SSGCID: Target Page

MypaA.01117.b

Putative uncharacterized protein

CENTER ID:	MypaA.01117.b
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01117.b.A1.GE29547	Full length( MypaA.01117.b )		1	382

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.2501
RefSeq:	NP_961286.1
UniProt:	Q73XF7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDYDLGDDAA QLRSHLRELV ANHIPADFLG ACTENPQDLA TTETFCKLLA AEGLLALAWP KEHGGGGGSV WQQTVLREEM WAQHEPRGPQ YMGINWVGPA LMRYGTEEQK AKHLAAIAAG DVIWCQGFSE PEAGTDLVSL RTRAVADGDG WRITGQKVWT SYALMASWCV LAACTDPDAP KHKRLTLFLI PMDRPGFTVR PIPSMLGPHH LNEMFLDDVP AYPGDVLGEV GDGWRVMREA LAYERVGIAR YARCESLLER MRGRLGDEWE QLPESIRARW VRALVDLRVA RLLAYRAVSL QDDPAAGAAA SAARIATTTC DQQVAELLFD VLGPTALDAG SGAPLHGAIE DHWRYAQAAT VASGTIEVQK MLVARDVLGG HR

NT Sequence

ATGGACTACG ACCTCGGCGA CGACGCCGCC CAACTGCGAA GCCACCTGCG CGAGCTGGTC GCCAACCACA TTCCCGCCGA CTTCCTGGGC GCCTGCACGG AGAACCCGCA GGACCTCGCC ACCACCGAGA CGTTCTGCAA ACTGCTGGCC GCCGAGGGGC TGCTGGCCCT TGCGTGGCCG AAAGAGCATG GCGGCGGCGG TGGTTCGGTC TGGCAGCAGA CCGTGCTGCG CGAGGAGATG TGGGCCCAGC ACGAGCCGCG CGGCCCGCAG TACATGGGCA TCAACTGGGT CGGGCCGGCC CTGATGCGCT ACGGCACCGA GGAGCAGAAG GCCAAGCACC TGGCGGCCAT CGCCGCCGGC GACGTGATCT GGTGCCAGGG ATTCTCCGAG CCGGAGGCCG GCACCGATCT GGTGTCGTTG CGCACCCGCG CCGTTGCCGA CGGTGACGGT TGGCGGATCA CCGGCCAGAA GGTGTGGACG TCGTACGCGC TGATGGCATC GTGGTGCGTG CTGGCGGCCT GCACCGACCC CGACGCGCCA AAACACAAGC GGCTCACGCT CTTTCTGATA CCGATGGACC GGCCCGGGTT CACCGTCCGG CCGATCCCGT CCATGCTGGG GCCGCATCAC CTCAACGAGA TGTTCCTCGA CGACGTCCCG GCGTATCCCG GCGACGTACT GGGCGAGGTC GGCGACGGCT GGCGGGTGAT GCGCGAGGCG CTGGCCTACG AACGGGTGGG GATCGCGCGC TACGCGCGCT GCGAGTCACT GCTGGAGCGG ATGCGCGGCC GGCTCGGTGA CGAGTGGGAG CAACTGCCCG AATCGATCCG GGCCCGCTGG GTGCGCGCGC TCGTCGACCT GCGGGTCGCC CGGCTGCTGG CCTATCGCGC CGTGTCGCTG CAGGACGACC CGGCCGCCGG CGCAGCGGCC AGCGCCGCCC GCATCGCCAC CACCACGTGC GATCAGCAGG TCGCCGAGCT GTTGTTCGAC GTGCTGGGCC CGACCGCGCT GGATGCCGGG TCCGGCGCGC CGCTGCACGG GGCCATCGAA GATCATTGGC GTTACGCACA GGCAGCCACC GTCGCATCGG GCACCATCGA GGTGCAGAAG ATGTTGGTGG CCCGAGACGT ACTGGGAGGG CACCGG

Details for MypaA.01117.b.A1.GE29547

HARVESTED ON:	6/15/2010
SEQUENCED ON:	6/27/2010
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	71

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDYDLGDDA AQLRSHLREL VANHIPADFL GACTENPQDL ATTETFCKLL AAEGLLALAW PKEHGGGGGS VWQQTVLREE MWAQHEPRGP QYMGINWVGP ALMRYGTEEQ KAKHLAAIAA GDVIWCQGFS EPEAGTDLVS LRTRAVADGD GWRITGQKVW TSYALMASWC VLAACTDPDA PKHKRLTLFL IPMDRPGFTV RPIPSMLGPH HLNEMFLDDV PAYPGDVLGE VGDGWRVMRE ALAYERVGIA RYARCESLLE RXRGPAR

Validated NT Sequence

attttgnnnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggact acgacctcgg cgacgacgcc gcccaactgc gaagccacct gcgcgagctg gtcgccaacc acattcccgc cgacttcctg ggcgcctgca cggagaaccc gcaggacctc gccaccaccg agacgttctg caaactgctg gccgccgagg ggctgctggc ccttgcgtgg ccgaaagagc atggcggcgg cggtggttcg gtctggcagc agaccgtgct gcgcgaggag atgtgggccc agcacgagcc gcgcggcccg cagtacatgg gcatcaactg ggtcgggccg gccctgatgc gctacggcac cgaggagcag aaggccaagc acctggcggc catcgccgcc ggcgacgtga tctggtgcca gggattctcc gagccggagg ccggcaccga tctggtgtcg ttgcgcaccc gcgccgttgc cgacggtgac ggttggcgga tcaccggcca gaaggtgtgg acgtcgtacg cgctgatggc atcgtggtgc gtgctggcgg cctgcaccga ccccgacgcg ccaaaacaca agcggctcac gctctttctg ataccgatgg accggcccgg gttcaccgtc cggccgatcc cgtccatgct ggggccgcat cacctcaacg agatgttcct cgacgacgtc ccggcgtatc ccggcgacgt actgggcgag gtcggcgacg gctggcgggt gatgcgcgag gcgctggcct acgaacgggt ggggatcgcg cgctacgcgc gctgcgagtc actgctggag cggntgcgcg gnccggctcg gtgacgagtg ggancaactg cccnaatcga tcccgggncc gctgggtgcn cgcgctcgtc nannngcggg nnnnccnggc tgctggnnta tcncgccnng ncgctgcnnn cganccngnn nncggngnnn cnnnnnccgn nntcnncncn ccnnnnncna tcannnnncn nnantnnnna nnnnnntgnn ccnannnnnn tgnnnncnnn ncngnnnnnn ncnnnnnngn nnntcnnnan nnnnnnnnnn nnnnnnnggn nnncncnncn nnnncnnnnn nnnnnnnnnn nnaana

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggactacgac ctcggcgacg acgccgccca actgcgaagc cacctgcgcg agctggtcgc caaccacatt cccgccgact tcctgggcgc ctgcacggag aacccgcagg acctcgccac caccgagacg ttctgcaaac tgctggccgc cgaggggctg ctggcccttg cgtggccgaa agagcatggc ggcggcggtg gttcggtctg gcagcagacc gtgctgcgcg aggagatgtg ggcccagcac gagccgcgcg gcccgcagta catgggcatc aactgggtcg ggccggccct gatgcgctac ggcaccgagg agcagaaggc caagcacctg gcggccatcg ccgccggcga cgtgatctgg tgccagggat tctccgagcc ggaggccggc accgatctgg tgtcgttgcg cacccgcgcc gttgccgacg gtgacggttg gcggatcacc ggccagaagg tgtggacgtc gtacgcgctg atggcatcgt ggtgcgtgct ggcggcctgc accgaccccg acgcgccaaa acacaagcgg ctcacgctct ttctgatacc gatggaccgg cccgggttca ccgtccggcc gatcccgtcc atgctggggc cgcatcacct caacgagatg ttcctcgacg acgtcccggc gtatcccggc gacgtactgg gcgaggtcgg cgacggctgg cgggtgatgc gcgaggcgct ggcctacgaa cgggtgggga tcgcgcgcta cgcgcgctgc gagtcactgc tggagcggat gcgcggccgg ctcggtgacg agtgggagca actgcccgaa tcgatccggg cccgctgggt gcgcgcgctc gtcgacctgc gggtcgcccg gctgctggcc tatcgcgccg tgtcgctgca ggacgacccg gccgccggcg cagcggccag cgccgcccgc atcgccacca ccacgtgcga tcagcaggtc gccgagctgt tgttcgacgt gctgggcccg accgcgctgg atgccgggtc cggcgcgccg ctgcacgggg ccatcgaaga tcattggcgt tacgcacagg cagccaccgt cgcatcgggc accatcgagg tgcagaagat gttggtggcc cgagacgtac tgggagggca ccggaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa