MypaA.01097.e

Putative uncharacterized protein

CENTER ID: MypaA.01097.e
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01097.e.A2.GE29541 Mature protein( MypaA.01097.e ) 23 274
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.2693
RefSeq: NP_961471.1
UniProt: Q73WX3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQGFAGKVAV VTGAGSGIGQ ALAVELARSG AKVAISDVDL EGLAHTEEQL KAIGAQYKAD RLDVTEREAF LAYADAVKEH FGKVNQIYNN AGIAFTGDVE VSQFKDIERV MDVDFWGVVN GTKAFLPHLI ASGDGHVVNV SSVFGLFSVP GQAAYNSAKF AVRGFTEALR QEMAAAGHPV AVTTVHPGGI KTAIARNATA AEGLDQAELA KLFDKRLAKT TPQRAAQIIL DAVRKKKARV LVGSDAKALD ILVRLTGSGY QRLFGPVMSR LLPN
NT Sequence
ATGCAGGGGT TCGCCGGCAA GGTCGCCGTG GTCACCGGCG CGGGATCGGG AATCGGGCAG GCGCTGGCCG TCGAGCTGGC CCGCTCGGGC GCCAAGGTGG CGATCAGCGA CGTCGACCTC GAAGGCCTGG CCCACACCGA GGAACAGCTG AAGGCGATCG GCGCGCAGTA CAAGGCCGAT CGGCTCGACG TGACCGAGCG CGAGGCGTTC CTGGCCTACG CCGACGCGGT CAAGGAGCAC TTCGGCAAGG TCAACCAGAT CTACAACAAC GCCGGCATCG CCTTCACCGG CGACGTCGAG GTCAGCCAGT TCAAGGACAT CGAACGGGTG ATGGACGTCG ACTTCTGGGG CGTGGTCAAC GGCACAAAGG CGTTCCTGCC GCACCTGATC GCCTCCGGCG ACGGACACGT CGTCAACGTC TCCAGCGTGT TCGGCCTGTT CTCGGTGCCG GGCCAGGCGG CCTACAACTC GGCCAAGTTC GCCGTGCGCG GCTTCACCGA GGCGCTGCGC CAGGAGATGG CCGCGGCCGG TCACCCGGTG GCGGTGACGA CCGTGCACCC CGGCGGCATC AAGACCGCGA TCGCCCGCAA CGCCACCGCG GCCGAGGGCC TGGACCAGGC CGAGCTGGCC AAGCTGTTCG ACAAGCGGCT GGCCAAGACC ACGCCGCAGC GCGCCGCCCA GATCATCCTG GACGCGGTGC GCAAGAAGAA GGCGCGGGTG CTGGTCGGCT CGGACGCCAA GGCGCTCGAC ATCCTGGTGC GGCTGACCGG TTCGGGCTAC CAGCGGCTGT TCGGGCCGGT CATGAGCCGG CTGCTGCCCA AC
Details for MypaA.01097.e.A2.GE29541
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAVELARSG AKVAISDVDL EGLAHTEEQL KAIGAQYKAD RLDVTEREAF LAYADAVKEH FGKVNQIYNN AGIAFTGDVE VSQFKDIERV MDVDFWGVVN GTKAFLPHLI ASGDGHVVNV SSVFGLFSVP GQAAYNSAKF AVRGFTEALR QEMAAAGHPV AVTTVHPGGI KTAIARNATA AXGLDQAELA KLFDKRLAKT TPQRAAQIIL DAVRKKKARV LVGSDAKALD ILVRLTGSGY QRLFGPVMSR LLPN
Validated NT Sequence
attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggcc gtcgagctgg cccgctcggg cgccaaggtg gcgatcagcg acgtcgacct cgaaggcctg gcccacaccg aggaacagct gaaggcgatc ggcgcgcagt acaaggccga tcggctcgac gtgaccgagc gcgaggcgtt cctggcctac gccgacgcgg tcaaggagca cttcggcaag gtcaaccaga tctacaacaa cgccggcatc gccttcaccg gcgacgtcga ggtcagccag ttcaaggaca tcgaacgggt gatggacgtc gacttctggg gcgtggtcaa cggcacaaag gcgttcctgc cgcacctgat cgcctccggc gacggacacg tcgtcaacgt ctccagcgtg ttcggcctgt tctcggtgcc gggccaggcg gcctacaact cggccaagtt cgccgtgcgc ggcttcaccg aggcgctgcg ccaggagatg gccgcggccg gtcacccggt ggcggtgacg accgtgcacc ccggcggcat caagaccgcg atcgcccgca acgccaccgc ggccganggc ctggaccagg ccgagctggc caagctgttc gacaagcggc tggccaagac cacgccgcag cgcgccgccc agatcatcct ggacgcggtg cgcaagaaga aggcgcgggt gctggtcggc tcggacgcca aggcgctcga catcctggtg cggctgaccg gttcgggcta ccagcggctg ttcgggccgg tcatgagccg gctgctgccc aactaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggnanctga gttggctgct gcnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAVELARSG AKVAISDVDL EGLAHTEEQL KAIGAQYKAD RLDVTEREAF LAYADAVKEH FGKVNQIYNN AGIAFTGDVE VSQFKDIERV MDVDFWGVVN GTKAFLPHLI ASGDGHVVNV SSVFGLFSVP GQAAYNSAKF AVRGFTEALR QEMAAAGHPV AVTTVHPGGI KTAIARNATA AEGLDQAELA KLFDKRLAKT TPQRAAQIIL DAVRKKKARV LVGSDAKALD ILVRLTGSGY QRLFGPVMSR LLPN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgtcgag ctggcccgct cgggcgccaa ggtggcgatc agcgacgtcg acctcgaagg cctggcccac accgaggaac agctgaaggc gatcggcgcg cagtacaagg ccgatcggct cgacgtgacc gagcgcgagg cgttcctggc ctacgccgac gcggtcaagg agcacttcgg caaggtcaac cagatctaca acaacgccgg catcgccttc accggcgacg tcgaggtcag ccagttcaag gacatcgaac gggtgatgga cgtcgacttc tggggcgtgg tcaacggcac aaaggcgttc ctgccgcacc tgatcgcctc cggcgacgga cacgtcgtca acgtctccag cgtgttcggc ctgttctcgg tgccgggcca ggcggcctac aactcggcca agttcgccgt gcgcggcttc accgaggcgc tgcgccagga gatggccgcg gccggtcacc cggtggcggt gacgaccgtg caccccggcg gcatcaagac cgcgatcgcc cgcaacgcca ccgcggccga gggcctggac caggccgagc tggccaagct gttcgacaag cggctggcca agaccacgcc gcagcgcgcc gcccagatca tcctggacgc ggtgcgcaag aagaaggcgc gggtgctggt cggctcggac gccaaggcgc tcgacatcct ggtgcggctg accggttcgg gctaccagcg gctgttcggg ccggtcatga gccggctgct gcccaacaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa