MypaA.01117.d

FadE26_1

CENTER ID: MypaA.01117.d
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01117.d.A1.GE29549 Full length( MypaA.01117.d ) 1 392
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.592
RefSeq: NP_959493.1
UniProt: Q743N2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRISYTPEQE DLRRELRSYF TKLMTPERRE ALSSTQGEYG VGNVYRETVA QMGKDGWLTL NWPKEYGGQD RSPMDSLIFT DEAAIAGAPV PFLTINSVAP TIMAFGTDEQ KKYFLPKIAA GELHFSIGYS EPGAGTDLAN LRTTAVRDGD DYVINGQKMW TSLIAYADWV WLAVRTNPEA KKHRGISMLV VPTTAEGFSW TPVHTMAGPD TSATYYSDVR VPVTNLVGEE NGGWKLVTNQ LNHERVALVS PAPIYLALRE VREWAQNTKD ASGARLIDSE WVQINLARVH AKAEVLKLIN WELASSDGHA LGPADASAAK VYGTELATEA YRLLMEVLGT AATVRQDSPG ALLRGRVERM HRACLILTFG GGTNEVQRDI IGMVALGLPR NR
NT Sequence
ATGCGCATCA GTTATACCCC GGAGCAGGAG GACCTGCGTC GCGAGCTGAG GTCGTACTTC ACCAAGCTGA TGACGCCCGA GCGCCGCGAG GCGCTGAGCT CGACGCAGGG CGAATACGGG GTCGGCAACG TCTATCGCGA AACCGTCGCC CAGATGGGCA AGGACGGCTG GCTGACCCTG AACTGGCCCA AGGAGTACGG CGGCCAGGAC CGCTCCCCGA TGGACTCGCT GATCTTCACC GACGAGGCCG CCATCGCCGG CGCGCCGGTG CCGTTCCTGA CGATCAACAG CGTGGCGCCG ACGATCATGG CGTTCGGCAC CGACGAGCAG AAGAAGTACT TCCTGCCCAA GATCGCCGCG GGCGAGCTGC ACTTCTCCAT CGGCTACTCC GAACCGGGCG CCGGCACCGA CCTGGCCAAC CTGCGCACCA CCGCGGTGCG CGACGGCGAC GACTATGTGA TCAACGGCCA GAAGATGTGG ACCAGCCTGA TCGCCTACGC CGACTGGGTG TGGCTGGCGG TGCGCACCAA CCCCGAGGCC AAGAAGCACC GCGGCATCTC GATGCTGGTG GTGCCCACCA CCGCGGAGGG TTTCTCCTGG ACGCCGGTGC ACACCATGGC CGGCCCCGAC ACCAGCGCCA CCTATTACTC CGACGTGCGG GTGCCGGTGA CCAACCTGGT CGGCGAGGAG AACGGCGGCT GGAAGCTGGT GACCAACCAG CTCAACCACG AGCGCGTCGC GCTGGTCTCG CCGGCGCCGA TCTATTTGGC GCTGCGCGAG GTGCGGGAAT GGGCGCAGAA CACCAAGGAC GCCAGCGGCG CCCGGCTCAT CGACTCCGAG TGGGTGCAGA TCAACCTGGC CCGGGTGCAC GCCAAGGCCG AGGTGCTCAA GCTGATCAAC TGGGAGCTGG CCTCCTCCGA CGGCCACGCC CTGGGGCCGG CCGACGCCTC GGCGGCCAAG GTGTACGGCA CCGAGCTGGC CACCGAGGCC TACCGGCTGC TGATGGAGGT GCTGGGTACC GCGGCCACCG TCCGTCAGGA CTCGCCCGGC GCCCTGCTGC GCGGCCGGGT GGAGCGGATG CACCGGGCGT GTTTGATCCT GACCTTCGGC GGCGGCACCA ACGAGGTGCA GCGCGACATC ATCGGCATGG TCGCGCTGGG ACTGCCCCGC AACCGC
Details for MypaA.01117.d.A1.GE29549
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 77
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRISYTPEQ EDLRRELRSY FTKLMTPERR EALSSTQGEY GVGNVYRETV AQMGKDGWLT LNWPKEYGGQ DRSPMDSLIF TDEAAIAGAP VPFLTINSVA PTIMAFGTDE QKKYFLPKIA AGELHFSIGY SEPGAGTDLA NLRTTAVRDG DDYVINGQKM WTSLIAYADW VWLAVRTNPE AKKHRGISML VVPTTAXGFS WTPVHTMAGP DTSATYYSDV RVPVXNLVGX ENGGWKLVTN QLNHERVALV SPAPIYLALR EVREWAQNTX XRQRXPAHRL RXGXQIXPGP XXRXXXXXSS
Validated NT Sequence
ttttgtttac tttaagaagg anatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgcgcat cagttatacc ccggagcagg aggacctgcg tcgcgagctg aggtcgtact tcaccaagct gatgacgccc gagcgccgcg aggcgctgag ctcgacgcag ggcgaatacg gggtcggcaa cgtctatcgc gaaaccgtcg cccagatggg caaggacggc tggctgaccc tgaactggcc caaggagtac ggcggccagg accgctcccc gatggactcg ctgatcttca ccgacgaggc cgccatcgcc ggcgcgccgg tgccgttcct gacgatcaac agcgtggcgc cgacgatcat ggcgttcggc accgacgagc agaagaagta cttcctgccc aagatcgccg cgggcgagct gcacttctcc atcggctact ccgaaccggg cgccggcacc gacctggcca acctgcgcac caccgcggtg cgcgacggcg acgactatgt gatcaacggc cagaagatgt ggaccagcct gatcgcctac gccgactggg tgtggctggc ggtgcgcacc aaccccgagg ccaagaagca ccgcggcatc tcgatgctgg tggtgcccac caccgcggan ggtttctcct ggacgccggt gcacaccatg gccggncccg acaccagcgc cacctattac tccgacgtgc gggtgccggt gancaacctg gtcggcgang agaacggcgg ctggaagctg gtgaccaacc agctcaacca cgagcgcgtc gcgctggtct cgccggcgcc gatctatttg gcgctgcgcg aggtgcggga atgggcgcag aacaccaang nacgccagcg gngcccggct catcgactcc gnnntgggnt gcagatcnan cctggncccg nnncacgccn anggnnnang ngctcaagct gatcnactgg nnnntngnnn ncnnnnnnnn acnnncnggg nnnngnnnna cnnntngnnn nnnnnnnnna cgnnnncntn nnnnnnnnnn ntannnggnn ncnnannnnn nnnngnnnnc ncnnnnnnng nnnnnnnnnn nnnnnccnnn nnnnnnnnnn nnnnggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRISYTPEQ EDLRRELRSY FTKLMTPERR EALSSTQGEY GVGNVYRETV AQMGKDGWLT LNWPKEYGGQ DRSPMDSLIF TDEAAIAGAP VPFLTINSVA PTIMAFGTDE QKKYFLPKIA AGELHFSIGY SEPGAGTDLA NLRTTAVRDG DDYVINGQKM WTSLIAYADW VWLAVRTNPE AKKHRGISML VVPTTAEGFS WTPVHTMAGP DTSATYYSDV RVPVTNLVGE ENGGWKLVTN QLNHERVALV SPAPIYLALR EVREWAQNTK DASGARLIDS EWVQINLARV HAKAEVLKLI NWELASSDGH ALGPADASAA KVYGTELATE AYRLLMEVLG TAATVRQDSP GALLRGRVER MHRACLILTF GGGTNEVQRD IIGMVALGLP RNR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcatcagt tataccccgg agcaggagga cctgcgtcgc gagctgaggt cgtacttcac caagctgatg acgcccgagc gccgcgaggc gctgagctcg acgcagggcg aatacggggt cggcaacgtc tatcgcgaaa ccgtcgccca gatgggcaag gacggctggc tgaccctgaa ctggcccaag gagtacggcg gccaggaccg ctccccgatg gactcgctga tcttcaccga cgaggccgcc atcgccggcg cgccggtgcc gttcctgacg atcaacagcg tggcgccgac gatcatggcg ttcggcaccg acgagcagaa gaagtacttc ctgcccaaga tcgccgcggg cgagctgcac ttctccatcg gctactccga accgggcgcc ggcaccgacc tggccaacct gcgcaccacc gcggtgcgcg acggcgacga ctatgtgatc aacggccaga agatgtggac cagcctgatc gcctacgccg actgggtgtg gctggcggtg cgcaccaacc ccgaggccaa gaagcaccgc ggcatctcga tgctggtggt gcccaccacc gcggagggtt tctcctggac gccggtgcac accatggccg gccccgacac cagcgccacc tattactccg acgtgcgggt gccggtgacc aacctggtcg gcgaggagaa cggcggctgg aagctggtga ccaaccagct caaccacgag cgcgtcgcgc tggtctcgcc ggcgccgatc tatttggcgc tgcgcgaggt gcgggaatgg gcgcagaaca ccaaggacgc cagcggcgcc cggctcatcg actccgagtg ggtgcagatc aacctggccc gggtgcacgc caaggccgag gtgctcaagc tgatcaactg ggagctggcc tcctccgacg gccacgccct ggggccggcc gacgcctcgg cggccaaggt gtacggcacc gagctggcca ccgaggccta ccggctgctg atggaggtgc tgggtaccgc ggccaccgtc cgtcaggact cgcccggcgc cctgctgcgc ggccgggtgg agcggatgca ccgggcgtgt ttgatcctga ccttcggcgg cggcaccaac gaggtgcagc gcgacatcat cggcatggtc gcgctgggac tgccccgcaa ccgcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa