MypaA.01263.a

Putative uncharacterized protein

CENTER ID: MypaA.01263.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01263.a.A1.GE29569 Full length( MypaA.01263.a ) 1 373
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.2749
RefSeq: NP_961522.1
UniProt: Q73WS2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFSFTEEQE TIGKLARDLF ERRATPERLT ELEVGDTRHD AALWEELAAA DLLGAALPEA VGGSCASGGG FVELGVLLAE VGYGVAPVPA YATLVLGADS IARHGSSEQQ QRVLPGVIAG SRILTAGLAE PGRSDPARPA TTARRDGPNW RLDGAKELVP AAQLADTMLI PAVLDDGDVG LFLLAADAAG VEIRPVPTTN REPHADVFLA GARVSETDRI AGIGLVESLL TRALVALCAI QLGVVERALR IAAKYTTGRE QFGRPIGSFQ AVQQRMADAF IDVEAIRWTT WQAAWLVAHG RPADRAARIA KFWAAEAGAR VAATAQHVHG GIGIDVTYPL HRYFLWAKHN ELTLGPASAQ LAHLGATYSE GHA
NT Sequence
ATGGACTTCT CGTTCACCGA AGAGCAGGAG ACGATCGGCA AGCTGGCCCG CGATCTGTTC GAGCGCCGCG CCACCCCGGA ACGGCTGACC GAGTTGGAGG TCGGCGACAC CCGCCACGAC GCCGCGCTCT GGGAAGAGCT GGCCGCCGCC GACCTGCTCG GTGCCGCGCT GCCGGAGGCG GTCGGAGGCT CATGTGCTTC AGGCGGCGGC TTCGTGGAGC TCGGGGTGCT GCTGGCGGAG GTGGGCTACG GCGTCGCGCC GGTGCCCGCT TACGCCACGC TGGTGTTGGG CGCCGACTCG ATCGCCCGGC ACGGCAGCTC AGAACAGCAG CAGCGGGTCT TGCCGGGCGT GATCGCCGGA TCGCGCATCC TGACAGCGGG TTTGGCCGAA CCCGGTCGTT CGGATCCGGC CCGCCCGGCC ACCACCGCGC GTCGCGACGG CCCGAACTGG CGCCTCGACG GCGCCAAGGA GTTGGTGCCG GCCGCCCAAC TCGCCGACAC GATGCTGATT CCCGCCGTCC TGGACGACGG CGACGTGGGC CTGTTCCTGC TGGCCGCCGA CGCCGCCGGC GTCGAGATCC GCCCGGTGCC CACCACGAAT CGCGAACCGC ACGCGGACGT ATTCTTAGCC GGCGCACGTG TTTCCGAGAC GGACCGGATC GCCGGCATTG GACTGGTCGA GTCACTGCTC ACCCGGGCGC TGGTGGCGCT GTGCGCGATC CAGCTCGGCG TCGTCGAGCG GGCGCTGCGC ATCGCGGCCA AATACACCAC GGGCCGTGAG CAATTCGGCC GCCCGATCGG CAGCTTCCAG GCGGTGCAGC AGCGGATGGC CGACGCCTTC ATCGACGTCG AGGCGATCCG GTGGACCACC TGGCAGGCGG CCTGGCTGGT CGCCCACGGC CGGCCCGCCG ACCGGGCGGC GCGCATCGCG AAGTTCTGGG CCGCCGAGGC CGGCGCTCGG GTGGCCGCCA CCGCCCAGCA CGTCCACGGC GGCATCGGCA TCGACGTCAC CTATCCCCTG CACCGCTATT TCCTGTGGGC CAAGCACAAC GAGCTCACCC TCGGTCCGGC TTCGGCGCAG CTGGCCCACC TCGGCGCAAC CTATTCGGAA GGACACGCA
Details for MypaA.01263.a.A1.GE29569
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 41kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 93
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDFSFTEEQ ETIGKLARDL FERRATPERL TELEVGDTRH DAALWEELAA ADLLGAALPE AVGGSCASGG GFVELGVLLA EVGYGVAPVP AYATLVLGAD SIARHGSSEQ QQRVLPGVIA GSRILTAGLA EPGRSDPARP ATTARRDGPN WRLDGAKELV PAAQLADTML IPAVLDDGDV GLFLLAADAA GVEIRPVPTT NREPHADVFL AGARVSETDR IAGIGLVESL LTRALVALCA IQLGVVERAL RIAAKYTTGR EQXXRPIGSF QXVQQRXXRX XHRRRXDPVX XXXXGXXGXP XXXXRXGGXH XXXWXXXLXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
attttgttta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggact tctcgttcac cgaagagcag gagacgatcg gcaagctggc ccgcgatctg ttcgagcgcc gcgccacccc ggaacggctg accgagttgg aggtcggcga cacccgccac gacgccgcgc tctgggaaga gctggccgcc gccgacctgc tcggtgccgc gctgccggag gcggtcggag gctcatgtgc ttcaggcggc ggcttcgtgg agctcggggt gctgctggcg gaggtgggct acggcgtcgc gccggtgccc gcttacgcca cgctggtgtt gggcgccgac tcgatcgccc ggcacggcag ctcagaacag cagcagcggg tcttgccggg cgtgatcgcc ggatcgcgca tcctgacagc gggtttggcc gaacccggtc gttcggatcc ggcccgcccg gccaccaccg cgcgtcgcga cggcccgaac tggcgcctcg acggcgccaa ggagttggtg ccggccgccc aactcgccga cacgatgctg attcccgccg tcctggacga cggcgacgtg ggcctgttcc tgctggccgc cgacgccgcc ggcgtcgaga tccgcccggt gcccaccacg aatcgcgaac cgcacgcgga cgtattctta gccggcgcac gtgtttccga gacggaccgg atcgccggca ttggactggt cgagtcactg ctcacccggg cgctggtggc gctgtgcgcg atccagctcg gcgtcgtcga gcgggcgctg cgcatcgcgg ccaaatacac cacgggccgn gagcaattnn nccgcccgat cggcagcttc caggnggtgc agcagcggan ggnccgacnn nttcatcgac gtcgannnga tccngtggnn naccnngnnn gnggcntggn tggncnccca cngnnngncc nccgannngg cggngngcat cnnnantnnt ggnnannnnn gctcnggnnn nnncnncncc annnnntcnn ggnnnnttnn angtnncnnt nnnnnnnncn nnnnntnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDFSFTEEQ ETIGKLARDL FERRATPERL TELEVGDTRH DAALWEELAA ADLLGAALPE AVGGSCASGG GFVELGVLLA EVGYGVAPVP AYATLVLGAD SIARHGSSEQ QQRVLPGVIA GSRILTAGLA EPGRSDPARP ATTARRDGPN WRLDGAKELV PAAQLADTML IPAVLDDGDV GLFLLAADAA GVEIRPVPTT NREPHADVFL AGARVSETDR IAGIGLVESL LTRALVALCA IQLGVVERAL RIAAKYTTGR EQFGRPIGSF QAVQQRMADA FIDVEAIRWT TWQAAWLVAH GRPADRAARI AKFWAAEAGA RVAATAQHVH GGIGIDVTYP LHRYFLWAKH NELTLGPASA QLAHLGATYS EGHA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacttctcg ttcaccgaag agcaggagac gatcggcaag ctggcccgcg atctgttcga gcgccgcgcc accccggaac ggctgaccga gttggaggtc ggcgacaccc gccacgacgc cgcgctctgg gaagagctgg ccgccgccga cctgctcggt gccgcgctgc cggaggcggt cggaggctca tgtgcttcag gcggcggctt cgtggagctc ggggtgctgc tggcggaggt gggctacggc gtcgcgccgg tgcccgctta cgccacgctg gtgttgggcg ccgactcgat cgcccggcac ggcagctcag aacagcagca gcgggtcttg ccgggcgtga tcgccggatc gcgcatcctg acagcgggtt tggccgaacc cggtcgttcg gatccggccc gcccggccac caccgcgcgt cgcgacggcc cgaactggcg cctcgacggc gccaaggagt tggtgccggc cgcccaactc gccgacacga tgctgattcc cgccgtcctg gacgacggcg acgtgggcct gttcctgctg gccgccgacg ccgccggcgt cgagatccgc ccggtgccca ccacgaatcg cgaaccgcac gcggacgtat tcttagccgg cgcacgtgtt tccgagacgg accggatcgc cggcattgga ctggtcgagt cactgctcac ccgggcgctg gtggcgctgt gcgcgatcca gctcggcgtc gtcgagcggg cgctgcgcat cgcggccaaa tacaccacgg gccgtgagca attcggccgc ccgatcggca gcttccaggc ggtgcagcag cggatggccg acgccttcat cgacgtcgag gcgatccggt ggaccacctg gcaggcggcc tggctggtcg cccacggccg gcccgccgac cgggcggcgc gcatcgcgaa gttctgggcc gccgaggccg gcgctcgggt ggccgccacc gcccagcacg tccacggcgg catcggcatc gacgtcacct atcccctgca ccgctatttc ctgtgggcca agcacaacga gctcaccctc ggtccggctt cggcgcagct ggcccacctc ggcgcaacct attcggaagg acacgcaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa