SSGCID: Target Page

MypaA.00750.a

Phosphate import ATP-binding protein pstB

CENTER ID:	MypaA.00750.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00750.a.A1.GE29482	Full length( MypaA.00750.a )		1	258

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.689
RefSeq:	NP_959588.1
UniProt:	Q743D1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKRLDLKGV NIYYGSFHAV AEVTLSVLPR SVTAFIGPSG CGKTTVLRTL NRMHEVVPGG RVEGSLLLDD EDIYGPGVDP VGVRRAIGMV FQRPNPFPAM SIRDNVVAGL KLQGVRNRKV LDETAEYSLR GANLWDEVKD RLDRPGGGLS GGQQQRLCIA RAIAVQPDVL LMDEPCSALD PISTMAIEEL ISELKQDYTI VIVTHNMQQA ARVSDYTAFF NLEAVGKPGR LIEVDDTEKI FSNPSQKATE DYISGRFG

NT Sequence

GTGGCCAAAC GGTTGGACCT CAAGGGTGTC AACATCTACT ACGGGTCGTT TCACGCCGTC GCGGAGGTGA CGCTGTCGGT GCTTCCGCGC AGCGTCACGG CGTTCATCGG CCCGTCGGGT TGCGGCAAGA CGACGGTGTT GCGCACCCTC AACCGGATGC ACGAGGTGGT GCCCGGCGGG CGGGTCGAGG GAAGCTTGCT GCTCGACGAC GAAGACATCT ACGGCCCCGG TGTCGACCCT GTCGGGGTGC GCCGGGCCAT CGGCATGGTG TTCCAGCGGC CGAACCCGTT CCCCGCCATG TCAATTCGCG ACAACGTGGT CGCGGGCCTG AAGCTGCAAG GGGTCCGCAA CCGCAAGGTG CTCGACGAGA CGGCCGAGTA CTCGCTGCGC GGCGCCAACC TGTGGGACGA GGTCAAGGAC CGGCTGGACA GGCCCGGCGG CGGACTCTCG GGCGGGCAGC AGCAGCGGTT GTGCATCGCG CGGGCCATCG CGGTGCAACC GGATGTGCTG CTGATGGACG AGCCGTGCTC GGCGCTGGAC CCGATTTCGA CGATGGCCAT CGAAGAGCTG ATCAGCGAGC TGAAGCAGGA CTACACCATC GTCATCGTCA CCCACAACAT GCAGCAGGCC GCCCGGGTCA GCGACTACAC CGCGTTTTTC AACCTGGAAG CCGTCGGAAA GCCGGGACGA CTGATCGAGG TCGACGACAC CGAGAAGATC TTCTCCAACC CCAGCCAGAA GGCGACGGAG GACTACATCT CGGGCCGCTT CGGT

Details for MypaA.00750.a.A1.GE29482

HARVESTED ON:	5/27/2010
SEQUENCED ON:	6/14/2010
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVAKRLDLK GVNIYYGSFH AVAEVTLSVL PRSVTAFIGP SGCGKTTVLR TLNRMHEVVP GGRVEGSLLL DDEDIYGPGV DPVGVRRAIG MVFQRPNPFP AMSIRDNVVA GLKLQGVRNR KVLDETAEYS LRGANLWDEV KDRLDRPGGG LSGGQQQRLC IARAIAVQPD VLLMDEPCSA LDPISTMAIE ELISELKQDY TIVIVTHNMQ QAARVSDYTA FFNLEAVGKP GRLIEVDXTX KIFSNPSQXA TXXYXSGRFG

Validated NT Sequence

tttntttact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtggcc aaacggttgg acctcaaggg tgtcaacatc tactacgggt cgtttcacgc cgtcgcggag gtgacgctgt cggtgcttcc gcgcagcgtc acggcgttca tcggcccgtc gggttgcggc aagacgacgg tgttgcgcac cctcaaccgg atgcacgagg tggtgcccgg cgggcgggtc gagggaagct tgctgctcga cgacgaagac atctacggcc ccggtgtcga ccctgtcggg gtgcgccggg ccatcggcat ggtgttccag cggccgaacc cgttccccgc catgtcaatt cgcgacaacg tggtcgcggg cctgaagctg caaggggtcc gcaaccgcaa ggtgctcgac gagacggccg agtactcgct gcgcggcgcc aacctgtggg acgaggtcaa ggaccggctg gacaggcccg gcggcggact ctcgggcggg cagcagcagc ggttgtgcat cgcgcgggcc atcgcggtgc aaccggatgt gctgctgatg gacgagccgt gctcggcgct ggacccgatt tcgacgatgg ccatcgaaga gctgatcagc gagctgaagc aggactacac catcgtcatc gtcacccaca acatgcagca ggccgcccgg gtcagcgact acaccgcgtt tttcaacctg gaagccgtcg gaaagccggg acgactgatc gaggtcgacn acaccganaa gatcttctcc aaccccagcc agaangcgac ggangantac ntctcgggcc gcttcggtta ataaacagca cgaacaagtt ctgcagccaa gcttctcgag natnnngctg ctaacaaagc ccnaaaggaa gctgagttgg ctgctgccnc cgctgannaa nnnanna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccaaa cggttggacc tcaagggtgt caacatctac tacgggtcgt ttcacgccgt cgcggaggtg acgctgtcgg tgcttccgcg cagcgtcacg gcgttcatcg gcccgtcggg ttgcggcaag acgacggtgt tgcgcaccct caaccggatg cacgaggtgg tgcccggcgg gcgggtcgag ggaagcttgc tgctcgacga cgaagacatc tacggccccg gtgtcgaccc tgtcggggtg cgccgggcca tcggcatggt gttccagcgg ccgaacccgt tccccgccat gtcaattcgc gacaacgtgg tcgcgggcct gaagctgcaa ggggtccgca accgcaaggt gctcgacgag acggccgagt actcgctgcg cggcgccaac ctgtgggacg aggtcaagga ccggctggac aggcccggcg gcggactctc gggcgggcag cagcagcggt tgtgcatcgc gcgggccatc gcggtgcaac cggatgtgct gctgatggac gagccgtgct cggcgctgga cccgatttcg acgatggcca tcgaagagct gatcagcgag ctgaagcagg actacaccat cgtcatcgtc acccacaaca tgcagcaggc cgcccgggtc agcgactaca ccgcgttttt caacctggaa gccgtcggaa agccgggacg actgatcgag gtcgacgaca ccgagaagat cttctccaac cccagccaga aggcgacgga ggactacatc tcgggccgct tcggtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa