MypaA.00950.a

LpqL_2

CENTER ID: MypaA.00950.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00950.a.A1.GE29523 Full length( MypaA.00950.a ) 1 477
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.4158
RefSeq: NP_962841.1
UniProt: Q73T13

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLAGCSSAG PGLPTAAPDL GHGLAKKVTA DAMMAHLRAL QNIANTNGGN RADGTPGFQA SVDYVAKALR DKGFDVQTPQ FDRLYPVSPG KPTLTVAGRG YPVDQASMLV QTPPGGLTGP AVRPAQPSGC AASDYPGALP KGAIAVVDDT RCSVVDKQNS AVARGASALI VVSAPDGHGA PPTLFTPGYY KQLSVPVAVV NPSGAAALAG AGGPVRLILD AVNVKVTSRN VVAQTKTGSA HEVVMVGAHL DGSRAGPGIN DNGSGVAAVL ETALQLGPLA PVSNAVRFVF WGAGEDGLGG VMDYLFGLDR DQLNDIALYL NFTMLGSPNA GFFTDDGDQS GPPAPGISAA DVPEGSAGIE RTLAGYLNLA GKRAADMPLN TRADYHPFMV AGVPIGGMTT GASQPKTTVQ SRLWGGQAGV PFDPNFQSPR DTADNINRDA LAVMGSGVGF AVGSYAQSIG GVNGVPPHDK RHRTRVP
NT Sequence
GTGCTGCTGG CCGGTTGCTC GTCGGCGGGG CCCGGACTGC CGACCGCGGC ACCCGATTTG GGCCACGGCC TGGCCAAGAA GGTGACCGCC GACGCCATGA TGGCCCACCT GCGCGCCTTG CAGAACATCG CCAACACCAA CGGCGGCAAC CGCGCGGACG GCACCCCGGG CTTTCAGGCC AGCGTCGACT ACGTGGCTAA AGCGCTGCGC GACAAGGGTT TCGACGTACA GACGCCGCAG TTCGACCGGC TCTACCCGGT GTCCCCCGGC AAGCCGACGC TGACCGTCGC GGGCCGCGGC TACCCCGTCG ACCAGGCCTC GATGTTGGTC CAGACCCCGC CCGGCGGGCT GACCGGACCC GCCGTCCGCC CGGCCCAGCC GTCGGGTTGC GCGGCCAGCG ACTACCCCGG CGCCCTGCCC AAGGGTGCGA TCGCCGTCGT CGACGACACC CGCTGCTCGG TGGTCGACAA ACAGAACAGC GCGGTGGCCC GGGGCGCGAG CGCGCTGATC GTGGTGAGCG CACCCGACGG GCACGGCGCC CCGCCCACCT TGTTCACCCC CGGCTACTAC AAACAGCTGA GTGTGCCGGT GGCCGTCGTC AACCCGTCCG GCGCCGCCGC GCTGGCCGGT GCAGGCGGGC CGGTGCGGTT AATCCTGGAT GCAGTCAACG TCAAGGTCAC GTCGCGAAAC GTGGTGGCGC AGACCAAGAC CGGGTCAGCC CACGAGGTCG TCATGGTCGG TGCGCACCTG GACGGCTCGC GCGCCGGACC GGGCATCAAC GACAACGGCT CCGGCGTGGC CGCGGTGCTG GAAACCGCGC TGCAGCTGGG CCCGCTGGCG CCGGTAAGCA ACGCGGTGCG GTTCGTGTTC TGGGGCGCCG GGGAAGACGG ACTGGGCGGC GTGATGGATT ACCTGTTCGG CCTGGACCGC GACCAACTCA ACGACATCGC GCTGTATCTG AACTTCACCA TGCTCGGCTC GCCCAACGCC GGGTTCTTCA CCGACGACGG CGATCAGTCC GGGCCGCCGG CCCCCGGTAT TTCGGCCGCC GACGTTCCCG AAGGGTCGGC CGGCATCGAG CGCACCCTGG CGGGCTATCT GAACCTGGCC GGGAAGCGTG CGGCGGACAT GCCCCTGAAC ACCCGGGCCG ACTACCACCC CTTCATGGTG GCCGGTGTGC CGATCGGCGG CATGACCACC GGAGCCTCGC AACCGAAAAC CACTGTGCAG TCCCGGTTGT GGGGCGGCCA GGCCGGCGTG CCGTTCGATC CGAACTTCCA AAGCCCCCGC GACACCGCCG ACAACATCAA CCGGGACGCC CTGGCGGTGA TGGGTTCCGG CGTCGGGTTC GCGGTTGGCA GCTACGCCCA ATCGATCGGC GGGGTCAACG GGGTGCCGCC GCACGACAAG CGGCATCGCA CCCGAGTGCC C
Details for MypaA.00950.a.A1.GE29523
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 60
PERCENT COVERAGE: 23
Validated AA Sequence
MAHXHHHHMG TLEAXTQGPG SMVLLAGCSS AGXGLPTAXP DLXHGLAXXV TAXXMMAHLR ALXNIXNTXG GXRXXXXPXF XAXXXYVAXX LXXXXFXXXT XXXXXXXPXX XXXQX
Validated NT Sequence
anntttgttt nnttnnnaag ganatntacc atggctcatc ancatcacca tcatatgggt accctggaag ctcanaccca gggtcctggt tcgatggtgc tgctggccgg ttgctcgtcg gcggggnnng gactgccgac cgcgncnccc gatttgngcc acggnctggc cnanaangtg accgccnacg ncatgatggc ccacctgcgc gccttgcana acatcnncaa caccnacggc ggcnaccgcn nnnnnnncnc cccnancttt cnngccannn annantacgt ggctnnnnnn ctgnnnnnnn nngntttcna cgnncnnacn nnnnnnnnnn anngtntctn cccggngnnn nncnnnnngc aannctgacc gtcgcgggcc gcggntaccc cgncnaccan gcctcgatgt tggtccagac nnnnncnggn ggcctgaccg gacccgncnn cngcccggcc cagccgtcgg gnntgnncgg ccagnnnnnn nncnngngcc ctgcccaggn gcgatcgccn ncntcnacga caccngctgc tcngngnnnn annaanaana nngcgnngnn nnnnggcgcg agcncnntga tcnngntgan cnnncccacn ngnncngnnn nnnnncnnnn nngntccncc cccngctact acaaacngct ganngtgc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLLAGCSS AGPGLPTAAP DLGHGLAKKV TADAMMAHLR ALQNIANTNG GNRADGTPGF QASVDYVAKA LRDKGFDVQT PQFDRLYPVS PGKPTLTVAG RGYPVDQASM LVQTPPGGLT GPAVRPAQPS GCAASDYPGA LPKGAIAVVD DTRCSVVDKQ NSAVARGASA LIVVSAPDGH GAPPTLFTPG YYKQLSVPVA VVNPSGAAAL AGAGGPVRLI LDAVNVKVTS RNVVAQTKTG SAHEVVMVGA HLDGSRAGPG INDNGSGVAA VLETALQLGP LAPVSNAVRF VFWGAGEDGL GGVMDYLFGL DRDQLNDIAL YLNFTMLGSP NAGFFTDDGD QSGPPAPGIS AADVPEGSAG IERTLAGYLN LAGKRAADMP LNTRADYHPF MVAGVPIGGM TTGASQPKTT VQSRLWGGQA GVPFDPNFQS PRDTADNINR DALAVMGSGV GFAVGSYAQS IGGVNGVPPH DKRHRTRVP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgctgctg gccggttgct cgtcggcggg gcccggactg ccgaccgcgg cacccgattt gggccacggc ctggccaaga aggtgaccgc cgacgccatg atggcccacc tgcgcgcctt gcagaacatc gccaacacca acggcggcaa ccgcgcggac ggcaccccgg gctttcaggc cagcgtcgac tacgtggcta aagcgctgcg cgacaagggt ttcgacgtac agacgccgca gttcgaccgg ctctacccgg tgtcccccgg caagccgacg ctgaccgtcg cgggccgcgg ctaccccgtc gaccaggcct cgatgttggt ccagaccccg cccggcgggc tgaccggacc cgccgtccgc ccggcccagc cgtcgggttg cgcggccagc gactaccccg gcgccctgcc caagggtgcg atcgccgtcg tcgacgacac ccgctgctcg gtggtcgaca aacagaacag cgcggtggcc cggggcgcga gcgcgctgat cgtggtgagc gcacccgacg ggcacggcgc cccgcccacc ttgttcaccc ccggctacta caaacagctg agtgtgccgg tggccgtcgt caacccgtcc ggcgccgccg cgctggccgg tgcaggcggg ccggtgcggt taatcctgga tgcagtcaac gtcaaggtca cgtcgcgaaa cgtggtggcg cagaccaaga ccgggtcagc ccacgaggtc gtcatggtcg gtgcgcacct ggacggctcg cgcgccggac cgggcatcaa cgacaacggc tccggcgtgg ccgcggtgct ggaaaccgcg ctgcagctgg gcccgctggc gccggtaagc aacgcggtgc ggttcgtgtt ctggggcgcc ggggaagacg gactgggcgg cgtgatggat tacctgttcg gcctggaccg cgaccaactc aacgacatcg cgctgtatct gaacttcacc atgctcggct cgcccaacgc cgggttcttc accgacgacg gcgatcagtc cgggccgccg gcccccggta tttcggccgc cgacgttccc gaagggtcgg ccggcatcga gcgcaccctg gcgggctatc tgaacctggc cgggaagcgt gcggcggaca tgcccctgaa cacccgggcc gactaccacc ccttcatggt ggccggtgtg ccgatcggcg gcatgaccac cggagcctcg caaccgaaaa ccactgtgca gtcccggttg tggggcggcc aggccggcgt gccgttcgat ccgaacttcc aaagcccccg cgacaccgcc gacaacatca accgggacgc cctggcggtg atgggttccg gcgtcgggtt cgcggttggc agctacgccc aatcgatcgg cggggtcaac ggggtgccgc cgcacgacaa gcggcatcgc acccgagtgc ccaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa