MypaA.00896.b

CitA

CENTER ID: MypaA.00896.b
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00896.b.A1.GE29518 Full length( MypaA.00896.b ) 1 390
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.869
RefSeq: NP_959761.2
UniProt: Q742K8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLPAGSVRPR AGCENFPMTV VPENFVPGLE GVVAFTTEIA EPDKDGGALR YRGVDIEDLV SQQVTFGDVW ALLVDGKFGH GLPPAEPFPL PIHTGDVRVD VQAGLAMLAP IWGYKPLLDT DDATARDQLA RASVMALSYV AQSARGIYQP AVPQRVIDEC ETVTARFMTR WQGEPDPRHI EAIDAYWVSA AEHGMNASTF TARVIASTGA DVAAALSGAI GAMSGPLHGG APARVLPMIE EVERTGDARG LVKKILDSGD KLMGFGHRVY RAEDPRARVL RATAERLGAP RHEVAVALEQ AALAELRERR PDRAIETNVE FWAAVILDFA RVPANMMPAM FTCGRTAGWC AHILEQKRLG KLVRPSAIYV GPGPRSPESV EGWDRSLTNA
NT Sequence
TTGCTACCGG CCGGTAGCGT CCGCCCCAGG GCGGGGTGCG AGAATTTTCC CATGACTGTT GTCCCCGAGA ACTTTGTCCC CGGCCTGGAA GGCGTGGTCG CCTTCACCAC CGAAATCGCC GAACCGGATA AAGACGGCGG CGCGCTGCGC TACCGCGGTG TCGACATCGA AGACCTGGTG AGCCAGCAGG TCACGTTCGG CGACGTGTGG GCGCTGCTGG TCGACGGCAA GTTCGGCCAC GGGCTGCCGC CCGCCGAGCC GTTCCCGCTG CCGATCCACA CCGGCGACGT GCGCGTCGAC GTGCAGGCCG GGCTGGCCAT GCTGGCCCCG ATCTGGGGCT ACAAGCCGCT GCTGGACACC GACGACGCCA CCGCCCGCGA CCAGCTGGCC CGGGCCTCGG TGATGGCGCT GTCCTACGTC GCGCAGTCCG CGCGCGGCAT CTATCAGCCC GCGGTGCCGC AGCGGGTCAT CGACGAATGC GAAACCGTCA CTGCACGTTT CATGACGCGG TGGCAGGGCG AGCCGGACCC GCGGCACATC GAGGCGATCG ACGCCTACTG GGTGTCGGCC GCCGAGCACG GCATGAACGC CTCGACGTTC ACCGCGCGGG TGATCGCCTC CACCGGCGCC GACGTGGCGG CCGCGTTGTC CGGGGCGATC GGGGCGATGA GCGGACCGCT GCACGGCGGG GCGCCGGCGC GGGTGCTGCC GATGATCGAG GAGGTCGAGC GCACCGGCGA CGCCCGAGGG CTGGTCAAGA AGATCCTGGA CAGCGGCGAC AAGCTGATGG GCTTCGGGCA CCGGGTCTAC CGCGCCGAGG ATCCCCGCGC GCGGGTGCTG CGCGCCACCG CCGAGCGGCT CGGCGCGCCG CGGCACGAGG TCGCCGTCGC ACTGGAGCAG GCCGCCCTGG CCGAACTGCG CGAGCGCCGT CCGGACCGGG CCATCGAGAC CAACGTCGAG TTCTGGGCGG CGGTCATCCT GGACTTCGCC CGGGTGCCGG CCAACATGAT GCCGGCGATG TTCACCTGTG GCCGCACCGC GGGGTGGTGC GCCCACATTC TCGAGCAGAA GCGGCTCGGC AAGCTGGTCC GCCCGTCGGC GATCTACGTG GGCCCCGGCC CGCGCAGCCC GGAATCCGTC GAGGGTTGGG ACCGCAGCCT CACCAACGCC
Details for MypaA.00896.b.A1.GE29518
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLLPAGSVR PRAGCENFPM TVVPENFVPG LEGVVAFTTE IAEPDKDGGA LRYRGVDIED LVSQQVTFGD VWALLVDGKF GHGLPPAEPF PLPIHTGDVR VDVQAGLAML APIWGYKPLL DTDDATARDQ LARASVMALS YVAQSARGIY QPAVPQRVID ECETVTARFM TRWQGEPDPR HIEAIDAYWV SAAEHGMNAS TFTARVIAST GADVAAALSG AIGAMSGPLH GGAPARVLPM IEEVERTGDA RGLVKXXLXS GXKLMGFGHR VYRAEXPRAR VLXATXXRXG APXXXVPSHW XXXXWXNXXX XXXXXRXXXX XXXXXSXXXX XXXXXXXXXX PXGXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
anattttgtt tactttaaga aggaganata ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatgtt gctaccggcc ggtagcgtcc gccccagggc ggggtgcgag aattttccca tgactgttgt ccccgagaac tttgtccccg gcctggaagg cgtggtcgcc ttcaccaccg aaatcgccga accggataaa gacggcggcg cgctgcgcta ccgcggtgtc gacatcgaag acctggtgag ccagcaggtc acgttcggcg acgtgtgggc gctgctggtc gacggcaagt tcggccacgg gctgccgccc gccgagccgt tcccgctgcc gatccacacc ggcgacgtgc gcgtcgacgt gcaggccggg ctggccatgc tggccccgat ctggggctac aagccgctgc tggacaccga cgacgccacc gcccgcgacc agctggcccg ggcctcggtg atggcgctgt cctacgtcgc gcagtccgcg cgcggcatct atcagcccgc ggtgccgcag cgggtcatcg acgaatgcga aaccgtcact gcacgtttca tgacgcggtg gcagggcgag ccggacccgc ggcacatcga ggcgatcgac gcctactggg tgtcggccgc cgagcacggc atgaacgcct cgacgttcac cgcgcgggtg atcgcctcca ccggcgccga cgtggcggcc gcgttgtccg gggcgatcgg ggcgatgagc ggaccgctgc acggcggggc gccggcgcgg gtgctgccga tgatcgagga ggtcgagcgc accggcgacg cccgagggct ggtcaagaan ancctggnca gcggcganaa gctgatgggc ttcgggcacc gggtctaccg cgccgaggnn ccccgcgcgc gggtgctgng cgccaccgnn gancggntcg gcgcgccnng gnncgangtc ccgtcgcact ggnncangcn gncctggnnn aactgnncna ncnnnncnna nngnnntcga gancangnnn nantnntggn nnngncntcn nnggnnnnnn nnnntnatgn nnnnnnnnnn nncnnnnnnn nnccgnnggg nnnnnnnnnn nnnnnnnnna nnngnnnnnn nnnnnnnnnn cnnnccnnnc nnnnnnnntn nnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLLPAGSVR PRAGCENFPM TVVPENFVPG LEGVVAFTTE IAEPDKDGGA LRYRGVDIED LVSQQVTFGD VWALLVDGKF GHGLPPAEPF PLPIHTGDVR VDVQAGLAML APIWGYKPLL DTDDATARDQ LARASVMALS YVAQSARGIY QPAVPQRVID ECETVTARFM TRWQGEPDPR HIEAIDAYWV SAAEHGMNAS TFTARVIAST GADVAAALSG AIGAMSGPLH GGAPARVLPM IEEVERTGDA RGLVKKILDS GDKLMGFGHR VYRAEDPRAR VLRATAERLG APRHEVAVAL EQAALAELRE RRPDRAIETN VEFWAAVILD FARVPANMMP AMFTCGRTAG WCAHILEQKR LGKLVRPSAI YVGPGPRSPE SVEGWDRSLT NA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgctaccg gccggtagcg tccgccccag ggcggggtgc gagaattttc ccatgactgt tgtccccgag aactttgtcc ccggcctgga aggcgtggtc gccttcacca ccgaaatcgc cgaaccggat aaagacggcg gcgcgctgcg ctaccgcggt gtcgacatcg aagacctggt gagccagcag gtcacgttcg gcgacgtgtg ggcgctgctg gtcgacggca agttcggcca cgggctgccg cccgccgagc cgttcccgct gccgatccac accggcgacg tgcgcgtcga cgtgcaggcc gggctggcca tgctggcccc gatctggggc tacaagccgc tgctggacac cgacgacgcc accgcccgcg accagctggc ccgggcctcg gtgatggcgc tgtcctacgt cgcgcagtcc gcgcgcggca tctatcagcc cgcggtgccg cagcgggtca tcgacgaatg cgaaaccgtc actgcacgtt tcatgacgcg gtggcagggc gagccggacc cgcggcacat cgaggcgatc gacgcctact gggtgtcggc cgccgagcac ggcatgaacg cctcgacgtt caccgcgcgg gtgatcgcct ccaccggcgc cgacgtggcg gccgcgttgt ccggggcgat cggggcgatg agcggaccgc tgcacggcgg ggcgccggcg cgggtgctgc cgatgatcga ggaggtcgag cgcaccggcg acgcccgagg gctggtcaag aagatcctgg acagcggcga caagctgatg ggcttcgggc accgggtcta ccgcgccgag gatccccgcg cgcgggtgct gcgcgccacc gccgagcggc tcggcgcgcc gcggcacgag gtcgccgtcg cactggagca ggccgccctg gccgaactgc gcgagcgccg tccggaccgg gccatcgaga ccaacgtcga gttctgggcg gcggtcatcc tggacttcgc ccgggtgccg gccaacatga tgccggcgat gttcacctgt ggccgcaccg cggggtggtg cgcccacatt ctcgagcaga agcggctcgg caagctggtc cgcccgtcgg cgatctacgt gggccccggc ccgcgcagcc cggaatccgt cgagggttgg gaccgcagcc tcaccaacgc caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa