MypaA.01124.a

DrrA

CENTER ID: MypaA.01124.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01124.a.A1.GE29551 Full length( MypaA.01124.a ) 1 315
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.1301
RefSeq: NP_960172.1
UniProt: Q740V4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAPAIEVEG LTKKFGGSTL ALAGVDFSVP AGTVCGLLGH NGAGKTTTIN ILSTLIRPTS GRASVAGHDV VRRPAEVRAS IGMAGQFTGL DPMLTGRENL VLFGRLRGLN RRRAKARADE LLEQFNLVDA ADRRLSTYSG GMFRRVDLAS ALVVPPKVLF LDEPTTGLDP RSRRDVWALV SSLRVQGITV LLTTQYLEEA DVLSDSIVVI DHGRVIASGT SEELKRAVGT SYCQVTPANP ADLPQVVATL TGLDGVDIDN DTGSVSVFAP DGVATLVEVF RRVDALGLEL ADISLRKPSL DEAFLHLTER TVARP
NT Sequence
GTGAGCGCAC CGGCGATCGA GGTCGAAGGC CTCACAAAGA AATTCGGGGG AAGCACGCTC GCACTGGCCG GGGTGGACTT CTCGGTTCCG GCCGGGACGG TGTGCGGGCT GCTCGGCCAC AACGGCGCCG GCAAAACCAC CACGATCAAC ATCTTGTCCA CGTTGATCCG CCCGACGTCG GGCCGGGCCA GTGTCGCCGG GCATGACGTC GTACGCCGCC CCGCCGAGGT ACGTGCCAGC ATCGGGATGG CCGGACAGTT CACCGGACTG GACCCCATGC TGACCGGCCG GGAGAACCTG GTGCTGTTCG GCAGGCTGCG CGGACTCAAT CGGCGGCGGG CGAAGGCCCG CGCCGATGAA CTGCTCGAAC AGTTCAACCT GGTCGACGCG GCCGATCGGC GGCTGTCCAC CTATTCCGGG GGCATGTTCC GCCGCGTCGA TTTGGCCAGC GCCCTGGTGG TGCCGCCCAA GGTGCTCTTC CTCGACGAGC CGACCACCGG GCTGGACCCG CGCAGCCGCC GCGACGTGTG GGCGCTGGTG AGTTCGCTGC GGGTGCAGGG CATTACCGTG CTGCTGACCA CCCAGTACCT GGAAGAGGCC GACGTGCTGA GCGACTCGAT CGTCGTCATC GATCACGGGC GGGTGATCGC CAGCGGCACG TCCGAGGAAC TCAAGCGGGC GGTGGGGACC AGCTACTGCC AGGTGACCCC GGCCAACCCC GCCGACCTGC CCCAGGTAGT GGCCACGCTG ACCGGCCTTG ACGGGGTTGA TATCGACAAC GACACCGGTT CGGTATCGGT GTTCGCCCCG GACGGCGTGG CGACCCTGGT CGAGGTATTC CGCCGCGTGG ACGCGCTGGG GCTCGAGCTC GCCGACATCT CGCTGCGCAA GCCCTCGCTG GACGAGGCGT TCCTGCACCT GACCGAACGG ACCGTCGCGC GACCA
Details for MypaA.01124.a.A1.GE29551
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MVSAPAIEVE GLTKKFGGST LALAGVDFSV PAGTVCGLLG HNGAGKTTTI NILSTLIRPT SGRASVAGHD VVRRPAEVRA SIGMAGQFTG LDPMLTGREN LVLFGRLRGL NRRRAKARAD ELLEQFNLVD AADRRLSTYS GGMFRRVDLA SALVVPPKVL FLDEPTTGLD PRSRRDVWAL VSSLRVQGIT VLLTTQYLEE ADVLSDSIVV IDHGRVIASG TSEELKRAVG TSYCQVTPAN PADLPQVVAT LTGLDGVDID NDTGSVSVFA PDGVATLVEV FRRVDALGLE LADISLRKPS LDEAFLHLTE RTVARP
Validated NT Sequence
tattatggtc gcgcgacggt ccgttcggtc aggtgcagga acgcctcgtc cagcgagggc ttgcgcagcg agatgtcggc gagctcgagc cccagcgcgt ccacgcggcg gaatacctcg accagggtcg ccacgccgtc cggggcgaac accgataccg aaccggtgtc gttgtcgata tcaaccccgt caaggccggt cagcgtggcc actacctggg gcaggtcggc ggggttggcc ggggtcacct ggcagtagct ggtccccacc gcccgcttga gttcctcgga cgtgccgctg gcgatcaccc gcccgtgatc gatgacgacg atcgagtcgc tcagcacgtc ggcctcttcc aggtactggg tggtcagcag cacggtaatg ccctgcaccc gcagcgaact caccagcgcc cacacgtcgc ggcggctgcg cgggtccagc ccggtggtcg gctcgtcgag gaagagcacc ttgggcggca ccaccagggc gctggccaaa tcgacgcggc ggaacatgcc cccggaatag gtggacagcc gccgatcggc cgcgtcgacc aggttgaact gttcgagcag ttcatcggcg cgggccttcg cccgccgccg attgagtccg cgcagcctgc cgaacagcac caggttctcc cggccggtca gcatggggtc cagtccggtg aactgtccgg ccatcccgat gctggcacgt acctcggcgg ggcggcgtac gacgtcatgc ccggcgacac tggcccggcc cgacgtcggg cggatcaacg tggacaagat gttgatcgtg gtggttttgc cggcgccgtt gtggccgagc agcccgcaca ccgtcccggc cggaaccgag aagtccaccc cggccagtgc gagcgtgctt cccccgaatt tctttgtgag gccttcgacc tcgatcgccg gtgcgctcac catcgaacca ggaccctggg tctgagcttc caggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSAPAIEV EGLTKKFGGS TLALAGVDFS VPAGTVCGLL GHNGAGKTTT INILSTLIRP TSGRASVAGH DVVRRPAEVR ASIGMAGQFT GLDPMLTGRE NLVLFGRLRG LNRRRAKARA DELLEQFNLV DAADRRLSTY SGGMFRRVDL ASALVVPPKV LFLDEPTTGL DPRSRRDVWA LVSSLRVQGI TVLLTTQYLE EADVLSDSIV VIDHGRVIAS GTSEELKRAV GTSYCQVTPA NPADLPQVVA TLTGLDGVDI DNDTGSVSVF APDGVATLVE VFRRVDALGL ELADISLRKP SLDEAFLHLT ERTVARP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgca ccggcgatcg aggtcgaagg cctcacaaag aaattcgggg gaagcacgct cgcactggcc ggggtggact tctcggttcc ggccgggacg gtgtgcgggc tgctcggcca caacggcgcc ggcaaaacca ccacgatcaa catcttgtcc acgttgatcc gcccgacgtc gggccgggcc agtgtcgccg ggcatgacgt cgtacgccgc cccgccgagg tacgtgccag catcgggatg gccggacagt tcaccggact ggaccccatg ctgaccggcc gggagaacct ggtgctgttc ggcaggctgc gcggactcaa tcggcggcgg gcgaaggccc gcgccgatga actgctcgaa cagttcaacc tggtcgacgc ggccgatcgg cggctgtcca cctattccgg gggcatgttc cgccgcgtcg atttggccag cgccctggtg gtgccgccca aggtgctctt cctcgacgag ccgaccaccg ggctggaccc gcgcagccgc cgcgacgtgt gggcgctggt gagttcgctg cgggtgcagg gcattaccgt gctgctgacc acccagtacc tggaagaggc cgacgtgctg agcgactcga tcgtcgtcat cgatcacggg cgggtgatcg ccagcggcac gtccgaggaa ctcaagcggg cggtggggac cagctactgc caggtgaccc cggccaaccc cgccgacctg ccccaggtag tggccacgct gaccggcctt gacggggttg atatcgacaa cgacaccggt tcggtatcgg tgttcgcccc ggacggcgtg gcgaccctgg tcgaggtatt ccgccgcgtg gacgcgctgg ggctcgagct cgccgacatc tcgctgcgca agccctcgct ggacgaggcg ttcctgcacc tgaccgaacg gaccgtcgcg cgaccaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa