MypaA.01249.c
Putative uncharacterized protein
| CENTER ID: | MypaA.01249.c |
| ORGANISM: | Mycobacterium paratuberculosis ATCC BAA-968 / K-10 |
| ASSOCIATED DISEASE: | Johne's disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.01249.c.A1.GE29567 | Full length( MypaA.01249.c ) | 1 | 289 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.01249.c.A1.PW29465 | Full length( MypaA.01249.c ) | 1 | 289 |
Structures
3RD5
DEPOSITED: 3/31/2011
DETERMINATION: XRay
CLONE: MypaA.01249.c.A1.GE29567
PROTEIN: MypaA.01249.c.A1.PW29465
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126621 |
| PATRIC ID: | fig|262316.17.peg.1025 |
| RefSeq: | NP_959914.1 |
| UniProt: | Q741V7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGWTAADLP SFAQRTVVIT GANSGLGAVT ARELARRGAT VIMAVRDTRK GEAAARTMAG QVEVRELDLQ DLSSVRRFAD GVSGADVLIN NAGIMAVPYA LTVDGFESQI GTNHLGHFAL TNLLLPRLTD RVVTVSSMAH WPGRINLEDL NWRSRRYSPW LAYSQSKLAN LLFTSELQRR LTAAGSPLRA LAAHPGYSHT NLQGASGRKL GDALMSAATR VVATDADFGA RQTLYAASQD LPGDSFVGPR FGYLGRTQPV GRSRRAKDAG MAAALWALSE QLTKTEFPL
NT Sequence
ATGACAGGCT GGACCGCCGC AGACCTACCG TCGTTCGCGC AGCGAACCGT CGTCATCACC GGCGCCAACA GCGGGTTGGG TGCGGTGACC GCCCGCGAGC TGGCGCGACG GGGGGCCACC GTCATCATGG CCGTGCGCGA CACCCGCAAG GGCGAGGCGG CCGCGCGGAC GATGGCCGGC CAGGTCGAGG TCCGCGAACT CGACCTGCAG GATCTGTCGT CGGTGCGCCG GTTCGCCGAC GGGGTGAGCG GGGCCGACGT GCTGATCAAC AATGCCGGCA TCATGGCCGT CCCGTACGCG CTGACCGTCG ACGGCTTCGA GAGCCAGATC GGCACCAACC ACCTGGGCCA TTTCGCGCTG ACCAACCTGC TGCTGCCCCG GCTCACCGAC CGCGTCGTGA CGGTGTCGTC GATGGCGCAC TGGCCGGGAC GGATCAACCT CGAGGATCTG AACTGGCGGT CGCGCCGGTA CTCGCCGTGG CTGGCCTACA GCCAGTCCAA GCTGGCCAAC CTGCTGTTCA CCAGCGAGCT GCAGCGCCGC CTGACGGCCG CCGGTTCCCC GCTGCGCGCG CTGGCCGCCC ACCCCGGCTA CTCGCACACC AACCTGCAGG GCGCCTCGGG CCGCAAGCTG GGCGACGCGC TGATGTCGGC GGCGACGCGG GTGGTCGCCA CCGACGCCGA TTTCGGCGCC CGGCAAACCC TGTACGCGGC GTCCCAGGAT CTGCCCGGGG ACAGCTTCGT CGGCCCCCGG TTCGGTTACC TGGGCCGCAC CCAACCGGTC GGACGCAGCC GGCGGGCCAA AGATGCCGGC ATGGCGGCGG CGCTGTGGGC GCTCTCCGAG CAGCTCACCA AGACCGAATT TCCGCTC
Details for MypaA.01249.c.A1.GE29567
| HARVESTED ON: | 6/15/2010 |
| SEQUENCED ON: | 6/27/2010 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MGTLEAQTQG PGSMTGWTAA DLPSFAQRTV VITGANSGLG AVTARELARR GATVIMAVRD TRKGEAAART MAGQVEVREL DLQDLSSVRR FADGVSGADV LINNAGIMAV PYALTVDGFE SQIGTNHLGH FALTNLLLPR LTDRVVTVSS MAHWPGRINL EDLNWRSRRY SPWLAYSQSK LANLLFTSEL QRRLTAAGSP LRALAAHPGY SHTNLQGASG RKLGDALMSA ATRVVATDAD FGARQTLYAA SQDLPGDSFV GPRFGYLGRT QPVGRSRRAK DAGMAAALWA LSEQLTKTEF PL
Validated NT Sequence
ttattagagc ggaaattcgg tcttggtgag ctgctcggag agcgcccaca gcgccgccgc catgccggca tctttggccc gccggctgcg tccgaccggt tgggtgcggc ccaggtaacc gaaccggggg ccgacgaagc tgtccccggg cagatcctgg gacgccgcgt acagggtttg ccgggcgccg aaatcggcgt cggtggcgac cacccgcgtc gccgccgaca tcagcgcgtc gcccagcttg cggcccgagg cgccctgcag gttggtgtgc gagtagccgg ggtgggcggc cagcgcgcgc agcggggaac cggcggccgt caggcggcgc tgcagctcgc tggtgaacag caggttggcc agcttggact ggctgtaggc cagccacggc gagtaccggc gcgaccgcca gttcagatcc tcgaggttga tccgtcccgg ccagtgcgcc atcgacgaca ccgtcacgac gcggtcggtg agccggggca gcagcaggtt ggtcagcgcg aaatggccca ggtggttggt gccgatctgg ctctcgaagc cgtcgacggt cagcgcgtac gggacggcca tgatgccggc attgttgatc agcacgtcgg ccccgctcac cccgtcggcg aaccggcgca ccgacgacag atcctgcagg tcgagttcgc ggacctcgac ctggccggcc atcgtccgcg cggccgcctc gcccttgcgg gtgtcgcgca cggccatgat gacggtggcc ccccgtcgcg ccagctcgcg ggcggtcacc gcacccaacc cgctgttggc gccggtgatg acgacggttc gctgcgcgaa cgacggtagg tctgcggcgg tccagcctgt catcgaacca ggaccctggg tctgagcttc cagggtaccc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTGWTAADL PSFAQRTVVI TGANSGLGAV TARELARRGA TVIMAVRDTR KGEAAARTMA GQVEVRELDL QDLSSVRRFA DGVSGADVLI NNAGIMAVPY ALTVDGFESQ IGTNHLGHFA LTNLLLPRLT DRVVTVSSMA HWPGRINLED LNWRSRRYSP WLAYSQSKLA NLLFTSELQR RLTAAGSPLR ALAAHPGYSH TNLQGASGRK LGDALMSAAT RVVATDADFG ARQTLYAASQ DLPGDSFVGP RFGYLGRTQP VGRSRRAKDA GMAAALWALS EQLTKTEFPL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaggctgg accgccgcag acctaccgtc gttcgcgcag cgaaccgtcg tcatcaccgg cgccaacagc gggttgggtg cggtgaccgc ccgcgagctg gcgcgacggg gggccaccgt catcatggcc gtgcgcgaca cccgcaaggg cgaggcggcc gcgcggacga tggccggcca ggtcgaggtc cgcgaactcg acctgcagga tctgtcgtcg gtgcgccggt tcgccgacgg ggtgagcggg gccgacgtgc tgatcaacaa tgccggcatc atggccgtcc cgtacgcgct gaccgtcgac ggcttcgaga gccagatcgg caccaaccac ctgggccatt tcgcgctgac caacctgctg ctgccccggc tcaccgaccg cgtcgtgacg gtgtcgtcga tggcgcactg gccgggacgg atcaacctcg aggatctgaa ctggcggtcg cgccggtact cgccgtggct ggcctacagc cagtccaagc tggccaacct gctgttcacc agcgagctgc agcgccgcct gacggccgcc ggttccccgc tgcgcgcgct ggccgcccac cccggctact cgcacaccaa cctgcagggc gcctcgggcc gcaagctggg cgacgcgctg atgtcggcgg cgacgcgggt ggtcgccacc gacgccgatt tcggcgcccg gcaaaccctg tacgcggcgt cccaggatct gcccggggac agcttcgtcg gcccccggtt cggttacctg ggccgcaccc aaccggtcgg acgcagccgg cgggccaaag atgccggcat ggcggcggcg ctgtgggcgc tctccgagca gctcaccaag accgaatttc cgctcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MypaA.01249.c.A1.PW29465
| PURIFICATION DATe: | 1/10/2011 |
| CONCENTRATION: | 28.66mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMTGWTAA DLPSFAQRTV VITGANSGLG AVTARELARR GATVIMAVRD TRKGEAAART MAGQVEVREL DLQDLSSVRR FADGVSGADV LINNAGIMAV PYALTVDGFE SQIGTNHLGH FALTNLLLPR LTDRVVTVSS MAHWPGRINL EDLNWRSRRY SPWLAYSQSK LANLLFTSEL QRRLTAAGSP LRALAAHPGY SHTNLQGASG RKLGDALMSA ATRVVATDAD FGARQTLYAA SQDLPGDSFV GPRFGYLGRT QPVGRSRRAK DAGMAAALWA LSEQLTKTEF PL
Validated NT Sequence
ttattagagc ggaaattcgg tcttggtgag ctgctcggag agcgcccaca gcgccgccgc catgccggca tctttggccc gccggctgcg tccgaccggt tgggtgcggc ccaggtaacc gaaccggggg ccgacgaagc tgtccccggg cagatcctgg gacgccgcgt acagggtttg ccgggcgccg aaatcggcgt cggtggcgac cacccgcgtc gccgccgaca tcagcgcgtc gcccagcttg cggcccgagg cgccctgcag gttggtgtgc gagtagccgg ggtgggcggc cagcgcgcgc agcggggaac cggcggccgt caggcggcgc tgcagctcgc tggtgaacag caggttggcc agcttggact ggctgtaggc cagccacggc gagtaccggc gcgaccgcca gttcagatcc tcgaggttga tccgtcccgg ccagtgcgcc atcgacgaca ccgtcacgac gcggtcggtg agccggggca gcagcaggtt ggtcagcgcg aaatggccca ggtggttggt gccgatctgg ctctcgaagc cgtcgacggt cagcgcgtac gggacggcca tgatgccggc attgttgatc agcacgtcgg ccccgctcac cccgtcggcg aaccggcgca ccgacgacag atcctgcagg tcgagttcgc ggacctcgac ctggccggcc atcgtccgcg cggccgcctc gcccttgcgg gtgtcgcgca cggccatgat gacggtggcc ccccgtcgcg ccagctcgcg ggcggtcacc gcacccaacc cgctgttggc gccggtgatg acgacggttc gctgcgcgaa cgacggtagg tctgcggcgg tccagcctgt catcgaacca ggaccctggg tctgagcttc cagggtaccc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTGWTAADL PSFAQRTVVI TGANSGLGAV TARELARRGA TVIMAVRDTR KGEAAARTMA GQVEVRELDL QDLSSVRRFA DGVSGADVLI NNAGIMAVPY ALTVDGFESQ IGTNHLGHFA LTNLLLPRLT DRVVTVSSMA HWPGRINLED LNWRSRRYSP WLAYSQSKLA NLLFTSELQR RLTAAGSPLR ALAAHPGYSH TNLQGASGRK LGDALMSAAT RVVATDADFG ARQTLYAASQ DLPGDSFVGP RFGYLGRTQP VGRSRRAKDA GMAAALWALS EQLTKTEFPL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAGGCTGG ACCGCCGCAG ACCTACCGTC GTTCGCGCAG CGAACCGTCG TCATCACCGG CGCCAACAGC GGGTTGGGTG CGGTGACCGC CCGCGAGCTG GCGCGACGGG GGGCCACCGT CATCATGGCC GTGCGCGACA CCCGCAAGGG CGAGGCGGCC GCGCGGACGA TGGCCGGCCA GGTCGAGGTC CGCGAACTCG ACCTGCAGGA TCTGTCGTCG GTGCGCCGGT TCGCCGACGG GGTGAGCGGG GCCGACGTGC TGATCAACAA TGCCGGCATC ATGGCCGTCC CGTACGCGCT GACCGTCGAC GGCTTCGAGA GCCAGATCGG CACCAACCAC CTGGGCCATT TCGCGCTGAC CAACCTGCTG CTGCCCCGGC TCACCGACCG CGTCGTGACG GTGTCGTCGA TGGCGCACTG GCCGGGACGG ATCAACCTCG AGGATCTGAA CTGGCGGTCG CGCCGGTACT CGCCGTGGCT GGCCTACAGC CAGTCCAAGC TGGCCAACCT GCTGTTCACC AGCGAGCTGC AGCGCCGCCT GACGGCCGCC GGTTCCCCGC TGCGCGCGCT GGCCGCCCAC CCCGGCTACT CGCACACCAA CCTGCAGGGC GCCTCGGGCC GCAAGCTGGG CGACGCGCTG ATGTCGGCGG CGACGCGGGT GGTCGCCACC GACGCCGATT TCGGCGCCCG GCAAACCCTG TACGCGGCGT CCCAGGATCT GCCCGGGGAC AGCTTCGTCG GCCCCCGGTT CGGTTACCTG GGCCGCACCC AACCGGTCGG ACGCAGCCGG CGGGCCAAAG ATGCCGGCAT GGCGGCGGCG CTGTGGGCGC TCTCCGAGCA GCTCACCAAG ACCGAATTTC CGCTCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA