MypaA.00951.c

AmiA2

CENTER ID: MypaA.00951.c
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00951.c.A1.GE29527 Full length( MypaA.00951.c ) 1 503
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.2278
RefSeq: NP_961077.1
UniProt: Q73Y15

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIGASESGPG ALSGSGSGSG NQRLPTLTDL LYQLASRSVS STTLVGRSLH AIHASQPTLN AFRVVLTESA LADAAEADRR RAAGDTAPLL GIPIAVKDDV DIAGVPTAFG TEGSVPPATH DAEVVRRLKA AGAVIVGKTN TCELGQWPFT SGPGFGHTRN PWSRRHTPGG SSGGSAAAVA AGLVTAAIGS DGAGSIRIPA AWTHLVGIKP QRGRISTWPL PEAFNGITVN GVLARTVADA ALVLDAASGN VDGDRHKPPP ITASDYVGKA PGPLNIALST RFPYTGFRPK LHPEILAATR AVGKQLELLG HTVVPGNPDY GMRLSWDFLA RSTAGLRDWE ERLGDGVVLD PRTVANLRLG HLLGQAILRS ARRHEAADQR RVGSIFDIVD VVLAPTTAQP PPLARAFDRL SGFGTDRAMI AACPLTFPWN VLGWPSINVP AGFTSDGLPI GVQLMGPANS EGMLISLAAE LEAVSGWASK QPQPWWDQSA DTPPAPGQPA PTG
NT Sequence
GTGATTGGCG CTTCTGAGTC GGGTCCCGGG GCGCTTTCCG GCTCGGGTTC CGGATCCGGC AACCAGCGCC TGCCCACCCT GACTGACCTG CTGTATCAGC TGGCCAGCCG GTCGGTGAGT TCCACCACAC TGGTGGGTCG CTCGCTGCAC GCTATCCATG CCAGCCAGCC CACGCTGAAC GCCTTCCGGG TGGTGCTCAC CGAGTCGGCG CTGGCCGACG CCGCCGAGGC CGATCGGCGC CGCGCGGCCG GGGACACCGC CCCGCTGCTG GGCATTCCGA TCGCCGTCAA GGACGACGTC GACATTGCCG GCGTGCCCAC CGCCTTCGGC ACCGAGGGCT CGGTGCCGCC CGCCACGCAC GATGCCGAGG TGGTTCGCCG CCTCAAGGCC GCCGGCGCGG TGATCGTCGG CAAAACCAAC ACCTGTGAAC TCGGGCAATG GCCGTTCACC AGCGGACCGG GCTTCGGGCA CACCCGCAAT CCCTGGTCGC GCCGGCACAC CCCGGGCGGA TCGTCGGGCG GCAGCGCCGC GGCGGTGGCC GCGGGCCTGG TGACCGCCGC CATCGGGTCC GACGGCGCCG GCAGCATCCG CATCCCGGCG GCGTGGACGC ACCTGGTGGG CATCAAGCCG CAGCGCGGAC GCATCTCGAC CTGGCCGCTG CCGGAGGCGT TCAACGGGAT CACGGTCAAC GGCGTGCTGG CCCGCACGGT GGCCGACGCG GCGTTGGTGC TCGACGCGGC GTCCGGCAAC GTCGACGGCG ACCGGCACAA ACCGCCCCCG ATCACCGCCT CCGACTATGT GGGCAAGGCG CCCGGTCCGT TGAACATCGC GCTGTCGACC CGGTTCCCCT ACACGGGGTT CCGGCCGAAG CTGCATCCGG AGATCCTGGC CGCGACCCGT GCGGTGGGCA AGCAACTCGA GCTGCTGGGC CACACCGTGG TGCCGGGCAA CCCGGACTAC GGCATGCGGC TGTCGTGGGA TTTTCTCGCC CGCTCCACCG CCGGCCTGCG GGACTGGGAG GAGCGGTTGG GCGACGGCGT GGTTCTGGAC CCCCGCACGG TGGCCAACTT GCGGCTGGGT CATCTGCTGG GACAGGCGAT CCTGCGCAGC GCCCGCCGCC ACGAGGCCGC CGACCAGCGC CGGGTGGGCT CGATCTTCGA CATCGTCGAC GTGGTGCTGG CGCCGACCAC CGCGCAACCC CCGCCGCTGG CCCGCGCCTT CGACCGGCTC AGCGGCTTCG GCACCGACCG CGCCATGATC GCGGCGTGCC CGCTGACCTT CCCGTGGAAC GTGCTGGGCT GGCCGTCGAT CAACGTTCCG GCCGGCTTCA CCTCCGACGG CCTGCCGATC GGCGTGCAGC TGATGGGCCC GGCCAACAGC GAGGGCATGC TGATCTCGCT GGCCGCCGAG TTGGAGGCGG TGTCCGGGTG GGCGAGCAAA CAGCCGCAGC CCTGGTGGGA CCAGAGCGCC GACACTCCCC CCGCCCCCGG CCAGCCCGCG CCCACCGGG
Details for MypaA.00951.c.A1.GE29527
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 24
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVIGASESG PGALSGSGSG SGNQRLPTLT DLLYQLASRS VSSTTLVGRS LHAIHASQPT LNAFRVVLTE SALADAAXXD RXRAAGXTAP LLGIPIAVXX XXXIAGV
Validated NT Sequence
gggcacgccg gcaatgtnga ngtnntcctn gacggcgatc ggaatgccca gcagcggggc ggtgtnnccg gccgcgcggc nccgatcgnc ntnggcggcg tcggccagcg ccgactcggt gagcaccacc cggaaggcgt tcagcgtggg ctggctggca tggatagcgt gcagcgagcg acccaccagt gtggtggaac tcaccgaccg gctggccagc tgatacagca ggtcagtcag ggtgggcagg cgctggttgc cggatccgga acccgagccg gaaagcgccc cgggacccga ctcagaagcg ccaatcacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVIGASESG PGALSGSGSG SGNQRLPTLT DLLYQLASRS VSSTTLVGRS LHAIHASQPT LNAFRVVLTE SALADAAEAD RRRAAGDTAP LLGIPIAVKD DVDIAGVPTA FGTEGSVPPA THDAEVVRRL KAAGAVIVGK TNTCELGQWP FTSGPGFGHT RNPWSRRHTP GGSSGGSAAA VAAGLVTAAI GSDGAGSIRI PAAWTHLVGI KPQRGRISTW PLPEAFNGIT VNGVLARTVA DAALVLDAAS GNVDGDRHKP PPITASDYVG KAPGPLNIAL STRFPYTGFR PKLHPEILAA TRAVGKQLEL LGHTVVPGNP DYGMRLSWDF LARSTAGLRD WEERLGDGVV LDPRTVANLR LGHLLGQAIL RSARRHEAAD QRRVGSIFDI VDVVLAPTTA QPPPLARAFD RLSGFGTDRA MIAACPLTFP WNVLGWPSIN VPAGFTSDGL PIGVQLMGPA NSEGMLISLA AELEAVSGWA SKQPQPWWDQ SADTPPAPGQ PAPTG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgattggc gcttctgagt cgggtcccgg ggcgctttcc ggctcgggtt ccggatccgg caaccagcgc ctgcccaccc tgactgacct gctgtatcag ctggccagcc ggtcggtgag ttccaccaca ctggtgggtc gctcgctgca cgctatccat gccagccagc ccacgctgaa cgccttccgg gtggtgctca ccgagtcggc gctggccgac gccgccgagg ccgatcggcg ccgcgcggcc ggggacaccg ccccgctgct gggcattccg atcgccgtca aggacgacgt cgacattgcc ggcgtgccca ccgccttcgg caccgagggc tcggtgccgc ccgccacgca cgatgccgag gtggttcgcc gcctcaaggc cgccggcgcg gtgatcgtcg gcaaaaccaa cacctgtgaa ctcgggcaat ggccgttcac cagcggaccg ggcttcgggc acacccgcaa tccctggtcg cgccggcaca ccccgggcgg atcgtcgggc ggcagcgccg cggcggtggc cgcgggcctg gtgaccgccg ccatcgggtc cgacggcgcc ggcagcatcc gcatcccggc ggcgtggacg cacctggtgg gcatcaagcc gcagcgcgga cgcatctcga cctggccgct gccggaggcg ttcaacggga tcacggtcaa cggcgtgctg gcccgcacgg tggccgacgc ggcgttggtg ctcgacgcgg cgtccggcaa cgtcgacggc gaccggcaca aaccgccccc gatcaccgcc tccgactatg tgggcaaggc gcccggtccg ttgaacatcg cgctgtcgac ccggttcccc tacacggggt tccggccgaa gctgcatccg gagatcctgg ccgcgacccg tgcggtgggc aagcaactcg agctgctggg ccacaccgtg gtgccgggca acccggacta cggcatgcgg ctgtcgtggg attttctcgc ccgctccacc gccggcctgc gggactggga ggagcggttg ggcgacggcg tggttctgga cccccgcacg gtggccaact tgcggctggg tcatctgctg ggacaggcga tcctgcgcag cgcccgccgc cacgaggccg ccgaccagcg ccgggtgggc tcgatcttcg acatcgtcga cgtggtgctg gcgccgacca ccgcgcaacc cccgccgctg gcccgcgcct tcgaccggct cagcggcttc ggcaccgacc gcgccatgat cgcggcgtgc ccgctgacct tcccgtggaa cgtgctgggc tggccgtcga tcaacgttcc ggccggcttc acctccgacg gcctgccgat cggcgtgcag ctgatgggcc cggccaacag cgagggcatg ctgatctcgc tggccgccga gttggaggcg gtgtccgggt gggcgagcaa acagccgcag ccctggtggg accagagcgc cgacactccc cccgcccccg gccagcccgc gcccaccggg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa