGA TCGACTGCTC GCCAGCCTGC CCTGGCCGCT GAACAAGACC ACGACGGTCG ACAAATGCGC GGCCGCGTTC GTCAAGGGCA TCCAGCGCCG CCGCGCCCGC ATCTACTGCC CACGGTGGGT GGCGCTGTTC CGCTGGGCCA AGCCGGTGCT GTCCAGTCGG CTCGGCGAAA TGCCGCTGCA CTCACCGACC GCCGAGCTGT TGCCGGCACT GGACGCCCAA GTCGCCGCGC TGGGCCGTTC GATGTCGGCC GCCAACGTGG AGTTGTTGCG GCCGGATCGC GGGTCGGCGC GCAACAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA