MypaA.01249.a

Putative uncharacterized protein

CENTER ID: MypaA.01249.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01249.a.A1.GE29565 Full length( MypaA.01249.a ) 1 312
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01249.a.A1.PW29463 Full length( MypaA.01249.a ) 1 312

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.4185
RefSeq: NP_962867.1
UniProt: Q73SY9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAPHDKQHRD WSEADVGDQS GRVVVITGAN TGIGYETAAV LAHRGAHVVL AVRDLEKGNA ALSRIVAASP NADVTLQQLD LASLASVRSA AEALRAAYPR IDLLINNAGV MWTPKQVTED GFELQFGTNH LGHFALTGLL LDHLLGVRDS RVVTVSSLGH RLRAAIHFDD LHWERRYDRV AAYGQSKLAN LLFTYELQRR LAAAPDAKTI AVAAHPGGSN TELARHLPGI FRPVQAVLGP VLFQSPAMGA LPTLRAATDP AVQGGQYYGP DGFLEQRGRP KLVESSAQSH DEQLQRRLWA VSEELTGVHF PV
NT Sequence
ATGGCTCCCC ACGACAAACA GCACCGCGAC TGGTCGGAGG CCGACGTTGG TGATCAGAGC GGCCGCGTCG TCGTCATCAC CGGCGCCAAC ACCGGCATCG GCTACGAGAC GGCCGCCGTG CTGGCCCACC GCGGCGCCCA CGTCGTGCTC GCGGTCCGCG ACCTGGAGAA GGGCAACGCG GCGCTGTCGC GGATCGTGGC CGCCAGCCCG AACGCCGACG TCACGCTGCA GCAGCTGGAC CTGGCCTCGC TGGCCTCGGT GCGTTCGGCC GCCGAGGCGC TGCGCGCGGC CTACCCGCGC ATCGACCTGC TGATCAACAA CGCCGGCGTG ATGTGGACGC CCAAGCAGGT CACCGAGGAC GGTTTCGAAT TGCAGTTCGG CACCAACCAT CTGGGGCACT TCGCGCTGAC CGGGTTGCTG CTCGATCACC TGCTGGGAGT GCGGGATTCG CGGGTGGTGA CGGTCAGCAG CCTCGGCCAC CGGCTGCGCG CGGCCATCCA CTTCGACGAC CTGCACTGGG AGCGCCGCTA CGACCGGGTC GCCGCCTACG GCCAGTCCAA GCTGGCCAAC CTGCTGTTCA CCTACGAGCT GCAGCGCCGG CTGGCGGCCG CCCCGGACGC GAAGACCATC GCGGTCGCCG CGCACCCCGG CGGCTCCAAC ACCGAGCTGG CCCGCCACCT GCCCGGCATC TTCCGGCCGG TGCAGGCGGT GCTGGGGCCG GTGCTGTTCC AGAGCCCGGC GATGGGCGCG CTGCCGACGC TGCGGGCCGC CACCGATCCG GCCGTGCAGG GCGGGCAGTA CTACGGCCCG GACGGCTTCC TCGAACAGCG CGGGCGCCCG AAGCTGGTCG AGTCCAGCGC CCAGTCGCAC GACGAGCAGC TGCAGCGCCG GCTGTGGGCC GTGTCCGAAG AACTCACCGG CGTCCACTTC CCGGTC
Details for MypaA.01249.a.A1.GE29565
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAPHDKQHR DWSEADVGDQ SGRVVVITGA NTGIGYETAA VLAHRGAHVV LAVRDLEKGN AALSRIVAAS PNADVTLQQL DLASLASVRS AAEALRAAYP RIDLLINNAG VMWTPKQVTE DGFELQFGTN HLGHFALTGL LLDHLLGVRD SRVVTVSSLG HRLRAAIHFD DLHWERRYDR VAAYGQSKLA NLLFTYELQR RLAAAPDAKT IAVAAHPGGS NTELARHLPG IFRPVQAVLG PVLFQSPAMG ALPTLRAATD PAVQGGQYYG PDGFLEQRGR PKLVESSAQS HDEQLQRRLW AVSEELTGVH FP
Validated NT Sequence
gaccgggaag tggacgccgg tgagttcttc ggacacggcc cacagccggc gctgcagctg ctcgtcgtgc gactgggcgc tggactcgac cagcttcggg cgcccgcgct gttcgaggaa gccgtccggg ccgtagtact gcccgccctg cacggccgga tcggtggcgg cccgcagcgt cggcagcgcg cccatcgccg ggctctggaa cagcaccggc cccagcaccg cctgcaccgg ccggaagatg ccgggcaggt ggcgggccag ctcggtgttg gagccgccgg ggtgcgcggc gaccgcgatg gtcttcgcgt ccggggcggc cgccagccgg cgctgcagct cgtaggtgaa cagcaggttg gccagcttgg actggccgta ggcggcgacc cggtcgtagc ggcgctccca gtgcaggtcg tcgaagtgga tggccgcgcg cagccggtgg ccgaggctgc tgaccgtcac cacccgcgaa tcccgcactc ccagcaggtg atcgagcagc aacccggtca gcgcgaagtg ccccagatgg ttggtgccga actgcaattc gaaaccgtcc tcggtgacct gcttgggcgt ccacatcacg ccggcgttgt tgatcagcag gtcgatgcgc gggtaggccg cgcgcagcgc ctcggcggcc gaacgcaccg aggccagcga ggccaggtcc agctgctgca gcgtgacgtc ggcgttcggg ctggcggcca cgatccgcga cagcgccgcg ttgcccttct ccaggtcgcg gaccgcgagc acgacgtggg cgccgcggtg ggccagcacg gcggccgtct cgtagccgat gccggtgttg gcgccggtga tgacgacgac gcggccgctc tgatcaccaa cgtcggcctc cgaccagtcg cggtgctgtt tgtcgtgggg agccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAPHDKQHR DWSEADVGDQ SGRVVVITGA NTGIGYETAA VLAHRGAHVV LAVRDLEKGN AALSRIVAAS PNADVTLQQL DLASLASVRS AAEALRAAYP RIDLLINNAG VMWTPKQVTE DGFELQFGTN HLGHFALTGL LLDHLLGVRD SRVVTVSSLG HRLRAAIHFD DLHWERRYDR VAAYGQSKLA NLLFTYELQR RLAAAPDAKT IAVAAHPGGS NTELARHLPG IFRPVQAVLG PVLFQSPAMG ALPTLRAATD PAVQGGQYYG PDGFLEQRGR PKLVESSAQS HDEQLQRRLW AVSEELTGVH FPV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctccccac gacaaacagc accgcgactg gtcggaggcc gacgttggtg atcagagcgg ccgcgtcgtc gtcatcaccg gcgccaacac cggcatcggc tacgagacgg ccgccgtgct ggcccaccgc ggcgcccacg tcgtgctcgc ggtccgcgac ctggagaagg gcaacgcggc gctgtcgcgg atcgtggccg ccagcccgaa cgccgacgtc acgctgcagc agctggacct ggcctcgctg gcctcggtgc gttcggccgc cgaggcgctg cgcgcggcct acccgcgcat cgacctgctg atcaacaacg ccggcgtgat gtggacgccc aagcaggtca ccgaggacgg tttcgaattg cagttcggca ccaaccatct ggggcacttc gcgctgaccg ggttgctgct cgatcacctg ctgggagtgc gggattcgcg ggtggtgacg gtcagcagcc tcggccaccg gctgcgcgcg gccatccact tcgacgacct gcactgggag cgccgctacg accgggtcgc cgcctacggc cagtccaagc tggccaacct gctgttcacc tacgagctgc agcgccggct ggcggccgcc ccggacgcga agaccatcgc ggtcgccgcg caccccggcg gctccaacac cgagctggcc cgccacctgc ccggcatctt ccggccggtg caggcggtgc tggggccggt gctgttccag agcccggcga tgggcgcgct gccgacgctg cgggccgcca ccgatccggc cgtgcagggc gggcagtact acggcccgga cggcttcctc gaacagcgcg ggcgcccgaa gctggtcgag tccagcgccc agtcgcacga cgagcagctg cagcgccggc tgtgggccgt gtccgaagaa ctcaccggcg tccacttccc ggtcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MypaA.01249.a.A1.PW29463
PURIFICATION DATe: 9/7/2010
CONCENTRATION: 24.01mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAPHDKQHR DWSEADVGDQ SGRVVVITGA NTGIGYETAA VLAHRGAHVV LAVRDLEKGN AALSRIVAAS PNADVTLQQL DLASLASVRS AAEALRAAYP RIDLLINNAG VMWTPKQVTE DGFELQFGTN HLGHFALTGL LLDHLLGVRD SRVVTVSSLG HRLRAAIHFD DLHWERRYDR VAAYGQSKLA NLLFTYELQR RLAAAPDAKT IAVAAHPGGS NTELARHLPG IFRPVQAVLG PVLFQSPAMG ALPTLRAATD PAVQGGQYYG PDGFLEQRGR PKLVESSAQS HDEQLQRRLW AVSEELTGVH FP
Validated NT Sequence
gaccgggaag tggacgccgg tgagttcttc ggacacggcc cacagccggc gctgcagctg ctcgtcgtgc gactgggcgc tggactcgac cagcttcggg cgcccgcgct gttcgaggaa gccgtccggg ccgtagtact gcccgccctg cacggccgga tcggtggcgg cccgcagcgt cggcagcgcg cccatcgccg ggctctggaa cagcaccggc cccagcaccg cctgcaccgg ccggaagatg ccgggcaggt ggcgggccag ctcggtgttg gagccgccgg ggtgcgcggc gaccgcgatg gtcttcgcgt ccggggcggc cgccagccgg cgctgcagct cgtaggtgaa cagcaggttg gccagcttgg actggccgta ggcggcgacc cggtcgtagc ggcgctccca gtgcaggtcg tcgaagtgga tggccgcgcg cagccggtgg ccgaggctgc tgaccgtcac cacccgcgaa tcccgcactc ccagcaggtg atcgagcagc aacccggtca gcgcgaagtg ccccagatgg ttggtgccga actgcaattc gaaaccgtcc tcggtgacct gcttgggcgt ccacatcacg ccggcgttgt tgatcagcag gtcgatgcgc gggtaggccg cgcgcagcgc ctcggcggcc gaacgcaccg aggccagcga ggccaggtcc agctgctgca gcgtgacgtc ggcgttcggg ctggcggcca cgatccgcga cagcgccgcg ttgcccttct ccaggtcgcg gaccgcgagc acgacgtggg cgccgcggtg ggccagcacg gcggccgtct cgtagccgat gccggtgttg gcgccggtga tgacgacgac gcggccgctc tgatcaccaa cgtcggcctc cgaccagtcg cggtgctgtt tgtcgtgggg agccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAPHDKQHR DWSEADVGDQ SGRVVVITGA NTGIGYETAA VLAHRGAHVV LAVRDLEKGN AALSRIVAAS PNADVTLQQL DLASLASVRS AAEALRAAYP RIDLLINNAG VMWTPKQVTE DGFELQFGTN HLGHFALTGL LLDHLLGVRD SRVVTVSSLG HRLRAAIHFD DLHWERRYDR VAAYGQSKLA NLLFTYELQR RLAAAPDAKT IAVAAHPGGS NTELARHLPG IFRPVQAVLG PVLFQSPAMG ALPTLRAATD PAVQGGQYYG PDGFLEQRGR PKLVESSAQS HDEQLQRRLW AVSEELTGVH FPV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTCCCCAC GACAAACAGC ACCGCGACTG GTCGGAGGCC GACGTTGGTG ATCAGAGCGG CCGCGTCGTC GTCATCACCG GCGCCAACAC CGGCATCGGC TACGAGACGG CCGCCGTGCT GGCCCACCGC GGCGCCCACG TCGTGCTCGC GGTCCGCGAC CTGGAGAAGG GCAACGCGGC GCTGTCGCGG ATCGTGGCCG CCAGCCCGAA CGCCGACGTC ACGCTGCAGC AGCTGGACCT GGCCTCGCTG GCCTCGGTGC GTTCGGCCGC CGAGGCGCTG CGCGCGGCCT ACCCGCGCAT CGACCTGCTG ATCAACAACG CCGGCGTGAT GTGGACGCCC AAGCAGGTCA CCGAGGACGG TTTCGAATTG CAGTTCGGCA CCAACCATCT GGGGCACTTC GCGCTGACCG GGTTGCTGCT CGATCACCTG CTGGGAGTGC GGGATTCGCG GGTGGTGACG GTCAGCAGCC TCGGCCACCG GCTGCGCGCG GCCATCCACT TCGACGACCT GCACTGGGAG CGCCGCTACG ACCGGGTCGC CGCCTACGGC CAGTCCAAGC TGGCCAACCT GCTGTTCACC TACGAGCTGC AGCGCCGGCT GGCGGCCGCC CCGGACGCGA AGACCATCGC GGTCGCCGCG CACCCCGGCG GCTCCAACAC CGAGCTGGCC CGCCACCTGC CCGGCATCTT CCGGCCGGTG CAGGCGGTGC TGGGGCCGGT GCTGTTCCAG AGCCCGGCGA TGGGCGCGCT GCCGACGCTG CGGGCCGCCA CCGATCCGGC CGTGCAGGGC GGGCAGTACT ACGGCCCGGA CGGCTTCCTC GAACAGCGCG GGCGCCCGAA GCTGGTCGAG TCCAGCGCCC AGTCGCACGA CGAGCAGCTG CAGCGCCGGC TGTGGGCCGT GTCCGAAGAA CTCACCGGCG TCCACTTCCC GGTCAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA