MypaA.00829.a

EchA2

CENTER ID: MypaA.00829.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00829.a.A1.GE29513 Full length( MypaA.00829.a ) 1 303
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00829.a.A1.PS00755 Full length( MypaA.00829.a ) 1 303

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.4201
RefSeq: NP_962883.1
UniProt: Q73SX4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSDFETLLY TTAGPVATIT LNRPEQLNTI VPPMPDEIEA AVGRAERDPA IKVIVLRGAG RAFSGGYDFG GGFQHWGEAM MTDGRWDPGK DFAMVSARET GPTQKFMAIW RASKPVIAQV HGWCVGGASD YALCADLVIA SEDAVIGTPY SRMWGAYLTG MWLYRLSLAK AKWHALTGRP LTGVQAAEIE LINEAVPFER LEARVAEVAA ELARIPLSQL QAQKLIVNQA YENMGLASTQ TLGGILDGLM RNTPDALEFI KIAETRGVRA AVERRDGPFG DYSQAPPELR PDPSHVIVPD RDG
NT Sequence
ATGCCCTCCG ACTTCGAAAC GCTGCTGTAC ACCACGGCCG GTCCGGTCGC CACGATCACG CTGAACCGCC CGGAGCAGCT CAACACCATC GTGCCGCCGA TGCCCGACGA GATCGAGGCC GCCGTTGGGC GGGCCGAGCG TGACCCGGCC ATCAAGGTCA TCGTGCTGCG CGGAGCGGGC CGGGCCTTCT CCGGCGGCTA CGACTTCGGC GGCGGTTTTC AGCACTGGGG CGAGGCCATG ATGACCGACG GCCGCTGGGA CCCGGGCAAG GATTTCGCGA TGGTCAGCGC CCGCGAGACC GGCCCGACCC AGAAATTCAT GGCCATCTGG CGGGCGTCCA AACCGGTGAT CGCGCAGGTG CACGGCTGGT GTGTGGGCGG GGCCAGCGAC TACGCGCTGT GCGCGGACCT GGTCATCGCC AGCGAGGACG CCGTGATCGG CACGCCCTAC AGCAGGATGT GGGGGGCGTA TCTGACCGGG ATGTGGCTCT ACCGGCTGAG CCTGGCCAAG GCGAAGTGGC ATGCGCTGAC GGGACGGCCG CTGACCGGCG TGCAGGCCGC CGAGATCGAG CTGATCAACG AGGCGGTGCC GTTCGAGCGG CTGGAGGCGC GGGTCGCCGA GGTCGCCGCC GAGCTGGCGC GCATCCCGCT GTCGCAGCTG CAGGCGCAGA AGCTGATCGT CAACCAGGCC TACGAGAACA TGGGGCTGGC GTCCACCCAG ACGCTGGGCG GCATCCTCGA CGGGCTGATG CGCAACACCC CCGACGCGCT GGAGTTCATC AAGATCGCCG AGACGCGGGG GGTGCGGGCC GCCGTCGAGC GCCGCGACGG CCCGTTCGGC GACTACAGCC AGGCCCCGCC GGAGCTGCGG CCCGATCCGA GCCACGTCAT CGTCCCTGAT CGCGATGGA
Details for MypaA.00829.a.A1.GE29513
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Validated AA Sequence
MPSDFETLLY TTAGPVATIT LNRPEQLNTI VPPMPDEIEA AVGRAERDPA IKVIVLRGAG RAFSGGYDFG GGFQHWGEAM MTDGRWDPGK DFAMVSARET GPTQKFMAIW RASKPVIAQV HGWCVGGASD YALCADLVIA SEDAVIGTPY SRMWGAYLTG MWLYRLSLAK AKWHALTGRP LTGVQAAEIE LINEAVPFER LEARVAEVAA ELARIPLSQL QAQKLIVNQA YENMGLASTQ TLGGILDGLM RNTPDALEFI KIAETRGVRA AVERRDGPFG DYSQAPPELR PDPSHVIVPD R
Validated NT Sequence
cgcgatcagg gacgatgacg tggctcggat cgggccgcag ctccggcggg gcctggctgt agtcgccgaa cgggccgtcg cggcgctcga cggcggcccg caccccccgc gtctcggcga tcttgatgaa ctccagcgcg tcgggggtgt tgcgcatcag cccgtcgagg atgccgccca gcgtctgggt ggacgccagc cccatgttct cgtaggcctg gttgacgatc agcttctgcg cctgcagctg cgacagcggg atgcgcgcca gctcggcggc gacctcggcg acccgcgcct ccagccgctc gaacggcacc gcctcgttga tcagctcgat ctcggcggcc tgcacgccgg tcagcggccg tcccgtcagc gcatgccact tcgccttggc caggctcagc cggtagagcc acatcccggt cagatacgcc ccccacatcc tgctgtaggg cgtgccgatc acggcgtcct cgctggcgat gaccaggtcc gcgcacagcg cgtagtcgct ggccccgccc acacaccagc cgtgcacctg cgcgatcacc ggtttggacg cccgccagat ggccatgaat ttctgggtcg ggccggtctc gcgggcgctg accatcgcga aatccttgcc cgggtcccag cggccgtcgg tcatcatggc ctcgccccag tgctgaaaac cgccgccgaa gtcgtagccg ccggagaagg cccggcccgc tccgcgcagc acgatgacct tgatggccgg gtcacgctcg gcccgcccaa cggcggcctc gatctcgtcg ggcatcggcg gcacgatggt gttgagctgc tccgggcggt tcagcgtgat cgtggcgacc ggaccggccg tggtgtacag cagcgtttcg aagtcggagg gcatcgaacc aggaccctgg gtctgagctt cca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPSDFETLL YTTAGPVATI TLNRPEQLNT IVPPMPDEIE AAVGRAERDP AIKVIVLRGA GRAFSGGYDF GGGFQHWGEA MMTDGRWDPG KDFAMVSARE TGPTQKFMAI WRASKPVIAQ VHGWCVGGAS DYALCADLVI ASEDAVIGTP YSRMWGAYLT GMWLYRLSLA KAKWHALTGR PLTGVQAAEI ELINEAVPFE RLEARVAEVA AELARIPLSQ LQAQKLIVNQ AYENMGLAST QTLGGILDGL MRNTPDALEF IKIAETRGVR AAVERRDGPF GDYSQAPPEL RPDPSHVIVP DRDG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccctccgac ttcgaaacgc tgctgtacac cacggccggt ccggtcgcca cgatcacgct gaaccgcccg gagcagctca acaccatcgt gccgccgatg cccgacgaga tcgaggccgc cgttgggcgg gccgagcgtg acccggccat caaggtcatc gtgctgcgcg gagcgggccg ggccttctcc ggcggctacg acttcggcgg cggttttcag cactggggcg aggccatgat gaccgacggc cgctgggacc cgggcaagga tttcgcgatg gtcagcgccc gcgagaccgg cccgacccag aaattcatgg ccatctggcg ggcgtccaaa ccggtgatcg cgcaggtgca cggctggtgt gtgggcgggg ccagcgacta cgcgctgtgc gcggacctgg tcatcgccag cgaggacgcc gtgatcggca cgccctacag caggatgtgg ggggcgtatc tgaccgggat gtggctctac cggctgagcc tggccaaggc gaagtggcat gcgctgacgg gacggccgct gaccggcgtg caggccgccg agatcgagct gatcaacgag gcggtgccgt tcgagcggct ggaggcgcgg gtcgccgagg tcgccgccga gctggcgcgc atcccgctgt cgcagctgca ggcgcagaag ctgatcgtca accaggccta cgagaacatg gggctggcgt ccacccagac gctgggcggc atcctcgacg ggctgatgcg caacaccccc gacgcgctgg agttcatcaa gatcgccgag acgcgggggg tgcgggccgc cgtcgagcgc cgcgacggcc cgttcggcga ctacagccag gccccgccgg agctgcggcc cgatccgagc cacgtcatcg tccctgatcg cgatggaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MypaA.00829.a.A1.PS00755
PURIFICATION DATe: 9/2/2010
CONCENTRATION: 45.56mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Protocol Notes
notes unavailable
Validated AA Sequence
MPSDFETLLY TTAGPVATIT LNRPEQLNTI VPPMPDEIEA AVGRAERDPA IKVIVLRGAG RAFSGGYDFG GGFQHWGEAM MTDGRWDPGK DFAMVSARET GPTQKFMAIW RASKPVIAQV HGWCVGGASD YALCADLVIA SEDAVIGTPY SRMWGAYLTG MWLYRLSLAK AKWHALTGRP LTGVQAAEIE LINEAVPFER LEARVAEVAA ELARIPLSQL QAQKLIVNQA YENMGLASTQ TLGGILDGLM RNTPDALEFI KIAETRGVRA AVERRDGPFG DYSQAPPELR PDPSHVIVPD R
Validated NT Sequence
cgcgatcagg gacgatgacg tggctcggat cgggccgcag ctccggcggg gcctggctgt agtcgccgaa cgggccgtcg cggcgctcga cggcggcccg caccccccgc gtctcggcga tcttgatgaa ctccagcgcg tcgggggtgt tgcgcatcag cccgtcgagg atgccgccca gcgtctgggt ggacgccagc cccatgttct cgtaggcctg gttgacgatc agcttctgcg cctgcagctg cgacagcggg atgcgcgcca gctcggcggc gacctcggcg acccgcgcct ccagccgctc gaacggcacc gcctcgttga tcagctcgat ctcggcggcc tgcacgccgg tcagcggccg tcccgtcagc gcatgccact tcgccttggc caggctcagc cggtagagcc acatcccggt cagatacgcc ccccacatcc tgctgtaggg cgtgccgatc acggcgtcct cgctggcgat gaccaggtcc gcgcacagcg cgtagtcgct ggccccgccc acacaccagc cgtgcacctg cgcgatcacc ggtttggacg cccgccagat ggccatgaat ttctgggtcg ggccggtctc gcgggcgctg accatcgcga aatccttgcc cgggtcccag cggccgtcgg tcatcatggc ctcgccccag tgctgaaaac cgccgccgaa gtcgtagccg ccggagaagg cccggcccgc tccgcgcagc acgatgacct tgatggccgg gtcacgctcg gcccgcccaa cggcggcctc gatctcgtcg ggcatcggcg gcacgatggt gttgagctgc tccgggcggt tcagcgtgat cgtggcgacc ggaccggccg tggtgtacag cagcgtttcg aagtcggagg gcatcgaacc aggaccctgg gtctgagctt cca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPSDFETLL YTTAGPVATI TLNRPEQLNT IVPPMPDEIE AAVGRAERDP AIKVIVLRGA GRAFSGGYDF GGGFQHWGEA MMTDGRWDPG KDFAMVSARE TGPTQKFMAI WRASKPVIAQ VHGWCVGGAS DYALCADLVI ASEDAVIGTP YSRMWGAYLT GMWLYRLSLA KAKWHALTGR PLTGVQAAEI ELINEAVPFE RLEARVAEVA AELARIPLSQ LQAQKLIVNQ AYENMGLAST QTLGGILDGL MRNTPDALEF IKIAETRGVR AAVERRDGPF GDYSQAPPEL RPDPSHVIVP DRDG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCCTCCGAC TTCGAAACGC TGCTGTACAC CACGGCCGGT CCGGTCGCCA CGATCACGCT GAACCGCCCG GAGCAGCTCA ACACCATCGT GCCGCCGATG CCCGACGAGA TCGAGGCCGC CGTTGGGCGG GCCGAGCGTG ACCCGGCCAT CAAGGTCATC GTGCTGCGCG GAGCGGGCCG GGCCTTCTCC GGCGGCTACG ACTTCGGCGG CGGTTTTCAG CACTGGGGCG AGGCCATGAT GACCGACGGC CGCTGGGACC CGGGCAAGGA TTTCGCGATG GTCAGCGCCC GCGAGACCGG CCCGACCCAG AAATTCATGG CCATCTGGCG GGCGTCCAAA CCGGTGATCG CGCAGGTGCA CGGCTGGTGT GTGGGCGGGG CCAGCGACTA CGCGCTGTGC GCGGACCTGG TCATCGCCAG CGAGGACGCC GTGATCGGCA CGCCCTACAG CAGGATGTGG GGGGCGTATC TGACCGGGAT GTGGCTCTAC CGGCTGAGCC TGGCCAAGGC GAAGTGGCAT GCGCTGACGG GACGGCCGCT GACCGGCGTG CAGGCCGCCG AGATCGAGCT GATCAACGAG GCGGTGCCGT TCGAGCGGCT GGAGGCGCGG GTCGCCGAGG TCGCCGCCGA GCTGGCGCGC ATCCCGCTGT CGCAGCTGCA GGCGCAGAAG CTGATCGTCA ACCAGGCCTA CGAGAACATG GGGCTGGCGT CCACCCAGAC GCTGGGCGGC ATCCTCGACG GGCTGATGCG CAACACCCCC GACGCGCTGG AGTTCATCAA GATCGCCGAG ACGCGGGGGG TGCGGGCCGC CGTCGAGCGC CGCGACGGCC CGTTCGGCGA CTACAGCCAG GCCCCGCCGG AGCTGCGGCC CGATCCGAGC CACGTCATCG TCCCTGATCG CGATGGAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA