MypaA.01097.d

Putative uncharacterized protein

CENTER ID: MypaA.01097.d
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01097.d.A1.GE29540 Full length( MypaA.01097.d ) 1 283
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01097.d.A1.PS00737 Full length( MypaA.01097.d ) 1 283

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.2694
RefSeq: NP_961472.1
UniProt: Q73WX2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQGFAGKVAV VTGAGSGIGQ ALAVELGRAG AKLAISDVDT AGLAQTAEQL AAIGAPVKAD RLDVTEREAF LAYADAVNEH YGRVNQIYNN AGITFIGSIE DSRFKDIERV VDVDFWGVVN GTKAFLPHLI ASGDGHVINI SSALGLFSAP GQAAYVSAKF AVRGFTEALH QEMLRAGHPV RVTTVHPGGI KTAFARNATG VEGLDHAELA SLFEEQQAKT TPQRAAQLIL DGVRRNKARV LVGPDVKAMD LLVRAAGPNY ERLLAGPVMG RVKEFVTRLL PKR
NT Sequence
ATGCAAGGGT TCGCCGGCAA GGTCGCCGTG GTCACCGGCG CCGGATCGGG CATCGGGCAG GCGCTGGCGG TGGAGCTGGG CCGGGCCGGG GCCAAGCTGG CGATCAGCGA CGTGGACACC GCGGGGCTGG CGCAGACGGC GGAGCAGCTT GCCGCGATCG GCGCGCCGGT CAAGGCGGAT CGGCTCGACG TGACCGAACG CGAGGCGTTC CTGGCCTACG CCGACGCGGT CAACGAGCAT TACGGCCGGG TCAACCAGAT CTACAACAAC GCCGGCATCA CGTTCATCGG CTCCATCGAG GACAGCCGGT TCAAGGACAT CGAGCGGGTG GTCGACGTCG ACTTCTGGGG CGTGGTCAAC GGCACCAAGG CGTTCCTGCC CCACCTGATC GCCTCCGGCG ACGGACACGT GATCAACATC TCCAGCGCGC TCGGCCTGTT CTCGGCGCCC GGGCAGGCGG CCTACGTGTC GGCCAAGTTC GCCGTCCGCG GCTTCACCGA GGCGCTGCAT CAAGAGATGC TGCGCGCCGG TCACCCGGTC CGGGTCACCA CGGTGCACCC CGGCGGCATC AAAACCGCGT TCGCCCGCAA CGCCACCGGT GTCGAGGGCC TCGACCACGC CGAGCTGGCC AGCCTGTTCG AGGAACAGCA GGCCAAGACG ACGCCGCAGC GCGCCGCCCA GCTCATCCTG GACGGGGTGC GCCGCAACAA GGCCCGGGTG CTGGTCGGAC CCGACGTCAA GGCCATGGAC CTGCTGGTGC GGGCGGCCGG CCCCAACTAC GAGCGGCTGC TGGCCGGTCC GGTGATGGGC CGGGTCAAGG AGTTCGTGAC CCGGTTGCTG CCCAAGCGT
Details for MypaA.01097.d.A1.GE29540
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 32kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 112
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQGFAGKVA VVTGAGSGIG QALAVELGRA GAKLAISDVD TAGLAQTAEQ LAAIGAPVKA DRLDVTEREA FLAYADAVNE HYGRVNQIYN NAGITFIGSI EDSRFKDIER VVDVDFWGVV NGTKAFLPHL IASGDGHVIN ISSALGLFSA PGQAAYVSAK FAVRGFTEAL HQEMLRAGHP VRVTTVHPGG IKTAFARNAT GVEGLDHAEL ASLFEEQQAK TTPQRAAQLI LDGVRRNKAR VLVGPDVKAM DLLVRAAGPN YERLLAGPVM GRVKXFVTXV AAXALIXSTN XXLQPSFSXX XLLTKPEXKL XXLLXXXXXX X
Validated NT Sequence
attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgcaa gggttcgccg gcaaggtcgc cgtggtcacc ggcgccggat cgggcatcgg gcaggcgctg gcggtggagc tgggccgggc cggggccaag ctggcgatca gcgacgtgga caccgcgggg ctggcgcaga cggcggagca gcttgccgcg atcggcgcgc cggtcaaggc ggatcggctc gacgtgaccg aacgcgaggc gttcctggcc tacgccgacg cggtcaacga gcattacggc cgggtcaacc agatctacaa caacgccggc atcacgttca tcggctccat cgaggacagc cggttcaagg acatcgagcg ggtggtcgac gtcgacttct ggggcgtggt caacggcacc aaggcgttcc tgccccacct gatcgcctcc ggcgacggac acgtgatcaa catctccagc gcgctcggcc tgttctcggc gcccgggcag gcggcctacg tgtcggccaa gttcgccgtc cgcggcttca ccgaggcgct gcatcaagag atgctgcgcg ccggtcaccc ggtccgggtc accacggtgc accccggcgg catcaaaacc gcgttcgccc gcaacgccac cggtgtcgag ggcctcgacc acgccgagct ggccagcctg ttcgaggaac agcaggccaa gacgacgccg cagcgcgccg cccagctcat cctggacggg gtgcgccgca acaaggcccg ggtgctggtc ggacccgacg tcaaggccat ggacctgctg gtgcgggcgg ccggccccaa ctacgagcgg ctgctggccg gtccggtgat gggccgggtc aagganttcg tgacccnggt tgctgcccna gcgttaataa ncagcacgaa cnannttctg cagccnagct tctcnangan ncnnctgcta acaaagcccg aaangaagct gantnggctg ctgcncnnnn nnnnnnnnnn nnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQGFAGKVA VVTGAGSGIG QALAVELGRA GAKLAISDVD TAGLAQTAEQ LAAIGAPVKA DRLDVTEREA FLAYADAVNE HYGRVNQIYN NAGITFIGSI EDSRFKDIER VVDVDFWGVV NGTKAFLPHL IASGDGHVIN ISSALGLFSA PGQAAYVSAK FAVRGFTEAL HQEMLRAGHP VRVTTVHPGG IKTAFARNAT GVEGLDHAEL ASLFEEQQAK TTPQRAAQLI LDGVRRNKAR VLVGPDVKAM DLLVRAAGPN YERLLAGPVM GRVKEFVTRL LPKR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaagggttc gccggcaagg tcgccgtggt caccggcgcc ggatcgggca tcgggcaggc gctggcggtg gagctgggcc gggccggggc caagctggcg atcagcgacg tggacaccgc ggggctggcg cagacggcgg agcagcttgc cgcgatcggc gcgccggtca aggcggatcg gctcgacgtg accgaacgcg aggcgttcct ggcctacgcc gacgcggtca acgagcatta cggccgggtc aaccagatct acaacaacgc cggcatcacg ttcatcggct ccatcgagga cagccggttc aaggacatcg agcgggtggt cgacgtcgac ttctggggcg tggtcaacgg caccaaggcg ttcctgcccc acctgatcgc ctccggcgac ggacacgtga tcaacatctc cagcgcgctc ggcctgttct cggcgcccgg gcaggcggcc tacgtgtcgg ccaagttcgc cgtccgcggc ttcaccgagg cgctgcatca agagatgctg cgcgccggtc acccggtccg ggtcaccacg gtgcaccccg gcggcatcaa aaccgcgttc gcccgcaacg ccaccggtgt cgagggcctc gaccacgccg agctggccag cctgttcgag gaacagcagg ccaagacgac gccgcagcgc gccgcccagc tcatcctgga cggggtgcgc cgcaacaagg cccgggtgct ggtcggaccc gacgtcaagg ccatggacct gctggtgcgg gcggccggcc ccaactacga gcggctgctg gccggtccgg tgatgggccg ggtcaaggag ttcgtgaccc ggttgctgcc caagcgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MypaA.01097.d.A1.PS00737
PURIFICATION DATe: 8/26/2010
CONCENTRATION: 19.22mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 112
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQGFAGKVA VVTGAGSGIG QALAVELGRA GAKLAISDVD TAGLAQTAEQ LAAIGAPVKA DRLDVTEREA FLAYADAVNE HYGRVNQIYN NAGITFIGSI EDSRFKDIER VVDVDFWGVV NGTKAFLPHL IASGDGHVIN ISSALGLFSA PGQAAYVSAK FAVRGFTEAL HQEMLRAGHP VRVTTVHPGG IKTAFARNAT GVEGLDHAEL ASLFEEQQAK TTPQRAAQLI LDGVRRNKAR VLVGPDVKAM DLLVRAAGPN YERLLAGPVM GRVKXFVTXV AAXALIXSTN XXLQPSFSXX XLLTKPEXKL XXLLXXXXXX X
Validated NT Sequence
attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgcaa gggttcgccg gcaaggtcgc cgtggtcacc ggcgccggat cgggcatcgg gcaggcgctg gcggtggagc tgggccgggc cggggccaag ctggcgatca gcgacgtgga caccgcgggg ctggcgcaga cggcggagca gcttgccgcg atcggcgcgc cggtcaaggc ggatcggctc gacgtgaccg aacgcgaggc gttcctggcc tacgccgacg cggtcaacga gcattacggc cgggtcaacc agatctacaa caacgccggc atcacgttca tcggctccat cgaggacagc cggttcaagg acatcgagcg ggtggtcgac gtcgacttct ggggcgtggt caacggcacc aaggcgttcc tgccccacct gatcgcctcc ggcgacggac acgtgatcaa catctccagc gcgctcggcc tgttctcggc gcccgggcag gcggcctacg tgtcggccaa gttcgccgtc cgcggcttca ccgaggcgct gcatcaagag atgctgcgcg ccggtcaccc ggtccgggtc accacggtgc accccggcgg catcaaaacc gcgttcgccc gcaacgccac cggtgtcgag ggcctcgacc acgccgagct ggccagcctg ttcgaggaac agcaggccaa gacgacgccg cagcgcgccg cccagctcat cctggacggg gtgcgccgca acaaggcccg ggtgctggtc ggacccgacg tcaaggccat ggacctgctg gtgcgggcgg ccggccccaa ctacgagcgg ctgctggccg gtccggtgat gggccgggtc aagganttcg tgacccnggt tgctgcccna gcgttaataa ncagcacgaa cnannttctg cagccnagct tctcnangan ncnnctgcta acaaagcccg aaangaagct gantnggctg ctgcncnnnn nnnnnnnnnn nnnnna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQGFAGKVA VVTGAGSGIG QALAVELGRA GAKLAISDVD TAGLAQTAEQ LAAIGAPVKA DRLDVTEREA FLAYADAVNE HYGRVNQIYN NAGITFIGSI EDSRFKDIER VVDVDFWGVV NGTKAFLPHL IASGDGHVIN ISSALGLFSA PGQAAYVSAK FAVRGFTEAL HQEMLRAGHP VRVTTVHPGG IKTAFARNAT GVEGLDHAEL ASLFEEQQAK TTPQRAAQLI LDGVRRNKAR VLVGPDVKAM DLLVRAAGPN YERLLAGPVM GRVKEFVTRL LPKR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAAGGGTTC GCCGGCAAGG TCGCCGTGGT CACCGGCGCC GGATCGGGCA TCGGGCAGGC GCTGGCGGTG GAGCTGGGCC GGGCCGGGGC CAAGCTGGCG ATCAGCGACG TGGACACCGC GGGGCTGGCG CAGACGGCGG AGCAGCTTGC CGCGATCGGC GCGCCGGTCA AGGCGGATCG GCTCGACGTG ACCGAACGCG AGGCGTTCCT GGCCTACGCC GACGCGGTCA ACGAGCATTA CGGCCGGGTC AACCAGATCT ACAACAACGC CGGCATCACG TTCATCGGCT CCATCGAGGA CAGCCGGTTC AAGGACATCG AGCGGGTGGT CGACGTCGAC TTCTGGGGCG TGGTCAACGG CACCAAGGCG TTCCTGCCCC ACCTGATCGC CTCCGGCGAC GGACACGTGA TCAACATCTC CAGCGCGCTC GGCCTGTTCT CGGCGCCCGG GCAGGCGGCC TACGTGTCGG CCAAGTTCGC CGTCCGCGGC TTCACCGAGG CGCTGCATCA AGAGATGCTG CGCGCCGGTC ACCCGGTCCG GGTCACCACG GTGCACCCCG GCGGCATCAA AACCGCGTTC GCCCGCAACG CCACCGGTGT CGAGGGCCTC GACCACGCCG AGCTGGCCAG CCTGTTCGAG GAACAGCAGG CCAAGACGAC GCCGCAGCGC GCCGCCCAGC TCATCCTGGA CGGGGTGCGC CGCAACAAGG CCCGGGTGCT GGTCGGACCC GACGTCAAGG CCATGGACCT GCTGGTGCGG GCGGCCGGCC CCAACTACGA GCGGCTGCTG GCCGGTCCGG TGATGGGCCG GGTCAAGGAG TTCGTGACCC GGTTGCTGCC CAAGCGTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA