MypaA.00951.d

Putative uncharacterized protein

CENTER ID: MypaA.00951.d
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00951.d.A1.GE29528 Full length( MypaA.00951.d ) 1 498
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.616
RefSeq: NP_959515.1
UniProt: Q743K4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLDEYMALD ATALAGLVAD KQVTPAELLA LARQRADAVN PRLNAIVCRL DDVADRQAAD PNLSGPFAGV PFLLKDLGQE YRGFPTSCGS RSLANDVADR HALVTQRFLD AGLVIFGKTN TPEFGAKGAT EPDYWGPARN PWDTERTPGG SSGGSGAAVA AGIVPAAGAN DGGGSIRIPA ACNGLVGLKP SRGLAPYGPQ TGELMLGMAT QGVVTRTVRD SAALLDAIIG HHPDASYRVA LPDKPFAEQI TRPPGPLTIG YSARSALNPR PDREAVVAVE NSAALLSELG HRVREVAPPY DDAALGRDFL TIWFAQLHQQ VADIRRRTGA RDNDFEADTL AISELGRSGG VLPVMSALEN RNTYIQSLAA FHEGYDFFLT PTLATPPLVV GATSTSAALQ RAARLVSRVR GGKLMRLSGI LDDLIRESLG WVPYTQLANL TGRPAISVPL HWTDDGLPLG VQFVGKLGAD GDLLQLAAQL EQARPWAQRH PAPVTPPN
NT Sequence
ATGCGTCTGG ACGAGTACAT GGCGTTGGAT GCGACCGCCC TGGCCGGGCT GGTGGCGGAC AAGCAGGTCA CGCCGGCCGA ACTGCTCGCG CTGGCACGGC AGCGCGCGGA CGCGGTGAAT CCGCGCCTGA ACGCCATCGT GTGCCGACTC GACGACGTCG CCGACCGGCA AGCCGCCGAC CCGAACCTGA GCGGGCCGTT CGCCGGGGTG CCGTTCCTGC TCAAGGACCT CGGCCAGGAG TATCGCGGCT TTCCCACCTC GTGCGGATCA CGGTCGCTGG CCAACGACGT CGCCGACCGG CACGCGCTGG TCACCCAGCG GTTCCTGGAC GCGGGGCTGG TGATCTTCGG CAAGACCAAT ACGCCCGAGT TCGGGGCCAA GGGGGCGACC GAGCCCGATT ACTGGGGACC CGCCCGCAAT CCGTGGGACA CCGAACGCAC CCCCGGCGGC TCGTCGGGCG GATCCGGCGC GGCGGTGGCG GCCGGCATTG TCCCGGCCGC GGGCGCCAAC GACGGTGGCG GGTCGATCCG CATCCCGGCC GCCTGCAACG GCCTCGTCGG CCTCAAGCCC AGCCGGGGGT TGGCGCCCTA CGGCCCGCAG ACCGGCGAGC TGATGCTGGG CATGGCCACC CAGGGTGTGG TCACCCGCAC GGTGCGCGAC AGCGCCGCGC TGCTGGACGC GATCATCGGG CACCACCCCG ACGCGTCCTA CCGAGTTGCG CTGCCGGACA AGCCCTTTGC CGAACAGATC ACGCGCCCTC CGGGACCTCT GACAATCGGG TACTCGGCCC GTTCGGCGCT CAACCCGCGC CCGGACCGCG AGGCCGTCGT CGCCGTCGAG AACAGTGCGG CCCTGCTGAG CGAGTTAGGC CACCGGGTGC GCGAGGTTGC CCCGCCTTAC GACGACGCCG CGCTGGGGCG TGACTTCCTG ACCATCTGGT TCGCCCAGCT GCACCAGCAG GTCGCCGACA TCAGGAGGCG AACCGGGGCC CGCGACAACG ACTTCGAGGC CGACACCTTG GCAATCAGTG AGCTCGGCCG GTCCGGCGGG GTGCTGCCGG TGATGTCCGC CCTGGAGAAC AGGAACACCT ACATCCAGTC GCTGGCGGCG TTCCACGAGG GCTACGACTT CTTCCTCACG CCCACCCTGG CCACCCCTCC CCTGGTGGTC GGTGCCACCT CGACGTCGGC GGCGCTGCAG CGCGCGGCCC GCCTGGTCAG CAGGGTGCGC GGCGGTAAAC TGATGCGGCT CAGCGGGATT CTCGATGACC TCATCCGGGA GAGCCTGGGC TGGGTGCCCT ACACGCAGCT GGCCAACCTG ACCGGGCGGC CCGCGATCAG CGTGCCGCTG CACTGGACCG ACGACGGCCT TCCGCTCGGT GTGCAGTTCG TCGGGAAGCT CGGCGCCGAC GGGGATCTGC TGCAACTGGC CGCGCAACTC GAGCAGGCCC GGCCGTGGGC GCAGCGCCAC CCGGCGCCGG TCACCCCACC GAAT
Details for MypaA.00951.d.A1.GE29528
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 55kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 77
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLDEYMAL DATALAGLVA DKQVTPAELL ALARQRADAV NPRLNAIVCR LDDVADRQAA DPNLSGPFAG VPFLLKDLGQ EYRGFPTSCG SRSLANDVAD RHALVTQRFL DAGLVIFGKT NTPEFGAKGA TEPDYWGPAR NPWDTERTPG GSSGGSGAAV AAGIVPAAGA NDGGGSIRIP AACNGLVGLK PSRGLAPYGP QTGELMLGMA TQGVVTRTVR DSAALLDAII GHHPDASYRV ALPXKPFAEQ ITRPXGPLTI GYSARSALNP RPXREXVVXV XXXCGPAXXV RPPGXXXXPX LTTTPXLGXD FLTSGXPSCX XRSXXSXXXX XXXXXXXXXX XXXXXXXXXV XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
ttttgtttac tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgcgtct ggacgagtac atggcgttgg atgcgaccgc cctggccggg ctggtggcgg acaagcaggt cacgccggcc gaactgctcg cgctggcacg gcagcgcgcg gacgcggtga atccgcgcct gaacgccatc gtgtgccgac tcgacgacgt cgccgaccgg caagccgccg acccgaacct gagcgggccg ttcgccgggg tgccgttcct gctcaaggac ctcggccagg agtatcgcgg ctttcccacc tcgtgcggat cacggtcgct ggccaacgac gtcgccgacc ggcacgcgct ggtcacccag cggttcctgg acgcggggct ggtgatcttc ggcaagacca atacgcccga gttcggggcc aagggggcga ccgagcccga ttactgggga cccgcccgca atccgtggga caccgaacgc acccccggcg gctcgtcggg cggatccggc gcggcggtgg cggccggcat tgtcccggcc gcgggcgcca acgacggtgg cgggtcgatc cgcatcccgg ccgcctgcaa cggcctcgtc ggcctcaagc ccagccgggg gttggcgccc tacggcccgc agaccggcga gctgatgctg ggcatggcca cccagggtgt ggtcacccgc acggtgcgcg acagcgccgc gctgctggac gcgatcatcg ggcaccaccc cgacgcgtcc taccgagttg cgctgccgga naagcccttt gccgaacaga tcacgcgccc tncgggacct ctgacaatcg ggtactcggc ccgttcggcg ctcaacccgc gcccggancg ngaggncgtc gtcnccgtcn annannagtg cggncctgct ganngagtta ggccnccggg ngnncnangn ngccccnncc ttacgacgac gccncnnctg gggnnngact tcctgacntc tggnncgccc agctgcnncn nnaggtcgnc nanntcnnna nggnnccnnn acnacnnnnn nanncnannn nnnngnnanc annnagnnnn ngnngnnnnn nnnnnngtcn nccnnnnnaa nnnnnnnncc nnnnnnngnn nnnnnnnnnn ngnnnnnant nnnnnnnnnn nnnnncnnnn nnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLDEYMAL DATALAGLVA DKQVTPAELL ALARQRADAV NPRLNAIVCR LDDVADRQAA DPNLSGPFAG VPFLLKDLGQ EYRGFPTSCG SRSLANDVAD RHALVTQRFL DAGLVIFGKT NTPEFGAKGA TEPDYWGPAR NPWDTERTPG GSSGGSGAAV AAGIVPAAGA NDGGGSIRIP AACNGLVGLK PSRGLAPYGP QTGELMLGMA TQGVVTRTVR DSAALLDAII GHHPDASYRV ALPDKPFAEQ ITRPPGPLTI GYSARSALNP RPDREAVVAV ENSAALLSEL GHRVREVAPP YDDAALGRDF LTIWFAQLHQ QVADIRRRTG ARDNDFEADT LAISELGRSG GVLPVMSALE NRNTYIQSLA AFHEGYDFFL TPTLATPPLV VGATSTSAAL QRAARLVSRV RGGKLMRLSG ILDDLIRESL GWVPYTQLAN LTGRPAISVP LHWTDDGLPL GVQFVGKLGA DGDLLQLAAQ LEQARPWAQR HPAPVTPPN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtctggac gagtacatgg cgttggatgc gaccgccctg gccgggctgg tggcggacaa gcaggtcacg ccggccgaac tgctcgcgct ggcacggcag cgcgcggacg cggtgaatcc gcgcctgaac gccatcgtgt gccgactcga cgacgtcgcc gaccggcaag ccgccgaccc gaacctgagc gggccgttcg ccggggtgcc gttcctgctc aaggacctcg gccaggagta tcgcggcttt cccacctcgt gcggatcacg gtcgctggcc aacgacgtcg ccgaccggca cgcgctggtc acccagcggt tcctggacgc ggggctggtg atcttcggca agaccaatac gcccgagttc ggggccaagg gggcgaccga gcccgattac tggggacccg cccgcaatcc gtgggacacc gaacgcaccc ccggcggctc gtcgggcgga tccggcgcgg cggtggcggc cggcattgtc ccggccgcgg gcgccaacga cggtggcggg tcgatccgca tcccggccgc ctgcaacggc ctcgtcggcc tcaagcccag ccgggggttg gcgccctacg gcccgcagac cggcgagctg atgctgggca tggccaccca gggtgtggtc acccgcacgg tgcgcgacag cgccgcgctg ctggacgcga tcatcgggca ccaccccgac gcgtcctacc gagttgcgct gccggacaag ccctttgccg aacagatcac gcgccctccg ggacctctga caatcgggta ctcggcccgt tcggcgctca acccgcgccc ggaccgcgag gccgtcgtcg ccgtcgagaa cagtgcggcc ctgctgagcg agttaggcca ccgggtgcgc gaggttgccc cgccttacga cgacgccgcg ctggggcgtg acttcctgac catctggttc gcccagctgc accagcaggt cgccgacatc aggaggcgaa ccggggcccg cgacaacgac ttcgaggccg acaccttggc aatcagtgag ctcggccggt ccggcggggt gctgccggtg atgtccgccc tggagaacag gaacacctac atccagtcgc tggcggcgtt ccacgagggc tacgacttct tcctcacgcc caccctggcc acccctcccc tggtggtcgg tgccacctcg acgtcggcgg cgctgcagcg cgcggcccgc ctggtcagca gggtgcgcgg cggtaaactg atgcggctca gcgggattct cgatgacctc atccgggaga gcctgggctg ggtgccctac acgcagctgg ccaacctgac cgggcggccc gcgatcagcg tgccgctgca ctggaccgac gacggccttc cgctcggtgt gcagttcgtc gggaagctcg gcgccgacgg ggatctgctg caactggccg cgcaactcga gcaggcccgg ccgtgggcgc agcgccaccc ggcgccggtc accccaccga ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa