SSGCID: Target Page

MypaA.01007.a

Putative uncharacterized protein

CENTER ID:	MypaA.01007.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01007.a.A1.GE29529	Full length( MypaA.01007.a )		1	198

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.3289
RefSeq:	NP_962038.1
UniProt:	Q73VB0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRGWTPTTEG NWRIVAIKIL ALVGSLRSAS INRQIAELAS AVAGEDVVVT VFEGLGELPF YNEEIDDAMT SDAPPLAPVA ALRAAAADAD AALVVTPEYN GSIPAVIKNA IDWLSRPFGD GALKGKPLAV IGGSFGQYGG VWAHDETRKS FGIAGARVVE SIKLSVPFKT LAGKAPAEHA ELSANVRDVV GKLAAEVG

NT Sequence

GTGCGAGGAT GGACACCAAC AACCGAAGGG AACTGGCGAA TCGTGGCAAT CAAAATCTTG GCATTAGTGG GAAGCCTGCG CTCGGCGTCG ATCAACCGCC AGATCGCGGA GCTGGCGAGC GCTGTCGCCG GCGAGGACGT CGTCGTCACC GTCTTCGAGG GGCTCGGCGA GCTGCCGTTC TACAACGAGG AGATCGACGA CGCGATGACC AGCGACGCGC CGCCGCTGGC CCCGGTGGCC GCGCTGCGCG CCGCGGCGGC CGACGCCGAC GCCGCGCTGG TGGTCACCCC CGAGTACAAC GGCAGCATCC CGGCGGTCAT CAAGAACGCG ATCGACTGGT TGTCCCGCCC GTTCGGCGAC GGCGCGCTGA AGGGCAAGCC GCTGGCCGTC ATCGGGGGGT CTTTCGGCCA GTACGGGGGA GTGTGGGCGC ACGACGAGAC CCGCAAGTCC TTCGGGATCG CCGGGGCGCG GGTGGTCGAG TCGATCAAGC TGTCCGTGCC GTTCAAGACG CTGGCCGGCA AGGCGCCCGC CGAGCATGCC GAACTGTCGG CGAACGTGCG CGACGTGGTC GGCAAGCTGG CCGCCGAGGT CGGC

Details for MypaA.01007.a.A1.GE29529

HARVESTED ON:	6/15/2010
SEQUENCED ON:	6/27/2010
EXPECTED MW:	22kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVRGWTPTT EGNWRIVAIK ILALVGSLRS ASINRQIAEL ASAVAGEDVV VTVFEGLGEL PFYNEEIDDA MTSDAPPLAP VAALRAAAAD ADAALVVTPE YNGSIPAVIK NAIDWLSRPF GDGALKGKPL AVIGGSFGQY GGVWAHDETR KSFGIAGARV VESIKLSVPF KTLAGKAPAE HAELSANVRD VVGKLAAEVG

Validated NT Sequence

attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc gaggatggac accaacaacc gaagggaact ggcgaatcgt ggcaatcaaa atcttggcat tagtgggaag cctgcgctcg gcgtcgatca accgccagat cgcggagctg gcgagcgctg tcgccggcga ggacgtcgtc gtcaccgtct tcgaggggct cggcgagctg ccgttctaca acgaggagat cgacgacgcg atgaccagcg acgcgccgcc gctggccccg gtggccgcgc tgcgcgccgc ggcggccgac gccgacgccg cgctggtggt cacccccgag tacaacggca gcatcccggc ggtcatcaag aacgcgatcg actggttgtc ccgcccgttc ggcgacggcg cgctgaaggg caagccgctg gccgtcatcg gggggtcttt cggccagtac gggggagtgt gggcgcacga cgagacccgc aagtccttcg ggatcgccgg ggcgcgggtg gtcgagtcga tcaagctgtc cgtgccgttc aagacgctgg ccggcaaggc gcccgccgag catgccgaac tgtcggcgaa cgtgcgcgac gtggtcggca agctggccgc cgaggtcggc taataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc acc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcgagga tggacaccaa caaccgaagg gaactggcga atcgtggcaa tcaaaatctt ggcattagtg ggaagcctgc gctcggcgtc gatcaaccgc cagatcgcgg agctggcgag cgctgtcgcc ggcgaggacg tcgtcgtcac cgtcttcgag gggctcggcg agctgccgtt ctacaacgag gagatcgacg acgcgatgac cagcgacgcg ccgccgctgg ccccggtggc cgcgctgcgc gccgcggcgg ccgacgccga cgccgcgctg gtggtcaccc ccgagtacaa cggcagcatc ccggcggtca tcaagaacgc gatcgactgg ttgtcccgcc cgttcggcga cggcgcgctg aagggcaagc cgctggccgt catcgggggg tctttcggcc agtacggggg agtgtgggcg cacgacgaga cccgcaagtc cttcgggatc gccggggcgc gggtggtcga gtcgatcaag ctgtccgtgc cgttcaagac gctggccggc aaggcgcccg ccgagcatgc cgaactgtcg gcgaacgtgc gcgacgtggt cggcaagctg gccgccgagg tcggcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa