MypaA.01189.b

FadB4

CENTER ID: MypaA.01189.b
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01189.b.A1.GE29559 Full length( MypaA.01189.b ) 1 323
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.3385
RefSeq: NP_962124.1
UniProt: Q73V26

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRAARVTRLD GPDAIEVTDV DEPTGDGVFV DVHAAGVAFP DALLTRGLYQ YRPELPFVLG AEIAGVVRSA PEGAHVRSGD RVVGLTMLSG GMAEVAVLAP DRVFKLPDKV SFEAGAGLLF NDLTVYFALT VRGRLQRGET VLVHGAAGGI GTSALRLAPA LGAARTIAVV STTDKADIAT AAGATDVVLA DGFKDAVKEL THGRGVDIVV DPVGGDRFTD SLRSLAPGGR LLVVGFTGGE IPTVKVNRLL LNNIDVVGVG WGAWTGTHPG ALAEQWAGLE RLLTSGELAP PEPVVYPLDE AAAAVASLEN RTAKGKVVLR VRG
NT Sequence
ATGCGTGCTG CACGAGTGAC TCGACTGGAC GGTCCGGACG CGATCGAGGT GACGGACGTC GACGAACCGA CCGGCGACGG CGTCTTCGTC GACGTGCACG CGGCCGGGGT GGCCTTCCCG GACGCCCTGC TCACCCGCGG CCTCTACCAG TACCGGCCGG AGTTGCCGTT CGTGCTCGGC GCCGAGATCG CCGGCGTGGT GCGGTCCGCG CCCGAGGGCG CGCACGTGCG TTCCGGCGAC CGGGTCGTCG GCCTCACCAT GCTGTCCGGC GGCATGGCCG AGGTGGCGGT GCTGGCCCCC GACCGGGTGT TCAAGCTGCC CGACAAGGTC AGCTTCGAGG CCGGCGCCGG CCTGCTGTTC AACGACCTGA CGGTGTACTT CGCGCTGACC GTGCGGGGCC GGCTGCAGCG CGGCGAGACG GTGCTGGTGC ACGGCGCGGC CGGCGGTATC GGCACCTCGG CGCTGCGACT GGCGCCGGCG CTGGGCGCCG CCCGCACCAT CGCCGTGGTC AGCACGACGG ACAAGGCCGA CATCGCCACG GCGGCCGGCG CGACGGACGT CGTGCTGGCC GACGGCTTCA AGGACGCCGT GAAGGAGCTG ACCCACGGCC GCGGCGTCGA CATCGTCGTC GACCCGGTCG GCGGCGACCG GTTCACCGAT TCGCTGCGCT CGCTGGCCCC CGGCGGGCGG CTGCTGGTGG TCGGCTTCAC CGGCGGCGAG ATCCCCACGG TCAAGGTAAA CCGGTTGCTA CTCAACAACA TCGACGTGGT CGGCGTCGGC TGGGGCGCCT GGACGGGCAC CCATCCCGGC GCGCTGGCCG AGCAGTGGGC GGGGCTCGAG CGACTGCTCA CCTCCGGTGA GCTGGCCCCG CCCGAGCCGG TGGTCTATCC GCTCGACGAG GCCGCGGCGG CCGTGGCCTC GCTGGAGAAC CGCACCGCCA AGGGCAAGGT CGTGTTGCGC GTGCGCGGT
Details for MypaA.01189.b.A1.GE29559
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 106
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRAARVTRL DGPDAIEVTD VDEPTGDGVF VDVHAAGVAF PDALLTRGLY QYRPELPFVL GAEIAGVVRS APEGAHVRSG DRVVGLTMLS GGMAEVAVLA PDRVFKLPDK VSFEAGAGLL FNDLTVYFAL TVRGRLQRGE TVLVHGAAGG IGTSALRLAP ALGAARTIAV VSTTDKADIA TAAGATDVVL ADGFKDAVKE LTHGRGVDIV VDPVGGDRFT DSLRSLAPGG RLLVVGFTGG EIPTVKVNRL LLNNIDVVGV GWGAWTGTHP GALAXQWAGL ERLLTSXSWP XPSXXXXRST XXXXXXXSLX XXXXGXVXXX XXXNXXXRXX XXSXXXXXXX XXXXXX
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgtg ctgcacgagt gactcgactg gacggtccgg acgcgatcga ggtgacggac gtcgacgaac cgaccggcga cggcgtcttc gtcgacgtgc acgcggccgg ggtggccttc ccggacgccc tgctcacccg cggcctctac cagtaccggc cggagttgcc gttcgtgctc ggcgccgaga tcgccggcgt ggtgcggtcc gcgcccgagg gcgcgcacgt gcgttccggc gaccgggtcg tcggcctcac catgctgtcc ggcggcatgg ccgaggtggc ggtgctggcc cccgaccggg tgttcaagct gcccgacaag gtcagcttcg aggccggcgc cggcctgctg ttcaacgacc tgacggtgta cttcgcgctg accgtgcggg gccggctgca gcgcggcgag acggtgctgg tgcacggcgc ggccggcggt atcggcacct cggcgctgcg actggcgccg gcgctgggcg ccgcccgcac catcgccgtg gtcagcacga cggacaaggc cgacatcgcc acggcggccg gcgcgacgga cgtcgtgctg gccgacggct tcaaggacgc cgtgaaggag ctgacccacg gccgcggcgt cgacatcgtc gtcgacccgg tcggcggcga ccggttcacc gattcgctgc gctcgctggc ccccggcggg cggctgctgg tggtcggctt caccggcggc gagatcccca cggtcaaggt aaaccggttg ctactcaaca acatcgacgt ggtcggcgtc ggctggggcg cctggacggg cacccatccc ggcgcgctgg ccgancagtg ggcggggctc gagcgactgc tcacctcnnn nagctggccc cnnccgagcn ggnggnctat ncgctcgacg anncngcngn ggnccgnnnn ntcnctgnan nancnnnnnc ngggnnnggt cnnnnnncnn nnnnnnnnna atnaannngn ncgaannnca nnnnnttcnc nnngnnnnnn nnnntancna nncnnaannn nnntnntng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRAARVTRL DGPDAIEVTD VDEPTGDGVF VDVHAAGVAF PDALLTRGLY QYRPELPFVL GAEIAGVVRS APEGAHVRSG DRVVGLTMLS GGMAEVAVLA PDRVFKLPDK VSFEAGAGLL FNDLTVYFAL TVRGRLQRGE TVLVHGAAGG IGTSALRLAP ALGAARTIAV VSTTDKADIA TAAGATDVVL ADGFKDAVKE LTHGRGVDIV VDPVGGDRFT DSLRSLAPGG RLLVVGFTGG EIPTVKVNRL LLNNIDVVGV GWGAWTGTHP GALAEQWAGL ERLLTSGELA PPEPVVYPLD EAAAAVASLE NRTAKGKVVL RVRG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtgctgca cgagtgactc gactggacgg tccggacgcg atcgaggtga cggacgtcga cgaaccgacc ggcgacggcg tcttcgtcga cgtgcacgcg gccggggtgg ccttcccgga cgccctgctc acccgcggcc tctaccagta ccggccggag ttgccgttcg tgctcggcgc cgagatcgcc ggcgtggtgc ggtccgcgcc cgagggcgcg cacgtgcgtt ccggcgaccg ggtcgtcggc ctcaccatgc tgtccggcgg catggccgag gtggcggtgc tggcccccga ccgggtgttc aagctgcccg acaaggtcag cttcgaggcc ggcgccggcc tgctgttcaa cgacctgacg gtgtacttcg cgctgaccgt gcggggccgg ctgcagcgcg gcgagacggt gctggtgcac ggcgcggccg gcggtatcgg cacctcggcg ctgcgactgg cgccggcgct gggcgccgcc cgcaccatcg ccgtggtcag cacgacggac aaggccgaca tcgccacggc ggccggcgcg acggacgtcg tgctggccga cggcttcaag gacgccgtga aggagctgac ccacggccgc ggcgtcgaca tcgtcgtcga cccggtcggc ggcgaccggt tcaccgattc gctgcgctcg ctggcccccg gcgggcggct gctggtggtc ggcttcaccg gcggcgagat ccccacggtc aaggtaaacc ggttgctact caacaacatc gacgtggtcg gcgtcggctg gggcgcctgg acgggcaccc atcccggcgc gctggccgag cagtgggcgg ggctcgagcg actgctcacc tccggtgagc tggccccgcc cgagccggtg gtctatccgc tcgacgaggc cgcggcggcc gtggcctcgc tggagaaccg caccgccaag ggcaaggtcg tgttgcgcgt gcgcggtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa