SSGCID: Target Page

MypaA.01285.a

Pnp

CENTER ID:	MypaA.01285.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01285.a.A1.GE29571	Full length( MypaA.01285.a )		1	260

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.4292
RefSeq:	NP_962964.1
UniProt:	Q73SP3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHHNGRMLGV IGGSGFYTFF GSDADDVTVD TPYGPPSAPV TVGAVGGHRV AFLPRHEFSA HTVPYRANLW ALRKLGVRRV LAPCAVGSLV PELGPGAVVV PDQLVDRTRG RADTYFDSGA VHVDFADPYC PALREAVTGL PGVVDGGTMV VIQGPRFSTR AESRWFASAG FSLVNMTGYP EAVLARELEI CYAAIALVTD LDAGVSAGEG VKAVEVFAEF EKNIEPFKKL VRDAIGRVAA ERSCTQCSPH TGVSLPIELP

NT Sequence

ATGCACCACA ATGGTCGGAT GCTCGGAGTC ATCGGCGGCA GCGGCTTCTA CACCTTCTTC GGATCCGACG CCGACGACGT CACCGTGGAT ACCCCGTACG GGCCGCCCAG CGCCCCGGTC ACCGTCGGCG CCGTCGGCGG GCACCGGGTG GCCTTCCTGC CCCGGCACGA ATTCTCCGCG CACACCGTGC CGTACCGGGC CAACCTGTGG GCGCTGCGCA AACTCGGTGT GCGCCGGGTG CTGGCGCCGT GCGCGGTCGG CAGCCTGGTG CCCGAGCTCG GCCCGGGCGC CGTCGTGGTG CCCGACCAGC TGGTCGACCG CACCCGCGGG CGCGCCGACA CCTATTTCGA CTCCGGGGCC GTGCACGTCG ACTTCGCCGA CCCGTACTGC CCGGCGCTGC GCGAGGCGGT GACCGGGCTG CCGGGGGTGG TCGATGGCGG CACCATGGTG GTGATCCAGG GGCCGCGGTT TTCCACCCGC GCCGAGAGCC GGTGGTTCGC CTCGGCCGGG TTCTCGCTGG TGAACATGAC CGGCTACCCG GAGGCGGTGC TCGCCCGGGA GCTTGAAATA TGTTATGCGG CAATCGCTTT GGTCACAGAT CTGGATGCCG GTGTGAGCGC CGGTGAGGGA GTGAAGGCGG TTGAGGTGTT CGCCGAGTTC GAGAAGAACA TCGAGCCGTT CAAGAAGCTG GTGCGCGACG CCATCGGCCG GGTCGCCGCC GAGCGCAGCT GTACGCAGTG CTCACCGCAC ACCGGGGTGA GCCTGCCGAT CGAGCTGCCA

Details for MypaA.01285.a.A1.GE29571

HARVESTED ON:	6/15/2010
SEQUENCED ON:	6/27/2010
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMHHNGRMLG VIGGSGFYTF FGSDADDVTV DTPYGPPSAP VTVGAVGGHR VAFLPRHEFS AHTVPYRANL WALRKLGVRR VLAPCAVGSL VPELGPGAVV VPDQLVDRTR GRADTYFDSG AVHVDFADPY CPALREAVTG LPGVVDGGTM VVIQGPRFST RAESRWFASA GFSLVNMTGY PEAVLARELE ICYAAIALVT DLDAGVSAGE GVKAVEVFAE FEKNIEPFKK LVRDAIGRVA AERSCTQCSP HTGVSLPIEL P

Validated NT Sequence

attttgttnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcacc acaatggtcg gatgctcgga gtcatcggcg gcagcggctt ctacaccttc ttcggatccg acgccgacga cgtcaccgtg gataccccgt acgggccgcc cagcgccccg gtcaccgtcg gcgccgtcgg cgggcaccgg gtggccttcc tgccccggca cgaattctcc gcgcacaccg tgccgtaccg ggccaacctg tgggcgctgc gcaaactcgg tgtgcgccgg gtgctggcgc cgtgcgcggt cggcagcctg gtgcccgagc tcggcccggg cgccgtcgtg gtgcccgacc agctggtcga ccgcacccgc gggcgcgccg acacctattt cgactccggg gccgtgcacg tcgacttcgc cgacccgtac tgcccggcgc tgcgcgaggc ggtgaccggg ctgccggggg tggtcgatgg cggcaccatg gtggtgatcc aggggccgcg gttttccacc cgcgccgaga gccggtggtt cgcctcggcc gggttctcgc tggtgaacat gaccggctac ccggaggcgg tgctcgcccg ggagcttgaa atatgttatg cggcaatcgc tttggtcaca gatctggatg ccggtgtgag cgccggtgag ggagtgaagg cggttgaggt gttcgccgag ttcgagaaga acatcgagcc gttcaagaag ctggtgcgcg acgccatcgg ccgggtcgcc gccgagcgca gctgtacgca gtgctcaccg cacaccgggg tgagcctgcc gatcgagctg ccataataaa cagcacgaac aagntctgca gccaagcttc tcgaggatcc nnctgctaac aaagcccgaa aggnagctga nttggctgct gcnnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaccacaat ggtcggatgc tcggagtcat cggcggcagc ggcttctaca ccttcttcgg atccgacgcc gacgacgtca ccgtggatac cccgtacggg ccgcccagcg ccccggtcac cgtcggcgcc gtcggcgggc accgggtggc cttcctgccc cggcacgaat tctccgcgca caccgtgccg taccgggcca acctgtgggc gctgcgcaaa ctcggtgtgc gccgggtgct ggcgccgtgc gcggtcggca gcctggtgcc cgagctcggc ccgggcgccg tcgtggtgcc cgaccagctg gtcgaccgca cccgcgggcg cgccgacacc tatttcgact ccggggccgt gcacgtcgac ttcgccgacc cgtactgccc ggcgctgcgc gaggcggtga ccgggctgcc gggggtggtc gatggcggca ccatggtggt gatccagggg ccgcggtttt ccacccgcgc cgagagccgg tggttcgcct cggccgggtt ctcgctggtg aacatgaccg gctacccgga ggcggtgctc gcccgggagc ttgaaatatg ttatgcggca atcgctttgg tcacagatct ggatgccggt gtgagcgccg gtgagggagt gaaggcggtt gaggtgttcg ccgagttcga gaagaacatc gagccgttca agaagctggt gcgcgacgcc atcggccggg tcgccgccga gcgcagctgt acgcagtgct caccgcacac cggggtgagc ctgccgatcg agctgccaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa